50 results about "Dendrobium chrysotoxum" patented technology

The invention relates to a cosmetic composition comprising at least one extract from the orchid Dendrobium chrysotoxum as an active agent and at least one cosmetically acceptable excipient.The invention relates to the use in a cosmetic composition of an extract from the orchid Dendrobium chrysotoxum as an active agent for preventing or delaying the appearance of the signs of skin ageing or for slowing or attenuating the effects thereof, or else also for promoting cell or tissue longevity.The invention in particular relates to an orchid extract inhibiting the expression and / or the activity of the mitochondrial protein Smac / DIABLO.

The invention provides a PCR detection method of Dendrobium chrysotoxum by the adoption of specific primers. Based on ITS sequence of Dendrobium chrysotoxum, the specific primers F and R (as shown in Seq ID No.1 and 2) which can rapidly detect Dendrobium chrysotoxum are designed, and a PCR detection system is established on this basis so as to provide molecular identification basis for identification of the medicinal material Dendrobium chrysotoxum. According to the invention, Dendrobium chrysotoxum can be rapidly and accurately detected from a plurality of medicinal materials. A detection kit constructed according to the method is simple to operate, has good specificity and high sensitivity, can be used to realize detection of Dendrobium chrysotoxum's stem, leaf or other positions of the plant and medicinal materials, and is suitable for extensive popularization and application.

The invention relates to a method for judging and detecting the similarity of pure species of Dendrobium chrysalis. The establishment process of the judging method is as follows: S1: collect samples of Dendrobium chrysalis that are consistent with the conclusion of gene sequencing, and measure the total length of each sample; S2: The normality test is carried out on the total length of the sample; S3: standard interval: if the result obtained in step S2 obeys the normal distribution, then the 95% confidence interval of the mean and 95% of the standard deviation are obtained according to the general calculation formula of the normal distribution. % confidence interval; this confidence interval can be used as a standard range to identify the purity of unknown samples. The judgment standard established according to the data actually collected is: the 95% confidence interval of the mean value and the 95% confidence interval of the standard deviation are respectively: (32.6567, 36.2100) and (5.8296, 8.3883), the tested Dendrobium chrysalis sample only needs to match the Compare intervals. The present invention can identify the purity of the species of Dendrobium chrysalis through its morphology-related features, so as to simply judge the value or existence of the artificially planted Dendrobium chrysalis; the identification accuracy is high, and it has important practical significance .

The invention discloses a commercial production method of Dendrobium pachyrhiza seedlings, which belongs to the field of plant biotechnology. The present invention uses the fruit pods of Dendrobium chrysalis as explants, adopts the improved Ma basic medium, adds peeled potato mash and banana mash at each stage of cultivation, and establishes the aseptic and rapid propagation technology system of Dendrobium chrysalis, including improved varieties Related technologies such as breeding, medium preparation, bottle seedling cultivation, and pseudo-planting cultivation can effectively solve the problem of the source of excellent seedlings of Dendrobium drumsticks and the purity of seedlings. Compared with conventional seedling tissue culture and rapid propagation, the seed germination rate Increase to more than 98%, reduce production cost by 20%, shorten the growth cycle by 60 days, reduce the pollution rate by 5%, increase the survival rate of hardening and transplanting to more than 95%, and cultivate fake plants for 60-90 days, which can be sold or transplanted Planting, compared with the planting of ramet propagation seedlings, the present invention cultivates Dendrobium pachyrhiza seedlings planting and increases 5~7 strains of freshly drawn seedlings of each clump.