Method for quickly identifying bloodstream infection pathogenic bacteria

Abstract

The invention belongs to the field of examination of microorganisms, and relates to a method for quickly identifying bloodstream infection bacteria by a high-resolution fusion curve method. The method comprises the following steps of: performing smear gram staining on a blood culture positive culture flask to distinguish negative bacteria and positive bacteria; selectively amplifying different 16S fragments for the negative bacteria and the positive bacteria; and analyzing by the high-resolution fusion curve method. Common bloodstream infection pathogenic bacteria comprise staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae, Serratia marcescens, Klebsiella oxytoca, acinetobacter lwoffii, enterobacter aerogenes, stenotrophomonas maltophilia and burkholderia cepacia. By the method, the common bloodstream infection pathogenic bacteria can be measured quickly, efficiently and accurately, so that strong evidences are provided and precious time is striven for subsequent treatment.

Description

technical field [0001] The invention belongs to the field of microbiological testing, and relates to a microbiological molecular biological detection method, in particular to rapid identification of bacteria in bloodstream infection by using a high-resolution melting curve method. Background technique [0002] Bloodstream infection is a serious infectious disease, including sepsis and bacteremia. The morbidity rate is high and the case fatality rate is high. In the United States, 750,000 people develop the disease every year, of which about 215,000 people die. It is one of the top ten causes of death, accounting for 6% of all causes of death. Due to the increase in diabetes and cancer patients, the widespread use of invasive procedures, the use of hormones, chemotherapy, and drugs, and the prevalence of multidrug-resistant bacteria, the incidence of bloodstream infections has increased year by year, which has become a global problem. [0003] The traditional diagnosis ...

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However, sequencing and chip technology require expensive special instruments, which are expensive and are not conducive to popularization

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