Compound yolk antibody capable of resisting fowl bacterial blight and its preparation and use

A yolk antibody and bacterial technology, which is applied in the field of yolk antibody substances and compound yolk antibody products, can solve the problems of inability to industrialize production, many restricted conditions, and high impurity content, and achieve the effects of improving quality, improving purity, and being easy to industrialize

Inactive Publication Date: 2002-12-18
重庆和润实业(集团)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problems of high impurity content of biological preparations, poultry and veterinary drugs, ineffective immunoglobulin, many restricted conditions, and impossibility of real industrial production. It effectively improves the purity of immunoglobulin so that it can directly act on the chicken body, Its homology and effectiveness are higher than other biological agents; at the same time, it solves the problem of antibiotic drugs on the residues of meat, poultry and egg products, and provides a favorable guarantee for effectively improving the quality of such products

Method used

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  • Compound yolk antibody capable of resisting fowl bacterial blight and its preparation and use
  • Compound yolk antibody capable of resisting fowl bacterial blight and its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the product of Salmonella chicken, Pasteurella multocida and Escherichia coli compound egg yolk antibody with antibody activity can be obtained by the following steps: preparing the composite antigen of Salmonella chicken, Pasteurella multocida and Escherichia coli, combining the antigen The healthy hens were immunized, and then their eggs were collected, and anti-BAIgY was extracted from the eggs.

Embodiment 2

[0030] Example 2: Preparation of bacterial antigens, pathogenic bacteria strains were expanded and cultivated in nutrient broth medium for 24 hours. Bacterial cells were collected, ultrasonically crushed, and complete Freund's adjuvant was added in a weight ratio of 1:1:1 to emulsify to obtain bacterial cell antigens.

Embodiment 3

[0031] Example 3: Anti-BAIgY was isolated and extracted in the following manner: Egg yolk was isolated from immunized chicken eggs, diluted with distilled water, adjusted to an appropriate pH value and left standing at 4°C for 8 hours, centrifuged to collect the supernatant, concentrated, and freeze-dried to obtain anti-BAIgY.

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Abstract

The present invention relates to a compound vitelline antibody (anti-BAIgY) with antibody activity for resisting salmonella gallinarum, bacterium multocidum and chicken colibacillus, method for preparing said antibody and application of said antibody in preparation of medicine or feed additive for curing relatied diseases which can be infected by above-mentioned bacteria. The preparation method of said compound vitelline antibody includes the following steps: preparing compound antigen of the above-mentioned bacteria, immunizing health hen with said antigen, collecting egg and extracting anti-BA IgY from egg.

Description

technical field [0001] The invention relates to an egg yolk antibody material with antibody activity-a compound egg yolk antibody (anti-BA IgY) product against poultry bacterial disease, and relates to its preparation process and application in poultry pharmacy. technical background [0002] Pullorum is a severe infectious disease caused by Salmonella in poultry of all ages represented by chickens, mainly through the digestive tract infection. Sick chickens or chickens with bacteria in breeding chickens are the main source of infection of the disease, and at the same time they are infected and spread among chickens in the same flock; fowl cholera caused by Pasteurella multocida is a worldwide disease. Escherichia coli is one of the most common pathogenic bacteria in poultry, which can cause a series of manifestations such as poultry embryo death, omphalitis, sepsis, granuloma, yolk peritonitis and panophthalmitis. These diseases can occur throughout the year, and the damage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/02
Inventor 陈君雁杰
Owner 重庆和润实业(集团)有限公司
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