Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof
A feed additive, yew technology, applied in application, animal feed, animal feed, etc., to achieve the effect of improving absorption capacity, promoting production efficiency, and mild treatment
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Embodiment 1
[0033] The preparation of embodiment 1 fermented liquid
[0034] This example only takes Lactobacillus plantarum (ACCC11016), Lactobacillus acidophilus (ACCC03955), and Candida utilis (ACCC20060) as examples to describe the specific content of the invention in detail, and does not limit the three components of the compound microbial agent of the present invention. selection of microbial sources.
[0035] (1) Activate the above-mentioned Lactobacillus plantarum (ACCC11016), Lactobacillus acidophilus (ACCC03955), and Candida utilis (ACCC20060) into their respective slant media for preservation, and culture them at 28-30°C for 24-48 hours.
[0036] Preservation of three kinds of microorganisms uses the slant culture medium formula as follows:
[0037] Both Lactobacillus plantarum and Lactobacillus acidophilus use MRS medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast extract, 5.0g glucose, 5.0g sodium acetate, 2.0g diammonium citrate, Tween801.0 Gram, K 2 HPO 4 2.0 ...
Embodiment 2
[0043] Embodiment 2 contains the poultry feed of fermented yew poultry feed additive of the present invention
[0044] 1. The three kinds of microbial fermented liquids prepared in Example 1 are mixed uniformly according to the following proportions to become a composite microbial bacterial agent: 8L of Lactobacillus plantarum fermented liquid, 10L of Lactobacillus acidophilus fermented liquid, 12L of Candida utilis fermented liquid, after mixing Add distilled water to 50L. Among them, the effective number of viable bacteria of the three kinds of bacteria is not less than 5×10 7 individual / ml.
[0045] 2. Select 10kg of yew branches and leaves powder that has been crushed to 50 meshes and add it to the strain reaction tank, and measure its cellulose content to be 38.2g / g dry powder;
[0046] 3. Add 50L of the compound microbial agent prepared in step 1 to the reaction tank, add 500g of brown sugar and 750g of peptone at the same time, after mixing evenly, measure the polysac...
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