5 results about "Gene Localization" patented technology

This invention discloses the cloning and application of the wheat broad-spectrum multi-effect disease resistance gene WAI4. This invention provides a protein, which is as follows: A1) or A2) or A3): A1) a protein comprising the amino acid sequence shown in SEQ ID No. 2; A2) a protein derived from A1) by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in SEQ ID No. 2, and having the same function; A3) a fusion protein obtained by adding a tag protein to the end of the protein shown in A1). This invention provides gene localization, map-based cloning, and biological function identification methods for the wheat stress resistance gene WAI4, demonstrating that the gene WAI4 has resistance to powdery mildew and / or stripe rust, playing an important role in improving and enhancing germplasm resources of crops such as wheat.

The application provides cloning of a soybean oil content regulating gene and its encoded protein and application, and relates to the fields of plant molecular genetics and genetic engineering. The soybean oil content regulating gene is qOil10, and the nucleotide sequence is shown in the sequence table SEQ ID NO:1. A major gene controlling soybean oil content is successfully cloned, the function of the gene in positively regulating seed oil accumulation is determined, and the shortage of insufficient soybean oil functional gene resources is filled. The application further provides gene positioning primers, knockout primers, recombination vectors and genetic transformation methods, which can be directly applied to molecular marker assisted selection breeding, significantly improve the breeding efficiency of high-oil soybeans, and shorten the breeding cycle. Compared with conventional breeding, the application is precise in trait improvement and simple in operation, and provides key technical support for cultivating new high-oil soybean varieties and ensuring oil supply safety.

This invention provides a method for preparing sweet and waxy maize with the same kernel size and its application, relating to the field of bio-breeding technology. This invention identifies and obtains for the first time a new sweet and waxy maize germplasm material, TZNB25341, formed by natural mutation. For the aforementioned new material TZNB25341, this invention conducts in-depth analysis of its key controlling genes for sweet and waxy maize with the same kernel size, and carries out systematic gene mapping and cloning studies on the genetic regulatory mechanism of this trait. It was discovered that in TZNB25341… ZmSH1 A 5979 bp retrotransposon LTR was inserted into the exon 13 region of the (Zm00001eb374090) gene. The insertion of this transposon led to three premature translation termination sequences and a significant decrease in transcription level in the exon, resulting in phenotypic changes in TZNB25341, which ultimately manifested as the sweet and sticky phenotype.

The application belongs to the technical field of genetic engineering, and relates to a kind of resistance to clubroot cabbage gene Bol.TNL.2 and its application, the nucleotide sequence of resistance to clubroot cabbage gene Bol.TNL.2 is SEQ ID No.1, and gene is located on C07 chromosome.The application is analyzed to the BC1F1 population constructed by resistance cabbage Z5 and susceptible cabbage WG12 by BSA method, target gene is located on C07 chromosome, resistance to clubroot cabbage gene Bol.TNL.2 with TNL structure is found, new clubroot resistance site is developed, and new variety of plant with broad-spectrum resistance to clubroot is bred using gene Bol.TNL.2, to meet actual demand.