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Biodegradable collagen-based cornea substitute and preparation method thereof

A collagen-based cornea, biodegradable technology, applied in the field of collagen-based corneal substitutes and its preparation, can solve the problem of inability to take into account biocompatibility, biosafety, effectiveness of corneal transplantation, lack of clinical application value, and difficult marketization of products and other problems, to achieve excellent cell adhesion effect, good mechanical strength, and prevent molecular structure damage

Inactive Publication Date: 2019-01-04
SHAANXI HUIKANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the polymer MPDSAH and photoinitiator used in this method are stimulating reagents with poor biocompatibility, and chloroform solution is used for later storage, which is not as safe as the current terminal sterilization methods such as damp heat or radiation.
[0007] The artificial corneas disclosed in the above patents all have certain deficiencies, and cannot take into account biocompatibility, biosafety, and effectiveness after corneal transplantation. At the same time, some products are difficult to market and do not have clinical application value.

Method used

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  • Biodegradable collagen-based cornea substitute and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Mix 18 g of recombinant human collagen with a molecular weight of 60 kDa and 2 g of polyvinyl alcohol 124 with a number average molecular weight of 170,000 to obtain a collagen-based blend.

[0029] 2. Dilute the collagen-based blend obtained in step 1 to 100mL with deionized water, stir well, then add 0.02g of carbodiimide, and then put the resulting mixture in a mold for cross-linking reaction at 25°C 2h.

[0030] 3. Put the sample after the cross-linking reaction in step 2 into a 1kDa cut-off dialysis bag for cross-linking agent removal treatment, and replace the ultra-pure water in the external environment every 6 hours, a total of 4 times; the residual cross-linking agent will be removed in a clean environment Samples were placed in phosphate sterile preservation solution and packaged, treated at -10°C for 10h, 25kGy Co 60 Sterilized by irradiation to obtain biodegradable collagen-based corneal substitutes.

Embodiment 2

[0032] 1. Mix 8 g of recombinant human collagen with a molecular weight of 90 kDa and 8 g of polyvinyl alcohol 124 with a number average molecular weight of 120,000 to obtain a collagen-based blend.

[0033] 2. Dilute the collagen-based blend obtained in step 1 to 100mL with deionized water, stir well, then add 0.032g of transglutaminase, and then place the resulting mixture in a mold for cross-linking reaction at 4°C 48h; the obtained product was washed with PBS for 30min, then added to 100mL of deionized water, stirred evenly, added 0.016g of glutaraldehyde, and cross-linked at 25°C for 2h.

[0034] 3. Ultrasonic cleaning of the sample after the cross-linking reaction in step 2 to remove the cross-linking agent, and then place the sample in a clean environment in a phosphate sterile preservation solution and package it, and treat it at -10°C for 10 hours. 25kGy Co 60 Sterilized by irradiation to obtain biodegradable collagen-based corneal substitutes.

Embodiment 3

[0036] 1. Mix 8 g of recombinant human collagen freeze-dried powder with a molecular weight of 38 kDa, 1 g of polylactic acid with a number average molecular weight of 40,000, and 1 g of polyglycolic acid with a number average molecular weight of 20,000 to obtain a collagen-based blend.

[0037] 2. The above-mentioned collagen-based blend was formed into a scaffold by electrospinning technology to obtain a nanoscale three-dimensional mesh scaffold. The scaffold material was cut to the required size and immersed in 100mL ethanol solution containing 0.3g carbodiimide , cross-linking reaction at 25°C for 9h; the obtained product was rinsed with PBS for 30min, then immersed in 100mL aqueous solution containing 0.2g transglutaminase, and cross-linking reaction at 4°C for 16h.

[0038] 3. The corneal stent after the cross-linking reaction in step 2 was repeatedly washed with PBS to remove the remaining cross-linking agent, and after freeze-drying, it was packaged in a medical aluminu...

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Abstract

The invention discloses a biodegradable collagen-based cornea substitute and a preparation method thereof. The substitute is obtained as follows: collagen and a macromolecular polymer are subjected tocompound crosslinking treatment in a aqueous phase and then cured by a mol; or the collagen and the macromolecular polymer are subjected to electrospinning to form a nanofiber membrane, the nanofibermembrane is further subjected to compound crosslinking and drying to form a 3D cornea scaffold, and the 3D cornea scaffold is used after rehydration swelling clinically. The cornea substitute has controllable degradability, good phototropism and mechanism strength and significant induction functions on increase of corneal epithelial cells and corneal corpuscles.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering corneal graft materials, in particular, the invention relates to a collagen-based corneal substitute and a preparation method thereof. Background technique [0002] The blindness of more than 10 million people in the world is caused by corneal disease, which is the second leading cause of blindness after cataract. Penetrating keratoplasty is the main treatment for corneal diseases, but it has a poor prognosis for severe dry eye, Stevens-Johnson syndrome, severe chemical burns, etc., and faces problems such as graft rejection and insufficient donors. The application of artificial corneas and the research on tissue engineering corneas have brought hope to blind patients. At present, the commonly used corneal construction materials are mainly bio-scaffold materials and polymer materials. [0003] Biomaterial scaffolds often serve as mechanical scaffolds to support cell growth, mitigate ce...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/58D06M13/432D06M16/00D06M13/123D06M101/14D06M101/32D06M101/24
CPCA61L27/26A61L27/58A61L2400/12A61L2430/16D06M13/123D06M13/432D06M16/003D06M2101/14D06M2101/24D06M2101/32C08L89/00
Inventor 何越侯增淼王九娜高恩杨小琳赵金礼
Owner SHAANXI HUIKANG BIO TECH CO LTD
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