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Taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens and application thereof

A technology of Pseudomonas fluorescens and southern yew, which is applied in the field of biological fertilizers and microbial fertilizers, can solve the problems of screening and application research of highly efficient phosphorus-solubilizing bacteria strains that have not been reported, and achieve the effect of excellent strain resources and promoting growth and development

Inactive Publication Date: 2010-12-01
长治学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the screening and application of bacterial strains with high efficiency in the rhizosphere of Taxus chinensis

Method used

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  • Taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens and application thereof
  • Taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens and application thereof
  • Taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Laboratory phosphorus solubilization test of CLW17 strain.

[0018] Phosphate Solubilizing Medium A: Glucose 10g, Ca 3 (PO 4 ) 3 5g, MgCl 2 5g, KCl 0.2g, MgSO 4 .7H 2 O 0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, pH 7.0.

[0019] Phospholytic medium B: with hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 3 , the other components and contents are the same.

[0020] Phospholytic medium C: ferric phosphate (FePO 4 4H 2 O) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 3 , the other components and contents are the same.

[0021] Phospholytic medium D: aluminum phosphate (AlPO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 3 , the other components and contents are the same.

[0022] Phosphorus-dissolving medium E: use Jiangsu Jinping phosphate rock powder instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 3 , the other components and contents are th...

Embodiment 2

[0025] Embodiment 2: CLW17 greenhouse pot experiment:

[0026]After activating the strain CLW17, use an inoculation loop to pick a small amount of bacteria and inoculate them into a 100mL Erlenmeyer flask containing 50mL of NA medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of distilled water, pH 7.2-7.4), at 29°C , 200r / min shaking culture for 48h. The fermentation broth (4°C, 6000r / min) was centrifuged for 5min, and the bacterial cells were rinsed with sterile normal saline for 3 times, and the bacterial suspension was adjusted with sterile normal saline (10 8 cfu / mL) to make the inoculant. Inoculate Taxus chinensis (seedling age 60 days), with the same amount of sterile saline as the control, and the inoculation amount is 5 mL / plant of seedlings. 6 replicates per treatment were placed in the greenhouse for unified management, the light was 12h / day, and watering was timely.

[0027] The growth of the southern yew 150 days after inoculation is sho...

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PUM

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Abstract

The invention discloses taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens, which is classified and named as pseudomonas fluorescens CLW17 collected in China Center for Type Culture Collection with the collection number of CCTCC NO. M 2010158. The invention further discloses an application of the taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens in promoting the growth of taxus chinensis var. mairei. A strain of the invention has very strong effect of dissolving insoluble phosphates of tricalcium phosphate, iron phosphate, aluminum phosphate, Jiangsu Jinping phosphate rock powder and hydroxylapatite under the situation of being cultured in a liquid shake flask; and the strain is produced into a microbial agent to be respectively inoculated in a cutting seedling and a seedling of the taxus chinensis var. mairei, and the results show that the microbial agent can significantly promote the growth and the development of the two. Therefore, the invention can provide excellent strain resources for special microbial fertilizers for developing the taxus chinensis var. mairei in the future.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and in particular relates to a high-efficiency phosphorus-solubilizing pseudomonas fluorescens in the rhizosphere of the southern yew and an application thereof. Background technique [0002] Southern yew (Taxus chinensis var.mairei) is a species of the genus Taxus in the family Taxaceae. It is a rare and endangered tree species in the world left over from the glacier in the fourth century. It was approved by the State Council in 1999 to be listed as a national first-class protected precious tree species (Yu Yongfu, 1999). The southern yew is scattered in the area south of the Yellow River, including 17 provinces and municipalities including Guangdong, Guangxi, Sichuan, Chongqing, Shanxi, Hunan and Taiwan. [0003] Since the 1970s, the yew plant has attracted the attention of the world because of its special anti-cancer active ingredient pacli...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/39
Inventor 任嘉红刘瑞祥金山刘辉韩明跃
Owner 长治学院
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