48 results about "Salmonella Gallinarum" patented technology

The invention discloses a veterinary drug for preventing livestock bowel diseases, a preparation method of the drug, and feed. The veterinary drug contains active ingredients which are prepared from purslane, humifuse euphorbia herb, plantain herb, acalypha australis and liquorice. The veterinary drug provided by the invention has the effects of clearing away heat and toxic materials, eliminating dampness to stop dysentery and astringing to stop bleeding, can be effectively used for preventing the livestock bowel diseases such as swine transmissible gastroenteritis, piglet diarrhea, swine dysentery, chicken salmonellosis, chicken colibacillosis and chicken stphyoococcl disease, and has the advantages of an extensive material source, safety, environment-friendliness, a simple preparation process, low production cost and the like.

A freeze-drying micro-ecological preparation for prevention and cure white diarrhea, which comprises lactobacillus acidophilus with a finite number of viable bacteria numbers, enterococcus (CMCC 1.130) and bacillus licheniformis (ATCC 250970).

The invention discloses a salmonella phage RDP-NSA-19050 with a wide lysis spectrum, wherein the preservation number is CGMCC No.21415, the gene length is 87591 bp; after sequencing is carried out on whole genes, it is found that the salmonella phage RDP-NSA-19050 does not have virulence genes and lyogenic genes, and the salmonella phage RDP-NSA-19050 has a good lysis spectrum. The salmonella bacteriophage RDP-NSA-19050 provided by the invention can be used for cracking a plurality of pathogenic bacteria such as salmonella steulani, salmonella gallinarum, salmonella typhimurium, salmonella paratyphi A, salmonella paratyphi B, salmonella typhimurium, salmonella enteritidis and salmonella kkinensis; the salmonella bacteriophage RDP-NSA-19050 has the characteristics of wide splitting spectrum and high splitting rate, can be independently used, can also be compounded with bacteriophages of other escherichia coli and salmonella and probiotics for use, and can effectively reduce the death rate of chicken infected with salmonella.

The invention relates to a fowl salmonella gallinarum attenuated isolated strain and application thereof. The isolated strain is preserved in the China General Microbiological Culture Collection Center, Beijing, China, on July 2, 2018, has a preservation number of CGMCC No.16049, and is classified and named as Salmonella Gallinarum. The strain is attenuated to chicken of different ages, is apathogenic, and can be used as a strain vector for preventing livestock salmonella gallinarum infection and preventing infectious diseases of livestock (chicken). The fowl salmonella gallinarum attenuated isolated strain provides data reference to evaluating the toxicity of salmonella attenuated vaccine strains and potential as the strain vector of livestock (chicken) live vaccines.

The invention discloses a salmonella pullorum SP9905. The strain is preserved at China Center for Type Culture Collection with an address of Wuhan University on October 15, 2014 and the preservation number is CCTCC NO.M 2014478. The invention also discloses an application of the salmonella pullorum in preparation of a live vaccine. The vaccine provided by using salmonella pullorum SP9905 has the advantages of high viable count and low freeze-drying death rate; the product is safe and reliable; a strong protecting force can be generated in the fourteenth day due to an immune protection effect, the immune protective rate is 100% and a good protective capability (80-100%) can be obtained for the other serotype salmonella (salmonella enteritidis and salmonella gallinarum).

The invention provides a pediococcus pentosaceus NHB-PpA9601. The pediococcus pentosaceus NHB-PpA9601 is separated from intestinal tracts of white feather broilers, and is preserved in China General Microbiological Culture Collection Center (CGMCC for short, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the postal code is 100101), the preservation number is CGMCC NO.22646, and the preservation date is May 31, 2021. The pediococcus pentosaceus NHB-PpA9601 has the advantages that the obvious probiotic property is realized, the growth and reproduction of pathogenic bacteria such as intestinal pathogenic escherichia coli, staphylococcus aureus, salmonella suis, salmonella choleraesuis, salmonella gallinarum, salmonella pullorum, salmonella typhimurium, salmonella, shigella, bacillus perfringens, proteus penneri, aeromonas hydrophila and vibrio parahaemolyticus can be effectively inhibited; the pediococcus pentosaceus NHB-PpA9601 can be used for producing a wet-based fermented feed, and can be used for effectively improving intestinal health and increasing economic benefits.

The invention discloses a para-cecropin antibacterial peptide and application thereof and belongs to the technical field of molecular biology. The para-cecropin antibacterial peptide consists of 31 amino acids and has the molecular weight of 3833.7Da. The antibacterial peptide can be used for inhibiting the growth of bacteria, such as staphylococcus aureus, avian pasteurella multocida, salmonella gallinarum and avian escherichia coli, has a better bacteriostasis effect compared with the commonly-used antibiotics, such as penicillin, streptomycin, cefazolin sodium and azithromycin, and has stronger bacteriostasis activity compared with the conventional cecropin antibacterial peptides, such as Cecropin B, Cecropin A and Cecropin P1. The antibacterial peptide has the minimum salmonella gallinarum bacteriostasis concentration of 625 micrograms per milliliter, has good thermal stability and has good application prospect in the research and development of antibacterial drugs.

The invention discloses specific novel molecular targets of 16 different serotypes of salmonella and a rapid detection method of the specific novel molecular targets. The invention provides a method for detecting salmonella enteritidis, which is used for different serotypes of salmonella. The invention method provides 72 specific molecular detection targets for different serotypes of salmonella including salmonella enteritidis, salmonella typhimurium, salmonella derby, salmonella indiana, salmonella agona, salmonella infantis, salmonella rissen, salmonella braenderup, salmonella virehow , salmonella albany, salmonella hadar, salmonella weltevreden, salmonella London, salmonella meleagridis, salmonella senftenberg and salmonella pomona, and corresponding PCR primer groups and a rapid detection method. The method provided by the invention has the advantages of short detection time, low cost, simple operation and strong specificity; meanwhile, the detection method can be used for detecting some strains with unexpressed antigens, avoiding the defects of immunodetection, and is more accurate in detection result, simpler in judgment and higher in practicability.

The invention relates to a pullorum disease agglutination antigen and a preparation method thereof and mainly relates to a bacterial strain which is salmonella pullorum standard strain C79-1 and a screened wild isolated strain S44. The wild isolated strain S44 of salmonella pullorum is classified and named as follows: salmonella enterica intestinal sub-salmonella pullorum, of which the Latin name is Salmonella enterica subsp. enterica pullorum, which is screened from the salmonella pullorum separated from chicken and which is collected at China General Microbiological Culture Collection Center with the collection No.: CGMCC No. 14258. The method provided by the invention is scientific and reasonable, stable in production and low in cost; the selected pullorum disease agglutination antigen production strain is high in antigenicity and low in aberration rate; and the prepared pullorum disease agglutination antigen product has the advantages of high sensibility, high specificity, quick diagnosis, easiness in observing agglutination effect, and the like.

The invention discloses primers, probes, a test kit and a test method for triple real-time fluorescence PCR detection of Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum. According to the present invention, a triple real-time PCR method is used, wherein a consensus sequence of Salmonella choleraesuis and Salmonella paratyphi is used for the detection of Salmonella choleraesuis and Salmonella paratyphi, a specific sequence of Salmonella typhi is used for the detection of Salmonella typhi, and a specific sequence of Salmonella gallinarum is used for the detection of Salmonella gallinarum. Whether a sample is contaminated by Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum is determined via a real-time fluorescence PCR amplification, and then Salmonella choleraesuis and Salmonella paratyphi are distinguished via a single fluorescent PCR amplification; the detection is rapid, the process of preparing sample and issuing test results can be finished in 31hours, the results are reliable, and sensitivity and specificity are high.

The invention provides a Chinese medicinal composition for treating drug-resistant Salmonellosis avium, and a preparation method of the Chinese medicinal composition. The composition is prepared from (by weight parts) Rhizoma Coptidis 2-8, Radix Scutellariae 8-12, Radix Pulsatillae 1-6, Flos Chrysanthemi Indici 1-5, Pericarpium Granati 1-5, Galla Chinensis 1-5, and Radix Glycyrrhizae 2-10 by ultra-micronizing and mixing. The composition for treating drug-resistant Salmonellosis avium has the advantages of reasonable formula, synergic effect of Chinese traditional medicine components, definite effect in treating Salmonellosis avium, simple preparation method, and suitability for mass industrial production, and has antibacterial, anti-inflammatory and immunity-enhancing functions.

The invention provides a composition for treating fowl salmonellosis, and the active ingredients are composed of the following raw materials: golden thread, dried rehmannia root, baical skullcap root, common selfheal fruit-spike, medicinal fermented mass, malt, liquoric root and gypsum. The gypsum, the malt and the liquoric root are respectively smashed into coarse powder for standby; then the residual medicines are decocted together, the decoction is continuously carried out for 3 times with 1-2 hours for each time, and decocted liquid of the three times is merged after the decoction and concentrated to be thick; the coarse powder of the malt and the liquoric root is mixed in the thick liquid and evenly stirred; the gypsum powder is mixed after being dried and smashed, thereby preparing powder or tablets. The pharmaceutical composition can effectively treat bacterial and viral infectious diseases, in particular to the fowl salmonellosis, the cure rate is higher than 85 percent, and the pharmaceutical composition has difficult relapse, obvious effects, convenient administration, no toxicity or side effects, safe use and low price. Bacteria have difficulty in generating the drug resistance by using the drug, thereby avoiding the occurrence of veterinary drug residues and social public harms.

The invention discloses a traditional Chinese medicine feed for preventing and treating avian salmonellosis and a preparation method of the traditional Chinese medicine feed. The traditional Chinese medicine feed for preventing and treating avian salmonellosis is prepared from the following raw materials in parts by weight: corn flour 80-100 parts, bean pulp 80-100 parts, wheat bran 80-100 parts, mulberry leaf 10-20 parts, bone meal 8-12 parts, lactobacillus plantarum 2-5 parts, saccharomyces cerevisiae 1-3 parts and a traditional Chinese medicine additive 1-2 parts. The traditional Chinese medicine additive is prepared from the following raw materials in parts by weight: 22-30 parts of Radix Et Rhizoma Rhei, 22-30 parts of Radix Angelicae Dahuricae, 20-25 parts of Herba Portulacae, 15-20 parts of Rhizoma Dioscoreae, 15-20 parts of Endothelium Corneum Gigeriae Galli, 15-20 parts of Fructus Lycii, 15-20 parts of Radix Codonopsis and 10-15 parts of Radix Glycyrrhizae. The cure rate of the traditional Chinese medicine feed to the avian salmonellosis is high, and after the avian salmonellosis is prevented and treated, the food consumption and the laying rate of chicken flocks can recover to the normal level.

The invention discloses a non-diagnostic purpose method for rapidly detecting Salmonella pullorum and Salmonella gallinarum. The detection method comprises the following steps: preparing Salmonella pullorum and Salmonella gallinarum antibody labeled coupled magnetic nano-particles (IgG-MNPs) and antibody and enzyme double labeled silica nano-particles (GOx-IgG-SiNPs), forming an immune compound IgG-MNPs/S having a sandwich structure, S. Pullorum and S. Gallinarum GOx-IgG-SiNPs, when the target bacteria to be detected exist, performing an enzymatically catalyzed reaction, allowing the obtainedreaction product and a color developer to form a complex product, and detecting the absorbance value of the obtained system to obtain the concentrations of the Salmonella pullorum and Salmonella gallinarum in order to realize the quantitative detection of the Salmonella pullorum and Salmonella gallinarum. A cascade reaction triggered by glucose oxidase (GOx) is combined with an Fe<3+>-SCN<-> colordevelopment system to realize the quantitative detection of Salmonella pullorum and Salmonella typhimurium. The detection method has the advantages of convenience, fastness, easiness in operation, good sensitivity, good specificity and good stability.

The invention discloses a primer group, a kit and a method for identifying salmonella pullorum and salmonella gallinarum. The primer group comprises two pairs of specific primers, wherein the two pairs of specific primers are respectively primer 1 and primer 2 as well as primer 3 and primer 4, wherein the primer 1 is as shown in SEQ ID NO: 1, the primer 2 is as shown in SEQ ID NO: 2, the primer 3 is as shown in SEQ ID NO: 3 and the primer 4 is as shown in SEQ ID NO: 4; the kit contains the primer group; and the primer group is used for detecting to-be-detected bacteria by virtue of double PCR amplification. The primer group for identifying salmonella pullorum and salmonella gallinarum disclosed by the invention is strong in detection reaction specificity; through a PCR reaction, the salmonella pullorum and the salmonella gallinarum can be simultaneously detected from a sample, with high detection reaction sensitivity; and a detection process is rapid and efficient, and suitable for mass detection.

A preparation method of a radix isatidis oral solution comprises the following steps: carrying out superfine grinding on radix isatidis into reach 500 meshes until plant cell walls of radix isatidis are completely damaged; soaking radix isatidis superfine powder into injection water at a room temperature for 2 hours, carrying out preparation processes of filtration, centrifugation, chitosan impurity removal and the like so as to extract active components of the radix isatidis superfine powder, carrying out reduced pressure concentration so as to prepare transparent liquid with a raw radix isatidis content of 1.0g/ml, heating the transparent liquid at 63 DEG C for 30 minutes for sterilization, and respectively packaging the transparent liquid into 250ml brown bottles. The preparation method is characterized in that a traditional method for preparing oral solutions by decocting traditional Chinese medicines is changed, and the method is a brand-new technique for preparing traditional Chinese medicine oral solutions. The radix isatidis oral solution is drunk with water in use. When the radix isatidis oral solution is used for preventing salmonellosis of chicks, each chick is fed with 0.25g of the oral solution, namely 250ml of the oral solution with a raw medicine concentration of 1.0g/ml can be blended with water so as to feed 1000 chicks once a day; and when the radix isatidis oral solution is used for preventing salmonellosis of chickens, each chicken is fed with 0.5g of the oral solution, namely 250ml of the oral solution with a raw medicine concentration of 1.0g/ml can be blended with water so as to feed 500 chickens once a day.

The invention belongs to the technical field of mutation or genetic engineering, discloses a method using Bi-PASA markers of three defensin genes to improve resistance ability of chicken salmonella, aprimer and a kit, and provides bidirectional allele specific PCR (polymerase chain reaction) (Bi-PASA) genetic markers of three defensin genes. The method is characterized in that the Bi-PASA technique is adopted for the first time; on the basis of analysis of susceptibility of chicken salmonellosis correlated with the variance of the three defensin genes, after the SNP (single nucleotide polymorphism) site and gene haplotype genetic variance are analyzed, the three defensin genes as function genes for resisting the chicken salmonellosis are disclosed, the simple, time-shortening, low-cost, quick and effective Bi-PASA detection marker for the three defensin genes is established, and the positive guidance meaning is realized on the gene purification and disease-resistant breeding. The three defensin genes are used for the gene detection of resistance ability of chicken salmonellosis and the molecular marker-assisted breeding, and have the characteristics of early screening, quick identification, time shortening, low cost, high accuracy and the like.

The invention relates to a method for purifying salmonella of laying hens. The purification method comprises the following steps that firstly, stock progenitor chickens are selected, and all antibody positive chickens are weeded out; secondly, parent chickens are bred, infectious disease sources are prevented in the breeding process, a conventional chicken salmonella vaccine is used for water drinking, finally, the bred parent chickens are detected, and all the antibody positive chickens are weeded out; thirdly, a chicken house needs to be thoroughly cleaned before hatching of commercial chickens, and the hatching environment needs to be disinfected strictly in the hatching process, wherein the hatching environment is disinfected once a week under the normal condition, disinfected twice a week in the case of diseases and not disinfected three days before and after immunization. The method has the advantages that the disease source of the salmonella of the laying hens can be effectively purified, and the purification degree of the laying hens can be greatly improved.

The invention belongs to the field of animal husbandry and veterinary and in particular relates to a method for simultaneously purifying and detecting avian leukosis and avian salmonellosis by utilizing one hatching egg and application of the method. The method comprises the following steps: feeding breeder flocks in single cages and encoding; collecting primary hatching eggs of the breeder flocks; enabling the primary hatching eggs to correspond to numbers of the hatching eggs one by one; disinfecting the surfaces of the hatching eggs and opening egg shells from hatching egg air chamber ends;sucking egg white and yolks and putting into PE (Polyethylene) tubes respectively and marking; putting egg white and yolk samples into a refrigerator and freezing and thawing for a plurality of times; taking the frozen and thawed egg white; detecting an ALV virus antigen through an avian leukosis ELISA (Enzyme Linked Immunosorbent Assay) detection method; taking the frozen and thawed yolks and adding normal saline and chloroform; after uniformly mixing on an oscillator, centrifuging and layering a solution, wherein a lower layer is an organic solvent, a middle layer is denatured lipid proteinand an upper layer is an extracted antibody; and sucking the antibody of the upper layer and detecting a salmonellosis antibody through a plate agglutination method; and eliminating chicken flock individuals with positive detection results of the ALV virus antigen and the salmonellosis antibody.