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118 results about "Bacillus circulans" patented technology
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Bacillus circulans is a soil-dwelling human pathogen which has been associated with "septicemia, mixed abscess infections, and wound infections", as well as with meningitis.
A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof
InactiveCN101294141AImprove fertilityPromote growthBacteriaOrganic fertilisersDiseaseBacillus megaterium
The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.
Owner:SHANGHAI SIJI BIOLOGICAL TECH
Special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof
InactiveCN101775359AGood degradation activityEasy to storeFungiBacteriaBacillus licheniformisVaccination
The invention discloses a special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof. The special microorganism composite bacterial agent is liquid or solid, and is formed by fermenting, culturing, combining and compositing one or a plurality of bacillus cereus, bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus, bacillus circulans, bacillus sphaerieus, clostridium sporogenes, clostridium acetobutylicum, clostridium barati, clostridium beijerinckii, clostridium thermocellum, trichoderma viride, trichodermareesei and aspergillus niger. Simultaneously, by taking the special microorganism composite bacterial agent as a vaccination bacterial agent, the invention provides an application method for decomposing the crops straws and accelerating the ferment to generate marsh gas; and the microorganism composite bacterial agent-treated crops straws have fast ferment speed, large marsh gas generation quantity, low treating cost and no secondary pollution, can be fermented to generate the marsh gas under the condition of no faeces of poultry, and have very wide application range.
Owner:刘相梅 +1
Composite microbial inoculum organic multielement compound fertilizer and production method thereof
ActiveCN102690151AImprove physicsGood biological propertiesFertilizer mixturesThiobacillus ferrooxidansBran
The invention discloses a composite microbial inoculum organic multielement compound fertilizer which is prepared from microbial inoculum, organic component and inorganic fertilizer. The microbial inoculum is a composite microbial inoculum which comprises the following three types of microbial inocula: resource type microbial inoculum, which contains Bacillus megaterium, thiobacillus thiooxidans, Bacillus mucilaginosus and Bacillus circulans; environment-friendly microbial inoculum, which contains Arthrobacter and Alcaligenes; and antibiotic microbial inoculum, which contains Streptomycs micuoflavus and Streptomyces jingyangensis. The organic component is one or more of cereal crop straw, subsidiary agricultural product dreg, sugar mill bagasse, bran coat, municipal and rural domestic waste, and livestock and poultry dung. The inorganic fertilizer is one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer and medium / micro-nutrient element fertilizer. The weight proportion of microbial inoculum to organic constituent to inorganic fertilizer is (5-25):(18-28):(47-77).
Owner:赵大伟
Multifunctional microorganism soil repairing agent as well as preparation and application thereof
InactiveCN107988096APrevent degradationWide range of repairsBacteriaContaminated soil reclamationDiseaseBacillus licheniformis
The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.
Owner:厦门天邦润生物科技有限公司
High-concentration chemical sewage composite inocula and use thereof
ActiveCN105621626AThe method of treating sewage is simple and easyImprove survival rateBiological water/sewage treatmentSynechococcusBacillus cereus
The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.
Owner:江苏元捷环境科技有限公司
Advanced treatment technology of high-salt-content degradation-resistant oil extraction sewage
InactiveCN105110480AWaste water treatment from quariesBiological water/sewage treatmentPetroleum oilBrevibacillus brevis
The invention discloses a salt-tolerant Brevibacillus brevis and an application of complex microorganisms thereof in advanced treatment of high salt content degradation-resistant oil extraction sewage. The salt-tolerant Brevibacillus brevis is reserved in China General Microbiological Culture Collection Center, CGMCC for short, and the preservation number is CGMCC No.2148. The bacillus can bear high salinity which reaches 70000ppm, and has a wide applicable temperature range. The Brevibacillus brevis has a high COD degradation rate for petroleum hydrocarbon and salt-containing and oil-containing degradation-resistant sewage, and at 25-45 DEG C, the COD degradation rate for high salt content sewage (whose mineralization is higher than 30000ppm) reaches 60%. Brevibacillus brevis, Bacillus subtilis (registration number KC109003.1), Bacillus circulans Jordan (registration number JX893033.1), Bacillus amyloliquefaciens (registration number JX997921.1), Pseudomonas stutzeri (registration number KC113178.1) and Pseudomonas mendocina Marinobacter (registration number EU43329.1) compose complex microorganisms. A synergism is provided for high efficiency degradation of high salt content and oil containing degradation-resistant oil extraction sewage; the COD of incoming water is lower than 350ppm, and the COD of the discharged water after treatment is lower than 60ppm.
Owner:佘跃惠 +1
Beta mannosidase, its coding gene and application
ActiveCN1904052AImprove heat resistanceImprove dissolution efficiencyHydrolasesAnimal feeding stuffAgricultural scienceFeed additive
The present invention relates to a beta-seminase MANB48, its coding gene and application. It is characterized by that said invention uses genome DNA of bacillus circulans as template, and utilizes reverse PCR clone to obtain beta-seminase gene. The total length of said gene is 2079bp, said gene codes a mature beta-seminase containing 372 amino acids and a signal peptide containing 31 amino acids. The invented beta-seminase has good heat resistance and capability of resisting trypsase, and can be used as a feed additive.
Owner:WUHAN SUNHY BIOLOGICAL
Microbial inoculums and preparation method thereof
InactiveCN101724560AImprove product qualityImprove qualityBacteriaMicroorganism based processesDiseaseMicroorganism
The invention discloses a microbial inoculum and a preparation method thereof. The microbial inoculum is prepared from bacterial liquid containing Bacillus subtilis, Bacillus megaterium, Azotobocter chroococcum and Bacillus circulans Jordan and an adsorbent. The adsorbent comprises the following components: humic acid, lignin and diatomite of which the weight ratio is equal to 5:3:1. The proportion of the adsorbent and the bacterial liquid is that: each 100 kg of adsorbent is added with 15 L of bacterial liquid. The effective viable count of the microbial inoculums is between 200,000,000 and 250,000,000 per gram. The microbial inoculum can not only activate the soil and improve the fertilizer utilization, but also effectively inhibit soil-borne disease from breeding and spreading, thereby having the effect of increasing yield.
Owner:山东盛隆生物工程有限公司
Compound microbial agent for low-temperature fermentation of straws
The invention discloses a compound microbial agent for low-temperature fermentation of straws. The compound microbial agent comprises trichoderma viride, aspergillus niger, bacillus subtilis, lactococcus, pediococcus acidilactici and bacillus circulans. In order to prolonging normal-temperature storage time of the compound microbial agent, the compound microbial agent further comprises auxiliary materials including a bacterium preserving agent, a carrier, an antioxidant, deionized water, a water retaining agent and the like. By application of the compound microbial agent for low-temperature fermentation of straws, the straws can be decomposed and blackened about 12 days under the low-temperature condition of 4-15 DEG C, the straws are basically thoroughly decomposed at a high speed; due to addition of the auxiliary materials including the bacterium preserving agent, the carrier, the antioxidant, the water retaining agent and the like into the compound microbial agent for low-temperature fermentation of the straws, the compound microbial agent can be stored for a long time at the normal temperature.
Owner:北京中农国泰科技有限公司
Composite microbial preparation for purifying water and preparation method thereof
ActiveCN102206585AImprove fullyReduce CODBacteriaMicroorganism based processesBacillus licheniformisChemical oxygen demand
The invention discloses a composite microbial preparation for purifying water and a preparation method thereof. The preparation method is characterized by comprising the following steps of: respectively carrying out single-strain culture on bacillus licheniformis, bacillus subtilis, bacillus circulans, xanthan-degrading bacteria and fusiform bacillus to obtain seed culture solutions; inoculating into a same culture medium; then carrying out seed tank fermentation and fermentation tank fermentation culture to obtain the product preparation, wherein the microbial contents of five strains contained in the product preparation are all more than and equal to 1.0*10<9> cfu / ml. According to the preparation method, the strains sufficiently grow, and therefore, the effectiveness of the strains is not reduced and a culture period is also greatly shortened; after a polluted water body is added, the strains respectively perform own functions and cooperatively play roles, and therefore the content of COD (Chemical Oxygen Demand), total nitrogen, total phosphorus and ammonia nitrogen which are contained in water is effectively reduced.
Owner:SHANGHAI SHUIPINGHENG ENVIRONMENT TECH DEV CO LTD
Immobilized microbial consortium for the treatment of phenolic waste-water from petroleum refineries
InactiveUS6406882B1Wider surface areaEasy diffusion of oxygenWater contaminantsOn/in organic carrierBacteroidesFiber
An immobilized microbial consortium is formulated which comprises of a synergistic mixture of all the bacterial strains of Aeromonas hydrophila, Pseudomonas fluorescens, Pseudomonas aeruginosa, Bacillus circulans, Yersinia enterocolitica, Enterobacter cloaca and Bacillus brevis. The formulated microbial consortium is immobilized on a non-biodegradable and economically cheaper support. The said immobilized microbial consortium is used for the biodegradation of synthetic phenol as well as phenol present in petroleum refinery effluent. The results of biodegradation obtained with the microbial consortium immobilized on coconut fiber are compared with those obtained with microbial consortium immobilized on well known support. The coconut fiber used for immobilization proved to be a better support than a well known support such as calcium alginate.
Owner:COUNCIL OF SCI & IND RES
Aquatic compound microecologics and preparation method thereof
The invention discloses aquatic compound microecologics and a preparation method thereof. The compound microecologics is an additive prepared by proportionally mixing four fungi and other fermentation products, and comprises, by weight, 0.20-0.50 part of bacillus subtilis powder, 0.20-0.60 part of bacillus megatherium powder, 0.20-0.50 part of bacillus circulans powder and 0.05-0.30 part of candida tropicalis powder. The compound microecological feed additive has the advantages of simple manufacturing process, easiness to obtain cultures and low cost and has no toxic or side effects and residues. The compound microecological feed additive applied to aquatics can be used as a feed additive so as to improve feed quality, increase crude protein content in feed, decrease coarse fiber content in the feed and improve water quality of aquaculture, thereby being a novel microecologics product which is safe, reliable, pollution-free, purely-fermented and multifunctional.
Owner:TIANJIN CHANGNONG TECH +1
Method for improving cyclization activity of cyclodextrin glucosyltransferase
InactiveCN103966180AIncreased cyclization activityEase of industrial productionBacteriaFermentationGlycineArginine
The invention discloses a method for improving the cyclization activity of cyclodextrin glucosyltransferase, and belongs to the field of genetic engineering and enzyme engineering. Aspartic acid (Asp) at the 577th bit of beta-CGT derived from Bacillus circulans STB01 is mutated into alanine (Ala), glycine (Gly), glutamic acid (Glu), ARGININE (Arg) or lysine (Lys) respectively by a site-directed mutation method; compared with wild CGT, the obtained mutant has remarkably improved cyclization activity and is more suitable for industrial production of cyclodextrin.
Owner:JIANGNAN UNIV
Bacillus circulans WZ-12 and its application in microorganism resolving treatment of dichloromethane
InactiveCN101200697APromote degradationEasy to handleBacteriaMicroorganism based processesMicroorganismHalohydrocarbon
The present invention provides a new strain of bacillus circulans WZ-12 which can degrade methylene chloride and an application of the bacillus circulans WZ-12 for microorganism to decompose and dispose the methylene chloride and preparing for methylene chloride dehalogenase. The bacillus circulans WZ-12 is conserved in the China Center for Typical Culture Collection; the conserving date is 19th, January, 2007; the conserving serial number CCTCC No. is M207006. The beneficial effect of the present invention mainly lies in providing the new strain of the bacillus circulans WZ-12 which can degrade the methylene chloride, the bacillus can degrade the methylene chloride fast under the aerobic condition, which provides important base for researching into the biological degradation mechanism of halohydrocarbon organic pollutant represented by the methylene chloride, and the bacillus can also be applied to a biological disposal technology of organic waste gas directly. Besides, the strain is wild type with very clear genetic background, which is fit for genetic improvement and is hopeful to improve the capability of disposing the organic waste gas greatly.
Owner:ZHEJIANG UNIV OF TECH +2
Bacillus circulans chitoanase as well as preparation method and application thereof
ActiveCN107586768AEnsure safetyImprove efficiencyMicroorganism based processesEnzymesPichia pastorisNeutral proteinase
The invention discloses bacillus circulans chitoanase as well as a preparation method and application thereof. According to the preference of pichia pastoris codons, a chitoanase coding gene sequencein bacillus circulans is obtained by utilizing a whole gene synthesis method; an optimized nucleic acid sequence is shown as SEQ ID NO. 2. Furthermore, efficient secreting expression is carried out onan optimized chitoanase coding gene by utilizing a pichia pastoris expression system to obtain the bacillus circulans chitoanase; an amino acid sequence of the bacillus circulans chitoanase is shownas SEQ ID NO. 1. The chitoanase disclosed by the invention has relatively high hydrolysis activity on a chitosan substrate and a crude enzyme solution produced by shake-flask fermentation has a hydrolysis capability of degrading 5g of chitosan by utilizing 1mL of the crude enzyme solution (with the protein content of about 0.77mg); when the chitosan with the same amount is degraded, about 150mg ofneutral proteinase obtained from bacillus subtilis is needed; the efficiency is theoretically improved by about 200 times; the bacillus circulans chitoanase has a very good industrial application prospect.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI
High-throughput screening method for bacillus circulans
InactiveCN105274086AIncrease screening volumeSpeed up the breeding processMutant preparationMicroorganism based processesLithium chlorideHigh-Throughput Screening Methods
The invention belongs to the technical field of fermentation engineering and microbial breeding, and discloses a high-throughput screening method for bacillus circulans for generating beta-galactosidase. The high-throughput screening method includes main steps of activating initial strains; carrying out ultraviolet mutation and lithium chloride mutation; carrying out high-throughput screening by the aid of 96 pore plates to obtain bacillus circulans mutant strains with improved enzyme capacity. The high-throughput screening method has the advantages that the mutation probability of the strains can be improved by the aid of the traditional physical and chemical combined mutation modes, the screening workload can be reduced by the aid of high-throughput screening tools, the screening efficiency can be improved, strain breeding procedures can be accelerated, and the optimal strains can be screened.
Owner:QUANTUM HI TECH (CHINA) BIO CO LTD
Bacillus series straw fast rotting agent and using method thereof
InactiveCN102876607ACorrupted Time ReductionShorten the durationBacteriaMicroorganism based processesBiotechnologyBacillus licheniformis
The invention relates to a bacillus series straw fast rotting agent and a using method thereof. The fast rotting agent comprises bacillus licheniformis, bacillus circulans, bacillus subtilis, clostridium pasteurianum, bacillus megatherium, jelly-like bacillus, clostridium acetobutylicum, bacteria macerans, bacillus polymyxa and nutrient solution and is used for treating straws to make biological organic fertilizer. The using method comprises the following steps: stacking the straws into a walkway with the width of 40 cm and the height of 30 to 50 cm; uniformly spraying 1.25 kilograms of the fast rotting agent into per ton of straws; controlling the water content of the straws to be 40 to 60 wt%; and rotting at normal temperature for 1 to 2 weeks, wherein when the straws change into brown or black brown, the wet straws are flexible and elastic when being held by hands and the dry straws are crisp and easy to break, a straw decomposition agent product with the nutrient substance content up to 59 weight percent is obtained and can direct serve as fertilizer. By adoption of the fast rotting agent, the straw rotting time is reduced from 15-20 days at present to 7-10 days.
Owner:WUHAN UNIV OF TECH
Microbial fertilizer
InactiveCN105461368AReduce pollutionQuality leapClimate change adaptationOrganic fertilisersBacillus licheniformisBacillus megaterium
The invention relates to a microbial fertilizer. The microbial fertilizer comprises 84-90% by mass of organic matters and 5-11% by mass of water and also comprises a strain, wherein the strain comprises at least two kinds of aerobic bacteria and at least one anaerobic bacterium; each aerobic bacterium is any one of bacillus subtilis, bacillus megaterium, bacillus stearothermophilus, bacillus sphaericus, bacillus firmus and saccharomycetes; the anaerobic bacterium is any one of bacillus circulans, bacillus licheniformis and lactobacillus acidophilus. The microbial fertilizer has the beneficial effects that the microbial fertilizer has the effects of overcoming the defects of the existing chemical fertilizers, reducing environmental pollution and activating soil; the content of beneficial biological bacteria in the microbial fertilizer is more than 0.3billion / g and biological activating enzymes and various trace elements are supplied, so that the quality of crops has a qualitative leap; through simultaneously containing the aerobic bacteria and the anaerobic bacteria, the microbial fertilizer can work under different conditions, thus increasing the output rates of the crops.
Owner:SHANGHAI JINGSHEN TECH
Fermentation liquid for culturing pleurotus eryngii matrix, pleurotus eryngii culture medium and preparation method thereof
InactiveCN104478515AIncrease productionBacteria fastCalcareous fertilisersBio-organic fraction processingFermentationNutrients substances
The invention relates to fermentation liquid for culturing pleurotus eryngii matrix, a pleurotus eryngii culture medium and a preparation method thereof, belonging to the technical field of edible fungi culture. The culture matrix is prepared from the following raw materials: cottonseed hull, sawdust, corncob, bran, bagasse, bean stalk, rice bran, calcium carbonate and quick lime; the raw materials are uniformly blended and stockpiled for fermentation together with the microbial fermentation liquid of bacillus subtilis and bacillus circulans; and the decomposition and transformation of organic matters such as lignin and cellulose can be accelerated by use of inoculated fermentation composting, and the nutrient substances such as carbohydrates and carbon nitride compounds required for the mycelial growth of pleurotus eryngii are directly supplied. The pleurotus eryngii culture medium is fully used, the fruiting time is shortened, the biological conversion rate is increased, and the investment cost is saved.
Owner:XIHUA UNIV
Bacillus circulans and application for same in preparation for ferulic acid decarboxylase
InactiveCN103451133AHigh deacidase activitySimple production processBacteriaMicroorganism based processesYeastUV-Mutagenesis
The invention belongs to the technical field of bioengineering, and particularly discloses bacillus circulans and an application of the same in preparation for ferulic acid decarboxylase. The bacillus circulans is characterized in that a bacillus circulans HG12 strain is obtained by separating from a high-temperature yeast for brewing Maotai-flavour liquor in the white wine plants of China, and screening by virtue of ultraviolet mutation, as well as has the characteristics of being fast in growth and propagation speeds, capable of producing the ferulic acid decarboxylase under the induction of ferulic acid, stable in performance of producing the ferulic acid decarboxylase, and high in enzymatic activity. According to the bacillus circulans and the application disclosed by the invention, continuous and large-scale production for the ferulic acid decarboxylase can be realized, and industrialized production for the ferulic acid decarboxylase is facilitated; moreover, the enzyme is simple and convenient in production process, short in production cycle, and low in cost.
Owner:QILU UNIV OF TECH
Bacillus circulans and application thereof in preparation of galactooligosaccharide by symbiotic fermentation technology
ActiveCN103911322AIncrease profitReduce lossesBacteriaMicroorganism based processesBiotechnologyChromatographic separation
Owner:BAOLINGBAO BIOLOGY
Micro-ecological composite inoculant special for overcoming watermelon continuous cropping obstacle and application method
The invention belongs to the field of microbe fertilizers, and provides a micro-ecological composite inoculant special for overcoming watermelon continuous cropping obstacle and an application method so as to solve the problems that various method for overcoming watermelon continuous cropping obstacle possess responding defects. The inoculant is prepared by mixing bacillus laterosporus, bacillus amyloliquefaciens, bacillus circulans, propionibacterium shermanil, tree microbacterium sp., bacillus laevolacticus, trichoderma harzianum, trichoderma asperellum, trichoderma fasciculatum Bissett, paecilomyces lilacinus, streptomyces syringini, gordonia sp., tsukamurella sp. and conexibacter sp. according to a certain ratio. The composite inoculant guarantees watermelon quality safety and environment zero pollution, and helps to reduce peasant production cost, guarantee agricultural product quality safety and protect ecological environment. The composite inoculant effectively overcomes watermelon continuous cropping obstacle, and watermelon which is truly nuisance-free, pure in mouthfeel and high in sweetness is produced. The composite inoculant possesses significant meaning on watermelon production, watermelon peasant income increase, consumer health guarantee, environment pollution reduction and maintenance of agricultural sustainable development.
Owner:AGRI BIOTECH RES CENT OF SHANXI PROVINCE
Composite fungus agent for preventing blight of vegetable in greenhouse
ActiveCN1568709AConducive to pollution-free productionEasy to exportBiocideAnimal repellantsDiseaseBacillus cereus
A biological control bacteria formulation for preventing green house vegetable disease comprises Bacillus circulans, Bacillus pumilus, Bacillus cereus, Bacillus Subtilis, Serratia marcescens, Pseudomonas fluorescens. The preparation method is : mixing the above-mentioned bacteria according to 1:0.4-0.5:0.2-0.3:0.2-0.3:0.2-0.3:0.2-0.3, 200rpm shaking 36 hours in PDA culture liquid in 33 DEG C, 7000rpm centrifuging, preparing formulation using mixed bacteria and bacteria preservative fluid according to 1:40(g / ml), the alive bacteria concentration being 1x10<9>-1x10<14>cfu / ml. The bacteria formulation can be used in dunghill, root irrigation while transplanting after dilution. The preventing effect of bacteria formulation to vegetable disease can reach 75%, yield promoting effect is more than 45%.
Owner:NANJING XINGUOYUAN ECOLOGICAL AGRI SCI & TECH
Cyclodextrin glycosyltransferase mutants and application thereof
ActiveCN109456950AIncreased receptor affinityImprove conversion rateBacteriaMicroorganism based processesWild typeMethionine biosynthesis
The invention discloses cyclodextrin glycosyltransferase mutants and application thereof and belongs to the technical field of enzyme engineering. Methionine from site 234 of cyclodextrin glycosyltransferase of Bacillus circulans is subjected to site-specific mutagenesis; affinities of mutants M234I, M234A, M234L and M234V to a maltose receptor are increased by 45.6%, 34.7%, 30.3% and 36.9% as compared to wild types. The cyclodextrin glycosyltransferase mutants are applicable to the preparation of trehalose; trehalose conversion rate is further increased.
Owner:JIANGNAN UNIV
Soil microbial conditioner and preparation method thereof
InactiveCN107879852ARich in nutrientsGuarantee the need for growth and reproductionExcrement fertilisersMicroorganism based treatmentBacillus licheniformisBacillus megaterium
The invention relates to a soil microbial conditioner and a preparation method thereof and belongs to the technical field of soil remediation. The soil microbial conditioner is prepared from, in percentage by mass, 20%-40% of functional microbes and 60%-80% of a carrier. The functional microbes comprise one or more of Bacillus laterosporus, Bacillus megaterium, Bacillus mucilaginosus, Bacillus licheniformis, Streptomyces chingyangensis, Lactobacillus plantarum, Bacillus circulans, Pseudomonas stutzeri and Trichoderma viride. The soil microbial conditioner is prepared through culturing and enriching of functional microbes screened from specific soil and attaching of the functional microbes to the carrier, has very high adaptability and colorization capacity for soil, and can quickly regulate balance of microbial florae in the soil, repair soil structures and fundamentally solve the land problems of soil hardening, pesticide residues and the like.
Owner:DALIAN UNIV OF TECH QINGDAO NEW ENERGY MATERIALS TECH RES INST CO LTD
Composite fungus agent for preventing rot knot nematode disease of vegetable in greenhouse
ActiveCN1568710AConducive to pollution-free productionEasy to exportBiocideAnimal repellantsDiseaseGreenhouse
A biological control bacteria formulation for preventing and curing green house vegetable root knot nematode disease is disclosed, specially being used for prevention and cure root knot nematode of cucumber, tomato, pepper etc, which is composed of Bacillus circulans B130 and Bacillus subtilis J1, The preparation method is: mixing the above-mentioned bacteria according to 2:1, 200rpm shaking 24-36 hours in PDA culture liquid in 33 DEG C, 6000rpm centrifuging, preparing formulation using mixed bacteria and bacteria preservative fluid according to 1:40(g / ml), the alive bacteria concentration being 1x10<9>-1x10<12>cfu / ml. The bacteria formulation can be used in dunghill, root irrigation while transplanting after dilution. The preventing effect of bacteria formulation to vegetable root knot nematode disease can reach 30-40%, yield promoting effect is more than 85-96%.
Owner:南京农大生物源农药创制有限公司
Method for producing 4-guaethol through microbial fermentation
InactiveCN103805639AFull of nutritionWide variety of sourcesMicroorganism based processesFermentationMicroorganismVacuum evaporation
The invention belongs to the technical field of bioengineering and particularly relates to a method for producing 4-guaethol through microbial fermentation. A bacillus circulans HG12 strain is used as a production strain in the method. The method is characterized by comprising the steps of inoculating a liquid seed of the HG12 strain into a liquid fermentation medium to ferment to obtain a fermentation solution, filtering the fermentation solution and collecting filtrate; inoculating a liquid seed of a brettanomyces strain into the filtrate to ferment to obtain a 4-guaethol contained fermentation solution; filtering the fermentation solution, collecting the filtrate, and adding an extracting agent into the filtrate to obtain a 4-guaethol contained extracting solution; carrying out vacuum evaporation on the extracting solution to obtain the 4-guaethol. The method can be used for continuously producing 4-guaethol in a large scale; moreover, 4-guaethol is simple and convenient in production process, short in production period, low in production cost and extremely wide in industrial application prospect.
Owner:QILU UNIV OF TECH
Method for producing 4-vinyl guaiacol by fermenting bacillus circulans
InactiveCN103789357AImprove fermentation effectIncrease concentrationMicroorganism based processesFermentationBiotechnologyFeruloyl esterase
The invention belongs to the technical field of biological engineering, and particularly discloses a method for producing 4-vinyl guaiacol by fermenting bacillus circulans. The method takes the bacillus circulans as a producion strain. The method is characterized by comprising the following steps of choosing 1-2 bacillus circulans HG12 thalli from a test tube slant, inoculating to a liquid seed culture medium, and culturing to prepare an activated liquid seed; inoculating the liquid seed into a liquid fermentation culture medium according to inoculum size, and sequentially adding xylanase and feruloyl esterase, culturing to prepare fermentation liquid which contains the 4-vinyl guaiacol; carrying out filtration, extraction and standing phase splitting on the fermentation liquid to prepare extract liquid which contains the 4-vinyl guaiacol, and carrying out vacuum evaporation on the extract liquid to prepare a 4-vinyl guaiacol product. The method disclosed by the invention can realize the continuity and scale of 4-vinyl guaiacol production; the 4-vinyl guaiacol has the advantages of simplicity and convenience for production process, short production period, low production cost and great industrialized application prospect.
Owner:QILU UNIV OF TECH
Saline-alkali soil improving method adopting chitosan soil deep scarification agent
ActiveCN105499264ASimple structureLower pHOther chemical processesContaminated soil reclamationPorosityAlkali soil
The invention provides a saline-alkali soil improving method adopting a chitosan soil deep scarification agent. The method comprises the steps of soil deep scarification agent application, fertilizer application, mechanical deep scarification and irrigation. The soil deep scarification agent is prepared from, by weight, 15-17 parts of chitosan, 5-8 parts of magnesium silicate, 10-15 parts of decomposed coal, 8-12 parts of montmorillonite, 15-18 parts of bacillus circulans bacterial liquid, 8-10 parts of oyster shell powder, 5-6 parts of ammonium persulfate, 7-13 parts of hydrolytic polyacry lonitrile, 1-5 parts of carboxymethylcellulose and 3-6 parts of salicylic acid. Fertilizer application is conducted in the way of applying fermentation fertilizer 20 kg / mu, wherein the fermentation fertilizer is prepared from 10 parts of plant ash, 5 parts of cow dung, 4 parts of rice husk and 9 parts of mushroom bar powder. After soil improvement is conducted for 3 months by means of the method, the total porosity of soil is 55-59%, and the average granular structure of soil is 3.5-4 mm in size.
Owner:WEIFANG YOURONG IND
Cyclodextrin glucosyltransferase mutant with improved cyclization activity
ActiveCN103966190AIncreased cyclization activityEase of industrial productionFermentationGlycosyltransferasesGlycineArginine
The invention discloses a cyclodextrin glucosyltransferase mutant with improved cyclization activity, and belongs to the fields of gene engineering and enzyme engineering. According to the invention, through the adoption of a site-specific mutagenesis method, the 32nd aspartic acid (Asp) derived from beta-CGT enzyme of Bacillus circulans STB01 is mutated into alanine (Ala), glycine (Gly), glutamic acid (Glu), arginine (Arg), lysine (Lys) or glutamine (Gln) respectively. Compared with the cyclization activity of wild CGT enzyme, the cyclization activity of the mutant is obviously improved, so that the mutant is more suitable for industrial production of cyclodextrin.
Owner:JIANGNAN UNIV
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