Method of rapidly propagating dendrobium chrysotoxum tissue cultured seedlings

Abstract

The invention provides a method of rapidly propagating dendrobium chrysotoxum tissue cultured seedlings. Mature dendrobium chrysotoxum capsules are used for explant sampling, culture medium formulas special for all culture stages are designed, and the rapid propagation purpose is achieved through seeds-protocorm-buds. The method includes the following steps of explant selecting and disinfecting, seed germinating and culturing, strong seedling culturing, rooting and culturing and test-tube seedling transplanting. Dendrobium chrysotoxum seedlings can be obtained just after 115-135 days. Seed germination and bud differentiation are conducted synchronously, the culture link is simplified, the culturing cost is reduced, the seed germination rate is high, seedling formation time is short, namely the propagation efficiency is high, and then the whole seedling culturing efficiency is improved. In addition, the cultured test-tube seedlings are strong, have developed root systems and are high in adaptive capacity to environment after being hardened, the seedling transplanting survival rate is increased, and the purpose of rapidly propagating the dendrobium chrysotoxum seedlings is achieved.

Description

【Technical field】 [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapidly propagating tissue culture seedlings of Dendrobium drumsticks. 【Background technique】 [0002] Dendrobium chrysotoxum Lindl. (Dendrobium chrysotoxum Lindl.) is a perennial plant of the Orchidaceae Dendrobium genus, also known as Dendrobium jingong, which has both ornamental and medicinal value, and the whole plant is edible; It has the functions of nourishing yin, nourishing the stomach, clearing away heat and detoxifying. It contains anti-tumor active ingredients such as Drumstick Fei and Erianin. It is mostly used for the treatment of chronic pharyngitis, ophthalmic diseases, and thrombotic occlusive diseases, and the effect is very obvious; at the same time, Dendrobium Dendrobium is also an excellent ornamental orchid, with its flower racemes, golden yellow, fragrant and gorgeous Versatile, its stems are spindle-shaped and pecul...

AI Technical Summary

Problems solved by technology

[0004] Although there are some reports on the rapid propagation of Dendrobium drumstick tissue culture in the existing ones, in large-scale production, there are often problems such as low embryo germination rate (less than 75%), long seedling propagation cycle (more than 8 months), transplantation, etc. Problems such as low survival rate (below 85%) are planted, which is extremely unfavorable to the obtaining of high-quality seedlings of Dendrobium drumsticks

In order to solve the problems existing in the tissue culture rapid propagation of these Dendrobium chrysalis seeds, in the Chinese patent whose application number is CN201410674752.6 and titled "a method for tissue culture rapid propagation of Dendrobium chrysalis seeds", it is disclosed that the As material, medium is used for seed germination, differentiation subculture and rooting culture to obtain seedlings; although it has established the tissue culture propagation technology of Dendrobium drumsticks, its germination rate, seedling rate, reproductive efficiency and transplanting survival In addition, in the Chinese patent whose application number is CN201410392173.2 and titled "Commercial Production Method of Dendrobium chrysalis Seedlings", it is proposed to use the pods of Dendrobium chrysalis as material, and adopt improved medium to establish the Although the aseptic and rapid propagation system of Dendrobium candidum has ideal seed germination rate (above 98%), growth cycle (155d) and transplanting survival rate (above 95%), it has the defect that the operation process is relatively complicated.