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Extraction method of taxus active extract

An extraction method and extract technology are applied in the directions of organic active ingredients, pharmaceutical formulations, medical preparations containing active ingredients, etc., which can solve problems such as large damage and achieve the effect of improving yield

Active Publication Date: 2013-07-24
安徽和华生物医药科技有限公司
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AI Technical Summary

Problems solved by technology

The existing process of extracting active ingredients is very destructive and must be improved

Method used

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Embodiment Construction

[0012] A method for extracting the active extract of taxus paclitaxel, comprising the following process steps:

[0013] (1) Grind yew branches and leaves (3000g dry leaves) to 20-50 mesh, add 5 times the amount of petroleum ether, place at 0-5°C for 10-12 hours, degrease, and filter to obtain filter residue;

[0014] (2) The filter residue is placed in an extraction kettle, and 90% methanol is added to 8-10 times the volume of yew branches and leaves for extraction. The temperature of the extract is -7-5°C, the extraction time is 4-5 hours, and the filtrate is obtained by filtration. Adjust the pH of the filtrate to neutral; add 5 times the amount of 55% methanol to the filter residue for extraction, the temperature of the extract is -10-5°C, the extraction time is 4-5 hours, and the filtrate is obtained by filtration, and the pH of the filtrate is adjusted to neutral; Add 5 times the amount of 45% methanol to the filter residue for extraction, the temperature of the extract i...

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Abstract

The invention relates to an extraction method of a taxus active extract. According to the invention, taxus branches and leaves are crushed to 20-50 meshes; petroleum ether with a 3-5-time amount is added; the mixture is placed under 0-5 DEG C for 10-12h; filtering is carried out, such that a filter slag is obtained; and leaching is carried out. According to the process adopted by the invention, low-temperature leaching is adopted, and methanol leaching with different concentration gradient is adopted, such that taxol is greatly protected and is prevented from conversion during the separation process. Taxol is a biological macromolecular substance, and is susceptible to degradation or isomerization under the influences of environmental conditions such as temperature, organic solvent, acid, alkali, and the like, such that other taxane substances might be produced. For example, under strongly acidic or weakly alkaline environmental conditions, taxol is degraded into baccatin III or is subjected to epitope isomerization to form 7-epipaclitaxel. Under high temperature, taxol is subjected to a degradation reaction to form corresponding small-molecualr substances. With the process provided by the invention, taxol yield is greatly improved.

Description

technical field [0001] The invention relates to an active extract extracted from plants of the genus Tasus of the family Taxaceae. Background technique [0002] Yew is a precious medicinal plant, a rare and endangered tree species in the world, and a national first-class protected plant, known as "plant gold". Among the secondary metabolites of the branches and leaves of Taxus chinensis, its medicinal value contains a very small amount of paclitaxel, which has been widely used clinically to treat various malignant tumors, and is considered to be one of the best anticancer drugs. The existing extraction process of active ingredients is very destructive and must be improved. Contents of the invention [0003] The object of the present invention is to provide a method for extracting the active extract of yew, the extraction conditions are mild, and the yield of paclitaxel extract can reach 0.2-0.3%. [0004] Technical scheme of the present invention is as follows: [0005]...

Claims

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Application Information

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IPC IPC(8): A61K36/13A61K31/337
Inventor 姜艳姜雪琪
Owner 安徽和华生物医药科技有限公司
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