The invention relates to a mixed refrigerant suitable for a 120-150K cryogenic temperature region of a single automatic cascading refrigeration system. The mixed refrigerant comprises the following six type refrigerants, by molar concentration, 3-20% of a first type refrigerant which is R740, R728 or a mixture of the R740 and the R728, 5-25% of a second type refrigerant which is R50, R784 or a mixture of the R50 and the R784, 15-30% of a third type refrigerant which is R14, 15-25% of a fourth type refrigerant which is R23, 5-15% of a fifth type refrigerant which is R125, R218, R22, R32 or a mixture of two, three or four of the R125, the R218, the R22 and the R32, and 20-30% of a sixth type refrigerant which is R236fa, R123, R124 or a mixture of two or three of the R236fa, the R123 and the R124. The corresponding mixed refrigerant is obtained through physical mixing at normal temperature, so the temperature of the system can reach 120-150K; and the mixed refrigerant has a good intersolubility with lubricating oil, so the ODP value is almost zero. The mixed refrigerant has the advantages of economy, environmental protection, safety, incombustibility and the like.