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Application of bacillus velezensis to degradation of cellulose in tobacco flavor raw materials

A bacillus and cellulose technology, applied in the fields of application, tobacco, and tobacco treatment, can solve the problems of cigarette quality side effects, bad smell, etc., and achieve the effects of reducing cellulose content, improving aroma quality, and high cellulose degradation rate

Inactive Publication Date: 2020-12-08
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Tobacco incense raw materials are mostly plant roots, stems, seeds, etc. These raw materials are rich in cellulose, which has a direct impact on the aroma of tobacco incense raw materials, and usually produces a A sharp stimulation and a bad smell similar to burning paper, which have a negative effect on the quality of cigarettes

Method used

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  • Application of bacillus velezensis to degradation of cellulose in tobacco flavor raw materials
  • Application of bacillus velezensis to degradation of cellulose in tobacco flavor raw materials
  • Application of bacillus velezensis to degradation of cellulose in tobacco flavor raw materials

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The application of Bacillus Velez in degrading cellulose in citronella grass comprises the following steps:

[0025] (1) Dissolve 10 grams of tryptone, 5 grams of yeast extract, and 5 grams of sodium chloride in 1000 mL of deionized water, and sterilize at 121 °C for 20 minutes under high temperature and high pressure to prepare LB liquid medium.

[0026] Use the inoculation loop to pick the preserved strain of Bacillus velezensis B-3 cultured on the slant medium (the formula is the same as that of LB liquid medium, only add 15g / L agar powder), and place it in the LB liquid medium , Suspension culture at 30°C for 12h for strain activation. Then transfer the bacterial solution to LB liquid culture medium, and culture it on a shaker at 35°C until OD 600 =3.5, it can be used as bacterial agent for degrading citronella grass after being evenly suspended.

[0027] (2) Cut the same batch of citronella grass into 1cm segments and divide it into two parts of equal mass. One p...

Embodiment 2

[0032] The application of Bacillus Velez in degrading cellulose in figs comprises the following steps:

[0033] (1) Dissolve 10 grams of tryptone, 5 grams of yeast extract, and 5 grams of sodium chloride in 1000 mL of deionized water, and sterilize at 121 °C for 20 minutes under high temperature and high pressure to prepare LB liquid medium.

[0034] Use the inoculation loop to pick the preserved strain of Bacillus velezensis B-3 cultured on the slant medium (the formula is the same as that of LB liquid medium, only add 15g / L agar powder), and place it in the LB liquid medium , Suspension culture at 30°C for 12h for strain activation. Then transfer the bacterial solution to LB liquid culture medium, and culture it on a shaker at 35°C until OD 600 =1.0, it can be used as a bacterial agent for degrading figs after being evenly suspended.

[0035] (2) Cut the same batch of figs into small pieces with a thickness of 1 cm and divide them into two parts of equal mass. One part is ...

Embodiment 3

[0040]The application of Bacillus Velez in degrading cellulose in stevia (a kind of chrysanthemum) comprises the following steps:

[0041] (1) Dissolve 10 grams of tryptone, 5 grams of yeast extract, and 5 grams of sodium chloride in 1000 mL of deionized water, and sterilize at 121 °C for 20 minutes under high temperature and high pressure to prepare LB liquid medium.

[0042] Use the inoculation loop to pick the preserved strain of Bacillus velezensis B-3 cultured on the slant medium (the formula is the same as that of LB liquid medium, only add 15g / L agar powder), and place it in the LB liquid medium , Suspension culture at 30°C for 12h for strain activation. Then transfer the bacterial solution to LB liquid culture medium, and culture it on a shaker at 35°C until OD 600 =5.0, it can be used as bacterial agent for degrading stevia after being uniformly suspended.

[0043] (2) Cut the same batch of stevia into 1cm segments and divide it into two parts of equal mass. One par...

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Abstract

The invention relates to the technical field of treatment of tobacco flavor raw materials, in particular to application of bacillus velezensis to degradation of cellulose in the tobacco flavor raw materials. The bacillus velezensis is a bacillus velezensis B-3 strain, and is preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus velezensis is CCTCC No. M2019820. According to the application of the bacillus velezensis, through selecting the specific bacillus velezensis strain and amplifying the bacillus velezensis strain to prepare the microbial inoculum, the microbial inoculum is applied to the tobacco flavor raw materials, so that the cellulose content in the tobacco flavor raw materials is effectively reduced, and the flavor quality of thetobacco flavor raw material is improved.

Description

technical field [0001] The invention relates to the technical field of processing tobacco aroma raw materials, in particular to the application of Bacillus velei in degrading cellulose in tobacco aroma raw materials. Background technique [0002] Tobacco incense raw materials are mostly plant roots, stems, seeds, etc. These raw materials are rich in cellulose, which has a direct impact on the aroma of tobacco incense raw materials, and usually produces a A sharp stimulation and a bad smell similar to burning paper have adverse effects on the quality of cigarettes. [0003] Due to the relatively stable structure of cellulose, it is difficult to completely separate it during the extraction and separation of flavor raw materials. In view of this defect, enzyme preparations and biological preparations are developed and used to accelerate the degradation of cellulose macromolecular substances in tobacco flavor raw materials and improve It is of great significance to improve the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/28
CPCA24B15/28
Inventor 董爱君叶明樵肜霖潘曦李冉
Owner HUBEI CHINA TOBACCO IND
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