Dendrobium chrysotoxum seedling commercialized production method

Abstract

The invention discloses a dendrobium chrysotoxum seedling commercialized production method, and relates to the field of plant biotechnology. According to the invention, dendrobium chrysotoxum pods are adopted as explants; a modified Ma basic culture medium is adopted; peeled and mashed potato and mashed banana are added during various culture stages; and a dendrobium chrysotoxum sterile rapid propagation system is established, wherein the system comprises related technologies of selective breeding, culture medium preparation, bottled seedling culture, heel-in culture, and the like. With the method, problems of dendrobium chrysotoxum superior seedling source and seedling purity can be effectively solved. Compared to a conventional seedling tissue culture rapid propagation method, with the method, a seed germination rate is improved to higher than 98%, a production cost is reduced by 20%, a growth period is shortened by 60 days, a pollution rate is reduced by 5%, and seedling hardening and transplanting survival rates are improved to higher than 95%. With 60-90 days of heel-in culture, the seedlings can be sold or transplanted. Compared to a division propagation seedling planting method, with the dendrobium chrysotoxum seedlings provided by the invention, 5-7 new sprouts are increased in each cluster.

Description

technical field [0001] The invention relates to a method for commercially producing seedlings of the edible and medicinal plant Dendrobium pachyrhiza, belonging to the field of plant biotechnology. Background technique [0002] Dendrobium chrysotoxum (Dendrobium chrysotoxum) is a perennial epiphytic herb of the genus Dendrobium orchidaceae, also known as Dendrobium chrysotoxum. The value is extremely high. It can be used as cut flowers and potted plants. The flowers can also be used to develop a series of products such as Dendrobium chrysalis tea, wine, flower cakes, etc. Dendrobium chrysalis is rich in flower resources. It is reported that it contains 22 compounds, which have the functions of improving eyesight, calming the nerves and nourishing the blood. , anti-cancer, anti-depressant, lowering three high effects; its stems can be used medicinally, have the effects of clearing away heat and detoxification, enhancing immunity, etc. Due to the large difference in the flower...

AI Technical Summary

Problems solved by technology

[0003] In recent years, there have been some reports on the tissue culture of Dendrobium chrysalis, and certain tissue culture seedlings have been obtained through tissue culture experiments. However, in the large-scale production technology of Dendrobium, the roots of the cultivated seedlings are thin and few, and the germination rate of the embryos is 75% low. %, the pollution rate is up to 10%, the seedling propagation cycle is long (more than 8 months), and the seedling production cost is up to 1.2 yuan / clump, therefore, carry out the tissue culture breeding research of the sex line of Dendrobium chrysalis, improve the species of Dendrobium chrysalis Seedling commercial production technology also has the following problems:

[0004] 1. Due to the large differences in the traits of the germplasm resources of Dendrobium chrysalis, and relatively stable heredity in terms of yield, the growth of stems, leaves, and flowers is significantly different. Dendrobium chrysalis bears natural fruit. In the absence of light and water and fertilizer, the development of fruit pods is small But not good, the purity of the variety is low, resulting in low germination rate, slow multiplication rate, and poor quality of seedlings

[0005] 2. At present, the seedlings of Dendrobium chrysalis are mainly propagated by division, which leads to a large waste of raw materials and a low germination rate, which is difficult to meet the market demand; while the existing tissue culture technology of Dendrobium chrysalis uses the pods of natural results as explants However, problems such as low germination rate of seed embryos, slow seedling production speed, serious seedling yellowing, and low multiplication multiples lead to slow seedling production speed, poor quality of rooted seedlings, low survival rate of hardening and transplanting, and slow growth. impact on the scale and speed of commercial production of Dendrobium chrysanthemum seedlings