Chemically synthesized HSV1 virus gB glucoprotein extracellular region gene fragment, representation and application of the same

A gene fragment, chemical synthesis technology, applied in the fields of genetic engineering technology, vaccines and diagnostic reagents, can solve the problems of high risk and high production cost
CN101173290AInactive Publication Date: 2008-05-07李袊希
0 Cites 7 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
李袊希
Publication Date
2008-05-07
Estimated Expiration
Not applicable Β· inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The chemically synthesized HSV1 viral gB glycoprotein extracellular region gene fragment and its expression and application relate to the fields of genetic engineering technology, vaccines and diagnostic reagents. The present invention screens out the strong epitope in the gB glycoprotein of HSV1 virus through computer analysis, from the first amino acid to the 696th amino acid, a total of 696 amino acids, selects codons favored by both eukaryotic and prokaryotic organisms, and chemically synthesizes The brand-new gene sequence of the antigenic epitope uses genetic engineering technology to express the gene fragment and prepare a strong antigenic epitope fragment of the gB glycoprotein of the HSV1 virus. The expressed strong antigenic epitope fragment of gB glycoprotein of HSV1 virus can be used for the detection of vaccine, HSV1 virus antibody or antigen, and for immunization preparation of anti-HSV1 virus monoclonal antibody and polyclonal antibody and the like.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to a chemically synthesized brand-new gene fragment of the extracellular region of herpes simplex virus 1 (Herpes simplex virus 1, HSV1) glycoprotein B (glycoprotein B, gB glycoprotein), and uses genetic engineering technology to prepare recombinant HSV1 virus gB glycoprotein. Through computer analysis, the gB glycoprotein extracellular region fragment of HSV1 virus containing strong antigenic epitopes is screened out, the codons favored by both eukaryotic and prokaryotic organisms are selected, and a new gene sequence is chemically synthesized, and expressed by genetic engineering technology. The protein can be used for the detection of vaccines and HSV1 virus antibodies or antigens, etc. The invention relates to the fields of genetic engineering technology, vaccines and diagnostic reagents. Background technique

[0002] Herpes simplex virus (HSV) is a common pathogen that endangers human health. It is easy to cause recu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More