30results about How to "Seedling time is short" patented technology

The invention provides a method for rapid propagation of pinellia ternate by tissue culture. The method comprises the steps as follows: (1) preparation of culture media; (2) selection of explants; (3)obtaining of callus by induction; (4) multiplication culture of callus; (5) differentiation culture of the callus; (6) acceleration culture; (7) strong seedling culture; (8) acclimatization and transplantation. By use of the special culture media and liquid culture of the callus for 10 d, the volume and weight are increased by 300%, and the quality of the callus is better; when green bud points appear in solid culture media, the callus is transferred to liquid culture media again, the callus differentiates after 10 d of culture, a large number of root systems and bud points appear, and the volume and weight are obviously increased; then, the callus is transferred to the solid culture media, root systems continuously grow, the bud points continuously develop to form a large number of leaves, complete pinellia ternate plants can be formed after 15 d, the propagation coefficient reaches 20 or above, the rooting rate reaches 95% or above, and the transplantation survival rate is 99% or above.

The invention provides a cultivating method of an oncorhynchus mykiss walbaum gynoecial hybrid offspring seed. The method comprises the following steps of: using male sex hormone to induce female oncorhynchus mykiss parent fishes in a sexual maturity stage to obtain oncorhynchus mykiss false milters with male functions; and fertilizing oncorhynchus mykiss walbaum eggs and oncorhynchus mykiss false milter sperms, and incubating to obtain gynoecial hybrid oncorhynchus mykiss walbaum offspring seeds. The eyeing rate of fertilized eggs in an incubating period is more than 83 percent, the offspring seed incubating rate reaches more than 90 percent, through test, the female ratio of the obtained seeds is 100 percent, the oncorhynchus mykiss walbaum ( golden yellow) rate of the offspring seeds reaches more than 91 percent, the weight gain rate of the offspring seed is more than 114 percent after 30-day cultivation, and the survival rate reaches more than 98 percent. Compared with an offspring seed produced by mating of a purebred oncorhynchus mykiss walbaum, according to the cultivating method of the oncorhynchus mykiss walbaum gynoecial hybrid offspring seed provided by the invention, the oncorhynchus mykiss walbaum rate remains unchanged, the oncorhynchus mykiss walbaum gynoecial hybrid offspring seeds with double advantages of hybridization and gynoecious are obtained, and better economic benefit and market value are achieved.

The invention discloses a Qimen rhizoma atractylodis macrocephalae hypocotyl direct regeneration and in-vitro rooting method, and relates to the field of plant biotechnology. The Qimen rhizoma atractylodis macrocephalae hypocotyl direct regeneration and in-vitro rooting method comprises the following steps that (1), explants are pretreated; (2), induction and subculture multiplication culture of adventitious buds are carried out; (3), elongation culture is carried out on adventitious buds; (4), in-vitro rooting treatment is carried out on the elongated buds; and (5), field planting and seedling culture are carried out on the elongated buds. The Qimen rhizoma atractylodis macrocephalae hypocotyl direct regeneration and in-vitro rooting method has the beneficial effects that the breeding process has the advantages of being high in regeneration efficiency, easy to operate, short in seedling forming time, high in seedling forming rate and the like, and a foundation is laid for large-scale breeding and variety improvement of Keemun seedlings in the later period.

The invention relates to the technical field of organic seedling substrate and in particular to a special substrate for float seedling of flue-cured tobaccos and a preparation method of the special substrate. The special substrate for float seedling of flue-cured tobaccos comprises the following components in parts by volume: 6-7 parts of fermented smashed corncobs, 1-2 parts of vermiculite, 1-2 parts of expanded perlite and 0.5-1 part of Magang sand. The substrate disclosed by the invention is high in root activity, high in emergency rate, short in seedling time, good in nutrient absorption and good in overall growth vigor of tobacco seedlings, and all parameters are superior to those of the existing tobacco seedling substrate. By using the special substrate, the corncobs are also sufficiently utilized, so that the problem of environment pollution of the corncobs serving as organic wastes can be solved, the aims of utilizing the wastes and changing waste material into things of valueare achieved, the cyclic utilization of natural resources is realized, and the industrialized production of float seedling of flue-cured tobaccos and the sustainable development of the tobaccos are favorably promoted.

The invention discloses a method for expanding propagation of aphidius gifuensis mummified aphids by utilizing water spinach. The method is characterized in that a tobacco moistening and seedling culture manner is adopted for planting the water spinach as a host plant, aphid inoculation and bee inoculation are respectively carried out in different growth periods of the water spinach for expanding propagation of the aphidius gifuensis mummified aphids, and multi-batch propagation expanding can be carried out during one sowing and planting. The method disclosed by the invention comprises the following concrete steps: a, preparing a propagation expanding chamber; b, sowing the water spinach and managing; c, inoculating aphids and breeding; d, inoculating the aphidius gifuensis, and breeding mummified aphids; e, collecting the aphidius gifuensis mummified aphids; and f, carrying out propagation expanding again. The method disclosed by the invention has the advantages that a flue-cured tobacco moistening and seedling culture manner is used for replacing tobacco as the host plant for breeding the aphidius gifuensis mummified aphids, the characteristics of the water spinach that the water spinach can be easily infected with aphids, a seedling formation period is short and the water spinach can be harvested for multiple times are utilized, short-period multi-batch propagation expanding of the aphidius gifuensis mummified aphids during one planting can be realized, propagation expanding amount is sufficient, propagation cost is low, and an existing tobacco seedling raising facility can be fully utilized, so that rational utilization of resource is realized.

The invention provides a grafting method for pinus massoniana. The method includes the following steps that 1, needle point leaves at grafting positions of stocks are removed, the grafting positions of the stocks are 10 cm-25 cm away from the ground where the stocks are located, and the stocks are nourishing-bag seedlings of annual pinus massoniana; 2, the grafting positions of the stocks are cut one time to expose forming layers; 3, cutting is carried out from the pitch position of the bottom end of each scion to the top end till the 1/3 position away from the top end, and then mild inclined cutting is carried out; 4, the scions obtained through the step3 and the stocks obtained through the step2 are in butt joint. With the nourishing-bag seedlings of annular pinus massoniana being the stocks, the cultivating efficiency of seedlings is improved, the survival rate of the seedlings is increased, and the cost is also greatly lowered.

The invention discloses a tissue culture method for Pruns avium L intraspecific hybridization F1-generation grown-up seedlings. The tissue culture method comprises hybridization seed production and seed collection. A collected seed comprises the following steps of in sequence: S1: carrying out seed hull-breaking processing and seed embryo dipping; S2: carrying out seed embryo disinfection and inner seed coat removal; S3: carrying out inoculation and culture; S4: checking an embryo pollution situation and secondary disinfection; S5: carrying out browning processing and timely transferring; andS6: carrying out acclimatization and transplantation. The tissue culture method only uses one MS solid culture medium which contains an exogenous growth promoting additive, time for taking roots fromgermination is short, the tissue culture method is simple and convenient to operate, hybridization F1-generation seedling growth time is greatly shortened, a whole seed breeding process is shortened,and Pruns avium L breeding efficiency is improved.

The invention relates to a method of lettuce isolated-culture high-frequency regeneration. The method includes the steps that cotyledons and hypocotyls of aseptic lettuce seedlings serve as explants to be subjected to isolated culture and are induced to bud on a budding culture medium, and then, buds are transplanted to a rooting culture medium to form a mass of seedlings. Main components of the budding culture medium include 0.2 mg/L of an MS basic medium, 0.5 mg/L of NAA, 30 g/L of 6-BA and 8 g/L of agar; main components of the rooting culture medium include 0.1 mg/L of an MS basic medium, 30 mg/L of IAA, 30 g/L of sucrose and 8 g/L of agar. According to the method of lettuce isolated-culture high-frequency regeneration, the specific hormone-proportioned media are adopted, the culture period is shortened by 7-10 days compared with current regeneration systems, the differentiation frequency of the explants can achieve 100%, it can be ensured that the seedling regeneration rate reaches95% or above, and experiment and labor costs are saved.

The invention discloses a method for imitating wild cultivation of seedlings via Anoectochilus roxburghii protocorms, which comprises: sterilizing mature capsules of Anoectochilus roxburghii as a material; slitting the capsules; sowing seeds to a culture medium; after the seeds germinate to form protocorms, carrying out propagation culture; directly broadcasting the protocorms into a field humus-rich forest land; building a small arch shed with film; allowing protocorms to grow roots and buds in the small arch shed with certain humidity and to absorb nutrients in nature; removing the film whenseedlings grow 2-3 leaves so that imitating wild propagation of seedlings via Anoectochilus roxburghii protocorms is achieved. The method has the advantages that the growth time of Anoectochilus roxburghii seedlings is short, the survival rate is high, the regularity is good, no variation occurs, no plant growth regulator residue exists during picking, the plant stress resistance is good, the growth is fast, the yield is high, the actual problem in the production of Anoectochilus roxburghii at present is solved, the contradiction between supply and demand of Anoectochilus roxburghii can be effectively relieved, and Anoectochilus roxburghii seedlings are high in yield and quality, the benefit is good, and the cost is low.

The invention belongs to the field of high-quality seedling cultivation and application, and relates to a method for cultivating passion fruit seedlings. According to the method, a filial generation F1 obtained by hybridizing fine-tooth passion flowers and green-fruit passion flowers with high resistance is creatively selected as a stock, tender passion fruit branches with leaf buds are selected as scions, the seedlings cultivated through the grafting method are high in stem base rot resistance, and the branches of the seedlings are luxuriant, so that the seedling renewal planting frequency inthe passion fruit variety planting process is reduced, the cultivation management cost is reduced, the service life of passion fruit plants is prolonged, the harvesting life of the fruits is prolonged, the method has important significance in promoting large-scale planting of the passion fruits, operation is easy, cost is low, and the method is suitable for mass production in agriculture; and themethod has important enlightenment for high-quality seedling cultivation of other passiflora plants.