118 results about "Bacillus circulans" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention discloses a composite microbial inoculum organic multielement compound fertilizer which is prepared from microbial inoculum, organic component and inorganic fertilizer. The microbial inoculum is a composite microbial inoculum which comprises the following three types of microbial inocula: resource type microbial inoculum, which contains Bacillus megaterium, thiobacillus thiooxidans, Bacillus mucilaginosus and Bacillus circulans; environment-friendly microbial inoculum, which contains Arthrobacter and Alcaligenes; and antibiotic microbial inoculum, which contains Streptomycs micuoflavus and Streptomyces jingyangensis. The organic component is one or more of cereal crop straw, subsidiary agricultural product dreg, sugar mill bagasse, bran coat, municipal and rural domestic waste, and livestock and poultry dung. The inorganic fertilizer is one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer and medium/micro-nutrient element fertilizer. The weight proportion of microbial inoculum to organic constituent to inorganic fertilizer is (5-25):(18-28):(47-77).

The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention discloses a microbial inoculum and a preparation method thereof. The microbial inoculum is prepared from bacterial liquid containing Bacillus subtilis, Bacillus megaterium, Azotobocter chroococcum and Bacillus circulans Jordan and an adsorbent. The adsorbent comprises the following components: humic acid, lignin and diatomite of which the weight ratio is equal to 5:3:1. The proportion of the adsorbent and the bacterial liquid is that: each 100 kg of adsorbent is added with 15 L of bacterial liquid. The effective viable count of the microbial inoculums is between 200,000,000 and 250,000,000 per gram. The microbial inoculum can not only activate the soil and improve the fertilizer utilization, but also effectively inhibit soil-borne disease from breeding and spreading, thereby having the effect of increasing yield.

The invention discloses a compound microbial agent for low-temperature fermentation of straws. The compound microbial agent comprises trichoderma viride, aspergillus niger, bacillus subtilis, lactococcus, pediococcus acidilactici and bacillus circulans. In order to prolonging normal-temperature storage time of the compound microbial agent, the compound microbial agent further comprises auxiliary materials including a bacterium preserving agent, a carrier, an antioxidant, deionized water, a water retaining agent and the like. By application of the compound microbial agent for low-temperature fermentation of straws, the straws can be decomposed and blackened about 12 days under the low-temperature condition of 4-15 DEG C, the straws are basically thoroughly decomposed at a high speed; due to addition of the auxiliary materials including the bacterium preserving agent, the carrier, the antioxidant, the water retaining agent and the like into the compound microbial agent for low-temperature fermentation of the straws, the compound microbial agent can be stored for a long time at the normal temperature.

The invention discloses a composite microbial preparation for purifying water and a preparation method thereof. The preparation method is characterized by comprising the following steps of: respectively carrying out single-strain culture on bacillus licheniformis, bacillus subtilis, bacillus circulans, xanthan-degrading bacteria and fusiform bacillus to obtain seed culture solutions; inoculating into a same culture medium; then carrying out seed tank fermentation and fermentation tank fermentation culture to obtain the product preparation, wherein the microbial contents of five strains contained in the product preparation are all more than and equal to 1.0*10<9> cfu/ml. According to the preparation method, the strains sufficiently grow, and therefore, the effectiveness of the strains is not reduced and a culture period is also greatly shortened; after a polluted water body is added, the strains respectively perform own functions and cooperatively play roles, and therefore the content of COD (Chemical Oxygen Demand), total nitrogen, total phosphorus and ammonia nitrogen which are contained in water is effectively reduced.

An immobilized microbial consortium is formulated which comprises of a synergistic mixture of all the bacterial strains of Aeromonas hydrophila, Pseudomonas fluorescens, Pseudomonas aeruginosa, Bacillus circulans, Yersinia enterocolitica, Enterobacter cloaca and Bacillus brevis. The formulated microbial consortium is immobilized on a non-biodegradable and economically cheaper support. The said immobilized microbial consortium is used for the biodegradation of synthetic phenol as well as phenol present in petroleum refinery effluent. The results of biodegradation obtained with the microbial consortium immobilized on coconut fiber are compared with those obtained with microbial consortium immobilized on well known support. The coconut fiber used for immobilization proved to be a better support than a well known support such as calcium alginate.

The invention discloses aquatic compound microecologics and a preparation method thereof. The compound microecologics is an additive prepared by proportionally mixing four fungi and other fermentation products, and comprises, by weight, 0.20-0.50 part of bacillus subtilis powder, 0.20-0.60 part of bacillus megatherium powder, 0.20-0.50 part of bacillus circulans powder and 0.05-0.30 part of candida tropicalis powder. The compound microecological feed additive has the advantages of simple manufacturing process, easiness to obtain cultures and low cost and has no toxic or side effects and residues. The compound microecological feed additive applied to aquatics can be used as a feed additive so as to improve feed quality, increase crude protein content in feed, decrease coarse fiber content in the feed and improve water quality of aquaculture, thereby being a novel microecologics product which is safe, reliable, pollution-free, purely-fermented and multifunctional.

The present invention provides a new strain of bacillus circulans WZ-12 which can degrade methylene chloride and an application of the bacillus circulans WZ-12 for microorganism to decompose and dispose the methylene chloride and preparing for methylene chloride dehalogenase. The bacillus circulans WZ-12 is conserved in the China Center for Typical Culture Collection; the conserving date is 19th, January, 2007; the conserving serial number CCTCC No. is M207006. The beneficial effect of the present invention mainly lies in providing the new strain of the bacillus circulans WZ-12 which can degrade the methylene chloride, the bacillus can degrade the methylene chloride fast under the aerobic condition, which provides important base for researching into the biological degradation mechanism of halohydrocarbon organic pollutant represented by the methylene chloride, and the bacillus can also be applied to a biological disposal technology of organic waste gas directly. Besides, the strain is wild type with very clear genetic background, which is fit for genetic improvement and is hopeful to improve the capability of disposing the organic waste gas greatly.

The invention discloses bacillus circulans chitoanase as well as a preparation method and application thereof. According to the preference of pichia pastoris codons, a chitoanase coding gene sequencein bacillus circulans is obtained by utilizing a whole gene synthesis method; an optimized nucleic acid sequence is shown as SEQ ID NO. 2. Furthermore, efficient secreting expression is carried out onan optimized chitoanase coding gene by utilizing a pichia pastoris expression system to obtain the bacillus circulans chitoanase; an amino acid sequence of the bacillus circulans chitoanase is shownas SEQ ID NO. 1. The chitoanase disclosed by the invention has relatively high hydrolysis activity on a chitosan substrate and a crude enzyme solution produced by shake-flask fermentation has a hydrolysis capability of degrading 5g of chitosan by utilizing 1mL of the crude enzyme solution (with the protein content of about 0.77mg); when the chitosan with the same amount is degraded, about 150mg ofneutral proteinase obtained from bacillus subtilis is needed; the efficiency is theoretically improved by about 200 times; the bacillus circulans chitoanase has a very good industrial application prospect.

The invention relates to a bacillus series straw fast rotting agent and a using method thereof. The fast rotting agent comprises bacillus licheniformis, bacillus circulans, bacillus subtilis, clostridium pasteurianum, bacillus megatherium, jelly-like bacillus, clostridium acetobutylicum, bacteria macerans, bacillus polymyxa and nutrient solution and is used for treating straws to make biological organic fertilizer. The using method comprises the following steps: stacking the straws into a walkway with the width of 40 cm and the height of 30 to 50 cm; uniformly spraying 1.25 kilograms of the fast rotting agent into per ton of straws; controlling the water content of the straws to be 40 to 60 wt%; and rotting at normal temperature for 1 to 2 weeks, wherein when the straws change into brown or black brown, the wet straws are flexible and elastic when being held by hands and the dry straws are crisp and easy to break, a straw decomposition agent product with the nutrient substance content up to 59 weight percent is obtained and can direct serve as fertilizer. By adoption of the fast rotting agent, the straw rotting time is reduced from 15-20 days at present to 7-10 days.

The invention relates to a microbial fertilizer. The microbial fertilizer comprises 84-90% by mass of organic matters and 5-11% by mass of water and also comprises a strain, wherein the strain comprises at least two kinds of aerobic bacteria and at least one anaerobic bacterium; each aerobic bacterium is any one of bacillus subtilis, bacillus megaterium, bacillus stearothermophilus, bacillus sphaericus, bacillus firmus and saccharomycetes; the anaerobic bacterium is any one of bacillus circulans, bacillus licheniformis and lactobacillus acidophilus. The microbial fertilizer has the beneficial effects that the microbial fertilizer has the effects of overcoming the defects of the existing chemical fertilizers, reducing environmental pollution and activating soil; the content of beneficial biological bacteria in the microbial fertilizer is more than 0.3billion/g and biological activating enzymes and various trace elements are supplied, so that the quality of crops has a qualitative leap; through simultaneously containing the aerobic bacteria and the anaerobic bacteria, the microbial fertilizer can work under different conditions, thus increasing the output rates of the crops.

The invention relates to fermentation liquid for culturing pleurotus eryngii matrix, a pleurotus eryngii culture medium and a preparation method thereof, belonging to the technical field of edible fungi culture. The culture matrix is prepared from the following raw materials: cottonseed hull, sawdust, corncob, bran, bagasse, bean stalk, rice bran, calcium carbonate and quick lime; the raw materials are uniformly blended and stockpiled for fermentation together with the microbial fermentation liquid of bacillus subtilis and bacillus circulans; and the decomposition and transformation of organic matters such as lignin and cellulose can be accelerated by use of inoculated fermentation composting, and the nutrient substances such as carbohydrates and carbon nitride compounds required for the mycelial growth of pleurotus eryngii are directly supplied. The pleurotus eryngii culture medium is fully used, the fruiting time is shortened, the biological conversion rate is increased, and the investment cost is saved.

The invention belongs to the technical field of bioengineering, and particularly discloses bacillus circulans and an application of the same in preparation for ferulic acid decarboxylase. The bacillus circulans is characterized in that a bacillus circulans HG12 strain is obtained by separating from a high-temperature yeast for brewing Maotai-flavour liquor in the white wine plants of China, and screening by virtue of ultraviolet mutation, as well as has the characteristics of being fast in growth and propagation speeds, capable of producing the ferulic acid decarboxylase under the induction of ferulic acid, stable in performance of producing the ferulic acid decarboxylase, and high in enzymatic activity. According to the bacillus circulans and the application disclosed by the invention, continuous and large-scale production for the ferulic acid decarboxylase can be realized, and industrialized production for the ferulic acid decarboxylase is facilitated; moreover, the enzyme is simple and convenient in production process, short in production cycle, and low in cost.

A biological control bacteria formulation for preventing green house vegetable disease comprises Bacillus circulans, Bacillus pumilus, Bacillus cereus, Bacillus Subtilis, Serratia marcescens, Pseudomonas fluorescens. The preparation method is : mixing the above-mentioned bacteria according to 1:0.4-0.5:0.2-0.3:0.2-0.3:0.2-0.3:0.2-0.3, 200rpm shaking 36 hours in PDA culture liquid in 33 DEG C, 7000rpm centrifuging, preparing formulation using mixed bacteria and bacteria preservative fluid according to 1:40(g/ml), the alive bacteria concentration being 1x10<9>-1x10<14>cfu/ml. The bacteria formulation can be used in dunghill, root irrigation while transplanting after dilution. The preventing effect of bacteria formulation to vegetable disease can reach 75%, yield promoting effect is more than 45%.

The invention discloses cyclodextrin glycosyltransferase mutants and application thereof and belongs to the technical field of enzyme engineering. Methionine from site 234 of cyclodextrin glycosyltransferase of Bacillus circulans is subjected to site-specific mutagenesis; affinities of mutants M234I, M234A, M234L and M234V to a maltose receptor are increased by 45.6%, 34.7%, 30.3% and 36.9% as compared to wild types. The cyclodextrin glycosyltransferase mutants are applicable to the preparation of trehalose; trehalose conversion rate is further increased.

The invention relates to a soil microbial conditioner and a preparation method thereof and belongs to the technical field of soil remediation. The soil microbial conditioner is prepared from, in percentage by mass, 20%-40% of functional microbes and 60%-80% of a carrier. The functional microbes comprise one or more of Bacillus laterosporus, Bacillus megaterium, Bacillus mucilaginosus, Bacillus licheniformis, Streptomyces chingyangensis, Lactobacillus plantarum, Bacillus circulans, Pseudomonas stutzeri and Trichoderma viride. The soil microbial conditioner is prepared through culturing and enriching of functional microbes screened from specific soil and attaching of the functional microbes to the carrier, has very high adaptability and colorization capacity for soil, and can quickly regulate balance of microbial florae in the soil, repair soil structures and fundamentally solve the land problems of soil hardening, pesticide residues and the like.

The invention belongs to the technical field of biological engineering, and particularly discloses a method for producing 4-vinyl guaiacol by fermenting bacillus circulans. The method takes the bacillus circulans as a producion strain. The method is characterized by comprising the following steps of choosing 1-2 bacillus circulans HG12 thalli from a test tube slant, inoculating to a liquid seed culture medium, and culturing to prepare an activated liquid seed; inoculating the liquid seed into a liquid fermentation culture medium according to inoculum size, and sequentially adding xylanase and feruloyl esterase, culturing to prepare fermentation liquid which contains the 4-vinyl guaiacol; carrying out filtration, extraction and standing phase splitting on the fermentation liquid to prepare extract liquid which contains the 4-vinyl guaiacol, and carrying out vacuum evaporation on the extract liquid to prepare a 4-vinyl guaiacol product. The method disclosed by the invention can realize the continuity and scale of 4-vinyl guaiacol production; the 4-vinyl guaiacol has the advantages of simplicity and convenience for production process, short production period, low production cost and great industrialized application prospect.

The invention provides a saline-alkali soil improving method adopting a chitosan soil deep scarification agent. The method comprises the steps of soil deep scarification agent application, fertilizer application, mechanical deep scarification and irrigation. The soil deep scarification agent is prepared from, by weight, 15-17 parts of chitosan, 5-8 parts of magnesium silicate, 10-15 parts of decomposed coal, 8-12 parts of montmorillonite, 15-18 parts of bacillus circulans bacterial liquid, 8-10 parts of oyster shell powder, 5-6 parts of ammonium persulfate, 7-13 parts of hydrolytic polyacry lonitrile, 1-5 parts of carboxymethylcellulose and 3-6 parts of salicylic acid. Fertilizer application is conducted in the way of applying fermentation fertilizer 20 kg/mu, wherein the fermentation fertilizer is prepared from 10 parts of plant ash, 5 parts of cow dung, 4 parts of rice husk and 9 parts of mushroom bar powder. After soil improvement is conducted for 3 months by means of the method, the total porosity of soil is 55-59%, and the average granular structure of soil is 3.5-4 mm in size.

The invention discloses a cyclodextrin glucosyltransferase mutant with improved cyclization activity, and belongs to the fields of gene engineering and enzyme engineering. According to the invention, through the adoption of a site-specific mutagenesis method, the 32nd aspartic acid (Asp) derived from beta-CGT enzyme of Bacillus circulans STB01 is mutated into alanine (Ala), glycine (Gly), glutamic acid (Glu), arginine (Arg), lysine (Lys) or glutamine (Gln) respectively. Compared with the cyclization activity of wild CGT enzyme, the cyclization activity of the mutant is obviously improved, so that the mutant is more suitable for industrial production of cyclodextrin.