133 results about "Cyprinus" patented technology

The present invention discloses a cyprinus carpio feed and a preparation method thereof, belonging to the technical field of animal feed. The cyprinus carpio feed comprise the following raw materials in parts by weight: corn gluten meal 1-5 parts, sweet potato powder 2-6 parts, fish meal 5-10 parts, cottonseed meal 1-5 parts, monocalcium phosphate 1-2 parts, bone meal 1-3 parts, bentonite 1-3 parts, rapeseed meal 5-10 parts, soybean meal 1-5 parts, wheat bran 1-5 parts, rice bran 1-5 parts, duck meat powder 2-6 parts, chicken intestine powder 1-3 parts, lysine 2-6 parts, compound vitamins 1-3 parts, and methionine 1-3 parts. The cyprinus carpio feed is free of antibiotics and hormones, can not only meet a large amount of nutrients for the growth of cyprinus carpio, but also enable low incidence of the disease, is good in feeding attraction, can significantly increase the growth rate of cyprinus carpio and improve the feed conversion rate, and at the same time, is simple preparation method, low in cost, and suitable for large-scale production.

The invention relates to application of fibrauretine in a cyprinus carpio compound feed and a formula of the cyprinus carpio compound feed containing fibrauretine and belongs to the technical field of aquiculture. Fibrauretine is added to the cyprinus carpio compound feed to enhance the anti-stress and growth promotion effects. The cyprinus carpio compound feed containing fibrauretine comprises the following raw materials in percentage by weight: 8-10% of fish meal, 22-26% of soybean meal, 12-16% of cottonseed meal, 8-10% of coleseed meal, 22-25% of wheat middling, 8-10% of rice bran, 3-5% of soybean oil, 0.5-1.0% of soya lecithin, 0.01-0.04% of fibrauretine, 0.7-1.5% of calcium biphosphate, 0.05-0.1% of vitamin C organic phosphate, 0.1-0.3% of choline chloride, 0.2-0.3% of methionine, 0.4-0.5% of lysine, 0.01-0.03% of ethoxy quinoline, 0.5-0.8% of a mineral premix and 0.4-0.7% of a vitamin premix. According to the invention, fibrauretine is fully utilized to serve as a cyprinus carpio immunopotentiator, so that the use of medicines such as antibiotics is reduced, the quality safety of aquatic products is guaranteed, the anti-stress capability of cyprinus carpio is improved, the disease resistance is enhanced, the growth of the cyprinus carpio is promoted and the cyprinus carpio cultivation benefit is increased.

The invention discloses a method for breeding high-yield pond snakeheads. The method comprises the first step of pond selecting, the second step of pond cleaning and disinfecting, the third step of fingerling stocking, the fourth step of bait throwing and feeding and the fifth step of daily management. The fourth step specifically comprises the processes that a fresh bait and formula fodder matching throwing and feeding method is adopted, the fresh bait comprises small trashes, shrimps and earthworms, and the formula fodder comprises, by weight, 55 percent of imported whitefish powder, 12 percent of silkworm chrysalis, 11 percent of bone flour, 18 percent of rice bran, 2 percent of yeast, 1 percent of lecithin, 0.5 percent of vitamin and 0.5 percent of mineral substance. According to the method, the method of breeding the snakeheads and a small number of carps in a mixing mode is adopted, the throwing and feeding method of matching the formula fodder with the high-protein content and high absorbing and digesting ratio with the fresh bait is combined, the snakeheads can grow quickly and are strong in immunity, the per mu yield reaches to 3000 kg after six-month breeding period, and economical benefits are obviously improved.

A method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala includes the following steps that firstly, female cyprinus carpio and male megalobrama amblycephala which have obvious sexual maturity characteristics and good somatic features are selected as parent fishes for special pool breeding, then, artificial spawning induction is conducted on the parent finishes in a breeding season, the parent fishes with a good spawning induction effect are selected to be subjected to artificial dry-method fertilization, running water incubation is conducted on fertilized embryos in a pure water incubation device, fish fry are raised after being incubated, the raised fish fry are detected and screened, hybrid offspring of the cyprinus carpio and the megalobrama amblycephala are obtained through flow cytometry DNA content determination and renal tissue chromosome ploidy detection, and the hybrid offspring comprise diploid natural gynogenesis cyprinus carpio, diploid natural gynogenesis mirror carps, diploid fish like the cyprinus carpio and tetraploid hybrid fish. The method has the advantages that the rate of fertilization is high, the incubating rate is high, the offspring are excellent in character, and the method has great significance in genetic breeding.

The invention belongs to an aquatically produced animal feed and particularly relates to a mixed feed for a health-care Yellow River carp. The mixed feed contains plant ethereal oil, wherein the mass content of cinnamic aldehyde and carvacrol in the plant ethereal oil are equal to or greater than 25 percent. According to the mixed feed for the health-care Yellow River carp provided by the invention, various nutritional needs for Yellow River carp are met; the survival rate and disease resistance of the Yellow River carp are improved; the mixed feed has a favorable growth promoting function and contains no antibiotic; potential risks for human health due to residual antibiotics in fish bodies can be avoided; the requirement for health-care products is satisfied; the commercial value of the Yellow River carp is improved.

The invention relates to a screening method of dominant hybridization species of German mirror carps and discloses a screening method of the dominant hybridization species of carps. The invention provides an effective screening method of the dominant hybridization species of the German mirror carps so as to ensure the genetic diversity of hybrids thereof, thus reducing the workloads, shortening the breeding period, decreasing the breeding cost and improving the success rate of selective breeding. The screening method includes the following steps: 1. morphological marker; 2. microsatellite molecular maker; and 3. data analysis. The method screens the dominant hybridization species of the German mirror carps by combining the morphological marker and the microsatellite molecular marker. The genetic differences between the species are dually tested and the screening results are more accurate.

The invention discloses a cross breeding method for Japanese crucian carp and cyprinus carpio red. As proved by experiments, the combination of the Japanese crucian carp (female) and the cyprinus carpio red (male) which are hybridized has high affinity, and a hybridized generation F1 with superior quality can be obtained. The hybridized generation F1 has the excellent characteristics of infertility, omnivory, high growing speed, large size, good meat quality, good taste, high disease resistance and the like, is capable of overcoming certain biological defects of the Japanese crucian carp and the cyprinus carpio red effectively, and has remarkable hybridizing advantages, and the germplasm is difficult to degenerate.

The invention discloses a composite immunity reinforcing agent for cyprinus carpio var. koi. The reinforcing agent comprises the following components in percentage by mass: 35 to 45 percent of raffinose, 25 to 35 percent of low molecular chitosan oligosaccharide, 15 to 20 percent of glycyrrhetic acid and 10 to 15 percent of lactic acid bacillus. The reinforcing agent can obviously reinforce the immunity and the disease resistance of the cyprinus carpio var. koi and improve the survival rate by 12 to 19 percent; the immune effect of the composite immunity reinforcing agent is remarkably higher than that of a single immunity reinforcing agent, and the composite immunity reinforcing agent also can overcome immune suppression caused by long-term use of a single product; and a method for preparing the composite immunity reinforcing agent has the advantages of stable and low-cost raw material source and simple production process.

The invention relates to a regulation and control method for improving the breeding efficiency of Songpu cyprinus carpio. The method is used for solving the technical problems that the breeding efficiency of an existing method for breeding Songpu cyprinus carpio is low, a breeding water body is seriously polluted, and the survival rate of carps is low. The method comprises the following steps that a microporous oxygenation device is placed in a pond, disinfection is carried out, and after water is added, the microporous oxygenation device is started for continuous bottom stirring; after fishes are placed in the pond for 7-10 days, EM bacteria and a water quality regulating agent are added at the same time; when the total ammonia nitrogen of the water body of the pond reaches 1.0 mg/L, the water quality regulating agent is added again. On the basis that the breeding water body is not replaced, mass propagation of beneficial microorganisms in the water body of the pond is promoted by manually stirring bottom sludge of the pond before the fishes are placed into the pond, controlling oxygenation and adding carbohydrate after the fishes are placed into the pond, the water quality environment of the breeding pond is effectively improved, no antibiotic medicines are used, and the aims of energy conservation, emission reduction and healthy and efficient breeding are achieved. The invention belongs to the regulation and control field for improving the fish breeding efficiency.

The invention discloses carp overwintering feed. The following raw materials by weight percent are added into carp feed one month before the overwintering: 1 to 30 percent of wheat, 0.01 to 0.50 percent of vitamin C, 0.001 to 0.03 percent of vitamin E and 0.001 to 0.02 percent of vitamin K3. The carp overwintering feed is safe and high-efficient, is simple and convenient to operate and has strongfeasibility. In addition, a preparation method of the carp overwintering feed is simple and feasible, so favorable social value can be created.

The invention discloses a culter alburnus gynogenesis inducing method. The culter alburnus gynogenesis inducing method comprises the following steps that (1) cyprinoid seminal fluid is collected; (2) ultraviolet inheritance inactivation processing is carried out on the cyprinoid seminal fluid; (3) mature eggs of culter alburnus are collected, and dry insemination is carried out on the cyprinoid seminal fluid after ultraviolet inheritance inactivation processing; (4) cold shock processing is carried out on fertilized eggs, then the fertilized eggs are put into an incubation container to be incubated, and normally alive fries become gynogenesis fish. According to the culter alburnus gynogenesis inducing method, ultraviolet inheritance inactivation sperms of common cyprinoid are adopted to stimulate the culter alburnus eggs, release of second polar bodies of the eggs is restrained through cold shock to promote chromosome doubling, and culter alburnus individuals are successfully obtained. Normally alive individuals are the gynogenesis fish, subsequent identification analyses of chromosomes, genetic markers and the like are not needed, and identification procedures are significantly simplified.

The invention discloses a method for extracting and cultivating primary cyprinidae hepatocytes, and is applicable to the field of bioengineering.The method includes carrying out dissection on selected cyprinidae, fetching and shearing livers under sterile conditions, digesting the livers by the aid of trypsin, then filtering the livers by the aid of hepatocytes strainers to obtain hepatocytes suspension, purifying the hepatocytes suspension by the aid of Perco111 and adding cultivation liquid with cyprinidae serum into the hepatocytes suspension to obtain new hepatocytes suspension; counting small blood spheres by the aid of small blood sphere counting plates, cultivating and proliferating the primary cyprinidae hepatocytes by the aid of plate laying processes under the most suitable cultivation conditions and carrying out microscopy identification on the obtained primary cyprinidae hepatocytes by the aid of diversified processes.The method for purifying and cultivating the primary cyprinidae hepatocytes has the advantages that the livers of the cyprinidae are directly fetched under the sterile conditions, hepatocytes are purified by the aid of the Perco111, and the large quantities of high-purity in-vitro primary cyprinidae hepatocytes can be truly obtained via identification; matched bovine serum in the traditional cultivation media is replaced by the cyprinidae serum, accordingly, the viability of the in-vitro primary cyprinidae hepatocytes can be improved, and foundation can be laid for further carrying out toxicology tests and other experiments on the primary cyprinidae hepatocytes.

The invention discloses traditional Chinese medicine carp feed for improving immunity. The traditional Chinese medicine carp feed is made of, by weight ratio, 40-60 parts of bean cakes, 10-20 parts of fish meal, 15-25 parts of rice bran, 5-8 parts bighead atractylodes rhizome, 7-10 parts of liquorice, 4-6 parts of astragalus membranaceus, 2-5 parts of placenta hominis, 3-6 parts of radix puerariae, 5-8 parts of suberect spatholobus stems, 2-5 parts of vitamin premix, 1-3 parts of salt, 1-2 parts of penicillin and 2-4 parts of binding agents. According to the traditional Chinese medicine carp feed for improving immunity, the adopted bean cakes, fish meal and rice bran can meet the normal nutritional requirement of carp, the rice bran and the salt can supplement the necessary microelement in the carp bodies, the immunity of the carp can be enhanced by the penicillin, and the bighead atractylodes rhizome, the liquorice, the astragalus membranaceus, the placenta hominis, the radix puerariae and the suberect spatholobus stems are common medicine for improving the immunity and can improve the resistance of the carp after being mixed into the feed.

The present invention discloses a fish feed additive. The fish feed additive comprises the following components inparts by weight: 10-15 parts of semen lablab album, 3-15 parts of dried tangerine peels, 3-12 parts of fruits of russianolive, 6-15 parts of flos celosiae cristatae, 5-10 parts of radix aconiti carmichaeli, 6-15 parts of radix codonopsis, 6-15 parts of rhizoma atractylodis macrocephalae, 5-12 parts of dried gingers and 9-18 parts of prepared radix licorice. The fish feed additive can enhance the body constitution of cyprinus carpio, reduce the disease occurrence, improve the disease resistance of the fish schools on the whole, reduce the occurrence of climate stress responses, significantly reduce the incidence of diseases such as enteritis, saprolegniasis, bleeding diseases, spring viraemia, etc. of the cyprinus carpio, and ensure the breeding efficiency.

The invention relates to the technical field of aquaculture and in particular relates to a compound feed for carps. The compound feed comprises the following raw materials: wheat, bean pulp, rapeseed meal, cottonseed meal, fish powder, meat powder, a corn protein feed, corn distillers grains, sprayed blood powder, oil bran, calcium dihydrogen phosphate, compound mineral and compound vitamin. The compound feed is reasonable in formula and meets the nutritional requirements on high energy and low protein caused by an aquaculture feed. According to the technical scheme, the sprayed blood powder, the meat powder and the good-quality plant proteins are added, so that the nutritional requirements of the carps can be ensured, the usage amount of the fish powder is effectively reduced to be 2%-5% from 6%-15%, the feed cost is reduced, and the income of farmers is increased.

The present invention discloses a feed for cyprinus carpio in autumn. The feed comprises the following raw materials: 6-10 parts by weight of fish meal, 8-12 parts by weight of soybean meal, 8-12 parts by weight of rice bran, 10-13 parts by weight of rapeseed meal, 8-12 parts by weight of cottonseed meal, 10-15 parts by weight of wheat middlings, 18-22 parts by weight of wheat bran, 5-9 parts by weight of corn flour, 0.2-0.5 part by weight of mineral vitamins, 0.2-0.5 part by weight of edible salt and 1-2 parts by weight of a Chinese herbal medicinal additive. Beneficial effects are as follows: the feed enhances the body constitution of the cyprinus carpio, realizes the fattening and health caring, reduces seasonal diseases, improves the physiological functions of the cyprinus carpio and the meat quality of the cyprinus carpio, reduces feeding costs of raising households, can improve the disease resistance of the cyprinus carpio and effectively responds to the breeding in winter after the feeding of the feed.

The present invention provides the isolated causative agent of the disease affecting Cyprinus carpino and a method for its isolation. The invention further provides avirulent forms, e.g., live-attenuated form, inactivated form and genetically modified forms of the virus which may be used for vaccination of susceptible fish.

The invention relates to a molecular marker for identifying carp varieties and a method for identifying by utilizing the molecular marker, and belongs to the technical field of molecular markers. A fixed primer sequence and a random primer sequence are provided, and thenPCR reaction is carried out through the pair of primers, so as to obtain the result after electrophoresis. According to the molecular marker for identifying carp varieties and the method for identifying by utilizing the molecular marker, provided by the invention, by adopting the primer pair, a strip of obvious specific band about 200 bp is obtained only in a cyprinus carpio sample through amplification, and is not obtained in the original parent cyprinus carpiovar Jian and Yellow river carp. The molecular marker can be used for identification of carp varieties, breed conservation and breeding assisted by molecular-biological techniques.

The invention discloses an intercropping feed and intercropping method for grass carps, carps, crucian carps and variegated and white carps. The intercropping feed is prepared from the following raw materials in parts by weight: 900-950 parts of a basic coarse feed, 10 parts of soybean phospholipid oil, 10 parts of soybean oil, 3 parts of sodium chloride, 10-11 parts of zeolite powder, 20 parts ofcalcium dihydrogen phosphate, 3 parts of choline chloride, 1 or less of part of Gandankang, and 10 parts of a composite premix feed for fish. According to the intercropping feed and the intercroppingmethod disclosed by the invention, physiological habits and characteristics of the grass carps, the carps, the crucian carps and the variegated and white carps are sufficiently considered, the grasscarps are mainly raised, and the intercropping feed can concurrently raise other kinds. Primary breeding test proves that components in the feed formula can generate good synergistic effects, the fishdiseases caused by factors of nutrients, environment, bacteria and the like can be effectively prevented, and the status that when being drawn in the net, the carps bleed and the carps are not resistant to transport, can be notably improved. The intercropping feed has the advantages of improving the feed digestibility, promoting growth, reducing the feed coefficient by 0.18 or above and the like,the growth rate, the daily gain and the survival rate of various intercropped fish can be effectively increased, the whole yield can be increased, and the economic benefits can be increased.

The invention provides a simulation method for negative effects caused by controlling juvenile fish outbreak by using filter feeding organisms to control an ecological rehabilitation project. The simulation method comprises the following steps: preparing sediment, preparing lake water, adding corbicula fluminea and carps to start an experiment and sampling, wherein the density of the added corbicula fluminea and carps is corbicula fluminea density treatment (0, 60 and 180) and is converted into the unit area biomass (namely 0, 214g/m<2> and 643g/m<2>); the density of the carps is (1 and 3) and is converted into the unit area biomass (namely 4.3g/m<2> and 12.9g/m<2>). According to an experiment biological method adopted by a biological principle, the simulation of the negative effects caused by controlling the juvenile fish outbreak by using the filter feeding organisms to control the ecological rehabilitation project is accurately realized; a biological operation principle is utilized and the filter feeding organisms, namely the corbicula fluminea, are added; the sediment suspension caused by the juvenile fish outbreak of the carps is controlled by the corbicula fluminea, and the illumination conditions of a water body are improved, so that the aim of repairing the eutrophication water body is realized; the method has the characteristics of safety, low cost and the like.

The invention relates to a special feed for cyprinus carpio under alkaline water area culture conditions and a preparation method thereof and belongs to the technical field of cyprinus carpio feeds and preparation thereof. The feed is prepared from soybean meal, fish meal, middlings, bean oil, fish oil, phospholipid, choline chloride, sodium dihydrogen phosphate, mineral element premix, multivitamins, methionine, threonine, sodium butyrate, alpha-ketoglutaric acid and N-carbamylglutamic acid according to certain parts by mass. Through adding the sodium butyrate, the acid-base equilibrium of intestinal tracts can be changed, the activity of digestive enzymes is improved, the growth of probiotics is promoted to improve intestinal tract health, and growth is promoted. The alpha-ketoglutaric acid and the N-carbamylglutamic acid can convert ammonia resulting from catabolism of proteins into glutamine and arginine in a metabolism process, and nitrogen generated from metabolism is transferredinto amino acids again for reutilization, so that a toxic action on fish bodies caused by the ammonia and the nitrogen is reduced, and the utilization ratio of the nitrogen is increased; meanwhile, the generated glutamine and arginine can also be used for improving immunizing and oxidation resisting functions of organisms.

The invention provides a forage for carps. The forage comprise: 45-55 parts of bean cake, 12-18 parts of fish meal, 12-18 parts of wheat bran, 12-18 parts of rice bran, 0.5-1.5 parts of composite vitamins, 0.5-1.5 parts of inorganic salt, 0.5-1.5 parts of antibiotic leftovers and 1-3 parts of an adhesive. The forage for the carps has the following technical effects that the forage has a feed coefficient of 2.7, contains energy and protein content suitable for the carps, can balance the energy and the proteins, and has characteristics of high quality and low price.