Chemically synthesized HSV1 virus gB glucoprotein extracellular region gene fragment, representation and application of the same

A gene fragment, chemical synthesis technology, applied in the fields of genetic engineering technology, vaccines and diagnostic reagents, can solve the problems of high risk and high production cost

A gene fragment, chemical synthesis technology, applied in the fields of genetic engineering technology, vaccines and diagnostic reagents, can solve the problems of high risk and high production cost

CN101173290AInactive Publication Date: 2008-05-07李袊希
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  • Chemically synthesized HSV1 virus gB glucoprotein extracellular region gene fragment, representation and application of the same
  • Chemically synthesized HSV1 virus gB glucoprotein extracellular region gene fragment, representation and application of the same
  • Chemically synthesized HSV1 virus gB glucoprotein extracellular region gene fragment, representation and application of the same

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0216] 1. Screening of gB glycoprotein epitopes of HSV1 virus and synthesis of gene fragments:

[0217] Utilize software such as ANTHEWIN, analyze the whole amino acid sequence (GeneBank, access number: AY278488) of the gB glycoprotein of HSV1 virus by computer, screen out the strong epitope (Fig. 1) in the gB glycoprotein of HSV1 virus, namely from From 1 amino acid to the 696th amino acid, the amino acid sequence is as follows:

[0218] Pro Ser Ser Pro Gly Thr Pro GIY Val Ala Ala Ala Thr Gln Ala Ala Asn Gly

[0219] Gly Pro Ala Thr Pro Ala Pro Pro Ala Leu GlY Ala Ala Pro Thr GlY Asp Pro

[0220] Lys Pro Lys Lys Asn Lys Lys Pro Lys Asn Pro Thr Pro Pro Arg Pro Ala Gly

[0221] Asp Asn Ala Thr Val Ala Ala Gly His Ala Thr Leu Arg Glu His Leu Arg Asp

[0222] Ile Lys Ala Glu Asn Thr Asp Ala Asn Phe Tyr Val Cys Pro Pro Pro Thr Gly

[0223] Ala Thr Val Val Gln Phe Glu Gln Pro Arg Arg Cys Pro Thr Arg Pro Glu Gly

[0224] Gln Asn Tyr Thr Glu Gly Ile Ala Val Val Phe Lys Glu Asn I...

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Abstract

The chemically synthesized HSV1 viral gB glycoprotein extracellular region gene fragment and its expression and application relate to the fields of genetic engineering technology, vaccines and diagnostic reagents. The present invention screens out the strong epitope in the gB glycoprotein of HSV1 virus through computer analysis, from the first amino acid to the 696th amino acid, a total of 696 amino acids, selects codons favored by both eukaryotic and prokaryotic organisms, and chemically synthesizes The brand-new gene sequence of the antigenic epitope uses genetic engineering technology to express the gene fragment and prepare a strong antigenic epitope fragment of the gB glycoprotein of the HSV1 virus. The expressed strong antigenic epitope fragment of gB glycoprotein of HSV1 virus can be used for the detection of vaccine, HSV1 virus antibody or antigen, and for immunization preparation of anti-HSV1 virus monoclonal antibody and polyclonal antibody and the like.

Description

technical field [0001] The present invention relates to a chemically synthesized brand-new gene fragment of the extracellular region of herpes simplex virus 1 (Herpes simplex virus 1, HSV1) glycoprotein B (glycoprotein B, gB glycoprotein), and uses genetic engineering technology to prepare recombinant HSV1 virus gB glycoprotein. Through computer analysis, the gB glycoprotein extracellular region fragment of HSV1 virus containing strong antigenic epitopes is screened out, the codons favored by both eukaryotic and prokaryotic organisms are selected, and a new gene sequence is chemically synthesized, and expressed by genetic engineering technology. The protein can be used for the detection of vaccines and HSV1 virus antibodies or antigens, etc. The invention relates to the fields of genetic engineering technology, vaccines and diagnostic reagents. Background technique [0002] Herpes simplex virus (HSV) is a common pathogen that endangers human health. It is easy to cause recu...

Claims

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Application Information

Patent Timeline
07 May 2008
Publication
CN101173290A
IPC
C12N15/38; C12N15/70; C07K14/035; C07K16/28; A61K48/00; G01N33/53; G01N33/569
Inventors
李袊希; 吕敏