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Enterobacter cloacae, microbial inoculum containing enterobacter cloacae, use of microbial inoculum and method for passivation of mercury

A technology of Enterobacter cloacae and bacteria agent, applied in the field of bioremediation of heavy metal mercury pollution, to achieve the effect of environmental friendliness, easy operation and simple operation

Inactive Publication Date: 2017-03-15
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Enterobacter cloacae, the bacterium agent containing this bacterium and its application and a kind of method of passivating mercury in order to overcome the above-mentioned defect that exists in mercury pollution bioremediation

Method used

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  • Enterobacter cloacae, microbial inoculum containing enterobacter cloacae, use of microbial inoculum and method for passivation of mercury
  • Enterobacter cloacae, microbial inoculum containing enterobacter cloacae, use of microbial inoculum and method for passivation of mercury
  • Enterobacter cloacae, microbial inoculum containing enterobacter cloacae, use of microbial inoculum and method for passivation of mercury

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Embodiment

[0036] The present invention will be described in detail below through examples and comparative examples, but the present invention is not limited thereby.

[0037] The experimental methods in the following examples and comparative examples are conventional methods in the art unless otherwise specified. The experimental materials used in the following examples and comparative examples were purchased from conventional biochemical reagent stores unless otherwise specified.

[0038] Mercury degradation rate = (Hg before treatment 2+ Content - Hg after treatment 2+ content) / Hg before treatment 2+ Content × 100%.

[0039] The cold atomic absorption mercury analyzer was purchased from Beijing Liman Technology Co., Ltd., model Hydra AAAuto.

Embodiment 1

[0043] This example is used to illustrate the tolerance and passivation ability of Enterobacter cloacae with the preservation number of CGMCC NO: 10850 to mercury in the water phase of the present invention

[0044] In 200ml of LB liquid medium, add 1% by volume of the bacterial solution obtained in the preparation example, and add a certain amount of HgCl 2 , so that the final concentrations were 5mg / L, 15mg / L, 30mg / L and 50mg / L respectively. Cultivate in a constant temperature oscillator at 30°C and 170rpm, and take samples at regular intervals to determine the OD of bacteria 600 1. Use a cold atomic absorption mercury analyzer to measure the residual mercury and calculate the mercury degradation rate. Different Hg at 48h 2+ The results of mercury removal rate under the concentration are shown in Table 1, different Hg 2+ The growth status of the strain at the concentration is as follows figure 1 shown. It can be seen from Table 1 that at 48h, Hg 2+ When the concentrati...

Embodiment 2

[0048] This embodiment is used to illustrate that the preservation number of the present invention is the passivation effect of Enterobacter cloacae of CGMCC NO: 10850 on mercury in soil

[0049] Sterilize 100ml of LB liquid medium at 121°C for 15min, then add 1% by volume of the bacterial solution obtained in the preparation example, and incubate at 170rpm, 30±1°C for 12h. The aforementioned bacterial solution was added to 1kg of mercury-contaminated soil (Hg 2+ Content is 25mg / kg), and add sterile deionized water, mix evenly, cultivate for 15 days, and ensure that the water content of the soil is 20% every day. After 15 days, soil samples were taken and dried at 40°C, ground into a uniform powder, and the final residual mercury was determined by a cold atomic absorption mercury analyzer. The results showed that Hg in the soil 2+ From the initial 25mg / kg to 5.47mg / kg, it shows that the strain can be effective on Hg in soil 2+ It produces a strong degradation effect, thereb...

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Abstract

The invention relates to the technical field of bioremediation of heavy metal mercury pollution and discloses enterobacter cloacae, a microbial inoculum containing the enterobacter cloacae, a use of the microbial inoculum and a method for passivation of mercury. The enterobacter cloacae has an accession number of CGMCC NO: 10850. The method for passivation of mercury comprises contacting the enterobacter cloacae having the accession number of CGMCC NO: 10850 and / or the microbial inoculum containing the enterobacter cloacae having the accession number of CGMCC NO: 10850 with a mercury-polluted sample so that mercury in the mercury-polluted sample is passivated. The enterobacter cloacae has high degradation ability to mercury, has a degradation rate of 85.5%, can tolerate high concentration mercury pollution, is easy to operate and can play an important role in bioremediation of mercury-polluted soil or mercury-containing wastewater.

Description

technical field [0001] The invention relates to the technical field of bioremediation of mercury pollution by heavy metals, in particular to an enterobacter cloacae, a bacterial agent containing the bacteria and its application, and a mercury passivation method. Background technique [0002] Mercury (Hg), also known as mercury, has a melting point of only -38.87°C and is the only metal that is liquid and easy to flow at room temperature. Mercury and its compounds are very important industrial raw materials, usually used as catalysts or raw materials, and widely used in chemical, paint, pharmaceutical, agricultural and other industries. Global mercury levels continue to increase due to human activities due to the extensive use of mercury. The Global Mercury Status Assessment states that mercury levels in the atmosphere, water and soil environments have nearly tripled globally since the Industrial Revolution. About 6,500 tons of mercury are released into the environment ever...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/20
Inventor 林森李晓桐任立伟王培红
Owner 粮华生物科技(北京)有限公司
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