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Method for positioning quantitative trait locus (QTL)

A technology of positioning method and analysis method, which is applied in the field of molecular biology and can solve problems such as inability to clearly group

Inactive Publication Date: 2010-06-30
李祥
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Problems solved by technology

[0002] Since quantitative traits present continuous variation and cannot be grouped clearly, QTL mapping cannot be fully applied to the analysis methods of qualitative trait gene mapping, and special analysis methods must be developed

Method used

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Embodiment Construction

[0010] 1. Marker-based analysis

[0011] If a certain marker is linked to a certain QTL, a certain degree of co-segregation will occur between the marker and the QTL in the hybrid offspring, so in the different genotypes of the marker, the genotype frequency distribution of the QTL (segregation ratio ) will be different, and thus there will be differences in the distribution, mean and variance of the quantitative trait between different genotypes of that marker. It is by examining these differences between different genotypes of a marker that marker-based assays infer whether a marker is linked to a QTL. The current QTL mapping mainly adopts this kind of analysis method. According to the statistical model adopted, the existing marker-based analysis methods can be roughly divided into four types:

[0012] (1) Analysis of variance (ANOVA), also known as single-marker analysis. It was proposed by Thoday in 1961. It compares the differences in the mean values ​​of quantitative...

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Abstract

Because quantitative traits present continuous variation and can not be clearly grouped, a quantitative trait locus (QTL) can not positioned by completely utilizing an analyzing method of qualitative trait gene positioning, and a special analyzing method is necessarily developed. A method for positioning the QTL is researched mainly by an analyzing method based on a mark, an analyzing method based on the traits and a comparative genomic analyzing method.

Description

technical field [0001] The QTL mapping method belongs to the field of molecular biology. Background technique [0002] Since quantitative traits present continuous variation and cannot be clearly grouped, QTL mapping cannot be fully applied to the analysis methods of qualitative trait gene mapping, and special analysis methods must be developed. Contents of the invention [0003] 1. Marker-based analysis [0004] If a certain marker is linked to a certain QTL, a certain degree of co-segregation will occur between the marker and the QTL in the hybrid offspring, so in the different genotypes of the marker, the genotype frequency distribution of the QTL (segregation ratio ) will be different, and thus there will be differences in the distribution, mean and variance of the quantitative trait between different genotypes of that marker. It is by examining these differences between different genotypes of a marker that marker-based assays infer whether a marker is linked to a QT...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李祥
Owner 李祥
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