72 results about "Gene Localization" patented technology

The embodiment of the invention discloses a KASP molecular marker, a primer pair and a kit for identifying the dwarf character of muskmelon and application of the KASP molecular marker, relates to the technical field of molecular markers, and aims to promote gene positioning and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on the dwarf character of muskmelon. The method for identifying the dwarf character of the muskmelon comprises the following steps: extracting genome DNA of a target muskmelon, taking the genome DNA of the target muskmelon as a template for PCR amplification, and performing fluorescence detection based on a primer of a KASP marker K1069930 to obtain a gene type of the target muskmelon, on the basis of the detected gene type, the character of the target muskmelon is judged; the KASP marker K1069930 is designed according to an SNP (Single Nucleotide Polymorphism) site at Chr07: 1069930, and the nucleotide sequence of the SNP site is shown as SEQ ID NO. 1. The invention also discloses a kit for detecting the KASP marker K1069930 and a kit for detecting the KASP marker K1069930. The identification method provided by the invention can be more efficiently applied to breeding work of dwarf muskmelon germplasm.

The invention relates to the technical field of crop genetic breeding and plant molecular design breeding, in particular to a pakchoi whole genome liquid phase chip and application. The pakchoi breeding gene chip disclosed by the invention comprises a target capture probe which is designed by taking 1000 core SNP (Single Nucleotide Polymorphism) molecular markers positioned on 40KSNP sites on a pakchoi reference genome version V4.0 as templates. The pakchoi whole genome liquid phase chip provided by the invention comprises a 40K site targeted capture probe mixed solution and a hybridization capture reagent. The pakchoi 40KSNP targeted capture probe can rapidly and effectively track genetic materials of pakchoi, and is suitable for different application scenes such as accurate identification of pakchoi germplasm resources, genetic relationship analysis between materials, variety authenticity identification, QTL gene positioning and mining, molecular marker-assisted breeding, variety improvement and whole genome selective breeding application. Therefore, the method has good application prospect and important social value.

The invention discloses a linked molecular marker of a major QTL (quantitative trait loci) uq2 of a peanut legume singulation character as well as an identification method and application of the linked molecular marker. The molecular marker and a major QTL site uq2 of the peanut legume singulation character are co-localized on a second chromosome of a peanut genome, and the molecular marker comprises molecular markers SNP4 and SNP57. The physical position of the molecular marker SNP4 is the 11591222th base position of the second chromosome of a cultivated peanut genome, and the polymorphism is C / T; the physical position of the molecular marker SNP57 is the 11894442 base position of the second chromosome of the cultivated peanut genome, and the polymorphism is A / T. The linked molecular marker is obtained by using a primer group. The nucleotide sequences of the primer group are respectively as shown in SEQ ID No. 1 to SEQ ID No. 6. The major QTL locus uq2 of the peanut legume constriction character as well as the linked molecular marker and the identification method of the major QTL locus uq2 are suitable for gene localization of the peanut legume constriction character and molecular marker-assisted selective breeding.

The invention discloses application of wheat Rht1-D1b protein in regulation and control of a wheat tillering angle, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to increase the tillering angle of plants. Therefore, the invention provides the application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the tillering angle of the plant, wherein the protein can be Rht1-D1b protein; the Rht1-D1b protein can be a protein of which the amino acid sequence is as shown in SEQ ID No. 3. According to the application, the Rht1-D1b gene is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle of wheat. The wheat gene Rht1-D1b can be widely applied to the plant fields of wheat germplasm resource improvement, wheat plant type genetic breeding and the like, and has an important application value for improving the wheat yield.

The invention discloses an SSR (simple sequence repeat) molecular marker associated with fresh weight character of corydalis tuber bulbules as well as primers and application of the SSR molecular marker, and belongs to the technical field of molecular genetics. According to the SSR molecular marker associated with the fresh weight character of the corydalis tuber bulbous disclosed by the invention, the amplification primer sequences of the SSR molecular marker are shown as SEQ ID NO.1 and SEQ ID NO.2. The SSR molecular marker associated with the fresh weight character of the rhizoma corydalis coleobulb and the amplification primer thereof can be applied to rhizoma corydalis population structure analysis, rhizoma corydalis coleobulb fresh weight character associated marker analysis, rhizoma corydalis excellent germplasm breeding and preparation of a kit for screening rhizoma corydalis excellent germplasm with high coleobulb weight. The method can be directly used for excellent germplasm screening, gene localization and cloning and molecular marker-assisted breeding, has chromosome localization information, is beneficial to further fine localization and cloning of genes for controlling fresh weight characters of corpus balls, and has important significance in breeding of high-yield rhizoma corydalis.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

The invention discloses a corn kernel development regulation gene ZmRH51 as well as an encoding protein, a functional marker and an application of the corn kernel development regulation gene ZmRH51. According to the application, a corn kernel mutant m223 is taken as a basic material, heterozygote of the corn kernel mutant m223 is hybridized with Zheng 58 (Z58) to construct an F2 segregation population, then phenotype identification and cytological observation are performed on the segregation population of the offspring, the gene ZmRH51 for controlling the mutation phenotype of the m223 is obtained through genetic analysis, BSR-seq analysis and map-based cloning, the gene is positioned in a nucleolus region, and the mutation phenotype of the m223 is controlled. The ZmRH51 is used for coding RNA helicase of a DEAD-Box gene family, and research shows that ZmRH51 participates in ribosome assembly and can influence processing of pre-rRNA in corn kernels; the excellent allelic variation genotype of the excavated ZmRH51 gene in the aspect of grain thickness is obtained through further excavation, the grain thickness and hundred-grain weight of the ZmRH51 gene can be increased through the utilization of the gene, and then the yield is increased. In addition, distribution and utilization potentials of different haplotypes in a selfing line are researched, and technical support and excellent germplasm resources are provided for utilization of the gene.

Cloning of a wheat broad-spectrum powdery mildew-resistant gene Pm26 and a use thereof. A Pm26 protein is a protein composed of the amino acid sequence represented by SEQ ID No. 3. Also provided is a gene Pm26, wherein a coding region is DNA molecules represented by positions 3001-9489 in SEQ ID No. 1, or the coding region is DNA molecules represented by SEQ ID No. 2. Further provided are gene mapping, map-based cloning and powdery mildew-resistant biological function identification methods for the wheat broad-spectrum powdery mildew-resistant gene PM26. The wheat broad-spectrum powdery mildew-resistant gene Pm26 can be widely used in plant fields of wheat disease-resistant genetic breeding, germplasm resource improvement, transgenic and genome editing breeding and the like, and plays an important role in improving and enhancing the germplasm resources of crops such as wheat.

The invention relates to the technical field of gene chips, in particular to a masson pine SNP molecular marker combination and application thereof. The SNP molecular marker combination is composed of 113 SNP molecular markers and 709 SNP molecular markers, wherein the physical positions of the SNP molecular markers are determined by carrying out sequence alignment on the basis of a reference genome Pinus.tabuliformes V1.0 of the Chinese pines. According to the embodiment of the invention, the masson pine whole genome chip containing 113 and 709 SNP sites is provided for the first time, and has the advantages of high efficiency, low cost, good hereditary stability and the like; the method can be widely applied to different application scenes such as pinus massoniana germplasm resource identification, breeding material genetic background analysis, gene localization, whole-genome association analysis, whole-genome selective breeding and intelligent design breeding.

The invention discloses a biological material for regulating and controlling effective tillering related protein ETN1 of wheat and application of the biological material, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to improve the effective tiller number of plants and / or reduce the plant height. Therefore, the invention provides the application of the ETN1 protein or a substance for regulating the expression of an encoding gene of the ETN1 protein or a substance for regulating the activity or content of the ETN1 protein in regulating the tiller number or the plant height of the plant, and the ETN1 protein can be a protein with an amino acid sequence as shown in a sequence 2 in a sequence table. The gene of the ETN1 protein is finally localized through gene localization and map-based cloning. The gene can be widely applied to the plant fields of wheat genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and plays an important role in improving germplasm resources of crops such as wheat and the like.

The present application relates to the field of crop genetic breeding and molecular biology, and particularly relates to a highland barley beta-glucan screening index system and a major gene positioning method, which comprises the following steps: firstly, a three-dimensional coupling screening system of genetic stability, functional activity and agronomic adaptability is constructed, the index combination weight is determined by using an entropy weight method and an analytic hierarchy process, and an AMMI model is combined to correct the environmental effect and calculate the comprehensive score of the germplasm; secondly, a recombinant inbred line and a natural germplasm double positioning population is constructed, and near-infrared spectroscopy is combined with a Transformer model to quickly obtain phenotype data; thirdly, a candidate major effective segment is obtained through double population joint positioning, a major effective gene is screened out by combining a transcriptome and a metabolome co-expression network, and a functional molecular marker is developed. The present application realizes accurate evaluation of highland barley germplasm and accurate positioning of major effective genes, and the phenotype detection is efficient and lossless, the molecular marker selection is accurate, and the present application can also be applied to other cereal crops.

The present invention discloses the use of the MYC2 gene of Pansy as a positive regulatory factor in promoting the release of plant ecodormancy, relating to the field of biotechnology. The present invention, for the first time, demonstrates the important role of the MYC2 gene in releasing ecodormancy in Pansy by transiently overexpressing a homologous gene. The invention also confirms that the gene is localized in the cell nucleus and that its expression increases with the release of ecodormancy. Overexpression of the MYC2 gene in Pansy promotes the release of plant ecodormancy and germination.

The present invention relates to the gene cloning and functional application of the salt-tolerant gene Hg52609 in the roots of Halogeton glomeratus, belonging to the field of plant bioengineering technology. The salt-tolerant gene Hg52609 was cloned from the roots of Halogeton glomeratus for the first time. By cloning the salt-tolerant related gene Hg52609 in the roots of Halogeton glomeratus, a subcellular localization expression vector was constructed, and gene localization was carried out through transient expression in tobacco; an overexpression vector was constructed, and Arabidopsis thaliana was stably genetically transformed for salt tolerance identification; a yeast heterologous expression vector was constructed to further verify the function of the Hg52609 gene. It was found that the Hg52609 gene is involved in the efflux of Na+ but does not mediate the absorption of K+ under high concentrations of Na+. The present invention provides the cloning and functional application of the salt-tolerant gene Hg52609 in the roots of Halogeton glomeratus, which will be a good material for exploring plant salt-tolerant genes and studying the salt-tolerant regulation mechanism, and has important application value for improving the stress resistance of crops and cultivating salt-tolerant and alkali-tolerant crops.

The invention relates to the technical field of molecular genetic breeding, and discloses a soybean plant type gene cloning and multiple PCR (Polymerase Chain Reaction) rapid identification method and application, comprising a PCR detection method of a stem apical clustered pod material and a stem apical normal pod material, molecular markers for gene positioning, cloning and plant type identification, and a PCR specific reaction system and conditions. The detection method can be applied to the fields of variety resource identification, transgenic breeding, gene editing breeding, molecular marker selective breeding, gene function research and the like. The method has the advantages of economy, rapidness, simplicity in operation and the like.

The invention relates to a corn WRKY transcription factor ZmWRKY74 as well as a coding gene and application thereof, and belongs to the technical field of plant gene breeding, the CDS sequence of the coding gene of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.1, the gene negatively regulates the drought tolerance of crop plants, and the amino acid sequence of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.3. Gene positioning analysis is carried out on a drought-resistant mutant of corn, it is found that the ZmWRKY74 gene is an effect gene capable of improving the tolerance of corn to drought stress, and through a ZmWRKY74 gene overexpression experiment, the negative regulation effect of the ZmWRKY74 gene on the drought tolerance of corn plants is verified; important theoretical significance and application value are provided for creation of new corn germplasm and molecular breeding of corn.

The invention belongs to the field of crop heredity, and relates to application of a polyphenol oxidase gene. The invention discloses an InDel marker for rice brown glume traits, a regulatory gene and application of the InDel marker and the regulatory gene. Based on a brown glume mutant material in the background of Hunan early indica 42, genetic analysis shows that the mutation is dominant mutation, single-gene control and gene positioning find that the mutant gene is a polyphenol oxidase encoding gene (MSULocus is LOCOs04g53300), and the exon region of the mutant gene is deleted by 6bp (GCGACT), so that the material shows brown glume; the gene is knocked out from a brown glume mutant, and the glume color becomes normal; the method has the advantages that the GCGACTs are used as the raw materials, the GCGACTs are accurately edited in the wild type materials, the corresponding 6bp deletion (GCGACT) materials are obtained, the glumes of the GCGACTs are brown, and new gene resources and methods are provided for quickly and directionally cultivating novel rice materials and rice materials suitable for mechanized seed production of hybrid rice.

The present invention discloses a molecular marker for rapeseed black rot, a method for obtaining the marker and an application thereof. Rapeseed black rot resistant material is identified by inoculation through leaf clipping method, an F2 population of the material and susceptible source material is constructed, and the disease resistance gene is initially located by BSA-seq technology, and SNP and InDel molecular markers that can be used for black rot resistance screening and gene localization are screened within this interval. The molecular markers include SNP molecular markers 1 to 9 and InDel molecular markers 1 to 4. The present invention provides new molecular markers for the excavation of rapeseed black rot functional genes and molecular breeding, and has important application prospects for enhancing rapeseed resistance to black rot.

This invention provides a gene prediction and identification method, apparatus, device, and storage medium, belonging to the field of gene annotation and prediction. The method includes: acquiring the gene text to be annotated and a set of promoter-terminator pairs; preprocessing the gene text to generate a raw genome sequence; searching the raw genome sequence for base sequences that fuzzy search matches the set of promoter-terminator pairs to generate a base sequence to be aligned; and comparing the base sequence to be aligned with base sequences in a bioinformatics database based on the BLAST gene alignment method to generate gene prediction and identification results. This invention, by separating base sequences from promoter-terminator pairs and then performing BLAST gene sequence alignment, achieves coarse gene localization followed by BLAST gene sequence alignment, providing a method for predicting new genes, improving alignment efficiency and comprehensiveness, as well as the accuracy and comprehensiveness of gene prediction results.

This invention discloses the cloning and application of the wheat broad-spectrum multi-effect disease resistance gene WAI4. This invention provides a protein, which is as follows: A1) or A2) or A3): A1) a protein comprising the amino acid sequence shown in SEQ ID No. 2; A2) a protein derived from A1) by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in SEQ ID No. 2, and having the same function; A3) a fusion protein obtained by adding a tag protein to the end of the protein shown in A1). This invention provides gene localization, map-based cloning, and biological function identification methods for the wheat stress resistance gene WAI4, demonstrating that the gene WAI4 has resistance to powdery mildew and / or stripe rust, playing an important role in improving and enhancing germplasm resources of crops such as wheat.

This invention application discloses a gene regulating maize kernel development. ZmRH51 This application focuses on the encoded proteins, functional markers, and applications of maize kernel mutants. m223 Using this as the basic material, its heterozygotes were crossed with Zheng 58 (Z58) to construct the F2 segregating population. Phenotypic identification and cytological observation were performed on the progeny segregating population. Controlling populations were obtained through genetic analysis, BSR-seq analysis, and map-based cloning. m223 Genes with mutant phenotypes ZmRH51 This gene is located in the nucleolar region and encodes an RNA helicase belonging to the DEAD-Box gene family. Studies have shown that... ZmRH51 It participates in ribosome assembly and can influence the processing of pre-rRNA in maize kernels; and further mining yields... ZmRH51 The superior allelic variant genotype of this gene in terms of grain thickness can be utilized to improve grain thickness and 100-grain weight, thereby increasing yield. Furthermore, the distribution and utilization potential of different haplotypes in inbred lines were studied, providing technical support and excellent germplasm resources for the utilization of this gene.

The present invention discloses a 60K SNP liquid breeding chip for broiler chickens, which relates to the field of biological detection technology. The 60K SNP liquid breeding chip for broiler chickens includes a probe combination, which is used to identify the genotypes of 61,219 SNP loci. The functional loci and gene region loci of the 60K SNP liquid breeding chip for broiler chickens provided by the present invention are rich, the chip loci have high polymorphism and are evenly distributed on chromosomes, and it can be widely used in different application scenarios such as the construction of genetic maps of broiler chickens, gene mapping, genome-wide association analysis, molecular marker-assisted selection, directional improvement, mining and identification of genes for important traits and functional analysis, and genome-wide selection breeding for different broiler chicken samples, which is of great significance for improving the breeding efficiency of broiler chickens and promoting the high-quality development of the broiler chicken industry in China.

The invention discloses a wheat grain weight gene WGY1 as well as an encoded protein and application thereof, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to regulate and control the grain weight of wheat. To this end, the present application provides proteins and related biomaterials that modulate plant yield. The invention also provides application of the protein and related biological materials thereof in regulation and control of plant yield. According to the application, the WGY1 gene is finally obtained through gene localization and map-based cloning. The gene and the protein coded by the gene can be widely applied to the plant fields of wheat genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and play an important role in improving germplasm resources of crops such as wheat and the like.

The invention provides a gene BnaPT13 and application thereof, the nucleotide sequence of the gene BnaPT13 is shown as SEQ ID NO: 1, the gene is positioned on a cell membrane, has a transmembrane phosphorus transport effect, and can improve the utilization of rape phosphorus. The invention provides a new theoretical basis and genetic resources for cultivation of rape varieties.

The invention discloses a rice tillering angle and tillering number control gene LAZY5 and application thereof, and belongs to the technical field of biological breeding. The invention discloses a method for regulating and controlling the tillering angle and / or the tillering number of a plant, and the method comprises the step of regulating and controlling the expression quantity of a coding gene of LAZY5 protein in a receptor plant so as to regulate and control the tillering angle and / or the tillering number of the receptor plant, the LAZY5 protein is a protein of which the amino acid sequence is as shown in SEQ ID No. 2. According to the application, the coding gene of the LAZY5 protein is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle and / or the tillering number. The rice gene LAZY5 can be widely applied to the plant fields of rice genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and plays an important role in improving germplasm resources of crops such as rice and the like.

The invention relates to the technical field of gene chips, in particular to a sorghum whole genome liquid phase chip as well as a construction method and application thereof. A detection target of the sorghum whole genome liquid phase chip disclosed by the invention is composed of 5446 SNP molecular markers and 3 InDel molecular markers; the positions of the 5446 SNP molecular markers and the 3 InDel molecular markers, which are positioned on a sorghum reference genome Sorghumbicolv3.1. 1, are as shown in a table 1; the sorghum whole genome liquid chip can be used for sorghum variety identification, genetic relationship identification, genetic diversity analysis, molecular genetic map construction, gene positioning and cloning, molecular marker-assisted selection and whole genome selective breeding, and provides an important molecular basis for important agronomic trait analysis and biological breeding of sorghum.

The invention relates to a method for rapidly carrying out BSA (Bovine Serum Albumin) gene localization by utilizing a space mutation mutant. The method comprises the following steps: firstly, carrying propagules such as seeds through an aerospace craft, and constructing a broad-spectrum mutant library by utilizing space environment mutagenesis; returning to the ground, culturing and screening M1-generation mutants with target excellent characters, and hybridizing / backcrossing with a wild type to construct a character segregation population; by adopting a group separation analysis (BSA) strategy, selecting phenotype polar differentiation individuals, and respectively mixing the phenotype polar differentiation individuals to form a high-value pool and a low-value pool; and finally, carrying out whole genome high-throughput sequencing on the double pools and the parents, rapidly locking a genome region linked with a target character by combining bioinformatics analysis such as SNP-index and the like, and verifying candidate genes through gene annotation, molecular marking and gene editing. According to the invention, the broad spectrum of space mutagenesis and the high efficiency of the BSA-seq technology are integrated, the period from mutation creation to gene identification is remarkably shortened, and an innovative technical scheme is provided for crop genetic improvement and functional genomics research.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW4; the KASP marker RW4 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 537543. According to the application, the KASP molecular marker RW4 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW4 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a detection marker of a corn plant type regulatory gene and application of the detection marker. Belongs to the technical field of molecular breeding. The corn dwarf mutant d16 is subjected to phenotype identification, genetic analysis and gene positioning, and finally, the ZmD16 gene is found to be a new allele of a known gene Br2. According to the mutant, G > T single base mutation occurs at 3533bp at the downstream of a fifth exon ATG in a coding region of a wild type ZmD16 gene. A KASP molecular marker is designed according to a mutation site. A foundation is laid for deeply analyzing a molecular mechanism of a corn plant type, and a new material is provided for breeding application of density-tolerant varieties.

The invention discloses an SNP (Single Nucleotide Polymorphism) site combination for grassiness genotyping, a whole genome liquid phase chip and application of the whole genome liquid phase chip, and belongs to the technical field of gene chips. The SNP locus combination is 11319 SNP loci which are positioned on a grassiness reference genome version GCA028749085.1, and the liquid phase chip genetic typing loci comprise the SNP locus combination. The invention further discloses a detection method of the liquid phase chip genetic typing loci, and the detection method of the liquid phase chip genetic typing loci comprises the SNP locus combination and the detection method of the liquid phase chip genetic typing loci. The pennisetum purpureum whole genome liquid chip provided by the invention provides an efficient genome typing tool for genetic diversity analysis, variety identification, gene localization, background screening and molecular marker-assisted breeding of pennisetum purpureum; meanwhile, a new probe can be designed at any time according to a new site, the requirement for the sample amount is not high, different use scenes and use requirements of users can be met, and high application value is achieved in pennisetum purpureum molecular breeding.