134 results about "Aerobacter cloacae" patented technology

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The subject matter of the present invention is a bacteria or mix of bacteria having an antagonistic activity with respect to stains of S. aureus, P. aeruginosa, Streptococcus pyogenes, Enterococcus faecium, Enterobacter cloacae, Proteus mirabilis, Bacteroides fragilis Staphylococcus epidermidis, Propionibacterium acnes, Candida albicans and/or Malassezia furfur as well as the use thereof in the treatment and/or prevention of infections or colonisations related to those pathogens. The invention pertains to care products containing one or more non-pathogenic antagonistic strains intended to prevent and/or treat infections or colonisations on skin, wounds, mucous membranes and appendages.

The invention relates to a micro-capsulated suspended microbial seed coating agent and a preparation method thereof. The micro-capsulated suspended microbial seed coating agent comprises a functional bacteria microcapsule and auxiliaries, wherein the functional bacteria microcapsule is prepared from wall materials such as sodium alginate and gelatin by virtue of an endogenous emulsion process, the grain size of the microcapsule is 20-60 mu m, the content of functional bacteria is not lower than 10<9>cfu/g, and the embedding rate is over 80%. Functional microbes are selected from two or more of pseudomonas, bacillus subtilis, bacillus amyloliquefaciens, enterobacter cloacae, raoultella planticola and bacillus atrophaeus. The auxiliaries comprise one or more of a film-forming agent, a dispersant, a tackifier, an anti-freezing agent and a coloring agent. The prepared functional bacteria microcapsule is uniformly mixed with the auxiliaries to obtain a finished product. The product has the advantages of good film-forming performances, thallus persistence, high survivability, good slow-controlled release, strong application stability, environment friendliness, convenience in use, and the like, can be used for promoting seed germination and seedling growth of cottons, soya beans, wheat and potatoes, and the like, controlling the diseases at the seedling stage, improving stress resistance, and the like.

The invention relates to a multifunctional biological seed coating agent and a preparation method thereof. The multifunctional biological seed coating agent comprises auxiliary agents and beneficial microorganism, volume ratio thereof is 1:1-5, and effective bacterium content is not less than or equal to 10<8>cfu/ml. The auxiliary agent comprises one or various auxiliary agents in film former, dispersant, adhesion agent, protectant, nutritient, anti-freeze agent, preservative and colourant. The beneficial microorganism is selected from one or various rhizobium, azotobacter, pseudomonad, bacillus, K pneumoniae, Enterobacter cloacae and Raoulterlla planticola. The preparation method comprises the following steps: dissolving the polyvinyl alcohol and natural substance with water in a reaction kettle, mixing with the water dispersion liquid of the adhesion agent to prepare a film forming agent, and finally mixing and stirring mechanically with the beneficial microorganism fermentation liquor to obtain the finished product. The invention has the advantages of no residue, high efficiency, low toxicity, strong specificity, good film forming effect and convenient application, therefore, the multifunctional biological seed coating agent plays an significant role of preventing and controlling cotton seedling diseases as well as enhancing the cotton seedling growth under salt stress.

The invention discloses enterobacter cloacae and application of the enterobacter cloacae. The enterobacter cloacae SXH-1 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6296. The invention also protects the application of the enterobacter cloacae SXH-1 to the plant growth promotion. The plant is concretely chives. The enterobacter cloacae SXH-1 provided by the invention can use ACC (aminocyclopropane carboxylic acid) as the unique nitrogen source for growth, and meanwhile, the ACC is decomposed. The strain is hopeful to realize important effects in the chive growth promotion aspect.

The invention belongs to the technical field of environmental engineering and microbiology engineering, and particularly relates to a strain for grading PAHs and an application thereof in combined contaminated soil remediation. The strain is an enterobacter cloacae tzyx2 with the collection number of CCTCC NO: M 2012240 in the China Center for Type Culture Collection (CCTCC), and is provided with the capacity to degrade the PAHs. The strain is applied to treatment of the PAHs and heavy metal polluted soil.

The invention relates to degrading bacteria capable of efficiently degrading pesticide chlorothalonil, and belongs to the field of biotechnology. A classification name of the degrading bacteria is Enterobacter cloacae B9; and a culture preservation number is CGMCCNo. 5527. Chlorothalonil is an organic chlorine farm-oriented broad spectrum bactericide and is proved to have very strong effects of carcinogenicity, teratogenicity and mutagenicity. Micro-biological degradation gains more and more significance because of rich bacteria resources in the environment without secondary pollution caused by introducing other chemical reagents. Microbe BJQ2 capable of degrading the chlorothalonil can be obtained by directional culture. The Microbe BJQ2 is identified as the Enterobacter cloacae B9 by morphology, physiological and biochemical analysis and 16SrDNA sequence homology alignment. The bacteria are the degrading bacteria by using the chlorothalonil as a sole carbon source. A degrading rate is 79.2% after being cultured for 6 days in an inorganic salt culture liquid; and a degrading rate is 92.3% after being used in a soil for 7 days. The degrading bacteria can be used for degradation of the bactericide chlorothalonil, and biological purification of water bodies, soil or agricultural products which are polluted by the chlorothalonil.

The invention relates to a primer for detection, specifically relates to a PCR (polymerase chain reaction) detection primer for detecting enterobacter cloacae and belongs to the technical field of biological detection. The enterobacter cloacae specific PCR detection primer comprises a forward primer and a reverse primer, wherein the forward primer F is 5'-CATGACACCGGTGTTTCCCCAGT-3'; and the reverse primer R is 5'-CGGTCGGTGAAGCCCAGAACCACTA-3'. A detection method for detecting the enterobacter cloacae, disclosed by the invention, has the advantages that the required detection time is short, the specificity is strong and the sensitivity is high. The PCR detection primer disclosed by the invention can avoid the shortcomings of trivial operation, long time, low accuracy, low detection rate and the like of a traditional identification method.

The present invention provides a method for producing selenium-enriched combined nitrogen-fixing bacteria agent and its freeze-dry powder, liquid fertilizer and solid fertilizer. The culture medium of selenium-enriched combined nitrogen-fixing bacteria is composed of Na2SeO3, peptone, yeast, rare earth aqueous solution, NaCl, water etc. Three strains are respectively inoculated in flasks. Alcaligenes faecalis is represented by A15, nitrogen-fixing giant bacillus is represented by N13, Enterobacter Cloacae is represented by E26. Liquid bacterial fertilizer is obtained via rocking bottle enlarged cultivation, seed tank culture, liquid ferment in ferment tank, preservation and adjusting pH. If it is processed with vacuum freeze-dry, the freeze-dry powder is obtained. The solid fertilizer culture medium comprises grass charcoal, chicken dung, gypsum, rare earth, KH2PO4,Na2SeO3 5H2O. Fermented bacteria strain in ferment tank is inoculated at solid fertilizer culture medium and the final product is obtained after compost. The present inventive process is practical and suitable for industrial production.

The invention belongs to the technical fields of molecular biological techniques and clinical tests, and particularly discloses a nosocomial infection multiple causative agent parallel detection gene chip and a preparation method and application thereof. The gene chip can be used for rapidly and sensitively detecting fourteen medical treatment infection typical causative agents such as acinetobacter baumannii, enterobacter cloacae, enterococcus faecalis and the like by using one reaction, and the detection data of coinfection of yeast and bacterium which cannot be provided by a traditional amplification method is offered. Furthermore, the gene chip also comprises a methicillin resistance target spot, thus realizing further detection of staphy lococcus infection, therefore, a clinician can realize which causative agent a patient is infected in one or two hours, and realize whether the patient has drug resistance, so as to decide whether the patient needs antibiotics or which antibiotics is needed, so that the gene chip has a greater clinical guiding significance.

The invention discloses saline-alkaline tolerant enterobacter cloacae and a production method and application of a viable bacterium preparation of the saline-alkaline tolerant enterobacter cloacae. The saline-alkaline tolerant enterobacter cloacae ZTS-10 is separated from rhizosphere soil of chili peppers in saline-alkaline land, the preservation number of the bacterial strain in the China GeneralMicrobiological Culture Collection Center (CGMCC) is CGMCC No.20181, and the preservation date is 3 July, 2020. The bacterial strain is high in saline-alkaline tolerance and high in field planting rate in the saline-alkaline soil, has the capacity of producing IAA, degrading phosphorus and degrading potassium, can promote growth of the chili peppers, has good antagonism effects on pathogenic bacteria of chili pepper epidemic diseases, can be used for producing microorganism fertilizers especially microorganism fertilizers special for the chili peppers in the saline-alkaline land, can reduce the generation of chili pepper epidemic diseases, and can increase yield and quality.

The invention discloses enterobacter cloacae with the aerobic denitrification function and an application of the enterobacter cloacae in wastewater treatment. The enterobacter cloacae are enterobacter cloacae HNR and are collected to the China General Microbiological Culture Collection Center (CMGCC) on January 13th, 2015, the collecting number is CGMCC No.10348. According to the enterobacter cloacae, under the aerobic condition, NO3<->-N can be converted into a gaseous product through the aerobic denitrification effect with organic carbon as the unique carbon source and the NO3<->-N (nitrate nitrogen) as the unique nitrogen source, and synchronous removing of nitrogen elements and the organic carbon is achieved. According to the enterobacter cloacae, the removing rate for the NO3<->-N is as high as 90% to 98 %, the removing rate for the total nitrogen can be 85% to 95 %, and the removing rate for the total organic carbon can be higher than 85%. The enterobacter cloacae can be used for effectively removing the NO3<->-N in the organic wastewater, and have the effects that the whole process is aerobic, and the nitrogen removal efficiency is high.

The invention discloses a molecular beacon and a kit for quickly identifying various clinically common bacteria and belongs to the technical field of microbiological detection. The sequences SEQ ID of the molecular beacon are as shown in v6p1, v6p2, v1p1 and v1r. Through comparison between various clinically common bacteria 16s ribosome RNA sequences in an NCBI gene sequence database and design of the molecular beacon at a tag sequence, clinically common 18 bacteria, including baumanii, A.hydrophila, burkholderia cepacia, citrobacter freundii, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus mirabilis, pseudomonas aeruginosa, staphylococcus epidermidis, staphylococcus aureus, salmonella, serratia marcescens and stenotrophomonas maltophilia, can be quickly identified. The invention further discloses the kit containing the molecular beacon and used for detecting various bacteria, and the kit can quickly and accurately identify various clinically common bacteria.

The invention relates to a nucleotide sequence for coding L-threonine aldolase separated from the enterobacter cloacae. The nucleotide sequence is a nucleotide sequence shown in SEQ ID NO:1, or a fragment, an analogue and a derivative of the nucleotide sequence. The nucleotide sequence for coding L-threonine aldolase is connected with an exogenous regulation sequence; and a carrier for functional expression, a cellular organism containing the carrier and a descendant of the organism are contained. A method for preparing the L-beta-hydroxy alpha-amino acid or D-beta-hydroxy alpha-amino acid from the nucleotide sequence, or polypeptide sequence, or cellular organism containing the carrier and descendant of the organism is provided.

The invention relates to the technical field of microorganisms, in particular to streptococcus symcsis 3-17 streptococcus and an application thereof. Experiment proves that the streptococcus providedby the invention is named as streptococcus symcsis sp.nov.3-17, and is registered and preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC 60684; and the streptococcus has broad spectrum resistance and long-term antagonizing stability, and can resist one or more of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, proteus vulgaris, enterobacter cloacae and acinetobacter baumannii. The streptococcus GDMCC 6068 provided by the invention can be applied as probiotics, corresponding products capable of being practically applied can be prepared from the streptococcus GDMCC 6068, and the streptococcus GDMCC 6068 has quite application tremendous potential in the field of medicine and pharmacy.

The invention relates to enterobacter cloacae which are found in soil and have the collection number of CGMCC No.2487 or resting cell with equivalent function, variant and mutant as well as enzyme produced thereby. The invention also provides a preparation method of taxane, namely comprising the following steps: the enterobacter cloacae are used for hydrolyzing 7-xylose group in 7-xylose taxane molecule in wheat bran culture medium, and 13-side chain in taxane molecule with 13-side chain can be hydrolyzed in peptone beef extract culture medium; and the produced taxane can be used for preparing antitumor drugs, such as intermediates of taxane drugs, docetaxel drugs or taxane drugs.

The invention relates to a compound bacterium colony for de-coloring papermaking industrial wastewater and a preparation method thereof. The compound bacterium colony is prepared from 2 to 10 parts of amycolata bacillus, 10 to 20 parts of curtobacterium flaccumfaciens, 20 to 30 parts of white rot fungi, 10 to 15 parts of pseudomonas putida, 20 to 30 parts of agrobacterium, 10 to 30 parts of pseudomonas stutzeri and 20 to 30 parts of enterobacter cloacae. According to the compound bacterium colon provided by the invention, microorganisms have a beneficial mutual effect and the microorganisms are mixed, cultured and domesticated; certain improvement is carried out on a flowing direction of intermediate metabolites of pollutants by bacteria and inhibitive intermediate metabolites are not generated or are transformed as soon as possible, so that the degradation efficiency of the pollutants is improved.

The invention provides a kit for the detection of Enterobacter cloacae, including guide RNAs specifically targeting the rpoB gene of the Enterobacter cloacae, a primer pair for amplification, hydrated TwistAmp basic kit reaction drying balls, LbCas12a proteins, a single-stranded DNA probe (ssDNA), Ribonuclease Inhibitor and buffer. The kit can be used to detect the Enterobacter cloacae with high specificity, and can distinguish the Enterobacter cloacae from other Enterobacter bacteria, including Enterobacter aerogenes, Enterobacter gergoviae and Enterobacter sakazakii. At the same time, the method using the RNA sequence described in the invention for detection takes about 1 hour, does not need any PCR instruments, and has low requirements for equipment; and time consumption of the method is significantly lower than that of the conventional bacterial culture method or the PCR sequencing method, with great clinical practical value.

The invention discloses intestinal microorganism markers for detecting multiple myeloma diseases, and an application and detection preparation of the intestinal microorganism markers. The intestinal microorganism markers comprise citrobacter freundii, enterobacter cloacae, raoultella ornithinolytica, klebsiella aerogenes, klebsiella variicola, klebsiella pneumoniae, streptococcus salivarius, streptococcus oralis, streptococcus gordonii, streptococcus mitis, streptococcus pneumoniae, butyrate producing bacteria, clostridium butyricum and clostridium saccharobutylicum. The intestinal microbial markers can be used alone or in combination. The invention discloses a support vector machine (SVM) classification model constructed by taking the microbial markers as characteristics, and the SVM classification model is applied to clinical diagnosis of multiple myeloma diseases. The invention also discloses an application of the microorganism markers in preparation of a diagnostic preparation formultiple myeloma diseases.

The invention discloses Enterobacter cloacae ZJPH1903 and application thereof in preparation of (R)-2-chloro-1-(2, 4-dichlorophenyl)ethanol. A method of catalytic preparation of optically pure (R)-2-chloro-1-(2, 4-dichlorophenyl)ethanol with the new strain has the advantages of good stereoselectivity, high product optical purity and the like, and when the substrate concentration is 4g/L, the e.e.Value of the target product (R)-2-chloro-1-(2, 4-dichlorophenyl)ethanol is greater than 99.9%, and the yield is 92.9%. When a surfactant is added into the reaction system, the catalytic efficiency canbe effectively improved, the concentration of the catalytic substrate can be increased to 10g/L, the yield of the target product (R)-2-chloro-1-(2, 4-dichlorophenyl)ethanol reaches 94.3%, and the e.e. Value is greater than 99.9%.

The invention provides a reagent kit for detecting enterobacter aerogenes. The reagent kit contains a guide RNA specifically targeted to an enterobacter aerogenes rpoB gene, an amplification primer pair, a hydration TwistAmp basic kit reaction drying ball, LbCas12a protein, a single-stranded DNA probe (ssDNA), a Ribonuclease inhibitor and a buffer solution. When the reagent kit is used for detecting the enterobacter aerogenes, the detection specificity is high, and the enterobacter aerogenes can be distinguished from other enterobacter bacteria including enterobacter cloacae, enterobacter gergoviae and enterobacter sakazakii. Besides, when the RNA sequence is used for detection, time consumption is about 1 hour, a PCR instrument is not needed, requirements for equipment are low and are notably lower than those by a traditional bacteria culture method or a PCR sequencing method, and the clinical use value is high.

The invention relates to an endophytic fungi strain and a metabolite extraction method and a purpose thereof, which belong to the field of microbe and microbe medicines. Through morphological characteristic observation and its rDNA ITS sequence analysis and identification, the strain is Fusarium sp, and has obvious bacteriostatic activity. A metabolite extract product of the strain has obvious bacteriostatic activity for wild bacterial strains such as escherichia coli, staphylococcus aureus, bacillus subtilis as well as drug-resistant strains such as beta-lactamase gene recombinant plasmid-carried escherichia coli or klebsiella pneumonia, and has obvious bacteriostatic activity for clinically separated superbacteria simultaneously carrying with a plurality of beta-lactamase gene escherichia coli, enterobacter cloacae and klebsiella pneumonia as well as methicillin-resistant staphylococcus aureus.

The invention discloses an antibacterial amidine oligomer with drug resistance, and a preparation method and an application thereof. The antibacterial amidine oligomer comprises the following antibacterial types: Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter cloacae. The structure disclosed by the invention has dual antibacterial mechanisms of destroying cell membranes and combining chromosome DNA, has the characteristics of rapid sterilization and drug resistance, and shows antibacterial broad spectrum. In addition, according to the structure, the difference that bacteria have no cell nucleus but have cell nucleus is utilized, DNA of the bacteria is specifically targeted, the cytotoxicity of the bacteria is remarkably reduced, and the treatment index of the bacteria is improved.

The invention belongs to the field of quality control in microbial detection and particularly relates to a standard sample of salmonella in milk powder and a preparation method of the standard sample of the salmonella in the milk powder. The standard sample of the salmonella in the milk powder comprises a target bacterium and background flora, wherein the target bacterium is the salmonella; the background flora comprises Bacillus cereus, Staphylococcus aureus, Enterobacter cloacae and Enterobacter aerogenes. The standard sample meets requirements for uniformity and stability, the stability of the standard sample can be guaranteed to the greatest extent, and a perfect preparation technology for the standard sample is formed by starting from meeting special transport conditions by means of research in special process, stability and uniformity and the like, is suitable for preparing the standard sample, meeting the requirements for uniformity and stability, of the salmonella in the milk powder and is applicable to quality control, method validation and other purposes in a laboratory.

The invention discloses a LAMP primer group for detecting enterobacter cloacae, a kit and a rapid detection method. The LAMP primers are six specific primers designed according to a dnaJ gene of enterobacter cloacae. By applying the six primers, specific amplification can be realized, the enterobacter cloacae can be effectively identified, and the lowest detection limit can reach 1 DNA copy; a DNA sample can be detected within 60min; and the detection method can be flexibly selected according to actual conditions, and the primer group is convenient and rapid and has a very wide application prospect.

The invention belongs to the field of quality control in the aspect of microbiological test, and particularly relates to a standard sample for total plate count in milk powder and a preparation method thereof. A target flora of the standard sample for total plate count in milk powder is composed of bacillus cereus, escherichia coli, klebsiella pneumonia, enterobacter cloacae and staphylococcus aureus. The uniformity and stability of the standard sample for total plate count in milk powder meet the requirements on the standard sample, the stability of the test sample can be ensured to the max, and even if the content of escherichia coli is changed during transportation and storage, the total plate count is not affected in a statistical sense; and the preparation method for the sample has the advantages of simple process and high success rate.