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Fermentation liquid for culturing pleurotus eryngii matrix, pleurotus eryngii culture medium and preparation method thereof

A technology of eryngium eryngii and fermentation liquid, which is applied in the field of eryngium eryngii culture medium and preparation thereof, and the field of fermentation liquid for cultivating eryngium eryngii substrate, can solve the problems of long time, uncontrolled product quality and the like, and achieves short fruiting time , Fast bacteria growth, good quality effect

Inactive Publication Date: 2015-04-01
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural stacking fermentation is commonly used now, which takes a long time and the product quality is not controlled

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation and Application of Cultivation Substrate Ⅰ

[0020] (1) Preparation of fermentation broth

[0021] Bacillus subtilis CMCC 63501 and Bacillus circus ATCC 4513 were mixed in a medium of 1:1 ratio of peptone 1% (w / v), NaCl 1% (w / v) and yeast powder 0.5% (w / v). LB liquid medium with pH of 7.0 and culture temperature of 37°C was cultured for 24 hours to obtain a fermentation broth.

[0022] (2) Raw material ratio and mixing

[0023] The ratio of raw materials is: 20 parts by weight of cottonseed hulls, 10 parts by weight of sawdust, 12 parts by weight of corncob, 28 parts by weight of bran, 15 parts by weight of bagasse, 13 parts by weight of bean straw, 10 parts by weight of rice bran, 1 part by weight of calcium carbonate , 1 part by weight of lime.

[0024] The above-mentioned raw materials are pulverized to small particles the size of soybean grains, then mixed and mixed well, water is added to the material, the water content of the culture material is adj...

Embodiment 2

[0033] Preparation and Application of Cultivation Substrate Ⅱ

[0034] (1) Preparation of fermentation broth

[0035] Bacillus subtilis and Bacillus circulans in a 3:2 ratio in LB liquid with peptone 1% (W / V) + NaCl 1% (W / V) + yeast powder 0.5% (W / V), pH was 7.2, and the culture temperature was 37°C for 24 hours to obtain a fermentation broth.

[0036] (2) Raw material ratio and mixing

[0037] The ratio of raw materials is: 40 parts by weight of cottonseed hulls, 20 parts by weight of wood chips, 20 parts by weight of corncob, 15 parts by weight of bran, 10 parts by weight of bagasse, 8 parts by weight of bean straw, 5 parts by weight of rice bran, 2 parts by weight of calcium carbonate , 2 parts by weight of lime.

[0038] The above-mentioned raw materials are pulverized to small particles the size of soybean grains, then mixed and mixed well.

[0039] (3) The construction of the compost

[0040] Add 18 parts by weight of fermentation broth into the above-mentioned cult...

Embodiment 3

[0047] Preparation and Application of Cultivation Substrate Ⅲ

[0048] (1) Preparation of fermentation broth

[0049] Bacillus subtilis and Bacillus circulans in the ratio of 6:5 in the medium of peptone 1% (W / V) + NaCl 1% (W / V) + yeast powder 0.5% (W / V) LB liquid, The pH was 6.8 and the culture temperature was 36°C for 23 hours to obtain a fermentation broth.

[0050] (2) Raw material ratio and mixing

[0051] The raw material ratio is: 10 parts by weight of cottonseed hulls, 30 parts by weight of wood chips, 28 parts by weight of corncob, 10 parts by weight of bran, 5 parts by weight of bagasse, 5 parts by weight of bean straw, 15 parts by weight of rice bran, 5 parts by weight of calcium carbonate , 4 parts by weight of lime.

[0052] The above-mentioned raw materials are pulverized to small granules the size of soybean grains, then mixed and mixed well.

[0053] (3) The construction of the compost

[0054] Add 14 parts by weight of fermentation broth into the above-me...

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Abstract

The invention relates to fermentation liquid for culturing pleurotus eryngii matrix, a pleurotus eryngii culture medium and a preparation method thereof, belonging to the technical field of edible fungi culture. The culture matrix is prepared from the following raw materials: cottonseed hull, sawdust, corncob, bran, bagasse, bean stalk, rice bran, calcium carbonate and quick lime; the raw materials are uniformly blended and stockpiled for fermentation together with the microbial fermentation liquid of bacillus subtilis and bacillus circulans; and the decomposition and transformation of organic matters such as lignin and cellulose can be accelerated by use of inoculated fermentation composting, and the nutrient substances such as carbohydrates and carbon nitride compounds required for the mycelial growth of pleurotus eryngii are directly supplied. The pleurotus eryngii culture medium is fully used, the fruiting time is shortened, the biological conversion rate is increased, and the investment cost is saved.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a fermentation broth for culturing a Pleurotus eryngii substrate, a Pleurotus eryngii culture medium and a preparation method thereof. Background technique [0002] Pleurotu seryngii, also known as Pleurotus seryngii, belongs to the phylum Fungi, Eubasidiomycetes, Agaric, Pleurotus genus, and is a precious fungus suitable for both medicine and food. Pleurotus eryngii is thick and tender in texture, especially the color of the stipe is snow-white, thick and long, the organization is extremely dense, firm, and brittle. Its taste is unique, and it is one of the best-tasting mushrooms. "Mushroom King" and "Dried Scallop Mushroom". Pleurotus eryngii is rich in nutrients, contains a variety of amino acids and trace elements, and has active effects such as antioxidant and anti-virus. It is a precious edible fungus with extremely high nutritional value and health care ...

Claims

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Application Information

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IPC IPC(8): C05G1/00C05F17/00
CPCC05D3/02C05F17/50C05F5/002C05F11/00C05F11/08Y02W30/40
Inventor 向文良蔡婷张庆唐洁林凯宋菲菲徐顾榕
Owner XIHUA UNIV
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