11 results about "Carassius auratus" patented technology

The application discloses a method for creating a new strain of Carassius auratus gibelii with resistance to Cyprinid herpesvirus 2 (CyHV2) infection, and the method is characterized in that a gene editing technology is used to target knockout gsdf-a a gene and gsdf-b a gene, and an experimental animal infection model of a homozygous knockout strain of the gene obtained by the technology is established. gsdf Results of the experimental animal infection model show that gsdf the CyHV2 infection resistance of the CyHV2 gene knockout Carassius auratus gibelii is significantly enhanced, the histopathological damage of the Carassius auratus gibelii after being infected with the virus is reduced, the expression of a virus protein ORF47 in liver tissue of the Carassius auratus gibelii is reduced, and the transcription level of a virus gene orf46r is significantly reduced. The new strain of Carassius auratus gibelii created by the method can avoid exogenous gene pollution, and has the advantages that the CyHV2 infection resistance of the Carassius auratus gibelii is significantly enhanced.

The present application relates to the technical field of feeding of crucian carp, and discloses a compound functional feed for repairing carbonate alkali damage of crucian carp, which is composed of alpha-ketoglutaric acid, linoleic acid, laurylene and basic feed, wherein the weight ratio of the alpha-ketoglutaric acid accounts for 1.5% of the total weight of the compound functional feed for repairing carbonate alkali damage of crucian carp, and the weight ratio of the linoleic acid accounts for 0.7% of the total weight of the compound functional feed for repairing carbonate alkali damage of crucian carp. The compound functional feed for repairing carbonate alkali damage of crucian carp can significantly enhance the repair capacity of the tissue cells of the crucian carp, improve the disease resistance of the crucian carp, reduce the risk of diseases and promote the energy metabolism process of the crucian carp, so as to ensure the efficient use of energy and maintain the normal homeostasis of the body.

The invention discloses a method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms and application thereof, and the method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms comprises the following steps: (1) collecting and diluting white crucian carp sperms, and then carrying out inactivation treatment by ultraviolet lamp irradiation; (2) mixing the inactivated seminal fluid of the carassius auratus and the inactivated mature eggs of the sinilabeo nolatus, performing dry insemination, then pouring the mixture into cold water for treatment, putting fertilized eggs into a normal-temperature water incubation tank for incubation after cold treatment is finished, and keeping running water to enable the fertilized eggs to float along with water flow and not to sink to the bottom; and (3) after fry are hatched from the fertilized eggs, culturing to obtain gynogenetic sinilabeo nolabeo. According to the method, the carassius auratus sperms are adopted to induce the gynogenesis of the semilabeo nolabeo to successfully breed and obtain the gynogenesis semilabeo nolabeo offspring, which is the first time in the artificial induction gynogenesis and germplasm improvement of the semilabeo nolabeo. In the breeding process, it is found that the 6-month-old semilabeo notabilis has the advantages that the growth speed is high, and the low-temperature resistance is remarkably higher than that of the parent semilabeo notabilis.

The present application belongs to the field of fish breeding, and discloses a method for simultaneously breeding natural gynogenesis of Procyprinus and hybrid offspring of Cyprinus carpio, comprising the following steps: taking Procyprinus as the female parent and taking red crucian carp as the male parent to perform special pond breeding, after artificial induction of parturition, performing artificial insemination after the parent fish is in estrus, then performing flow water hatching on the fertilized eggs, after the fry is hatched, performing feeding, and then performing detection and screening on the fed fish, thereby obtaining the natural gynogenesis of Procyprinus and the hybrid offspring of Cyprinus carpio. The present application changes the activity of the ovum and sperm of the parent fish by performing distant hybridization of Procyprinus and red crucian carp and combining with specific types and dosages of parturition inducers, and thereby obtains the natural gynogenesis of Procyprinus and the hybrid offspring of Cyprinus carpio which are both sexually fertile.

The invention provides a myxocyprinus asiaticus individual genetic resurgence method which comprises the following steps: S1, taking a myxocyprinus asiaticus gonad tissue, cleaning, cutting into pieces, adding an L-15 culture medium containing double antibodies and a DPBS buffer solution, and centrifuging to obtain the gonad tissue; s2, performing water bath on the gonad tissues to obtain resuscitated gonad tissues; s3, digesting, filtering and centrifugally purifying the resuscitated gonad tissues to obtain germline stem cells; s4, injecting morpholino to obtain gobiocypris rarus without primordial germ cells; and S5, transplanting the germline stem cells into gobiocypris rarus juvenile fish bodies, and culturing to obtain the myxocyprinus asiaticus gametes. S6, the generated myxocyprinus asiaticus gametes are fertilized, and myxocyprinus asiaticus individuals are obtained. According to the method, the sperms of the myxocyprinus asiaticus are successfully obtained by utilizing a gonad cryopreservation and abdomen borrowing reproduction technology, and a feasible way is provided for individual genetic resurgence of rare and endangered fishes.

The application discloses a method for fine positioning of anti-sporozoan genes of Carassius auratus based on linkage disequilibrium analysis, relates to the technical field of fine positioning of fish genes, and comprises the following steps: collecting whole blood samples of a Carassius auratus population, extracting genomic nucleic acid sequences, constructing a whole genome marker site set with a preset marker density gradient, dividing haplotype blocks to generate an initial haplotype data set, obtaining a population linkage disequilibrium distribution map through sliding window comparison, selecting a region with a decay rate lower than a standard value as a candidate correlation section and extracting a genotype coding sequence, matching and calculating correlation strength values with sporozoan infection survival phenotype data, reconstructing haplotype phase information of the candidate section and iteratively calculating until the threshold is met if the preset threshold is not reached. The method is suitable for genetic structure differences of genomes, optimizes correlation determination processes, and improves the accuracy of anti-sporozoan gene positioning and the reliability of section screening.

The invention discloses a Chuzhou crucian carp anti-sporozoan gene fine localization method based on linkage disequilibrium analysis, and relates to the technical field of fish gene fine localization, the Chuzhou crucian carp anti-sporozoan gene fine localization method comprises the following steps: collecting Chuzhou crucian carp population whole blood samples, extracting genome nucleic acid sequences, constructing a whole genome marker site set according to a preset marker density gradient, dividing haplotype blocks to generate an initial haplotype data set, comparing through a sliding window to obtain a population linkage imbalance distribution map, selecting an area of which the attenuation rate is lower than a standard value as a candidate association section, extracting a genotype coding sequence, matching with sporozoan infection survival phenotype data, and calculating an association strength value; and if the preset threshold value is not reached, reconstructing haplotype phase information of the candidate section and carrying out iterative calculation until the threshold value is met. The method adapts to the genetic structure difference of the genome, optimizes the correlation judgment process, and improves the accuracy of anti-sporozoan gene localization and the reliability of section screening.

The application provides a method for genetic reactivation of individual Procyprinus palpebrosus, comprising the following steps: S1, taking the gonadal tissue of Procyprinus palpebrosus, washing, cutting, adding L-15 culture medium containing double antibodies and DPBS buffer solution to centrifuge the gonadal tissue; S2, water-bathing the gonadal tissue to obtain recovered gonadal tissue; S3, digesting the recovered gonadal tissue, filtering, centrifuging and purifying to obtain germ stem cells; S4, injecting morpholino to obtain rare gobi-ocypris rarus with deleted primordial germ cells; S5, transplanting the germ stem cells into the body of the rare gobi-ocypris rarus juvenile to culture, and obtaining the gametes of Procyprinus palpebrosus; and S6, fertilizing the generated gametes of Procyprinus palpebrosus to obtain the individual Procyprinus palpebrosus. The application successfully obtains the sperm of Procyprinus palpebrosus by using the cryopreserved gonadal tissue and the technology of parthenogenesis, and provides a feasible way for the individual genetic reactivation of rare and endangered fish species.

The invention discloses a crucian circular RNA (Ribonucleic Acid), an overexpression vector thereof, a preparation method of the overexpression vector and application of the overexpression vector and the overexpression vector. The nucleotide sequence of the crucian circular RNA is shown as SEQ ID NO.1. According to the application, the function of circdmrt1 in testis development of red crucian carp, white crucian carp and filial generation carassius auratus and carassius auratus No.2 is completely elaborated for the first time, it is clear that circdmrt1 can inhibit cell apoptosis and promote cell proliferation to participate in testis development, a circdmrt1 overexpression vector is provided, and a foundation is laid for application of a circdmrt1 preparation in aquatic product breeding in the future.

The invention relates to the technical field of aquatic product breeding and gene engineering, in particular to a rapid all-male red crucian carp creation method and application thereof.The rapid all-male red crucian carp creation method comprises the steps that a CRISPR / Cas9 gene editing technology is utilized, a Foxl3 gene of wild type red crucian carp is knocked out in a targeted mode through double gRNA loci, an F0-generation gene knockout mutant is obtained, and the double gRNA loci are located on a third exon of the Foxl3 gene; and hybridizing the female fish in the F0-generation gene knockout mutant with the wild-type red crucian carp male fish to obtain the F1-generation all-male red crucian carp with the genotype of Foxl3 + / -. According to the method, complete separation and stable inheritance of phenotypes can be realized in the F1 generation.