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A kind of Salmonella pullorum sp9905 and its application

A technology of Salmonella and pullorum, applied in the field of bioengineering, can solve the problems of increased mortality, abuse or waste, selection of drug types, administration routes, methods, dosage and maintenance time confusion, etc., and achieve low freeze-drying mortality , high bacterial count, product safety and reliability

Active Publication Date: 2018-02-02
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pullorum is ubiquitous in chicken farms in my country, manifested in increased mortality in the brooding and rearing stages, causing serious economic losses to producers
Domestic control of pullorum pullorum usually uses antibacterial drugs to reduce its harm, but the selection of drug types, routes of administration, methods, doses and maintenance time is relatively confusing, there is a considerable degree of blindness, and abuse or waste is very common
At the same time, long-term repeated medication will not only increase the cost of feeding, but also lead to a series of problems such as enhanced bacterial resistance and drug residues in poultry products.
Facts have proved that only relying on drug control is not a good way to prevent pullorum

Method used

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  • A kind of Salmonella pullorum sp9905 and its application
  • A kind of Salmonella pullorum sp9905 and its application
  • A kind of Salmonella pullorum sp9905 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0019] 1) Separation, purification and staining microscopy

[0020] Salmonella pullorum SP9905 strain was isolated from the cecum contents of 11-month-old Langshan chickens in a chicken farm in Yangzhou City, Jiangsu Province in May 1999. The specific separation method is as follows: aseptically collect cecal contents, add them to selenite cystine enrichment solution for overnight culture at 37°C, then inoculate MacConkey agar at 37°C for 24 hours, pick out 0.5mm-1.0mm in diameter, Off-white transparent colonies with smooth surface, inoculated on Martin agar medium, cultured at 37°C for 24 hours. The purely cultured bacteria were subjected to Gram staining microscope inspection, and then observed with an optical microscope, showing elongated bacilli with slightly rounded ends.

[0021] 2) Biochemical identification

[0022] Colonies were picked from Martin agar plates for biochemical identification tests. Trace biochemical identification tubes were purchased from Hangzhou M...

Embodiment 2

[0040] After the rejuvenation of Salmonella pullorum SP9905 strain, typical colonies were selected for expansion and culture to make seed bacterial liquid. Inoculate the seed bacterial liquid into the liquid medium according to 2.5% of the total amount of the medium, collect the bacterial liquid after 20 hours of deep aeration culture at 37°C, centrifuge at 4000r / min for 15 minutes to remove the supernatant, and immediately add the bacterial sludge to the sterilized freeze-drying protective agent Mix well, subpackage and vacuum freeze-dry to make Salmonella pullorum live vaccine. The test results showed that each milliliter of culture medium contained 44.876 billion live bacteria, and the freeze-drying mortality rate was 14.9%.

Embodiment 3

[0042] After the rejuvenation of Salmonella pullorum SP9905 strain, typical colonies were selected for expansion and culture to make seed bacterial liquid. Inoculate the seed bacteria solution into the liquid medium according to 5% of the total amount of the medium, collect the bacteria solution after 18 hours of deep aeration culture at 37°C, centrifuge at 4000r / min for 15 minutes to remove the supernatant, and immediately add the bacteria slime to the sterilized freeze-drying protective agent Mix well, subpackage and vacuum freeze-dry to make Salmonella pullorum live vaccine. The test results showed that each milliliter of culture medium contained 51.743 billion viable bacteria, and the freeze-drying mortality rate was 22.5%.

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Abstract

The invention discloses Salmonella pullorum sp9905, which has been preserved. The preservation unit: China Typical Culture Collection Center; address: Wuhan University; preservation date: October 15, 2014; collection number: CCTCC NO.M 2014478 . The invention also provides the application of the Salmonella pullorum pullorum in the preparation of live vaccines. The vaccine prepared by the present invention has the advantages of high number of viable bacteria and low freeze-drying mortality rate, and the product is safe and reliable. The immune protection can produce a strong protective force on the 14th day, and the immune protection rate reaches 100%. It is also resistant to other serotypes of Salmonella (Salmonella enteritidis, Salmonella gallinarum typhi) also has a good protection ability (80%~100%).

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Salmonella pullorum sp9905, and its application and preparation method in the preparation of live vaccines. Background technique [0002] Pullorum pullorum is a common multiple infectious disease of poultry caused by Salmonella pullorum. Pullorum is ubiquitous in chicken farms in my country, and it shows that the mortality rate increases in the brooding and rearing stages, causing serious economic losses to producers. Domestic control of pullorum pullorum usually uses antibacterial drugs to reduce its harm, but the selection of drug types, routes of administration, methods, dosage and maintenance time is relatively confusing, there is a considerable degree of blindness, and abuse or waste is very common. At the same time, long-term repeated medication not only increases the cost of feeding, but also leads to a series of problems such as enhanced bacterial re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K39/112A61P31/04A61P1/12C12R1/42
Inventor 龚建森苗晋锋赵旭炊慧霞张笛乐敏张萍韩先干窦新红刘学贤
Owner JIANGSU INST OF POULTRY SCI
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