5 results about "Cyprinus" patented technology

This invention discloses a precise and efficient breeding method for *Cyprinus dorsiflorus* based on habitat reconstruction. The breeding pond is divided into a parent mussel rearing area and a glochidia larva metamorphosis development area by a barrier wall consisting of an upper, switchable isolation net and a lower, fixed partition. The glochidia larva metamorphosis development area is constructed with a 20-mesh movable net forming two layers. After laying the substrate, mature parent mussels and host fish are introduced. The temperature is controlled at 22.0–24.0℃, and fertilization is promoted with a water flow of 0.08–0.15 m / s, while glochidia larvae are cultivated with a water flow of 0.06–0.10 m / s. Once the glochidia larvae in the brood pouch mature, the isolation net is opened, and the water flow is adjusted to 0.02–0.05 m / s to promote parasitism. Every 6–8 days, the parasitized and molted parent mussels and host fish are circulated and exchanged. After the molted mussels molt, they are threaded through the net and placed into the lower substrate for in-situ cultivation. This method achieves in-situ integration of fertilization, parasitism, and cultivation, significantly improving the parasitism rate and the survival rate of juvenile mussels. It is suitable for the preservation, propagation, and large-scale seedling cultivation of *Cyprinus cuspidatum*.

The invention relates to the technical field of cyclina sinensis sperm preservation, in particular to a cyclina sinensis sperm freezing preservation method. The cyclina sinensis sperm freezing preservation method comprises the following steps: (1) putting parent shellfish into a seawater culture pond, and accelerating ripening for 7-10 days by using a ripening agent; the ripening agent is prepared from a spirulina extract, a radix paeoniae alba extract, a codonopsis pilosula extract, astaxanthin and alginic acid; (2) controlling 0.05-0.15 m / s water flow to scour and stimulate the cyclina sinensis, selecting individuals with sperms discharged after the parent shellfish gametes start to be discharged, and collecting the sperms of the cyclina sinensis; and (3) mixing the cyclina sinensis sperms with a cryoprotectant, pre-cooling, and then cryopreserving for a long time. Research results show that the use of the cryoprotectant can significantly reduce the degree of DNA damage, and the protection effect may be realized through a plurality of mechanisms such as direct combination of trehalose with DNA molecules, free radical removal, maintenance of the stability of a chromatin structure and the like.

The invention relates to the technical field of identification of varieties of carps, in particular to an identification method for distinguishing jian carps 2 and Fujian carps 2. The identification method provided by the invention is based on a specific sequence of the Fujian carp 2 relative to the Fujian carp 2, and in specific implementation, the specific sequences of the Fujian carp 2 and the Fujian carp 2 can be amplified by utilizing a specific primer through PCR (Polymerase Chain Reaction), so that the Fujian carp 2 and the Fujian carp 2 are distinguished. The identification method based on the specific sequence is simple in process, easier to grasp and high in accuracy, is not influenced by the growth stage of fishes, and can also be used for distinguishing jian carp 2 and Furai carp 2 even in the juvenile fish stage.

The invention belongs to the technical field of molecular marker-assisted breeding, and particularly discloses a structural variation molecular marker related to body surface black plaques of Cyprinus carpio var. Color carpio, a specific primer and application of the molecular marker in genetic breeding of Cyprinus carpio var. Color carpio. The structural variation molecular marker is located in the fourth intron region (the specific position is the 305bp position of the downstream of the fourth exon) of the Rptp gene of the color cyprinus carpio var.color homozygous black spot strain, and 357bp sequence deletion exists in DNA double chains at the position. Compared with the heterozygous black-spot strain, the heterozygous black-spot strain has one chain 357bp deletion, and the black-spot-free strain Cyprinus carpio var. Color carp does not have 357bp sequence deletion in the region. The specific primer is used for detecting the structural variation molecular marker of the Rptp gene in a black spot line color carp sample to be detected. According to the molecular marker and the specific primer, the black heterozygotes of the Cyprinus carpio var. Color can be efficiently and accurately removed, breed conservation and breeding of the black spot line Cyprinus carpio var. Color at low cost and high accuracy can be realized, and the molecular marker and the specific primer have remarkable application value.

This invention discloses a method for regulating the water temperature in the culture of *Cetus simonii*, relating to the field of fish farming technology. The method includes dividing the culture pond into an exchange zone and a flow zone; collecting first temperature information from the exchange zone and second temperature information from the flow zone; and obtaining the fish's location and activity information. The method also involves acquiring the air temperature and adjusting the inlet water temperature and inlet flow rate based on the air temperature, the first temperature information, and the second temperature information, so that the temperatures of the exchange zone and the flow zone are within a preset temperature regulation range. This method achieves water temperature regulation based on the activity information of the *Cetus simonii*, while simultaneously adjusting the inlet flow rate based on the location information of the *Cetus simonii*, preventing cold or hot water from directly and rapidly impacting the *Cetus simonii* near the inlet, thus preventing the fluctuating water temperature from affecting their health.