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50 results about "Dendrobium chrysotoxum" patented technology
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Dendrobium chrysotoxum (golden-bow dendrobium or fried-egg orchid) is a widely cultivated species of orchid. It is native to Southeast Asia, and has been found in the wild in Burma (Myanmar), Bhutan, Yunnan (China), Assam (India), Bangladesh, Andaman Islands, Laos, Nepal, Thailand, and Vietnam.
Tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips
InactiveCN102217551ANo pollutionPollution realizedHorticulture methodsPlant tissue cultureBottleEnrichment culture
The invention provides a tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips, which comprises the following steps of: performing induction culture of buds; performing enrichment culture of tufted buds; performing subculture of the tufted buds; and performing rooting culture to obtain a complete plant, and hardening seedling outside a bottle and transplanting. The method is easy to operate and low in production cost, environment pollution is avoided, and large-scale production can be realized. Dendrobium chrysotoxum lindl seedlings cultured by the method are stable in inheritable characters, keep the characteristics of parents and have the advantages of invariance property, small investment, high yield and short period.
Owner:广东省农业科学院花卉研究所
Dendrobium chysotoxum extract and its cosmetic use as an Anti-aging agent
The invention relates to a cosmetic composition comprising at least one extract from the orchid Dendrobium chrysotoxum as an active agent and at least one cosmetically acceptable excipient.The invention relates to the use in a cosmetic composition of an extract from the orchid Dendrobium chrysotoxum as an active agent for preventing or delaying the appearance of the signs of skin ageing or for slowing or attenuating the effects thereof, or else also for promoting cell or tissue longevity.The invention in particular relates to an orchid extract inhibiting the expression and / or the activity of the mitochondrial protein Smac / DIABLO.
Owner:LVMH RECH
Dendrobium health tea and production method thereof
The invention provides a dendrobium health tea which is unadulterated in taste and reasonable in traditional Chinese medicine compatibility and has a plurality of effects, and a production method of the health tea. The dendrobium health tea is prepared from 39%-41% of dendrobium chrysotoxum stem slices, 39%-41% of dendrobium candidum stem segments and 19%-21% of dendrobium chrysotoxum flowers in percentage by weight. The production method of the dendrobium health tea comprises the following steps: firstly, picking the dendrobium chrysotoxum stems, the dendrobium chrysotoxum flowers and the dendrobium candidum stems, cutting the dendrobium chrysotoxum stems into dendrobium chrysotoxum stem slices which are 1mm thick, cutting the dendrobium candidum stems into the dendrobium candidum stem segments which are 0.8cm long, then, performing low-temperature air-drying on the dendrobium chrysotoxum flowers, the dendrobium chrysotoxum stem slices and the dendrobium candidum stem segments respectively, and then mixing and packaging the dendrobium chrysotoxum stem slices, the dendrobium candidum stem segments and the dendrobium chrysotoxum flowers in the weight percentages of 39%-41%, 39%-41% and 19%-21%, thereby obtaining the desired dendrobium health tea. The dendrobium health tea is unadulterated in soup color and taste and reasonable in compatibility, and has a plurality of health care effects.
Owner:QUANZHOU BINGSHANG SHIHU TECH CO LTD
Process for extracting erianin from Dendrobium chrysotoxum Lindl
InactiveCN101973857AEasy to separateSimple extraction methodEther separation/purificationNatural sourceSilica gel
The invention relates to a process for extracting erianin from Dendrobium chrysotoxum Lindl. The process is characterized by comprising the following steps of: preparing a Dendrobium chrysotoxum Lindl ethyl acetate extractive by methods of dipping extraction and microwave-assisted extraction; mixing with aluminum oxide and carrying out column chromatography; eluting with an organic solvent and carrying out chromatography and detecting by a silica gel thin layer; collecting the same fraction as Rf=0.2-0.6 and recovering the reagent; concentrating and crystallizing; recrystallizing with methanol or acetone; and drying to obtain erianin. The extracted compound opens a new approach for developing and applying a new generation of natural source anti-tumor medicaments.
Owner:NANJING ZELANG MEDICAL TECH
PCR detection method of Dendrobium chrysotoxum by adoption of specific primers
ActiveCN102732513ASimple reaction conditionsQuick checkMicrobiological testing/measurementDNA/RNA fragmentationMolecular identificationBiology
The invention provides a PCR detection method of Dendrobium chrysotoxum by the adoption of specific primers. Based on ITS sequence of Dendrobium chrysotoxum, the specific primers F and R (as shown in Seq ID No.1 and 2) which can rapidly detect Dendrobium chrysotoxum are designed, and a PCR detection system is established on this basis so as to provide molecular identification basis for identification of the medicinal material Dendrobium chrysotoxum. According to the invention, Dendrobium chrysotoxum can be rapidly and accurately detected from a plurality of medicinal materials. A detection kit constructed according to the method is simple to operate, has good specificity and high sensitivity, can be used to realize detection of Dendrobium chrysotoxum's stem, leaf or other positions of the plant and medicinal materials, and is suitable for extensive popularization and application.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Healthy dendrobium cordial and preparation method thereof
ActiveCN107460086AHigh in polysaccharidesSignificant liver protection effectDigestive systemAlcoholic beverage preparationDendrobium candidumAlcohol drink
The invention discloses a healthy dendrobium cordial. The healthy dendrobium cordial is a brewed alcohol drink with pure grain liquor as basic liquor, and dendrobium raw materials are added. The weight ratio of the basic liquor to the dendrobium raw materials is 10: 1. The dendrobium raw materials include, by weight, 20% of dendrobium candidum and 80% of dendrobium chrysotoxum. The invention further discloses a preparation method of the healthy dendrobium cordial. By reasonably proportioning dendrobium candidum and dendrobium chrysotoxum, the best health efficacy of the two kinds of dendrobium in the formula can be played, the taste and fragrance of the healthy dendrobium cordial can be regulated, the quality of the healthy dendrobium cordial is improved, and processes are simplified.
Owner:深圳市天之宝生物科技股份有限公司
Method of rapidly propagating dendrobium chrysotoxum tissue cultured seedlings
ActiveCN107466862AHigh transplant survival rateImprove germination rateGrowth substratesCulture mediaBudSeedling
The invention provides a method of rapidly propagating dendrobium chrysotoxum tissue cultured seedlings. Mature dendrobium chrysotoxum capsules are used for explant sampling, culture medium formulas special for all culture stages are designed, and the rapid propagation purpose is achieved through seeds-protocorm-buds. The method includes the following steps of explant selecting and disinfecting, seed germinating and culturing, strong seedling culturing, rooting and culturing and test-tube seedling transplanting. Dendrobium chrysotoxum seedlings can be obtained just after 115-135 days. Seed germination and bud differentiation are conducted synchronously, the culture link is simplified, the culturing cost is reduced, the seed germination rate is high, seedling formation time is short, namely the propagation efficiency is high, and then the whole seedling culturing efficiency is improved. In addition, the cultured test-tube seedlings are strong, have developed root systems and are high in adaptive capacity to environment after being hardened, the seedling transplanting survival rate is increased, and the purpose of rapidly propagating the dendrobium chrysotoxum seedlings is achieved.
Owner:CROP RES INST OF FUJIAN ACAD OF AGRI SCI
Dendrobium chrysotoxum seedling commercialized production method
ActiveCN105309301ADissolve evenlyImprove germination ratePlant genotype modificationBiologyDendrobium chrysotoxum
The invention discloses a dendrobium chrysotoxum seedling commercialized production method, and relates to the field of plant biotechnology. According to the invention, dendrobium chrysotoxum pods are adopted as explants; a modified Ma basic culture medium is adopted; peeled and mashed potato and mashed banana are added during various culture stages; and a dendrobium chrysotoxum sterile rapid propagation system is established, wherein the system comprises related technologies of selective breeding, culture medium preparation, bottled seedling culture, heel-in culture, and the like. With the method, problems of dendrobium chrysotoxum superior seedling source and seedling purity can be effectively solved. Compared to a conventional seedling tissue culture rapid propagation method, with the method, a seed germination rate is improved to higher than 98%, a production cost is reduced by 20%, a growth period is shortened by 60 days, a pollution rate is reduced by 5%, and seedling hardening and transplanting survival rates are improved to higher than 95%. With 60-90 days of heel-in culture, the seedlings can be sold or transplanted. Compared to a division propagation seedling planting method, with the dendrobium chrysotoxum seedlings provided by the invention, 5-7 new sprouts are increased in each cluster.
Owner:YUNNAN DEHONG TROPICAL AGRI RES INST
Chinese mesona herb bean vermicelli and processing method thereof
InactiveCN106072475ASuitable for long-term consumptionHas dual performanceFood ingredient functionsPotato starchYin deficiency
The invention discloses a starch agricultural product, and particularly relates to Chinese mesona herb bean vermicelli. The Chinese mesona herb bean vermicelli is prepared from, by weight, 97-99 parts of potato starch, 260-290 parts of water and 2-2.5 parts of Chinese mesona herb micropowder. The invention further discloses a processing method of the Chinese mesona herb bean vermicelli. The method comprises the steps of raw material selection, preheating and cooling, curing and drying and shaping and packing; the preheating and cooling step comprises the substeps of preheating pulping, stirring, constant-temperature material storing and scraper discharging and shaping, the curing and drying step comprises the substeps of curing, cooling, disengaging, normal-temperature aging and low-temperature aging; the shaping and packing step comprises the substeps of vertical shredding, low-temperature drying, hot-air shaping and drying, cooling, fixed-length crosscutting and inspection and packing. The Chinese mesona herb bean vermicelli is doped with a certain amount of dendrobium chrysotoxum micropowder, the bean vermicelli is elaborately made through full-automation equipment, and the bean vermicelli is tasty and refreshing and delicious, has the effects of health care and disease prevention and is suitable for being eaten by ordinary people for a long time and particularly suitable for yin-deficiency people.
Owner:PUER XINGAN FOOD
Production method for dendrobium chrysotoxum scented tea
InactiveCN103689169AImprove product qualitySweet but not rancidTea substituesNatural stateInflorescence
The invention discloses a production method for dendrobium chrysotoxum scented tea. The method comprises the steps: picking dendrobium chrysotoxum flowers in the morning on fine days, shearing the roots of common peduncles of the dendrobium chrysotoxum flowers, removing discolored flower buds and impurities of taken overall inflorescence of the dendrobium chrysotoxum, picking off flowers, airing in the shade until the moisture content achieves 35-40%; then baking for 10-15 minutes at the temperature of 100-130 DEG C, wherein the moisture content of dendrobium chrysotoxum flowers is less than 6%; spreading for cooling for 2-3 hours, screening to remove the impurities, so as to obtain the dendrobium chrysotoxum scented tea; putting the obtained dendrobium chrysotoxum scented tea in a preservative bag, and tightening the bag. According to the dendrobium chrysotoxum scented tea prepared by adopting the method, the flowers are not withered, and are in natural states, brightly yellow in color without dimness, fibers of the dendrobium chrysotoxum scented tea are thoroughly clear after being brewed with boiling water, the tea is sweet in taste without rancidity, the grade and the quality of the dendrobium chrysotoxum scented tea can be greatly improved, and the dendrobium chrysotoxum scented tea provides a technical support for the industry development for dendrobium chrysotoxum scented tea products. The dendrobium chrysotoxum scented tea can also be used for cooking soup besides being brewed for tea drinking.
Owner:普洱市忠勒佰合农业开发有限公司 +1
Preparing method of dendrobium chrysotoxum lindl wine and prepared dendrobium chrysotoxum lindl wine
PendingCN110106054AImprove filtering effectMeets requirementsAlcoholic beverage preparationFruit juiceBiology
The invention relates to the technical field of alcoholic drinks mixed with fruit juice, in particular to a preparing method of a dendrobium chrysotoxum lindl wine and the prepared dendrobium chrysotoxum lindl wine. The preparing method includes the following steps of firstly, selecting dendrobium chrysotoxum lindl stored for 3 years or longer, and mixing the dendrobium chrysotoxum lindl with basewine for sealed soaking for 90 days or longer, wherein the base wine at least completely soaks dendrobium chrysotoxum lindl; secondly, conducting filtering and taking filtrate. The dendrobium chrysotoxum lindl stored for 3 years or longer is selected and can be stored first, and the dendrobium chrysotoxum lindl can be brewed any time when brewing is needed without being limited by seasons and time; the dendrobium chrysotoxum lindl is directly soaked in the wine, the process is saved, meanwhile the dendrobium chrysotoxum lindl cannot be broken when soaked in the wine, the later-period filtering is quite convenient, the process is simpler, and solids in the wine cannot exceed the standard. The prepared dendrobium chrysotoxum lindl wine is typical in style, mellow and smooth in taste, lasting in aftertaste, continuous and lasting in flowery fragrance and quite in good color, luster and transparency and also has a healthcare function.
Owner:深圳市山海生物科技有限公司
Quality detection method of Dendrobium chrysotoxum Lindley
ActiveCN108398515AAccurate quality evaluationSolve the problem of "curse of dimensionality"Component separationChromatographic separationSchaftoside
The invention discloses a quality detection method of Dendrobium chrysotoxum Lindley. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of a to-be-detected Dendrobium herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / or naringenin as areference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium chrysotoxum Lindley; and4) establishing an analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other componentsin the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component.According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium chrysotoxum Lindley is precisely identified and controlled.
Owner:蓝标一成(海南)生物科技有限公司
New Use of Dendrobium Polypeptide
ActiveCN110856744AIncrease additional utilizationEnhance non-specific immune functionCosmetic preparationsHydrolysed protein ingredientsBiotechnologyImmunity
The invention provides application of Dendrobium polypeptide in resisting oxidation and improving immunity. The method of the invention is adopted to fully utilize herb residues remaining in the actual production of Dendrobium to prepare Dendrobium polypeptide with good activity, and helps improve the additional utilization rate of Dendrobium chrysotoxum Lindl.
Owner:BEIJING UNIV OF CHINESE MEDICINE
Method for identifying dendrobium huoshanense by using chloroplast microsatellite primer pair
ActiveCN106119409ARapid identificationAccurate identificationMicrobiological testing/measurementMolecular identificationDendrobium huoshanense
The invention discloses a method for identifying dendrobium huoshanense by using a chloroplast microsatellite primer pair. The primer pair used for identifying dendrobium huoshanense or assisting identification of dendrobium huoshanense is a primer pair consisting of two single-chain DNA molecules as shown in sequence 1 and sequence 2 in a sequence table. Experiments prove that, by adopting the primer, the dendrobium huoshanense can be quickly and accurately identified from dendrobium wilsonii, dendrobium lohohense, dendrobium chrysotoxum, dendrobium thyrsiflorum, dendrobium lindleyi, dendrobium denndanum, dendrobium loddigesii, dendrobium dixanthum and other adulterants by adopting a quick PCR method, so that technical support is provided to field application of medicinal material molecular identification.
Owner:WEST ANHUI UNIV
New application of dendrobium to preparation of product for preventing and treating angiosteosis
PendingCN111388603AGood effectImprove securityFood ingredient functionsCardiovascular disorderBiotechnologyDendrobium chrysanthum
The invention relates to the technical field of medicines and special medical foods, in particular to a new application of dendrobium to the preparation of a product for preventing and treating angiosteosis. The dendrobium is applied according to a dosage ratio in the preparation of the product for preventing and treating the angiosteosis; an experiment proves that the angiosteosis area can be remarkably reduced and the occurrence and development of the angiosteosis are inhibited; the angiosteosis inhibiting effect of the dendrobium is remarkable; the effect is remarkable and the safety is extremely high, so that the dendrobium has the wide application prospect in angiosteosis prevention and treatment; large-scale artificial planting has been realized; the dendrobium can be one or more ofdendrobium huoshanense, dendrobium officinale, dendrobium chrysanthum, dendrobium fimbriatum, dendrobium chrysotoxum and medicinal dendrobium with the same properties; and the dendrobium is rich in source and relatively low in price, so that the medicine use burden of patients can be reduced.
Owner:安徽同济生生物科技有限公司 +1
Method for treating dendrobium chrysotoxum stem, system thereof and cake capable of protecting intestine and strengthening stomach
InactiveCN110448646ANot easy to mildewDry fastDough treatmentModified nutritive productsIntestinal structureSlice thickness
The invention relates to the technical field of dendrobium chrysotoxum, in particular to a method for treating dendrobium chrysotoxum stem, a system thereof and a cake capable of protecting intestineand strengthening stomach. The treatment method comprises the following steps: S1, picking: selecting dendrobium chrysotoxum with growth of more than 3 years and removing the debris on the surface ofthe stem; S2, flushing: cleaning the dendrobium chrysotoxum stems; S3 slicing: after cleaning, slicing the dendrobium chrysotoxum stem, wherein the slice thickness is less than 5mm; S4, drying; directly drying the dendrobium chrysotoxum stem after slicing in S3 gas, wherein the drying temperature is 110 to 120 DEG C; wherein the sliced dendrobium chrysotoxum in S4 can be subjected to layered placement, the gas enters from the bottom layer, and is discharged from the uppermost layer; the drying time is 20-45min, and the water content of dendrobium chrysotoxum is less than 4%; and S5, cooling; cooling the dendrobium chrysotoxum stems dried in S4 to below 40 DEG C. The dendrobium chrysotoxum stems in S4 are subjected to layered placement, and the gas can quickly take away the moisture on thesurface and inside of the dendrobium chrysotoxum stems, and drying can be rapidly realized.
Owner:深圳市山海生物科技有限公司
Molecular specificity marker primer and method for identifying dendrobium chrysotoxum and dendrobium thyrsiflorum
InactiveCN104313162AStrong subjectivityAccurate methodMicrobiological testing/measurementDNA/RNA fragmentationMolecular identificationAgricultural science
The invention relates to a molecular specificity marker primer and a method for identifying dendrobium chrysotoxum and dendrobium thyrsiflorum. An upstream primer GCF of the molecular specificity marker primer for identifying dendrobium chrysotoxum and dendrobium thyrsiflorum is shown in SEQ ID NO.1 and a downstream primer GCR is shown in SEQ ID NO.2. By using the molecular specificity marker primers GCF / GCR, fast molecular identification on two analogues, namely dendrobium chrysotoxum and dendrobium thyrsiflorum, can be carried out by adopting a common PCR method, and the method is simple. By adopting the PCR technology for detection, the method is short in time and can be accomplished within half a day. The primer and method are effective assistance for identifying dendrobium chrysotoxum and dendrobium thyrsiflorum from morphological characteristics.
Owner:HANGZHOU NORMAL UNIVERSITY
Preparation method of dry dendrobium alcohol extract for spontaneous hypertension
InactiveCN113952417ADecrease mRNA expression levelReduced expression levelMetabolism disorderCardiovascular disorderPharmacyAlcohol
The invention discloses a preparation method of a dry dendrobium alcohol extract for spontaneous hypertension, and relates to the technical field of pharmacy. The problem of tedious preparation is solved. The preparation method specifically comprises the following steps that fresh dendrobium strips are cleaned and dried in a drying box until the water content is less than or equal to 8%; obtained dry dendrobium is crushed and sieved with a 60-100-mesh separation sieve to obtain dry dendrobium powder for later use; 20 g of the dry dendrobium powder is weighed and placed in 350-500 ml of an extracting solution at the temperature of 55-60 DEG C to be repeatedly extracted twice, and extraction lasts for 15-24 h each time is achieved; the two extracts are combined, and filtering and sterilizing are achieved by using a filter membrane to obtain a clear solution; and the clear solution is concentrated under reduced pressure until the volume is 1 / 6-1 / 8 of the original volume, and freeze-drying is achieved until the weight is constant. The fresh dendrobium is one or a combination of more than two of dendrobium huoshanense, dendrobium officinale, dendrobium nobile, dendrobium nobile, dendrobium fimbriatum and dendrobium chrysotoxum. According to the preparation method of the dry dendrobium alcohol extract for the spontaneous hypertension, the preparation method of the dry dendrobium alcohol extract for the spontaneous hypertension can inhibit atherosclerosis and assist in treating hypertension diseases.
Owner:安徽同济生生物科技有限公司
Fluorescent PCR detection kit for discriminating three kinds of medicinal dendrobium in Chinese Pharmacopoeia, and application thereof
ActiveCN108998554AReal-time detectionImprove featuresMicrobiological testing/measurementDNA/RNA fragmentationFluorescenceDna amplification
The invention discloses a fluorescent PCR detection kit for discriminating three kinds of medicinal dendrobium in Pharmacopoeia, and application thereof. The detection kit comprises specific primers and specific probes for Dendrobium nobile, Dendrobium chrysotoxum and Dendrobium fimbriatum, and universal primers and universal probes for Dendrobium, wherein the sequences of the primers and the probes are as shown in SEQ No. 1-12. According to the invention, the specific fluorescent probes are paired with templates, so the detection kit has high specificity; and the detection kit also has high amplification efficiency, high sensitivity, high accuracy, good reproducibility and short detection period, can complete detection within 1.5 hours, is capable of detecting DNA amplification reactionsin real time, and provides powerful guarantee for the quality and authenticity of three medical kinds of medicinal dendrobium recorded in the 2015 edition of Chinese Pharmacopoeia.
Owner:BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
Method for cultivating dendrobium chrysotoxum on stone wall
InactiveCN105191772AIncrease productivityImprove featuresCultivating equipmentsSoilless cultivationStone wallNatural state
The invention relates to a method for cultivating dendrobium chrysotoxum on a stone wall. The method includes the steps that 1, healthy tissue-cultured dendrobium officinale bottle seedlings are selected, and after the seedlings grow out of bottles on a planting field, planting environment adaptation acclimatization is performed for 3-5 months with every 3-5 seedlings being one cluster; 2, bark and wood residues left in wood processing, cow dung and other rejected materials are naturally processed by sufficient sunshine and rainwater, then dried and smashed into powder, the powder is evenly mixed with spent wash to become sticky and thick, and the mixture is made into dendrobium chrysotoxum seedling fertilizer; 3, the dendrobium chrysotoxum seedlings are attached to the stone wall, and the roots are coated with the prepared fertilizer; 4, the dendrobium chrysotoxum seedlings are planted at a reasonable density according to the clustering feature of wild dendrobium chrysotoxum so that adaptive growth in a wild state can be achieved, every bunch is bundled once at the intervals of 80 cm, and every two clusters are bundled each time, wherein the interval between the two cultures is 40 cm; 5, the season with abundant rain, high humidity and proper air temperature is selected for planting, wherein roots and stems grow simultaneously in the season, and the dendrobium chrysotoxum can firmly grow on the stone wall with developed roots after a quarter of growth.
Owner:RUILI LINGRUI AGRI DEV CO LTD
Dendrobium devoninum paxt tea and production method thereof
InactiveCN105639001AEffectively retain nutrientsRetain nutrientsTea substituesDendrobium chrysanthumDendrobium thyrsiflorum
The invention relates to the technical field of health foods and particularly relates to dendrobium devoninum paxt tea and a production method thereof. The dendrobium devoninum paxt tea comprises the following components in percentage by weight: 30% of Dendrobium fimbriatum Hook.var. oculatum Hook, 40% of dendrobium devoninum paxt, 10% of Dendrobium chrysanthum, 5% of Dendrobium thyrsiflorum, 5% of Dendrobium officinale and 10% of Dendrobium chrysotoxum. The dendrobium devoninum paxt is picked from April to June every year. The dendrobium devoninum paxt tea is convenient to eat, moderate in taste and rich in nutrients and can well meet requirements of people.
Owner:RUILI LINGRUI AGRI DEV CO LTD
Plant salt containing sea salt and dendrobium chrysotoxum extract and method for preparing plant salt through chromatography evaporative crystallization process
InactiveCN105831692ASalt low in sodium chlorideReduce the content of heavy metal ionsFilament/thread formingMonocomponent synthetic polymer artificial filamentSorbentNanofiber
The invention provides plant salt containing sea salt and dendrobium chrysotoxum extract .The plant salt containing sea salt and dendrobium chrysotoxum extract is characterized by containing 60-72% of NaCl, the accumulation density ranges from 1.6 g / cm<3> to 1.8 g / cm<3>, and the whiteness ranges from 6% to 79% .The invention further provides a method for preparing the plant salt through a chromatography evaporative crystallization process .The step of removing heavy metal through brine also comprises the step of removing heavy metal through a nanofiber membrane .The step of removing heavy metal through the nanofiber membrane comprises the step of preparing a PAN spinning solution .In the step of preparing the PAN spinning solution, the PAN dissolution temperature ranges from 35 DEG C to 55 DEG C .In the process, a prepared bio-adsorbent enables the brine with the manganese ion content of 22-42 mg / L to be prepared into the plant salt containing sea salt and dendrobium chrysotoxum extract without manganese ions.
Owner:王胜
Dendrobium chysotoxum extract and its cosmetic use as an Anti-aging agent
InactiveUS20150359733A1Prevent and delay appearanceSlow and attenuate effectBiocideCosmetic preparationsActive agentMitochondrial protein
Owner:LVMH RECH
Method for establishing fingerprints of related characteristics of chemical small molecule components of dendrobium chrysotoxum
ActiveCN108205038ASolve the problem of "curse of dimensionality"Content interpretation is highly targetedComponent separationSchaftosideCorrelation analysis
The invention discloses a method for establishing fingerprints of related characteristics of chemical small molecule components of dendrobium chrysotoxum. The method comprises: 1) respectively performing chromatographic detection on samples having a sample size of n, and obtaining test data with chemical small molecule components schaftoside and / or naringenin as reference components; 2) respectively performing fingerprint detection on the samples to obtain a peak area value of the fingerprint of all chemical components of dendrobium chrysotoxum; 3) taking the component content value of schaftoside and / or naringenin in chromatographic data as a response variable, taking a peak area value of other components in the fingerprint as an independent variable to establish an analytical model, andestablishing a linear regression model of the small molecular components and the fingerprints; 4) characterizing the contribution degree of the independent variable to regression modeling through a variable correlation coefficient index, and establishing the fingerprints of the related characteristics of the chemical small molecule components. According to the method, correlation analysis of the chemical small molecule component content is achieved, and the quality of the dendrobium chrysotoxum medicinal material can be effectively identified, controlled and evaluated.
Owner:蓝标一成(海南)生物科技有限公司
PCR detection method of Dendrobium chrysotoxum by adoption of specific primers
ActiveCN102732513BQuick checkAccurate detectionMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyMolecular identification
The invention provides a PCR detection method of Dendrobium chrysotoxum by the adoption of specific primers. Based on ITS sequence of Dendrobium chrysotoxum, the specific primers F and R (as shown in Seq ID No.1 and 2) which can rapidly detect Dendrobium chrysotoxum are designed, and a PCR detection system is established on this basis so as to provide molecular identification basis for identification of the medicinal material Dendrobium chrysotoxum. According to the invention, Dendrobium chrysotoxum can be rapidly and accurately detected from a plurality of medicinal materials. A detection kit constructed according to the method is simple to operate, has good specificity and high sensitivity, can be used to realize detection of Dendrobium chrysotoxum's stem, leaf or other positions of the plant and medicinal materials, and is suitable for extensive popularization and application.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
A method for judging and detecting the similarity of pure Dendrobium chrysanthemum
ActiveCN108874746BJudgment valueThe method is simple and preciseData visualisationBiostatisticsConfidence intervalVersus gene
Owner:SUZHOU QINGLAN BIOMEDICAL TECH CO LTD
Disease control method in dendrobium chrysotoxum planting process
In order to overcome the defects in the prior art, the invention provides a disease control method in the dendrobium chrysotoxum planting process, which comprises the step that disinfectant is sprayed from January to June every year, and the interval between two adjacent times of disinfectant spraying is 20-25 days. The disinfectant is formed by mixing a solid component and clear water with preset mass. The solid component comprises, by mass, 25-28 parts of dandelion stems, 15-20 parts of polygonatum kingianum stems, 50-55 parts of chromolaena odoratum and 10-12 parts of sodium chloride. The disinfectant provided by the invention is prepared by taking conventional herbal medicines and planting byproducts of polygonatum kingianum as main raw materials, and the raw materials are wide in source and relatively low in cost. The disinfectant does not contain chemical components, does not cause excessive pesticide residues of crops, and is convenient for later-stage preparation of a product from the crops. Meanwhile, the obtained disinfectant is good in effect, and the incidence rate of plant diseases and insect pests can be reduced to 3% or below during use.
Owner:普洱淞茂谷林下中药材开发有限公司
A method for identifying Dendrobium Huoshanense with chloroplast microsatellite primer pairs
The invention discloses a method for identifying Dendrobium Huoshanense with chloroplast microsatellite primer pairs. The primer pair for identifying or assisting in identifying Dendrobium Huoshanense provided by the present invention is specifically a primer pair composed of two single-stranded DNA molecules shown in sequence 1 and sequence 2 in the sequence listing. Experiments have proved that the primers provided by the present invention have been used to realize the confusion of Dendrobium Huoshanense and Dendrobium Guangdong, Dendrobium Luohe, Dendrobium chrysanthemum, Dendrobium chinensis, Dendrobium polythene, Dendrobium pleura, Dendrobium meihua and Dendrobium chrysanthemum by the rapid PCR method. Provide technical support for the rapid and accurate identification of medicinal products and the on-site application of molecular identification of medicinal materials.
Owner:WEST ANHUI UNIV
Commercial production method of Dendrobium drumstick seedlings
ActiveCN105309301BDissolve evenlyImprove germination ratePlant genotype modificationContamination ratePhacus
The invention discloses a commercial production method of Dendrobium pachyrhiza seedlings, which belongs to the field of plant biotechnology. The present invention uses the fruit pods of Dendrobium chrysalis as explants, adopts the improved Ma basic medium, adds peeled potato mash and banana mash at each stage of cultivation, and establishes the aseptic and rapid propagation technology system of Dendrobium chrysalis, including improved varieties Related technologies such as breeding, medium preparation, bottle seedling cultivation, and pseudo-planting cultivation can effectively solve the problem of the source of excellent seedlings of Dendrobium drumsticks and the purity of seedlings. Compared with conventional seedling tissue culture and rapid propagation, the seed germination rate Increase to more than 98%, reduce production cost by 20%, shorten the growth cycle by 60 days, reduce the pollution rate by 5%, increase the survival rate of hardening and transplanting to more than 95%, and cultivate fake plants for 60-90 days, which can be sold or transplanted Planting, compared with the planting of ramet propagation seedlings, the present invention cultivates Dendrobium pachyrhiza seedlings planting and increases 5~7 strains of freshly drawn seedlings of each clump.
Owner:YUNNAN DEHONG TROPICAL AGRI RES INST
Multi-center cutting separation method for traditional Chinese medicines
ActiveCN111413419AInnovativeAccurate and effective identificationComponent separationBiotechnologyCentrifugation
The invention relates to the technical field of traditional Chinese medicine effective component separation, and provides a multi-center cutting separation method for traditional Chinese medicines inorder to solve the problems of low analysis sensitivity and low resolution of trace compounds in traditional dendrobium plants, and the method comprises the following steps: (1) crushing raw materialtraditional Chinese medicinal materials, and sieving with a No.3 sieve to obtain traditional Chinese medicine powder; (2) mixing and grinding the traditional Chinese medicine powder and a dispersing agent to obtain a mixture; and (3) filling the mixture into a small solid-phase extraction column, and analyzing the separation effect through elution, centrifugation and sample introduction. Accordingto the method, under a multi-center cutting mode, effective components in dendrobium officinale, dendrobium stem and dendrobium chrysotoxum are separated and identified by utilizing 2D-LC-QTOF-MS andare ingeniously combined, and trace components in traditional Chinese medicines can be conveniently and efficiently separated.
Owner:HANGZHOU NORMAL UNIVERSITY
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