The invention discloses a selenium-rich cookie containing kudzuvine roots and lentinus edodes and a preparation method. The cookie comprises following components in a certain proportion: L-selenium-methylselenocysteine, active dry yeast powder, kudzuvine root powder, lentinus edodes stem powder and flour. The preparation method comprises the steps as follows: 1), taking kudzuvine roots and lentinus edodes for washing and smashing; 2), weighing raw materials, vegetable oil, butter, eggs, xylitol and sodium bicarbonate; 3), uniformly mixing the weighed kudzuvine root powder and lentinus edodes stem powder, and spraying the mixture by a cellulose solution into mixed powder for enzymolysis; 4), adding the active dry yeast powder and the flour into the kudzuvine root powder and lentinus edodes stem powder after enzymolysis, and performing fermentation; and 5), uniformly mixing the weighed L-selenium-methylselenocysteine, vegetable oil, butter, eggs, xylitol and sodium bicarbonate with the fermented kudzuvine root powder, lentinus edodes stem powder and flour for tablet modeling, and obtaining the selenium-rich cookie containing kudzuvine roots and lentinus edodes. According to the invention, the formula is reasonable, the three substances are mutually matched, selenium, puerarin and lentinan are adopted to improve the efficacy of a product, and the immune efficacy is enhanced.