The invention discloses an edible fungi culture medium, comprising, by weight, 5-9 parts of a Chinese herb additive, 6-8 parts of wheat bran, 4-6 parts of soybean meal, 15-25 parts of organic residue, 7-9 parts of agricultural straws, 1-3 parts of a trace element additive, 12-16 parts of calcined mineral powder, 20-30 parts of water, 2-4 parts of a PH regulator, 5-9 parts of peat, and 6-8 parts of coarse grain powder. The edible fungi culture medium has rich and balanced nutrition, is loose and breathable and provides greatly increased survival rate and quality for edible fungi; in addition, the Chinese herb additive allows the cultivated edible fungi to be good in safety, free of toxicity, fresh and sweet in taste, and crispy and smooth in taste with certain health effect; the preparation method is simple, and the materials are easy to obtain; a cultivation method of edible fungi herein is simple to perform, has low production cost, allows manpower and materials to be less wasted, provides improved production benefit and product quality, and has high practical value and a promising application prospect.