328 results about "Taxus" patented technology

The invention discloses a nutrient solution cultivation method of Chinese yews and belongs to the method of plant nutrient solution cultivation. The method includes the steps: A, plant treatment: cutting off fibril and reserving main roots to be soaked in carbendazim and naphthylacetic acid; B, planting: planting the Chinese yews inside a planting basket with ceramicite; C, root speeding: placing the Chinese yews inside perlite matrix and enabling intelligentized laminas to be buried inside the perlite matrix, and controlling micro-environment factors to regulate and control a system through a plant non-test-tube rapid propagation expert system; D, root promoting: when matrix roots penetrate out the planting basket, enabling the Chinese yews to be soaked into a nutrient solution water tank and arranging the intelligentized laminas, and controlling the micro-environment factors to regulate and control the system through the plant non-test-tube rapid propagation expert system; E, cultivating to be strong, arranging the intelligentized laminas in the positions of hydrophilous root systems and plant laminas, and controlling the micro-environment factors through the plant non-test-tube rapid propagation expert system; F, modeling: trimming and modeling the Chinese yews into commodity bonsai. By the adoption of the method, breeding time is reduced by 60-80%, the new manual cultivation method of the Chinese yews is initiated, and the new way of utilizing the Chinese yews is expanded.

A method for planting enqlish yew includes collecting and treating seeds, preparing soil, sowing, seedling-phase, management, creating seedling nursery, cutting, rooting and cuttage.

The invention discloses a method for extracting and separating taxol and other taxane compounds from yew. The method includes the steps of extraction, degreasing, crude separation to an organic phase and an aqueous phase respectively, refined separation with reverse-phase chromatography, and crystallization. The invention sequentially adopts the ethanol of 80 to 95 percent, 60 to 75 percent and 20 to 55 percent to carry out gradient extraction to the yew material and also to fully extract taxane compounds with different polarities so as to lead the total extraction rate of the taxane compounds to be raised to more than 90 percent from 70 percent of the traditional art; the simultaneous extracting of 8 different tazane compound monomers from one set of the yew material is realized by the invention and the purity of each monomer can reach more than 98 percent; an aqueous phase substance which is generated in the extraction process abandoned by the traditional art is fully utilized to raise the utilization rate of the taxane compounds in the yew by 10 to 20 times, therefore, the bottleneck problem of industrial production of the lack of yew material is effectively solved.

The invention relates to a method for extracting dietary fiber from Chinese yew. The method disclosed by the invention adopts a high pressure homogenization-enzyme combination method. According to the extraction method of the dietary fiber, disclosed by the invention, the yield of the soluble dietary fiber can be improved, the production process is simple and easy to operate, and chemical residues can be avoided.

The invention discloses a three-dimensional interplanting method of Chinese yew, conifer and fern. The method includes the steps of soil preparation, transplantation and interplantation, field management and the like. Particularly, the method includes selecting neutral to acidic medium-layer thick soil at an altitude of 1600-2500m, digging seedling beds and applying base fertilizer; and after the Chinese yew is lifted, submerging the seedling root of the Chinese yew into mud mixed with organic fertilizer, and then interplanting the Chinese yew into a three-dimensional growing environment in which the upper-layer vegetation is the conifer and the ground vegetation is the fern, wherein two rows of the Chinese yew are interplanted between every two rows of the conifer, and a row of the fern is interplanted between every two rows of the Chinese yew. The row distance of the Chinese yew is 80-90cm, the inter-plant distance of the Chinese jew is 50-60cm, the row distance between the Chinese row and the conifer is 100-120cm, and the inter-plant distance of the conifer is 160-180cm. After the Chinese yew is transplanted and interplanted, the weeding and earthing-up should be implemented 3-5 times a year, the topdressing should be implemented 2-3 times a year, and the topdressing of the conifer seedlings should be implemented once a year. Experiments prove that according to the three-dimensional interplanting method of the Chinese yew, the conifer and the fern, the survival rate, the growth rate and the setting rate of the Chinese yew are improved greatly, and meanwhile, fast growth, early fruit bearing and capability of large-area popularization and plantation can be obtained.

The invention discloses a Chinese yew vegetative propagation method which comprises the following steps: (1), planting and preparing a cutting bed; (2), cutting Chinese yews; (3), managing a nursery bed. After the adoption of the method provided by the invention, plant growing is good, the survival rate is high, and the method is conducive to vegetative propagation of Chinese yews.

The invention discloses a special culture medium for culturing yew plant cells to produce taxol, which is obtained by taking B5 culture medium as a basic formula and utilizing an orthogonal optimization test. When the basic component comparison production is carried out on the special culture medium and the B5 culture medium, the content of the taxol in the special culture medium is 4.2 times that of the B5 culture medium; when high-density of yew plant cells are planted, a combined test is carried out by adding silver nitrate, salicylic acid, chitosan, methyl jasmonic acid, tribasic ammonium citrate, lanthanum sulfate, phenylalanine, glycine, tryptophan and lysine in the culturing process, which can further improve the content of the taxol and shorten the production period of cells; and when the cells are cultured at the thirty-first day, the highest content of the taxol in the special culture medium is 285.15 mg/L which is 81 times the highest content of the taxol in the B5 culture medium, thus the taxol can be effectively produced by using the special culture medium for culturing yew plant cells to produce taxol.

The present invention relates to a composition for preventing or treating cancer, which contains, as an active ingredient, a Taxus cambium- or procambium-derived cell line; a lysate thereof; an extract thereof; or a culture medium thereof. The cell line, the lysate, the extract, and the culture medium has minimized side effects compared to the conventional therapeutic drugs, is safe to the human body, is involved directly in the growth of cancer to induce cancer cell death, and shows anticancer activity of inhibiting angiogenesis occurring in carcinogenesis. Accordingly, the cell line, the lysate, the extract and the culture medium is useful for the prevention, treatment and alleviation of cancer.

The invention relates to a Chinese yew aeroponic method. The Chinese yew aeroponic method comprises the steps of erecting a cultivation rack with a trapezoid cross section and laying planting plates on two side oblique planes of the cultivation rack; opening a spraying device and using electricity functional water and clear water to perform preliminary disinfection and cleaning on the inside and outside of the cultivation rack before field planting; selecting rapid propagating Chinese yew seedlings growing well, free of diseases and insect pests and provided with developed roots, arranging and trimming the root tip positions of root systems, planting the roots of the rapid propagating Chinese yew seedlings into planting holes of the cultivation rack, and using sponge blocks to plant Chinese yews into the planting holes; directly spraying a nutrient solution and water to the roots of the Chinese yews in an aeroponic mode to perform cultivation after field planting; shearing root hair after the root hair grow to meet enough length requirement. By means of the Chinese yew aeroponic method, the root hair is not planted in soil, is convenient to shear and can be repeatedly bred and sheared.

The invention relates to a cutting propagation method for Chinese yew by seedling pots. The method includes the steps of preparation of nutrient substrate, preparation of seedling pots, selection of shoots, pre-cuttage treatment, cuttage in fields, post-cuttage sterilization, temperature and humidity holding, subsequent management and the like. The cutting propagation method for Chinese yew by seedling pots facilitates post-cuttage transplanting or pot replacement for Chinese yew, is convenient and enables survival rate of Chinese yew to be higher.

The invention discloses taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens, which is classified and named as pseudomonas fluorescens CLW17 collected in China Center for Type Culture Collection with the collection number of CCTCC NO. M 2010158. The invention further discloses an application of the taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens in promoting the growth of taxus chinensis var. mairei. A strain of the invention has very strong effect of dissolving insoluble phosphates of tricalcium phosphate, iron phosphate, aluminum phosphate, Jiangsu Jinping phosphate rock powder and hydroxylapatite under the situation of being cultured in a liquid shake flask; and the strain is produced into a microbial agent to be respectively inoculated in a cutting seedling and a seedling of the taxus chinensis var. mairei, and the results show that the microbial agent can significantly promote the growth and the development of the two. Therefore, the invention can provide excellent strain resources for special microbial fertilizers for developing the taxus chinensis var. mairei in the future.

A process for primarily extracting 10-deacetyl palcatine C from the twig and leaf of enqlish yew includes extracting in alcohol solution, concentrating laying aside, deposing taxol and low-polarity impurities, filtering, regulating pH=5-6, macroreticular resin adsorbing, eluting with absolute alcohol, and concentrating.

The invention discloses efficient phosphate-solubilizing penicillium pinophilum of laxus malrel rhizosphere. The classified name of the efficient phosphate-solubilizing penicillium pinophilum of laxus malrel rhizosphere is penicillium pinophilum CBW10, and the penicillium pinophilum is collected in the CCTCC (China Center for Type Culture Collection) and has the collection number of CCTCC No.M2011136. The invention also discloses application of the efficient phosphate-solubilizing penicillium pinophilum of laxus malrel rhizosphere to accelerating the growth of the laxus malrel. The bacterial strain disclosed by the invention has stronger dissolving effects on difficultly-soluble phosphates, such as tricalcium phosphate, ferric phosphate, aluminum phosphate, Jinping powdered rock phosphate in Jiangsu and hydroxyapatite; and the bacterial strain is prepared into microbial inoculums which are respectively inoculated into cuttage seedlings and seedling plants of the laxus malrel and the result shows that the bacterial strain can obviously accelerate the growth and development of the cuttage seedlings and seedling plants of the laxus malrel. Therefore, according to the invention, good bacterial strain resources can be supplied to develop a special microorganism fertilizer of the laxus malrel in the future.

The invention relates to a method for highly efficiently extracting paclitaxel from Chinese yew. According to the invention, a Chinese yew raw material is ground and sieved using a 100-200 mesh sieve; water with an amount 5-10 times that of ground Chinese yew and 0.1% glacial acetic acid are added, and ultrasonic-assisted enzymolysis is carried out; the obtained material is allowed to stand, and is filtered and separated; ultrasonic-assisted organic solvent extraction is carried out; impurity-removing is carried out through column chromatography; alkaline resin column continuous purification and separation is carried out; and recrystallization is carried out, such that a pure product with a purity higher than 99.3% is obtained. The method provided by the invention has the advantages of simple process steps, low cost, good repeatability, and high product purity.

The invention provides a technology for generating paclitaxel from Chinese yew cells. In the technology, Chinese yew callus cells are produced in large-scale in a mode of combing light culture and dark culture. The invention is characterized in that callus induction is carried out on Chinese yew on a B5 improved culture medium to induce the Chinese yew cells which do not have brown stain and can be continuously subcultured; the light culture and the dark culture are combined to be used for culturing the Chinese yew cells to obtain high yield cells with paclitaxel the content of which is above 0.12 %; and the Chinese yew cells are subjected to subculturing multiplication so that a great amount of Chinese yew cells are produced. The cell culture can be directly used for extracting the paclitaxel, thereby overcoming the defects of deficient resources, low content, damage to ecological environment and the like of the process for producing the paclitaxel from plant bark. Compared with the existing technology for producing the paclitaxel through the cell culture, the technology of the invention has the characteristics that the content of the paclitaxel can be stably improved without degradation and the operation is simple and convenient.

The invention relates to the technical field of agriculture, in particular to a method for breeding Chinese yew seeds. The method comprises the steps of selection of a seedling raising field, sorting of a seed bed, collection and treatment of seeds, pre-sowing treatment and sowing of seeds, field management and transplanting, young growth tending, planting and breeding. Due to the selection of the seeding raising field and sorting of the seed bed, the defect of severe field selection existing in the conventional Chinese yew seed planting method is overcome; through the collection and treatment processes of seeds, the dormant period of seeds is shortened, the pre-hull-breaking treatment difficulty before sowing is lowered, and the breeding period of the Chinese yew seeds is further shortened; and through treatment before sowing as well as field management and the young growth tending process after sowing, so that the survival rate and outplanting rate of Chinese yew are increased, basic conditions are provided for the production of large-scale production of Chinese yew, and the medicinal, material and ornamental values of Chinese yew are brought into full play.

The invention relates to an extraction method of a taxus active extract. According to the invention, taxus branches and leaves are crushed to 20-50 meshes; petroleum ether with a 3-5-time amount is added; the mixture is placed under 0-5 DEG C for 10-12h; filtering is carried out, such that a filter slag is obtained; and leaching is carried out. According to the process adopted by the invention, low-temperature leaching is adopted, and methanol leaching with different concentration gradient is adopted, such that taxol is greatly protected and is prevented from conversion during the separation process. Taxol is a biological macromolecular substance, and is susceptible to degradation or isomerization under the influences of environmental conditions such as temperature, organic solvent, acid, alkali, and the like, such that other taxane substances might be produced. For example, under strongly acidic or weakly alkaline environmental conditions, taxol is degraded into baccatin III or is subjected to epitope isomerization to form 7-epipaclitaxel. Under high temperature, taxol is subjected to a degradation reaction to form corresponding small-molecualr substances. With the process provided by the invention, taxol yield is greatly improved.

The invention relates to an apple ecological cultivation method for controlling diseases and pests and improving apple quality. The method comprises the steps that apple seedlings are planted in March or April in the mode of wide rows and dense planting, the apple seedlings with the height of 1 m to 1.5 m are planted on ridges, the apple seedlings are irrigated thoroughly, and soil of the ridges are kept wet; Chinese yew seedlings are planted between every two adjacent apple seedlings on each apple seedling ridge, and the height difference between each apple seedling and each Chinese yew seedling is obvious from July to September; the height of each apple seedling is controlled to range from 2.5 m to 3 m, the height of each Chinese yew seedling is controlled to range from 0.8 m to 1 m. According the apple ecological cultivation method that each Chinese yew seedling is planted between every two adjacent apple seedlings, Chinese yew has the function of pest killing, the roots of the Chinese yew and cut branches, stems, leaves and fruits of the Chinese yew are smashed and applied to an orchard as fertilizer, and therefore apple diseases and pests are controlled in dual modes; in this way, pesticide is not sprayed to the apple seedlings and the Chinese yew seedlings in the whole growth process, and therefore the quality and the taste of apples are guaranteed.

The invention discloses a making method of ancient tea tree black tea and belongs to the technical field of tea processing. The method sequentially comprises steps as follows: (1) tea leaf selection; (2) tea leaf picking; (3) spreading and airing; (4) tea leaf steaming: spread and aired tea leaves are wrapped with wet cotton cloth and placed in a heating tank made of an alder material to be steamed in such a manner that the bottom of the heating tank is heated by steam; (5) spreading and airing again; (6) rolling: 300-400 g of cooled tea leaves are placed on a Chinese yew board to be rolled with palms in the same direction for 30 min; (7) fermentation; (8) drying: four times of drying are performed; (9) static aroma improvement: the tea leaves are stored statically in a dryer for 10-12 h. The black tea made with the method has higher quality, is free of bitterness and astringent and has strong aroma and complete appearance quality, few nutrient ingredients are damaged, and the tea leaves do not break and form strips easily.

The invention relates to a method for preparing yew leaf extract by using high-pressure micro jet and a method for applying yew leaf extract to fabrics, which belong to the technical field of textile chemistry and are characterized by comprising utilizing yew leaves to serve as raw materials, preprocessing and preliminarily grinding the yew leaves, adding 95% of ethanol into the yew leaves, processing the mixture in a high-pressure high-shear micro jet grinder, then performing digestion and vacuum concentration to obtain the yew leaf extract, performing esterification between the yew leaf extract and ultraviolet grafted acrylic fabrics in the presence of condensing agents, and then obtaining fabrics with a certain health care function.

The present invention relates to a method for cultivating Chinese yew by utilizing pot. Said method includes the following steps: a), soil detection; b), cleaning and land preparation; c), seed seedling selection and cultivation; d), applying fertilizer in soil; e), cultivating seedling and f), transplanting. The Chinese yew grown in the pot can be used for releasing beneficial gas to human body to produce the action of sterilization and purifying air.

The invention provides a method for culturing edible fungi by utilizing yew waste residue, comprising the following steps that: the waste residue of the yew after extraction of taxol is dried, crushed and mixed with other raw materials to prepare edible fungi culture medium; the culture medium is sterilized and filled into culture container separately; edible fungi spawns are inoculated into the culture containers for culture; and mycelium and encarpium of the edible fungi are obtained. The method not only takes the yew waste residue as a raw material to change the waste into the valuable and save the cost, but also has the characteristics of high biological effectiveness, good quality and rich nutrient, etc., and has obvious economic benefit and market benefit.

The invention refers to a plant extract to prevent and cure diabetes, extracted from Taxus spp, especially Taxusyunnanensis Cheng et L.K.Fu, Taxus chinensis var.mairei(Lemee et Levl)Cheng et L.K.Fu, etc. It refers to making method and usage. The content of main component of the plant extract powder, myo-inosite derivant compound, can reach above 60%.

The invention relates to a water planting method of Chinese yew. The water planting method comprises steps of plant processing, root growing, and rooting promotion. A conventional plan processing method is used for the plant processing step. According to the root growing step, the relative humidity, the illumination intensity, and the root growing time need to be controlled; and besides, sterilization and immersing based on medicament are also carried out. According to the rooting promotion step, the day temperature, night temperature, relative humidity in air, illumination intensity, and the components and proportion of a nutrient solution need to be controlled. The method has the following advantage: the root growing parameters and rooting promotion parameters are controlled reasonably and all parameters are selected reasonably, and the nutrient solution is used for replacing the soil to cultivate the Chinese yew, so that the Chinese yew can grow well; the whole growth process is easy to control and operation; compared with the soil cultivation, the Chinese yew based on water planting method can grow fast and well; and diseases can be eliminated easily during the growth process based on the advantages of the water planting and thus the growth process and growth quality can be promoted well.

A method for increasing the content of taxol in enqlish yew features that the solution of coronary bacteruim extract onto the stem and leaves of young enqlish yew for regulating its secondary metabolism system and increasing the content of taxol.

The invention discloses a method for extracting taxane type active ingredients from Chinese yew efficiently. The method comprises the following steps of: firstly extracting with water, further extracting with alcohol, and further purifying by a traditional purification method to get paclitaxel, 10-deacetyl baccatin III, taxol-7-xyloside, cephalomanine and other non-water-soluble taxane type substance monomers. According to the method disclosed by the invention, macroporous resin is applied to the extraction process, the extraction yield of the active ingredients is far higher than that of a traditional extraction method, the using quantity of an organic solvent is further significantly reduced, and the method is suitable for large-scale production of the paclitaxel and comprehensive extraction of other taxane active ingredient substances.