50 results about "Dendrobium chrysotoxum" patented technology

The invention relates to a cosmetic composition comprising at least one extract from the orchid Dendrobium chrysotoxum as an active agent and at least one cosmetically acceptable excipient.

The invention relates to the use in a cosmetic composition of an extract from the orchid Dendrobium chrysotoxum as an active agent for preventing or delaying the appearance of the signs of skin ageing or for slowing or attenuating the effects thereof, or else also for promoting cell or tissue longevity.

The invention in particular relates to an orchid extract inhibiting the expression and/or the activity of the mitochondrial protein Smac/DIABLO.

The invention provides a dendrobium health tea which is unadulterated in taste and reasonable in traditional Chinese medicine compatibility and has a plurality of effects, and a production method of the health tea. The dendrobium health tea is prepared from 39%-41% of dendrobium chrysotoxum stem slices, 39%-41% of dendrobium candidum stem segments and 19%-21% of dendrobium chrysotoxum flowers in percentage by weight. The production method of the dendrobium health tea comprises the following steps: firstly, picking the dendrobium chrysotoxum stems, the dendrobium chrysotoxum flowers and the dendrobium candidum stems, cutting the dendrobium chrysotoxum stems into dendrobium chrysotoxum stem slices which are 1mm thick, cutting the dendrobium candidum stems into the dendrobium candidum stem segments which are 0.8cm long, then, performing low-temperature air-drying on the dendrobium chrysotoxum flowers, the dendrobium chrysotoxum stem slices and the dendrobium candidum stem segments respectively, and then mixing and packaging the dendrobium chrysotoxum stem slices, the dendrobium candidum stem segments and the dendrobium chrysotoxum flowers in the weight percentages of 39%-41%, 39%-41% and 19%-21%, thereby obtaining the desired dendrobium health tea. The dendrobium health tea is unadulterated in soup color and taste and reasonable in compatibility, and has a plurality of health care effects.

The invention relates to a process for extracting erianin from Dendrobium chrysotoxum Lindl. The process is characterized by comprising the following steps of: preparing a Dendrobium chrysotoxum Lindl ethyl acetate extractive by methods of dipping extraction and microwave-assisted extraction; mixing with aluminum oxide and carrying out column chromatography; eluting with an organic solvent and carrying out chromatography and detecting by a silica gel thin layer; collecting the same fraction as Rf=0.2-0.6 and recovering the reagent; concentrating and crystallizing; recrystallizing with methanol or acetone; and drying to obtain erianin. The extracted compound opens a new approach for developing and applying a new generation of natural source anti-tumor medicaments.

The invention provides a PCR detection method of Dendrobium chrysotoxum by the adoption of specific primers. Based on ITS sequence of Dendrobium chrysotoxum, the specific primers F and R (as shown in Seq ID No.1 and 2) which can rapidly detect Dendrobium chrysotoxum are designed, and a PCR detection system is established on this basis so as to provide molecular identification basis for identification of the medicinal material Dendrobium chrysotoxum. According to the invention, Dendrobium chrysotoxum can be rapidly and accurately detected from a plurality of medicinal materials. A detection kit constructed according to the method is simple to operate, has good specificity and high sensitivity, can be used to realize detection of Dendrobium chrysotoxum's stem, leaf or other positions of the plant and medicinal materials, and is suitable for extensive popularization and application.

The invention discloses a healthy dendrobium cordial. The healthy dendrobium cordial is a brewed alcohol drink with pure grain liquor as basic liquor, and dendrobium raw materials are added. The weight ratio of the basic liquor to the dendrobium raw materials is 10: 1. The dendrobium raw materials include, by weight, 20% of dendrobium candidum and 80% of dendrobium chrysotoxum. The invention further discloses a preparation method of the healthy dendrobium cordial. By reasonably proportioning dendrobium candidum and dendrobium chrysotoxum, the best health efficacy of the two kinds of dendrobium in the formula can be played, the taste and fragrance of the healthy dendrobium cordial can be regulated, the quality of the healthy dendrobium cordial is improved, and processes are simplified.

The invention provides a method of rapidly propagating dendrobium chrysotoxum tissue cultured seedlings. Mature dendrobium chrysotoxum capsules are used for explant sampling, culture medium formulas special for all culture stages are designed, and the rapid propagation purpose is achieved through seeds-protocorm-buds. The method includes the following steps of explant selecting and disinfecting, seed germinating and culturing, strong seedling culturing, rooting and culturing and test-tube seedling transplanting. Dendrobium chrysotoxum seedlings can be obtained just after 115-135 days. Seed germination and bud differentiation are conducted synchronously, the culture link is simplified, the culturing cost is reduced, the seed germination rate is high, seedling formation time is short, namely the propagation efficiency is high, and then the whole seedling culturing efficiency is improved. In addition, the cultured test-tube seedlings are strong, have developed root systems and are high in adaptive capacity to environment after being hardened, the seedling transplanting survival rate is increased, and the purpose of rapidly propagating the dendrobium chrysotoxum seedlings is achieved.

The invention discloses a dendrobium chrysotoxum seedling commercialized production method, and relates to the field of plant biotechnology. According to the invention, dendrobium chrysotoxum pods are adopted as explants; a modified Ma basic culture medium is adopted; peeled and mashed potato and mashed banana are added during various culture stages; and a dendrobium chrysotoxum sterile rapid propagation system is established, wherein the system comprises related technologies of selective breeding, culture medium preparation, bottled seedling culture, heel-in culture, and the like. With the method, problems of dendrobium chrysotoxum superior seedling source and seedling purity can be effectively solved. Compared to a conventional seedling tissue culture rapid propagation method, with the method, a seed germination rate is improved to higher than 98%, a production cost is reduced by 20%, a growth period is shortened by 60 days, a pollution rate is reduced by 5%, and seedling hardening and transplanting survival rates are improved to higher than 95%. With 60-90 days of heel-in culture, the seedlings can be sold or transplanted. Compared to a division propagation seedling planting method, with the dendrobium chrysotoxum seedlings provided by the invention, 5-7 new sprouts are increased in each cluster.

The invention discloses a starch agricultural product, and particularly relates to Chinese mesona herb bean vermicelli. The Chinese mesona herb bean vermicelli is prepared from, by weight, 97-99 parts of potato starch, 260-290 parts of water and 2-2.5 parts of Chinese mesona herb micropowder. The invention further discloses a processing method of the Chinese mesona herb bean vermicelli. The method comprises the steps of raw material selection, preheating and cooling, curing and drying and shaping and packing; the preheating and cooling step comprises the substeps of preheating pulping, stirring, constant-temperature material storing and scraper discharging and shaping, the curing and drying step comprises the substeps of curing, cooling, disengaging, normal-temperature aging and low-temperature aging; the shaping and packing step comprises the substeps of vertical shredding, low-temperature drying, hot-air shaping and drying, cooling, fixed-length crosscutting and inspection and packing. The Chinese mesona herb bean vermicelli is doped with a certain amount of dendrobium chrysotoxum micropowder, the bean vermicelli is elaborately made through full-automation equipment, and the bean vermicelli is tasty and refreshing and delicious, has the effects of health care and disease prevention and is suitable for being eaten by ordinary people for a long time and particularly suitable for yin-deficiency people.

The invention discloses a quality detection method of Dendrobium chrysotoxum Lindley. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of a to-be-detected Dendrobium herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and/or naringenin as areference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium chrysotoxum Lindley; and4) establishing an analysis model by using the content value of the schaftoside and/or naringenin in the chromatographic data as the response variable and using the peak area value of other componentsin the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component.According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium chrysotoxum Lindley is precisely identified and controlled.

The invention provides application of Dendrobium polypeptide in resisting oxidation and improving immunity. The method of the invention is adopted to fully utilize herb residues remaining in the actual production of Dendrobium to prepare Dendrobium polypeptide with good activity, and helps improve the additional utilization rate of Dendrobium chrysotoxum Lindl.

The invention discloses a method for identifying dendrobium huoshanense by using a chloroplast microsatellite primer pair. The primer pair used for identifying dendrobium huoshanense or assisting identification of dendrobium huoshanense is a primer pair consisting of two single-chain DNA molecules as shown in sequence 1 and sequence 2 in a sequence table. Experiments prove that, by adopting the primer, the dendrobium huoshanense can be quickly and accurately identified from dendrobium wilsonii, dendrobium lohohense, dendrobium chrysotoxum, dendrobium thyrsiflorum, dendrobium lindleyi, dendrobium denndanum, dendrobium loddigesii, dendrobium dixanthum and other adulterants by adopting a quick PCR method, so that technical support is provided to field application of medicinal material molecular identification.

The invention relates to the technical field of medicines and special medical foods, in particular to a new application of dendrobium to the preparation of a product for preventing and treating angiosteosis. The dendrobium is applied according to a dosage ratio in the preparation of the product for preventing and treating the angiosteosis; an experiment proves that the angiosteosis area can be remarkably reduced and the occurrence and development of the angiosteosis are inhibited; the angiosteosis inhibiting effect of the dendrobium is remarkable; the effect is remarkable and the safety is extremely high, so that the dendrobium has the wide application prospect in angiosteosis prevention and treatment; large-scale artificial planting has been realized; the dendrobium can be one or more ofdendrobium huoshanense, dendrobium officinale, dendrobium chrysanthum, dendrobium fimbriatum, dendrobium chrysotoxum and medicinal dendrobium with the same properties; and the dendrobium is rich in source and relatively low in price, so that the medicine use burden of patients can be reduced.

The invention relates to the technical field of dendrobium chrysotoxum, in particular to a method for treating dendrobium chrysotoxum stem, a system thereof and a cake capable of protecting intestineand strengthening stomach. The treatment method comprises the following steps: S1, picking: selecting dendrobium chrysotoxum with growth of more than 3 years and removing the debris on the surface ofthe stem; S2, flushing: cleaning the dendrobium chrysotoxum stems; S3 slicing: after cleaning, slicing the dendrobium chrysotoxum stem, wherein the slice thickness is less than 5mm; S4, drying; directly drying the dendrobium chrysotoxum stem after slicing in S3 gas, wherein the drying temperature is 110 to 120 DEG C; wherein the sliced dendrobium chrysotoxum in S4 can be subjected to layered placement, the gas enters from the bottom layer, and is discharged from the uppermost layer; the drying time is 20-45min, and the water content of dendrobium chrysotoxum is less than 4%; and S5, cooling; cooling the dendrobium chrysotoxum stems dried in S4 to below 40 DEG C. The dendrobium chrysotoxum stems in S4 are subjected to layered placement, and the gas can quickly take away the moisture on thesurface and inside of the dendrobium chrysotoxum stems, and drying can be rapidly realized.

The invention relates to a molecular specificity marker primer and a method for identifying dendrobium chrysotoxum and dendrobium thyrsiflorum. An upstream primer GCF of the molecular specificity marker primer for identifying dendrobium chrysotoxum and dendrobium thyrsiflorum is shown in SEQ ID NO.1 and a downstream primer GCR is shown in SEQ ID NO.2. By using the molecular specificity marker primers GCF/GCR, fast molecular identification on two analogues, namely dendrobium chrysotoxum and dendrobium thyrsiflorum, can be carried out by adopting a common PCR method, and the method is simple. By adopting the PCR technology for detection, the method is short in time and can be accomplished within half a day. The primer and method are effective assistance for identifying dendrobium chrysotoxum and dendrobium thyrsiflorum from morphological characteristics.

The invention discloses a preparation method of a dry dendrobium alcohol extract for spontaneous hypertension, and relates to the technical field of pharmacy. The problem of tedious preparation is solved. The preparation method specifically comprises the following steps that fresh dendrobium strips are cleaned and dried in a drying box until the water content is less than or equal to 8%; obtained dry dendrobium is crushed and sieved with a 60-100-mesh separation sieve to obtain dry dendrobium powder for later use; 20 g of the dry dendrobium powder is weighed and placed in 350-500 ml of an extracting solution at the temperature of 55-60 DEG C to be repeatedly extracted twice, and extraction lasts for 15-24 h each time is achieved; the two extracts are combined, and filtering and sterilizing are achieved by using a filter membrane to obtain a clear solution; and the clear solution is concentrated under reduced pressure until the volume is 1/6-1/8 of the original volume, and freeze-drying is achieved until the weight is constant. The fresh dendrobium is one or a combination of more than two of dendrobium huoshanense, dendrobium officinale, dendrobium nobile, dendrobium nobile, dendrobium fimbriatum and dendrobium chrysotoxum. According to the preparation method of the dry dendrobium alcohol extract for the spontaneous hypertension, the preparation method of the dry dendrobium alcohol extract for the spontaneous hypertension can inhibit atherosclerosis and assist in treating hypertension diseases.

The invention discloses a fluorescent PCR detection kit for discriminating three kinds of medicinal dendrobium in Pharmacopoeia, and application thereof. The detection kit comprises specific primers and specific probes for Dendrobium nobile, Dendrobium chrysotoxum and Dendrobium fimbriatum, and universal primers and universal probes for Dendrobium, wherein the sequences of the primers and the probes are as shown in SEQ No. 1-12. According to the invention, the specific fluorescent probes are paired with templates, so the detection kit has high specificity; and the detection kit also has high amplification efficiency, high sensitivity, high accuracy, good reproducibility and short detection period, can complete detection within 1.5 hours, is capable of detecting DNA amplification reactionsin real time, and provides powerful guarantee for the quality and authenticity of three medical kinds of medicinal dendrobium recorded in the 2015 edition of Chinese Pharmacopoeia.

The invention relates to a method for cultivating dendrobium chrysotoxum on a stone wall. The method includes the steps that 1, healthy tissue-cultured dendrobium officinale bottle seedlings are selected, and after the seedlings grow out of bottles on a planting field, planting environment adaptation acclimatization is performed for 3-5 months with every 3-5 seedlings being one cluster; 2, bark and wood residues left in wood processing, cow dung and other rejected materials are naturally processed by sufficient sunshine and rainwater, then dried and smashed into powder, the powder is evenly mixed with spent wash to become sticky and thick, and the mixture is made into dendrobium chrysotoxum seedling fertilizer; 3, the dendrobium chrysotoxum seedlings are attached to the stone wall, and the roots are coated with the prepared fertilizer; 4, the dendrobium chrysotoxum seedlings are planted at a reasonable density according to the clustering feature of wild dendrobium chrysotoxum so that adaptive growth in a wild state can be achieved, every bunch is bundled once at the intervals of 80 cm, and every two clusters are bundled each time, wherein the interval between the two cultures is 40 cm; 5, the season with abundant rain, high humidity and proper air temperature is selected for planting, wherein roots and stems grow simultaneously in the season, and the dendrobium chrysotoxum can firmly grow on the stone wall with developed roots after a quarter of growth.

The invention relates to the technical field of health foods and particularly relates to dendrobium devoninum paxt tea and a production method thereof. The dendrobium devoninum paxt tea comprises the following components in percentage by weight: 30% of Dendrobium fimbriatum Hook.var. oculatum Hook, 40% of dendrobium devoninum paxt, 10% of Dendrobium chrysanthum, 5% of Dendrobium thyrsiflorum, 5% of Dendrobium officinale and 10% of Dendrobium chrysotoxum. The dendrobium devoninum paxt is picked from April to June every year. The dendrobium devoninum paxt tea is convenient to eat, moderate in taste and rich in nutrients and can well meet requirements of people.

The invention provides plant salt containing sea salt and dendrobium chrysotoxum extract .The plant salt containing sea salt and dendrobium chrysotoxum extract is characterized by containing 60-72% of NaCl, the accumulation density ranges from 1.6 g/cm<3> to 1.8 g/cm<3>, and the whiteness ranges from 6% to 79% .The invention further provides a method for preparing the plant salt through a chromatography evaporative crystallization process .The step of removing heavy metal through brine also comprises the step of removing heavy metal through a nanofiber membrane .The step of removing heavy metal through the nanofiber membrane comprises the step of preparing a PAN spinning solution .In the step of preparing the PAN spinning solution, the PAN dissolution temperature ranges from 35 DEG C to 55 DEG C .In the process, a prepared bio-adsorbent enables the brine with the manganese ion content of 22-42 mg/L to be prepared into the plant salt containing sea salt and dendrobium chrysotoxum extract without manganese ions.

The invention discloses a method for establishing fingerprints of related characteristics of chemical small molecule components of dendrobium chrysotoxum. The method comprises: 1) respectively performing chromatographic detection on samples having a sample size of n, and obtaining test data with chemical small molecule components schaftoside and/or naringenin as reference components; 2) respectively performing fingerprint detection on the samples to obtain a peak area value of the fingerprint of all chemical components of dendrobium chrysotoxum; 3) taking the component content value of schaftoside and/or naringenin in chromatographic data as a response variable, taking a peak area value of other components in the fingerprint as an independent variable to establish an analytical model, andestablishing a linear regression model of the small molecular components and the fingerprints; 4) characterizing the contribution degree of the independent variable to regression modeling through a variable correlation coefficient index, and establishing the fingerprints of the related characteristics of the chemical small molecule components. According to the method, correlation analysis of the chemical small molecule component content is achieved, and the quality of the dendrobium chrysotoxum medicinal material can be effectively identified, controlled and evaluated.

In order to overcome the defects in the prior art, the invention provides a disease control method in the dendrobium chrysotoxum planting process, which comprises the step that disinfectant is sprayed from January to June every year, and the interval between two adjacent times of disinfectant spraying is 20-25 days. The disinfectant is formed by mixing a solid component and clear water with preset mass. The solid component comprises, by mass, 25-28 parts of dandelion stems, 15-20 parts of polygonatum kingianum stems, 50-55 parts of chromolaena odoratum and 10-12 parts of sodium chloride. The disinfectant provided by the invention is prepared by taking conventional herbal medicines and planting byproducts of polygonatum kingianum as main raw materials, and the raw materials are wide in source and relatively low in cost. The disinfectant does not contain chemical components, does not cause excessive pesticide residues of crops, and is convenient for later-stage preparation of a product from the crops. Meanwhile, the obtained disinfectant is good in effect, and the incidence rate of plant diseases and insect pests can be reduced to 3% or below during use.

The invention relates to the technical field of traditional Chinese medicine effective component separation, and provides a multi-center cutting separation method for traditional Chinese medicines inorder to solve the problems of low analysis sensitivity and low resolution of trace compounds in traditional dendrobium plants, and the method comprises the following steps: (1) crushing raw materialtraditional Chinese medicinal materials, and sieving with a No.3 sieve to obtain traditional Chinese medicine powder; (2) mixing and grinding the traditional Chinese medicine powder and a dispersing agent to obtain a mixture; and (3) filling the mixture into a small solid-phase extraction column, and analyzing the separation effect through elution, centrifugation and sample introduction. Accordingto the method, under a multi-center cutting mode, effective components in dendrobium officinale, dendrobium stem and dendrobium chrysotoxum are separated and identified by utilizing 2D-LC-QTOF-MS andare ingeniously combined, and trace components in traditional Chinese medicines can be conveniently and efficiently separated.