44 results about "Gene Localization" patented technology

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

The present application relates to the field of crop genetic breeding and molecular biology, and particularly relates to a highland barley beta-glucan screening index system and a major gene positioning method, which comprises the following steps: firstly, a three-dimensional coupling screening system of genetic stability, functional activity and agronomic adaptability is constructed, the index combination weight is determined by using an entropy weight method and an analytic hierarchy process, and an AMMI model is combined to correct the environmental effect and calculate the comprehensive score of the germplasm; secondly, a recombinant inbred line and a natural germplasm double positioning population is constructed, and near-infrared spectroscopy is combined with a Transformer model to quickly obtain phenotype data; thirdly, a candidate major effective segment is obtained through double population joint positioning, a major effective gene is screened out by combining a transcriptome and a metabolome co-expression network, and a functional molecular marker is developed. The present application realizes accurate evaluation of highland barley germplasm and accurate positioning of major effective genes, and the phenotype detection is efficient and lossless, the molecular marker selection is accurate, and the present application can also be applied to other cereal crops.

The invention relates to a corn WRKY transcription factor ZmWRKY74 as well as a coding gene and application thereof, and belongs to the technical field of plant gene breeding, the CDS sequence of the coding gene of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.1, the gene negatively regulates the drought tolerance of crop plants, and the amino acid sequence of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.3. Gene positioning analysis is carried out on a drought-resistant mutant of corn, it is found that the ZmWRKY74 gene is an effect gene capable of improving the tolerance of corn to drought stress, and through a ZmWRKY74 gene overexpression experiment, the negative regulation effect of the ZmWRKY74 gene on the drought tolerance of corn plants is verified; important theoretical significance and application value are provided for creation of new corn germplasm and molecular breeding of corn.

The invention belongs to the field of crop heredity, and relates to application of a polyphenol oxidase gene. The invention discloses an InDel marker for rice brown glume traits, a regulatory gene and application of the InDel marker and the regulatory gene. Based on a brown glume mutant material in the background of Hunan early indica 42, genetic analysis shows that the mutation is dominant mutation, single-gene control and gene positioning find that the mutant gene is a polyphenol oxidase encoding gene (MSULocus is LOCOs04g53300), and the exon region of the mutant gene is deleted by 6bp (GCGACT), so that the material shows brown glume; the gene is knocked out from a brown glume mutant, and the glume color becomes normal; the method has the advantages that the GCGACTs are used as the raw materials, the GCGACTs are accurately edited in the wild type materials, the corresponding 6bp deletion (GCGACT) materials are obtained, the glumes of the GCGACTs are brown, and new gene resources and methods are provided for quickly and directionally cultivating novel rice materials and rice materials suitable for mechanized seed production of hybrid rice.

This invention provides a gene prediction and identification method, apparatus, device, and storage medium, belonging to the field of gene annotation and prediction. The method includes: acquiring the gene text to be annotated and a set of promoter-terminator pairs; preprocessing the gene text to generate a raw genome sequence; searching the raw genome sequence for base sequences that fuzzy search matches the set of promoter-terminator pairs to generate a base sequence to be aligned; and comparing the base sequence to be aligned with base sequences in a bioinformatics database based on the BLAST gene alignment method to generate gene prediction and identification results. This invention, by separating base sequences from promoter-terminator pairs and then performing BLAST gene sequence alignment, achieves coarse gene localization followed by BLAST gene sequence alignment, providing a method for predicting new genes, improving alignment efficiency and comprehensiveness, as well as the accuracy and comprehensiveness of gene prediction results.

This invention discloses the cloning and application of the wheat broad-spectrum multi-effect disease resistance gene WAI4. This invention provides a protein, which is as follows: A1) or A2) or A3): A1) a protein comprising the amino acid sequence shown in SEQ ID No. 2; A2) a protein derived from A1) by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in SEQ ID No. 2, and having the same function; A3) a fusion protein obtained by adding a tag protein to the end of the protein shown in A1). This invention provides gene localization, map-based cloning, and biological function identification methods for the wheat stress resistance gene WAI4, demonstrating that the gene WAI4 has resistance to powdery mildew and / or stripe rust, playing an important role in improving and enhancing germplasm resources of crops such as wheat.

This invention application discloses a gene regulating maize kernel development. ZmRH51 This application focuses on the encoded proteins, functional markers, and applications of maize kernel mutants. m223 Using this as the basic material, its heterozygotes were crossed with Zheng 58 (Z58) to construct the F2 segregating population. Phenotypic identification and cytological observation were performed on the progeny segregating population. Controlling populations were obtained through genetic analysis, BSR-seq analysis, and map-based cloning. m223 Genes with mutant phenotypes ZmRH51 This gene is located in the nucleolar region and encodes an RNA helicase belonging to the DEAD-Box gene family. Studies have shown that... ZmRH51 It participates in ribosome assembly and can influence the processing of pre-rRNA in maize kernels; and further mining yields... ZmRH51 The superior allelic variant genotype of this gene in terms of grain thickness can be utilized to improve grain thickness and 100-grain weight, thereby increasing yield. Furthermore, the distribution and utilization potential of different haplotypes in inbred lines were studied, providing technical support and excellent germplasm resources for the utilization of this gene.

The invention discloses a wheat grain weight gene WGY1 as well as an encoded protein and application thereof, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to regulate and control the grain weight of wheat. To this end, the present application provides proteins and related biomaterials that modulate plant yield. The invention also provides application of the protein and related biological materials thereof in regulation and control of plant yield. According to the application, the WGY1 gene is finally obtained through gene localization and map-based cloning. The gene and the protein coded by the gene can be widely applied to the plant fields of wheat genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and play an important role in improving germplasm resources of crops such as wheat and the like.

The invention provides a gene BnaPT13 and application thereof, the nucleotide sequence of the gene BnaPT13 is shown as SEQ ID NO: 1, the gene is positioned on a cell membrane, has a transmembrane phosphorus transport effect, and can improve the utilization of rape phosphorus. The invention provides a new theoretical basis and genetic resources for cultivation of rape varieties.

The invention discloses a rice tillering angle and tillering number control gene LAZY5 and application thereof, and belongs to the technical field of biological breeding. The invention discloses a method for regulating and controlling the tillering angle and / or the tillering number of a plant, and the method comprises the step of regulating and controlling the expression quantity of a coding gene of LAZY5 protein in a receptor plant so as to regulate and control the tillering angle and / or the tillering number of the receptor plant, the LAZY5 protein is a protein of which the amino acid sequence is as shown in SEQ ID No. 2. According to the application, the coding gene of the LAZY5 protein is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle and / or the tillering number. The rice gene LAZY5 can be widely applied to the plant fields of rice genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and plays an important role in improving germplasm resources of crops such as rice and the like.

The invention relates to the technical field of gene chips, in particular to a sorghum whole genome liquid phase chip as well as a construction method and application thereof. A detection target of the sorghum whole genome liquid phase chip disclosed by the invention is composed of 5446 SNP molecular markers and 3 InDel molecular markers; the positions of the 5446 SNP molecular markers and the 3 InDel molecular markers, which are positioned on a sorghum reference genome Sorghumbicolv3.1. 1, are as shown in a table 1; the sorghum whole genome liquid chip can be used for sorghum variety identification, genetic relationship identification, genetic diversity analysis, molecular genetic map construction, gene positioning and cloning, molecular marker-assisted selection and whole genome selective breeding, and provides an important molecular basis for important agronomic trait analysis and biological breeding of sorghum.

The invention relates to a method for rapidly carrying out BSA (Bovine Serum Albumin) gene localization by utilizing a space mutation mutant. The method comprises the following steps: firstly, carrying propagules such as seeds through an aerospace craft, and constructing a broad-spectrum mutant library by utilizing space environment mutagenesis; returning to the ground, culturing and screening M1-generation mutants with target excellent characters, and hybridizing / backcrossing with a wild type to construct a character segregation population; by adopting a group separation analysis (BSA) strategy, selecting phenotype polar differentiation individuals, and respectively mixing the phenotype polar differentiation individuals to form a high-value pool and a low-value pool; and finally, carrying out whole genome high-throughput sequencing on the double pools and the parents, rapidly locking a genome region linked with a target character by combining bioinformatics analysis such as SNP-index and the like, and verifying candidate genes through gene annotation, molecular marking and gene editing. According to the invention, the broad spectrum of space mutagenesis and the high efficiency of the BSA-seq technology are integrated, the period from mutation creation to gene identification is remarkably shortened, and an innovative technical scheme is provided for crop genetic improvement and functional genomics research.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW4; the KASP marker RW4 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 537543. According to the application, the KASP molecular marker RW4 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW4 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a detection marker of a corn plant type regulatory gene and application of the detection marker. Belongs to the technical field of molecular breeding. The corn dwarf mutant d16 is subjected to phenotype identification, genetic analysis and gene positioning, and finally, the ZmD16 gene is found to be a new allele of a known gene Br2. According to the mutant, G > T single base mutation occurs at 3533bp at the downstream of a fifth exon ATG in a coding region of a wild type ZmD16 gene. A KASP molecular marker is designed according to a mutation site. A foundation is laid for deeply analyzing a molecular mechanism of a corn plant type, and a new material is provided for breeding application of density-tolerant varieties.

The invention belongs to the technical field of plant genetic engineering and molecular breeding, and particularly discloses a key negative regulation gene GhHSFC1 for regulating verticillium wilt resistance of cotton and application of the key negative regulation gene GhHSFC1. The gene is positioned on a chromosome A05 of upland cotton of a genetic standard line TM-1, the coding sequence (CDS) of the gene is shown as SEQ ID NO. 1, and the sequence of a protein coded by the gene is shown as SEQ ID NO. 2. Through bioinformatics analysis and in combination with a core virus-induced gene silencing (VIGS) technology, functional verification is carried out, and it is confirmed for the first time that the GhHSFC1 gene is a negative regulatory factor of cotton verticillium wilt resistance. According to the gene resource and the application thereof provided by the invention, a novel target spot subjected to functional verification and a precise and efficient improvement strategy are provided for molecular breeding of cotton verticillium wilt resistance, and the gene resource has important research value and application prospect.

The invention discloses a brassica juncea KASP molecular marker combination and application thereof in germplasm resource identification and breeding, and belongs to the technical field of molecular markers. The mustard KASP molecular marker set is developed from 193 mustard germplasm resources based on whole genome re-sequencing, the mustard KASP molecular marker can quickly, accurately and effectively identify and evaluate germplasm resource materials or bred varieties, and the problems of resource redundancy, inconvenient storage and management, intellectual property disputes and the like caused by accumulation of a large amount of germplasm can be solved. The molecular marker is provided for genetic map construction, gene positioning and molecular assisted breeding.

The application provides cloning of a soybean oil content regulating gene and its encoded protein and application, and relates to the fields of plant molecular genetics and genetic engineering. The soybean oil content regulating gene is qOil10, and the nucleotide sequence is shown in the sequence table SEQ ID NO:1. A major gene controlling soybean oil content is successfully cloned, the function of the gene in positively regulating seed oil accumulation is determined, and the shortage of insufficient soybean oil functional gene resources is filled. The application further provides gene positioning primers, knockout primers, recombination vectors and genetic transformation methods, which can be directly applied to molecular marker assisted selection breeding, significantly improve the breeding efficiency of high-oil soybeans, and shorten the breeding cycle. Compared with conventional breeding, the application is precise in trait improvement and simple in operation, and provides key technical support for cultivating new high-oil soybean varieties and ensuring oil supply safety.

The invention provides a saline-alkaline tolerant rice genome breeding and detection chip and application thereof, the chip comprises 5450 SNP sites distributed in 12 chromosomes, 11080027 initial sites are screened according to the principle that the heterozygosis rate is less than or equal to 15% and the deletion rate is less than or equal to 10%, and the probe design meets the requirements that the length is 110nt and the GC content is 30-70%; the 5K liquid phase chip developed on the basis of a GBTS technology and a GenoBaits method can be used for saline-alkaline tolerant rice germplasm resource screening, genome selective breeding and rice variety identification, molecular marker breeding is assisted through genotype identification and detection, the problems of inaccurate saline-alkaline tolerant gene positioning and low detection efficiency in Heilongjiang traditional breeding are solved, and the application prospect is wide. The combination of traditional and modern technologies is promoted, technical support is provided for molecular breeding and seed quality monitoring of cold region rice, and the method has important significance for improving local rice quality and guaranteeing grain safety.

The invention discloses a gene for controlling existence of wheat tannin and a molecular marker and application thereof. The gene is positioned in a region of 701.8 to 704.3 Mb of a chromosome 3A of a reference genome of wheat in spring in China. According to a generic genome and gene expression result (RNA-seq), a candidate gene is locked and named as TaMYB10-3A, three primer markers are designed on the gene to form a primer combination, and the primer combination can be used for detecting whether wheat contains tannin or not, so that a new means is provided for identifying wheat tannin, and the application prospect is broad. The molecular marker primer combination can be applied to wheat tannin breeding practice in a simple, convenient, rapid and high-throughput mode, the wheat tannin character improvement process is accelerated, and the breeding accuracy and selection efficiency are improved.

The present invention relates to the technical field of molecular detection. Disclosed are a Camellia sinensis liquid-phase chip and the use thereof. Disclosed in the present invention are a Camellia sinensis liquid-phase chip and the use thereof. According to locus screening requirements and probe design principles, the chip comprises 5781 SNP loci, and can achieve the genotyping of Camellia sinensis resources on the basis of a precise positioning sequence-based typing technology via the liquid-phase capture of a genomic sequence at a target interval. The Camellia sinensis liquid-phase chip of the present invention can enable low-cost genotyping, and is mainly specific to Camellia sinensis. The chip can realize cultivar identification and genetic relationship analysis of Camellia sinensis, can provide scientific guidance for Camellia sinensis hybridization and improvement programs, and can facilitate the conservation and exploitation of Camellia sinensis germplasm resources, and thus has a high application value in multiple fields of Camellia sinensis breeding. The Camellia sinensis liquid-phase chip of the present invention can be used for genetic diversity evaluation of Camellia sinensis, germplasm resource and genetic relationship identification, genetic map construction and gene mapping, genome-wide association studies, and molecular marker-assisted breeding of Camellia sinensis.

This invention provides a method for preparing sweet and waxy maize with the same kernel size and its application, relating to the field of bio-breeding technology. This invention identifies and obtains for the first time a new sweet and waxy maize germplasm material, TZNB25341, formed by natural mutation. For the aforementioned new material TZNB25341, this invention conducts in-depth analysis of its key controlling genes for sweet and waxy maize with the same kernel size, and carries out systematic gene mapping and cloning studies on the genetic regulatory mechanism of this trait. It was discovered that in TZNB25341… ZmSH1 A 5979 bp retrotransposon LTR was inserted into the exon 13 region of the (Zm00001eb374090) gene. The insertion of this transposon led to three premature translation termination sequences and a significant decrease in transcription level in the exon, resulting in phenotypic changes in TZNB25341, which ultimately manifested as the sweet and sticky phenotype.

The application discloses a soybean SNP molecular marker combination, a chip and application thereof, relates to the field of soybean molecular genetics and breeding. The SNP molecular marker combination developed by the application is uniformly distributed on 20 chromosomes of the whole genome of soybean, and can improve the accuracy of related research and application of gene positioning. The SNP molecular marker combination provided by the application has rich information, and has important value in variety identification, correlation analysis and molecular marker assisted breeding. Based on this, a SNP typing chip, a kit and other detection products with high capture rate, high density, high polymorphism and uniform distribution in the genome can be developed.

The invention discloses a rice gene OsLTD1 and application thereof. The CDS sequence of the rice gene OsLTD1 is as shown in SEQ ID NO.1, and the amino acid sequence of encoding protein of the rice gene OsLTD1 is as shown in SEQ ID NO.3. The invention further discloses a preparation method of the rice gene OsLTD1. Through gene localization and function complementation verification technologies, it is identified that function deletion of the OsLTD1 gene can significantly enhance the resistance of rice to rice blast. The function deletion mutant osltd1 of the gene shows leaf yellowing and lesion-like phenotypes from a seedling stage to a tillering stage, and shows broad-spectrum resistance to a plurality of pyricularia grisea physiological races. According to the invention, the broad-spectrum disease-resistant character and the excellent agronomic character are efficiently combined through a visual leaf color marker associated with gene function deletion, a new high-yield and disease-resistant rice variety is finally cultivated, and a new technical scheme and a new gene resource are provided for solving the problem of rice blast prevention and treatment.

The invention belongs to the technical field of biological genetic engineering, and particularly relates to a corn kernel development regulation gene ZmPPR299, an encoding protein thereof, an SNP (Single Nucleotide Polymorphism) site, a molecular marker and application of the molecular marker, the gene ZmPPR299 is located in a corn chromosome 5, and functional verification shows that the gene encodes a PPR protein located in chloroplast, and the molecular marker can be used for regulating the development of corn kernels. The chloroplast gene is an essential gene for regulating corn kernel development and chloroplast functions; the mutation of the gene can cause the lagging of embryo and endosperm development, the reduction of basal transfer layer cell proliferation, and the insufficient filling of starch grains and protein bodies, and finally, the results show that the grains shrink, the hundred-grain weight is obviously reduced, and the leaves of seedlings lose green. The invention also develops a specific CAPS molecular marker and a detection primer thereof based on a single base mutation site of the gene, and the genotype of the mutant can be rapidly and accurately identified. The gene resource and the molecular marker provided by the invention have important application values in analysis of a corn kernel development regulation network, development of molecular assisted breeding and creation of high-yield and high-quality new corn germplasm.

The invention discloses a KASP molecular marker linked with a loose cauliflower globule gene and application, and belongs to the technical field of molecular breeding. Specifically, a high-generation inbred line material and a globule mutant obtained through EMS mutagenesis of the high-generation inbred line material are utilized to construct a segregation population for gene localization, a chromosome region closely linked with globule characters is obtained, and a corresponding molecular marker is developed in a candidate region. A KASP molecular marker is further designed and verified for a key single base mutation site of a candidate gene. Through verification, in genotype identification of 550 single plants, the coincidence rate of the marker with large and small ball phenotypes reaches 100%. The molecular marker can be effectively applied to molecular marker-assisted selective breeding of loose broccoli with big and small ball characters, and the selection accuracy and the breeding efficiency are remarkably improved; meanwhile, an important tool and a genetic basis are provided for cloning and functional analysis of related genes of the globules.

The application discloses a SSR core primer group based on whole genome sequencing and core germplasm resequencing of Glycine max and application thereof. Specifically disclosed is the SSR core primer group, which comprises at least 5 pairs of primers (SEQ ID No. 1-460) in 230 pairs of primers. The application establishes a method for developing the whole genome SSR marker of Glycine max, which is comprehensive, accurate and reliable, and solves the problems of the current Glycine max SSR marker, such as small quantity, low polymorphism and low genome coverage. The SSR core primer group can maximize the reflection of the genetic diversity of Glycine max germplasm resources, has the characteristics of high polymorphism, uniform distribution, stable amplification, good reproducibility, clear site, clear and easy-to-distinguish statistical electrophoresis bands and the like, can accurately perform kinship analysis and diversity analysis, and is helpful to the researches such as genetic map construction, gene positioning, variety identification and variety DNA fingerprint construction, and has a wide application prospect.

This invention discloses the cloning and application of the wheat broad-spectrum powdery mildew resistance gene Pm26. The invention provides the Pm26 protein, a protein composed of the amino acid sequence shown in SEQ ID No. 3; it also provides the gene Pm26, a DNA molecule with the coding region shown in positions 3001-9489 of SEQ ID No. 1, or a DNA molecule with the coding region shown in SEQ ID No. 2; this invention provides gene localization, map-based cloning, and methods for identifying the biological function of the wheat broad-spectrum powdery mildew resistance gene Pm26. The wheat broad-spectrum powdery mildew resistance gene Pm26 can be widely used in plant fields such as wheat disease resistance genetic breeding, germplasm resource improvement, transgenic and genome editing breeding, and plays an important role in improving and enhancing the germplasm resources of crops such as wheat.

The application discloses application of a KASP molecular marker in identification of a melon fruit peel color character and belongs to the technical field of molecular markers. The application aims to use molecular marker assisted selection breeding in the melon fruit peel color character so as to promote gene positioning and the molecular assisted breeding process. The KASP molecular marker is a KASP marker RW4, and the KASP marker RW4 is designed according to a SNP site at Chr04:537543. The application uses the KASP molecular marker RW4 to genotype red fruit peel melons and white fruit peel melons, and only needs to amplify the target plant DNA through one-time PCR, and the whole detection process is simple, and complex steps such as enzyme cutting, electrophoresis and sequencing are not needed. Through a high-throughput genotyping system, a genotyping map and a typing value can be rapidly obtained, so that the rapid identification of the genotype of the target plant can be realized. The KASP molecular marker RW4 in the application can be more efficiently applied to the breeding of melon fruit peel color germplasm.

The application discloses cloning and application of a wheat broad-spectrum polygene WAI-A1 and belongs to the technical field of genetic engineering breeding. The technical problem to be solved by the application is how to improve the stress resistance of plants, for example, how to improve the disease resistance of wheat. In order to solve the above technical problem, in the first aspect, the application provides application of a protein or a substance for regulating gene expression or a substance for regulating the activity or content of the protein in the regulation of the stress resistance of plants, wherein the gene codes the protein, and the protein is WAI-A1 protein. The application provides a gene positioning, map-based cloning and biological function identification method of the wheat stress resistance gene WAI-A1. The wheat stress resistance gene WAI-A1 can be widely applied to the fields of wheat disease resistance genetic breeding, germplasm resource improvement, transgenic and genome editing breeding and the like, and has an important role in improving and improving the germplasm resources of crops such as wheat.

The application discloses cloning of a wheat hybridization necrosis gene Ne1 and development of a functional marker. The application provides a protein consisting of an amino acid sequence shown in SEQ ID No. 3. A nucleic acid molecule encoding the protein is also provided, and is named as a Ne1 gene, which is a DNA molecule shown in SEQ ID No. 2, or a DNA molecule shown in SEQ ID No. 1 or 2407-10,563 of SEQ ID No. 1. The application provides gene location, map-based cloning, mutant and transgenic function verification and development of a functional marker of the wheat hybridization necrosis gene Ne1. Cloning of the wheat hybridization necrosis gene Ne1 can completely reveal the mystery of wheat hybrid necrosis formation, can provide theoretical guidance for overcoming genetic obstacles of hybridization necrosis in the process of wheat hybridization breeding, can promote cultivation of new wheat varieties with high efficiency and excellent traits, and has important significance.