4 results about "Dendrobium chrysotoxum" patented technology

The invention belongs to the technical field of plant protection, and particularly relates to a Tulasnella sp. Fungus strain BQ6-1 and an application of the Tulasnella sp. Fungus strain BQ6-1 in promoting germination of dendrobium cup sheathing seeds, and the preservation number of the fungus strain BQ6-1 is GDMCC No: 66868. The fungus strain BQ6-1 and dendrobium cup sheathing seeds are subjected to symbiotic germination, and the germination rate of the seeds can be remarkably increased. Results of the embodiment show that when the fungus strain BQ6-1 is used for carrying out symbiotic germination on dendrobium cup sheathing seeds, the highest germination rate can reach 88.89%.

The invention discloses a primer probe combination of dendrobium fimbriatum and dendrobium chrysotoxum based on a Proofman-LMTIA technology and application of the primer probe combination, and belongs to the technical field of molecular detection. The primer and probe combination comprises ShiH-F1, ShiH-B1, ShiH-G-LF, ShiH-L-LF, ShiH-G-Probe and ShiH-L-Probe as shown in SEQ ID NO. 1 to 6, and is characterized in that the ShiH-F1, the ShiH-B1, the ShiH-G-LF, the ShiH-L-LF, the ShiH-G-Probe and the ShiH-L-Probe can be used for detecting the content of the According to the invention, a Proofman-LMTIA technology is utilized, specific primers and probes for dendrobium fimbriatum and dendrobium chrysotoxum are designed, a rapid detection method for dendrobium fimbriatum and dendrobium chrysotoxum is established, and technical support is provided for identification of traditional Chinese medicinal materials dendrobium fimbriatum and dendrobium chrysotoxum and identification of adulteration of traditional Chinese medicinal materials.

The application belongs to the field of daily chemicals, and particularly relates to an extraction method and application of Dendrobium chrysostomum, which comprises the following steps: S1. washing and cryopreserving Dendrobium chrysostomum, then vacuum freeze-drying, and crushing after drying to obtain Dendrobium chrysostomum particles; S2. grinding an amphiphilic crystalline polymer, and then mixing the amphiphilic crystalline polymer with the Dendrobium chrysostomum particles to obtain a first mixture; S3. immersing the first mixture in an ethanol aqueous solution for ultrasonic-assisted extraction to obtain a second mixture; S4. performing supercritical carbon dioxide extraction on the second mixture, and then performing reduced-pressure crystallization after extraction to obtain a third mixture; and S5. immersing the third mixture in an ethanol aqueous solution and then performing reduced-pressure crystallization to obtain a Dendrobium chrysostomum extract. The application can realize slow release of effective components and improve skin permeability, and guarantee that active components fully play a role.

The invention belongs to the field of dendrobium cultivation, and provides a live tree epiphytic cultivation method of dendrobium chrysotoxum. The method comprises the following steps: S1, constructing a symbiotic system: binding dendrobium chrysotoxum seedlings and pteridium nursing plants on a selected living host tree from December every year to March next year; s2, fixing dendrobium chrysotoxum seedlings: winding hemp ropes on branches of host trees to fix the dendrobium chrysotoxum seedlings; s3, precise fertilization management: applying an improved nutrient solution, and adjusting the fertilization frequency according to different growth stages of Dendrobium chrysotoxum; s4, monitoring, regulating and controlling polysaccharide accumulation: regularly sampling and determining the polysaccharide content of dendrobium chrysotoxum, and feeding back and adjusting a management strategy according to a determination result. Compared with a traditional living tree planting method, the living tree epiphytic cultivation method of dendrobium chrysotoxum can remarkably improve the survival rate and yield of dendrobium chrysotoxum and the content of polysaccharide medicinal components, and the goal of high-quality and standardized production is achieved.