Microbial composition for promoting tobacco growth and use

A microbial composition and application technology, applied in the field of microorganisms, can solve problems such as unfriendly environment, secondary pollution, etc., and achieve the effects of improving the effective leaf number, improving the microbial flora, and promoting the growth effect of tobacco

Active Publication Date: 2017-02-22
四川省食用菌研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of most chemical fertilizers is not friendly to the environment and is likely to cause secondary pollution. Therefore, biological fertilizers are more and more popular due to their good environmental friendliness. Among them, microbial fertilizers are more because of their short cultivation time and easy control of cultivation conditions. and other advantages have become the focus of attention, and its application prospects in promoting tobacco growth are getting better and better

Method used

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  • Microbial composition for promoting tobacco growth and use
  • Microbial composition for promoting tobacco growth and use
  • Microbial composition for promoting tobacco growth and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, the screening of optimal microbial composition obtains

[0035] 1.1 Determination method of IAA

[0036] Isolation and screening of 5 strains of bacteria from the soil have a strong ability to produce IAA, these 5 strains are: 1.YBT-003-B-5; 2.YB-001-B-8; 3.FY-001-B -9; 4. MT-002-B-12; 5. L2-001-B-16. Composite construction was carried out on 5 strains.

[0037] (1) Preparation of standard curve

[0038] Configure the IAA standard solution with a concentration of 0, 10, 20, 30, 40, 50, 60ug / mL, and mix it with Salkowski colorimetric solution (50mL 35% HClO 4 +1mL 0.5M FeCl 3 ) in a 1:1 (v / v) ratio, placed at room temperature in the dark for 30 min, and then measured the absorbance of each concentration at 530 nm (the 1:1 mixed solution of distilled water and Salkowski colorimetric solution was used as a blank control). Finally, the IAA standard curve was drawn with the IAA concentration as the abscissa and the absorbance as the ordinate, and the regre...

Embodiment 2

[0050] Embodiment two. The following are field test examples

[0051] 1. Preparation method of microbial composition fermented liquid

[0052] According to Example 1, the 4 strains in "1345" were combined to configure a composite strain. Inoculate one loop of each of the four strains into a 500ml Erlenmeyer flask filled with 250ml LB culture medium, ferment and culture in a shaking table at 30°C and 180r / min for 48h to form a seed solution. Utilize fermentor to carry out amplified culture to seed liquid, fermentation condition is that fermentation time is 36h, ventilation rate 0.5V / V.M, rotating speed 250r / h, temperature 30 ℃, and the ready-made composition is fermented liquid.

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Abstract

The invention belongs to the field of microorganisms, and in particular provides a microbial composition for promoting tobacco growth. The microbial composition is formed by compounding specific Bacillus tequilensis, Enterobacter cloacae, Klebsiella variicola and Enterobacter xiangfangensis strains at a ratio. The microbial composition can effectively improve agronomic characters of tobacco, adjust the proportion of beneficial microorganisms in the soil, and increase the yield of tobacco and the proportion of superior tobacco.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a microbial composition and its growth-promoting effect on tobacco. [0002] technical background: [0003] Tobacco is one of the important economic crops in my country. As of 2010, tobacco planting area was 1.35 million hectares, accounting for 0.86% of the total cultivated area. There are a wide range of tobacco varieties in my country, and as far as the main tobacco cultivation distribution areas are concerned, the tobacco leaf production areas are concentrated in the economically underdeveloped areas of Yunnan, Guizhou, Sichuan, Chongqing, Hunan and other places, and the economic benefits brought by flue-cured tobacco to farmers are far greater than other crops Therefore, tobacco leaf has become an important economic crop in tobacco growing areas, and tobacco leaf production has become a pillar planting industry in these planting areas and a pillar income method for farmers, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/07C12R1/22C12R1/01
Inventor 吴翔甘炳成黄忠乾谢丽源彭卫红谭昊谭伟李小林唐杰
Owner 四川省食用菌研究所
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