60 results about "Gene Localization" patented technology

The invention relates to the technical field of crop genetic breeding and plant molecular design breeding, in particular to a pakchoi whole genome liquid phase chip and application. The pakchoi breeding gene chip disclosed by the invention comprises a target capture probe which is designed by taking 1000 core SNP (Single Nucleotide Polymorphism) molecular markers positioned on 40KSNP sites on a pakchoi reference genome version V4.0 as templates. The pakchoi whole genome liquid phase chip provided by the invention comprises a 40K site targeted capture probe mixed solution and a hybridization capture reagent. The pakchoi 40KSNP targeted capture probe can rapidly and effectively track genetic materials of pakchoi, and is suitable for different application scenes such as accurate identification of pakchoi germplasm resources, genetic relationship analysis between materials, variety authenticity identification, QTL gene positioning and mining, molecular marker-assisted breeding, variety improvement and whole genome selective breeding application. Therefore, the method has good application prospect and important social value.

The invention discloses application of wheat Rht1-D1b protein in regulation and control of a wheat tillering angle, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to increase the tillering angle of plants. Therefore, the invention provides the application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the tillering angle of the plant, wherein the protein can be Rht1-D1b protein; the Rht1-D1b protein can be a protein of which the amino acid sequence is as shown in SEQ ID No. 3. According to the application, the Rht1-D1b gene is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle of wheat. The wheat gene Rht1-D1b can be widely applied to the plant fields of wheat germplasm resource improvement, wheat plant type genetic breeding and the like, and has an important application value for improving the wheat yield.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

The invention discloses a biological material for regulating and controlling effective tillering related protein ETN1 of wheat and application of the biological material, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to improve the effective tiller number of plants and / or reduce the plant height. Therefore, the invention provides the application of the ETN1 protein or a substance for regulating the expression of an encoding gene of the ETN1 protein or a substance for regulating the activity or content of the ETN1 protein in regulating the tiller number or the plant height of the plant, and the ETN1 protein can be a protein with an amino acid sequence as shown in a sequence 2 in a sequence table. The gene of the ETN1 protein is finally localized through gene localization and map-based cloning. The gene can be widely applied to the plant fields of wheat genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and plays an important role in improving germplasm resources of crops such as wheat and the like.

The present application relates to the field of crop genetic breeding and molecular biology, and particularly relates to a highland barley beta-glucan screening index system and a major gene positioning method, which comprises the following steps: firstly, a three-dimensional coupling screening system of genetic stability, functional activity and agronomic adaptability is constructed, the index combination weight is determined by using an entropy weight method and an analytic hierarchy process, and an AMMI model is combined to correct the environmental effect and calculate the comprehensive score of the germplasm; secondly, a recombinant inbred line and a natural germplasm double positioning population is constructed, and near-infrared spectroscopy is combined with a Transformer model to quickly obtain phenotype data; thirdly, a candidate major effective segment is obtained through double population joint positioning, a major effective gene is screened out by combining a transcriptome and a metabolome co-expression network, and a functional molecular marker is developed. The present application realizes accurate evaluation of highland barley germplasm and accurate positioning of major effective genes, and the phenotype detection is efficient and lossless, the molecular marker selection is accurate, and the present application can also be applied to other cereal crops.

The present invention discloses the use of the MYC2 gene of Pansy as a positive regulatory factor in promoting the release of plant ecodormancy, relating to the field of biotechnology. The present invention, for the first time, demonstrates the important role of the MYC2 gene in releasing ecodormancy in Pansy by transiently overexpressing a homologous gene. The invention also confirms that the gene is localized in the cell nucleus and that its expression increases with the release of ecodormancy. Overexpression of the MYC2 gene in Pansy promotes the release of plant ecodormancy and germination.

The present invention relates to the gene cloning and functional application of the salt-tolerant gene Hg52609 in the roots of Halogeton glomeratus, belonging to the field of plant bioengineering technology. The salt-tolerant gene Hg52609 was cloned from the roots of Halogeton glomeratus for the first time. By cloning the salt-tolerant related gene Hg52609 in the roots of Halogeton glomeratus, a subcellular localization expression vector was constructed, and gene localization was carried out through transient expression in tobacco; an overexpression vector was constructed, and Arabidopsis thaliana was stably genetically transformed for salt tolerance identification; a yeast heterologous expression vector was constructed to further verify the function of the Hg52609 gene. It was found that the Hg52609 gene is involved in the efflux of Na+ but does not mediate the absorption of K+ under high concentrations of Na+. The present invention provides the cloning and functional application of the salt-tolerant gene Hg52609 in the roots of Halogeton glomeratus, which will be a good material for exploring plant salt-tolerant genes and studying the salt-tolerant regulation mechanism, and has important application value for improving the stress resistance of crops and cultivating salt-tolerant and alkali-tolerant crops.

The invention relates to a corn WRKY transcription factor ZmWRKY74 as well as a coding gene and application thereof, and belongs to the technical field of plant gene breeding, the CDS sequence of the coding gene of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.1, the gene negatively regulates the drought tolerance of crop plants, and the amino acid sequence of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.3. Gene positioning analysis is carried out on a drought-resistant mutant of corn, it is found that the ZmWRKY74 gene is an effect gene capable of improving the tolerance of corn to drought stress, and through a ZmWRKY74 gene overexpression experiment, the negative regulation effect of the ZmWRKY74 gene on the drought tolerance of corn plants is verified; important theoretical significance and application value are provided for creation of new corn germplasm and molecular breeding of corn.

The invention belongs to the field of crop heredity, and relates to application of a polyphenol oxidase gene. The invention discloses an InDel marker for rice brown glume traits, a regulatory gene and application of the InDel marker and the regulatory gene. Based on a brown glume mutant material in the background of Hunan early indica 42, genetic analysis shows that the mutation is dominant mutation, single-gene control and gene positioning find that the mutant gene is a polyphenol oxidase encoding gene (MSULocus is LOCOs04g53300), and the exon region of the mutant gene is deleted by 6bp (GCGACT), so that the material shows brown glume; the gene is knocked out from a brown glume mutant, and the glume color becomes normal; the method has the advantages that the GCGACTs are used as the raw materials, the GCGACTs are accurately edited in the wild type materials, the corresponding 6bp deletion (GCGACT) materials are obtained, the glumes of the GCGACTs are brown, and new gene resources and methods are provided for quickly and directionally cultivating novel rice materials and rice materials suitable for mechanized seed production of hybrid rice.

This invention provides a gene prediction and identification method, apparatus, device, and storage medium, belonging to the field of gene annotation and prediction. The method includes: acquiring the gene text to be annotated and a set of promoter-terminator pairs; preprocessing the gene text to generate a raw genome sequence; searching the raw genome sequence for base sequences that fuzzy search matches the set of promoter-terminator pairs to generate a base sequence to be aligned; and comparing the base sequence to be aligned with base sequences in a bioinformatics database based on the BLAST gene alignment method to generate gene prediction and identification results. This invention, by separating base sequences from promoter-terminator pairs and then performing BLAST gene sequence alignment, achieves coarse gene localization followed by BLAST gene sequence alignment, providing a method for predicting new genes, improving alignment efficiency and comprehensiveness, as well as the accuracy and comprehensiveness of gene prediction results.

This invention discloses the cloning and application of the wheat broad-spectrum multi-effect disease resistance gene WAI4. This invention provides a protein, which is as follows: A1) or A2) or A3): A1) a protein comprising the amino acid sequence shown in SEQ ID No. 2; A2) a protein derived from A1) by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in SEQ ID No. 2, and having the same function; A3) a fusion protein obtained by adding a tag protein to the end of the protein shown in A1). This invention provides gene localization, map-based cloning, and biological function identification methods for the wheat stress resistance gene WAI4, demonstrating that the gene WAI4 has resistance to powdery mildew and / or stripe rust, playing an important role in improving and enhancing germplasm resources of crops such as wheat.

This invention application discloses a gene regulating maize kernel development. ZmRH51 This application focuses on the encoded proteins, functional markers, and applications of maize kernel mutants. m223 Using this as the basic material, its heterozygotes were crossed with Zheng 58 (Z58) to construct the F2 segregating population. Phenotypic identification and cytological observation were performed on the progeny segregating population. Controlling populations were obtained through genetic analysis, BSR-seq analysis, and map-based cloning. m223 Genes with mutant phenotypes ZmRH51 This gene is located in the nucleolar region and encodes an RNA helicase belonging to the DEAD-Box gene family. Studies have shown that... ZmRH51 It participates in ribosome assembly and can influence the processing of pre-rRNA in maize kernels; and further mining yields... ZmRH51 The superior allelic variant genotype of this gene in terms of grain thickness can be utilized to improve grain thickness and 100-grain weight, thereby increasing yield. Furthermore, the distribution and utilization potential of different haplotypes in inbred lines were studied, providing technical support and excellent germplasm resources for the utilization of this gene.

The present invention discloses a 60K SNP liquid breeding chip for broiler chickens, which relates to the field of biological detection technology. The 60K SNP liquid breeding chip for broiler chickens includes a probe combination, which is used to identify the genotypes of 61,219 SNP loci. The functional loci and gene region loci of the 60K SNP liquid breeding chip for broiler chickens provided by the present invention are rich, the chip loci have high polymorphism and are evenly distributed on chromosomes, and it can be widely used in different application scenarios such as the construction of genetic maps of broiler chickens, gene mapping, genome-wide association analysis, molecular marker-assisted selection, directional improvement, mining and identification of genes for important traits and functional analysis, and genome-wide selection breeding for different broiler chicken samples, which is of great significance for improving the breeding efficiency of broiler chickens and promoting the high-quality development of the broiler chicken industry in China.

The invention discloses a wheat grain weight gene WGY1 as well as an encoded protein and application thereof, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to regulate and control the grain weight of wheat. To this end, the present application provides proteins and related biomaterials that modulate plant yield. The invention also provides application of the protein and related biological materials thereof in regulation and control of plant yield. According to the application, the WGY1 gene is finally obtained through gene localization and map-based cloning. The gene and the protein coded by the gene can be widely applied to the plant fields of wheat genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and play an important role in improving germplasm resources of crops such as wheat and the like.

The invention provides a gene BnaPT13 and application thereof, the nucleotide sequence of the gene BnaPT13 is shown as SEQ ID NO: 1, the gene is positioned on a cell membrane, has a transmembrane phosphorus transport effect, and can improve the utilization of rape phosphorus. The invention provides a new theoretical basis and genetic resources for cultivation of rape varieties.

The invention discloses a rice tillering angle and tillering number control gene LAZY5 and application thereof, and belongs to the technical field of biological breeding. The invention discloses a method for regulating and controlling the tillering angle and / or the tillering number of a plant, and the method comprises the step of regulating and controlling the expression quantity of a coding gene of LAZY5 protein in a receptor plant so as to regulate and control the tillering angle and / or the tillering number of the receptor plant, the LAZY5 protein is a protein of which the amino acid sequence is as shown in SEQ ID No. 2. According to the application, the coding gene of the LAZY5 protein is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle and / or the tillering number. The rice gene LAZY5 can be widely applied to the plant fields of rice genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and plays an important role in improving germplasm resources of crops such as rice and the like.

The invention relates to the technical field of gene chips, in particular to a sorghum whole genome liquid phase chip as well as a construction method and application thereof. A detection target of the sorghum whole genome liquid phase chip disclosed by the invention is composed of 5446 SNP molecular markers and 3 InDel molecular markers; the positions of the 5446 SNP molecular markers and the 3 InDel molecular markers, which are positioned on a sorghum reference genome Sorghumbicolv3.1. 1, are as shown in a table 1; the sorghum whole genome liquid chip can be used for sorghum variety identification, genetic relationship identification, genetic diversity analysis, molecular genetic map construction, gene positioning and cloning, molecular marker-assisted selection and whole genome selective breeding, and provides an important molecular basis for important agronomic trait analysis and biological breeding of sorghum.

The invention relates to a method for rapidly carrying out BSA (Bovine Serum Albumin) gene localization by utilizing a space mutation mutant. The method comprises the following steps: firstly, carrying propagules such as seeds through an aerospace craft, and constructing a broad-spectrum mutant library by utilizing space environment mutagenesis; returning to the ground, culturing and screening M1-generation mutants with target excellent characters, and hybridizing / backcrossing with a wild type to construct a character segregation population; by adopting a group separation analysis (BSA) strategy, selecting phenotype polar differentiation individuals, and respectively mixing the phenotype polar differentiation individuals to form a high-value pool and a low-value pool; and finally, carrying out whole genome high-throughput sequencing on the double pools and the parents, rapidly locking a genome region linked with a target character by combining bioinformatics analysis such as SNP-index and the like, and verifying candidate genes through gene annotation, molecular marking and gene editing. According to the invention, the broad spectrum of space mutagenesis and the high efficiency of the BSA-seq technology are integrated, the period from mutation creation to gene identification is remarkably shortened, and an innovative technical scheme is provided for crop genetic improvement and functional genomics research.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW4; the KASP marker RW4 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 537543. According to the application, the KASP molecular marker RW4 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW4 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a detection marker of a corn plant type regulatory gene and application of the detection marker. Belongs to the technical field of molecular breeding. The corn dwarf mutant d16 is subjected to phenotype identification, genetic analysis and gene positioning, and finally, the ZmD16 gene is found to be a new allele of a known gene Br2. According to the mutant, G > T single base mutation occurs at 3533bp at the downstream of a fifth exon ATG in a coding region of a wild type ZmD16 gene. A KASP molecular marker is designed according to a mutation site. A foundation is laid for deeply analyzing a molecular mechanism of a corn plant type, and a new material is provided for breeding application of density-tolerant varieties.

The invention discloses an SNP (Single Nucleotide Polymorphism) site combination for grassiness genotyping, a whole genome liquid phase chip and application of the whole genome liquid phase chip, and belongs to the technical field of gene chips. The SNP locus combination is 11319 SNP loci which are positioned on a grassiness reference genome version GCA028749085.1, and the liquid phase chip genetic typing loci comprise the SNP locus combination. The invention further discloses a detection method of the liquid phase chip genetic typing loci, and the detection method of the liquid phase chip genetic typing loci comprises the SNP locus combination and the detection method of the liquid phase chip genetic typing loci. The pennisetum purpureum whole genome liquid chip provided by the invention provides an efficient genome typing tool for genetic diversity analysis, variety identification, gene localization, background screening and molecular marker-assisted breeding of pennisetum purpureum; meanwhile, a new probe can be designed at any time according to a new site, the requirement for the sample amount is not high, different use scenes and use requirements of users can be met, and high application value is achieved in pennisetum purpureum molecular breeding.

The invention belongs to the technical field of plant genetic engineering and molecular breeding, and particularly discloses a key negative regulation gene GhHSFC1 for regulating verticillium wilt resistance of cotton and application of the key negative regulation gene GhHSFC1. The gene is positioned on a chromosome A05 of upland cotton of a genetic standard line TM-1, the coding sequence (CDS) of the gene is shown as SEQ ID NO. 1, and the sequence of a protein coded by the gene is shown as SEQ ID NO. 2. Through bioinformatics analysis and in combination with a core virus-induced gene silencing (VIGS) technology, functional verification is carried out, and it is confirmed for the first time that the GhHSFC1 gene is a negative regulatory factor of cotton verticillium wilt resistance. According to the gene resource and the application thereof provided by the invention, a novel target spot subjected to functional verification and a precise and efficient improvement strategy are provided for molecular breeding of cotton verticillium wilt resistance, and the gene resource has important research value and application prospect.

The invention discloses a brassica juncea KASP molecular marker combination and application thereof in germplasm resource identification and breeding, and belongs to the technical field of molecular markers. The mustard KASP molecular marker set is developed from 193 mustard germplasm resources based on whole genome re-sequencing, the mustard KASP molecular marker can quickly, accurately and effectively identify and evaluate germplasm resource materials or bred varieties, and the problems of resource redundancy, inconvenient storage and management, intellectual property disputes and the like caused by accumulation of a large amount of germplasm can be solved. The molecular marker is provided for genetic map construction, gene positioning and molecular assisted breeding.

The application provides cloning of a soybean oil content regulating gene and its encoded protein and application, and relates to the fields of plant molecular genetics and genetic engineering. The soybean oil content regulating gene is qOil10, and the nucleotide sequence is shown in the sequence table SEQ ID NO:1. A major gene controlling soybean oil content is successfully cloned, the function of the gene in positively regulating seed oil accumulation is determined, and the shortage of insufficient soybean oil functional gene resources is filled. The application further provides gene positioning primers, knockout primers, recombination vectors and genetic transformation methods, which can be directly applied to molecular marker assisted selection breeding, significantly improve the breeding efficiency of high-oil soybeans, and shorten the breeding cycle. Compared with conventional breeding, the application is precise in trait improvement and simple in operation, and provides key technical support for cultivating new high-oil soybean varieties and ensuring oil supply safety.

The invention provides a saline-alkaline tolerant rice genome breeding and detection chip and application thereof, the chip comprises 5450 SNP sites distributed in 12 chromosomes, 11080027 initial sites are screened according to the principle that the heterozygosis rate is less than or equal to 15% and the deletion rate is less than or equal to 10%, and the probe design meets the requirements that the length is 110nt and the GC content is 30-70%; the 5K liquid phase chip developed on the basis of a GBTS technology and a GenoBaits method can be used for saline-alkaline tolerant rice germplasm resource screening, genome selective breeding and rice variety identification, molecular marker breeding is assisted through genotype identification and detection, the problems of inaccurate saline-alkaline tolerant gene positioning and low detection efficiency in Heilongjiang traditional breeding are solved, and the application prospect is wide. The combination of traditional and modern technologies is promoted, technical support is provided for molecular breeding and seed quality monitoring of cold region rice, and the method has important significance for improving local rice quality and guaranteeing grain safety.

The invention discloses a gene for controlling existence of wheat tannin and a molecular marker and application thereof. The gene is positioned in a region of 701.8 to 704.3 Mb of a chromosome 3A of a reference genome of wheat in spring in China. According to a generic genome and gene expression result (RNA-seq), a candidate gene is locked and named as TaMYB10-3A, three primer markers are designed on the gene to form a primer combination, and the primer combination can be used for detecting whether wheat contains tannin or not, so that a new means is provided for identifying wheat tannin, and the application prospect is broad. The molecular marker primer combination can be applied to wheat tannin breeding practice in a simple, convenient, rapid and high-throughput mode, the wheat tannin character improvement process is accelerated, and the breeding accuracy and selection efficiency are improved.

The present invention relates to the technical field of molecular detection. Disclosed are a Camellia sinensis liquid-phase chip and the use thereof. Disclosed in the present invention are a Camellia sinensis liquid-phase chip and the use thereof. According to locus screening requirements and probe design principles, the chip comprises 5781 SNP loci, and can achieve the genotyping of Camellia sinensis resources on the basis of a precise positioning sequence-based typing technology via the liquid-phase capture of a genomic sequence at a target interval. The Camellia sinensis liquid-phase chip of the present invention can enable low-cost genotyping, and is mainly specific to Camellia sinensis. The chip can realize cultivar identification and genetic relationship analysis of Camellia sinensis, can provide scientific guidance for Camellia sinensis hybridization and improvement programs, and can facilitate the conservation and exploitation of Camellia sinensis germplasm resources, and thus has a high application value in multiple fields of Camellia sinensis breeding. The Camellia sinensis liquid-phase chip of the present invention can be used for genetic diversity evaluation of Camellia sinensis, germplasm resource and genetic relationship identification, genetic map construction and gene mapping, genome-wide association studies, and molecular marker-assisted breeding of Camellia sinensis.

This invention provides a method for preparing sweet and waxy maize with the same kernel size and its application, relating to the field of bio-breeding technology. This invention identifies and obtains for the first time a new sweet and waxy maize germplasm material, TZNB25341, formed by natural mutation. For the aforementioned new material TZNB25341, this invention conducts in-depth analysis of its key controlling genes for sweet and waxy maize with the same kernel size, and carries out systematic gene mapping and cloning studies on the genetic regulatory mechanism of this trait. It was discovered that in TZNB25341… ZmSH1 A 5979 bp retrotransposon LTR was inserted into the exon 13 region of the (Zm00001eb374090) gene. The insertion of this transposon led to three premature translation termination sequences and a significant decrease in transcription level in the exon, resulting in phenotypic changes in TZNB25341, which ultimately manifested as the sweet and sticky phenotype.

The invention discloses application of a KASP molecular marker in identification of red peel / yellow peel characters of muskmelon, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on the red peel / yellow peel character of the muskmelon. The invention relates to a method for identifying red peel / yellow peel characters of muskmelons, which comprises the following steps: extracting the genome DNA of a target muskmelon, carrying out PCR amplification by taking the genome DNA of the target muskmelon as a template, and carrying out fluorescence detection based on a primer pair of a KASP molecular marker RY-1 or RY-2 to obtain the gene type of the target muskmelon, on the basis of the detected gene type, judging the character of the target muskmelon; wherein the KASP molecular marker RY-1 and the KASP molecular marker RY-2 are respectively designed according to SNP (Single Nucleotide Polymorphism) loci at the position of Chr04: 469552 and the position of Chr04: 760234. The identification method provided by the invention can be more efficiently applied to the fields of muskmelon molecular marker-assisted breeding, variety purity detection, gene editing target screening and the like.