30results about How to "Seedling time is short" patented technology

The invention relates to a semi-lignified twig cutting and fast root taking and seedling growing method for patchouli. The method comprises the key links of scion-seed stock plant selection, scion picking, root speeding treatment, cutting medium preparation, cutting and cutting post-management. According to the method, the cutting seedlings of the patchouli can root in advance, roots can be taken fast, the cutting survival rate can reach more than 99 percent, sprouting only needs 15 days, and cutting and seedling growing can be carried out all the year round. The method is easy to operate and popularize, a large number of high-quality patchouli seedlings can be cultured in a short time, and a good foundation is laid for the development of the patchouli industry.

The invention provides a method for rapid propagation of pinellia ternate by tissue culture. The method comprises the steps as follows: (1) preparation of culture media; (2) selection of explants; (3)obtaining of callus by induction; (4) multiplication culture of callus; (5) differentiation culture of the callus; (6) acceleration culture; (7) strong seedling culture; (8) acclimatization and transplantation. By use of the special culture media and liquid culture of the callus for 10 d, the volume and weight are increased by 300%, and the quality of the callus is better; when green bud points appear in solid culture media, the callus is transferred to liquid culture media again, the callus differentiates after 10 d of culture, a large number of root systems and bud points appear, and the volume and weight are obviously increased; then, the callus is transferred to the solid culture media, root systems continuously grow, the bud points continuously develop to form a large number of leaves, complete pinellia ternate plants can be formed after 15 d, the propagation coefficient reaches 20 or above, the rooting rate reaches 95% or above, and the transplantation survival rate is 99% or above.

The invention provides a tissue culture method for one-step seedling formation by using with-leaf petioles of Jingzhou pinellia ternate tissue-culture cluster seedlings. The method includes the following steps that 1, explants are selected, wherein Jingzhou pinellia ternate seeds are obtained, after disinfection treatment, germination is accelerated to form cluster buds, and the with-leaf petioles are selected as the explants for tissue culture; 2, the explants are inoculated into a seedling formation medium for culture until the seedlings grow to be 4-10 cm, wherein the seedling formation medium includes 0.5-1.2 mg / L of 1 / 2MS + IBA, 0.2-0.8 mg / L of NAA, 0.15-0.25 mg / L of TDZ, 3% of sucrose and 0.7% of agar. The with-leaf petioles of the Jingzhou pinellia ternate tissue-culture cluster seedlings serve as the explants, the special culture medium is adopted, the seedlings can be rapidly formed in only 45 days, the multiplication coefficient and seedling quality of the Jingzhou pinellia ternate seedlings are ensured (the rooting rate is 95% or more and the transplanting survival rate is 100%), and scale production is achieved.

The invention provides a cultivating method of an oncorhynchus mykiss walbaum gynoecial hybrid offspring seed. The method comprises the following steps of: using male sex hormone to induce female oncorhynchus mykiss parent fishes in a sexual maturity stage to obtain oncorhynchus mykiss false milters with male functions; and fertilizing oncorhynchus mykiss walbaum eggs and oncorhynchus mykiss false milter sperms, and incubating to obtain gynoecial hybrid oncorhynchus mykiss walbaum offspring seeds. The eyeing rate of fertilized eggs in an incubating period is more than 83 percent, the offspring seed incubating rate reaches more than 90 percent, through test, the female ratio of the obtained seeds is 100 percent, the oncorhynchus mykiss walbaum ( golden yellow) rate of the offspring seeds reaches more than 91 percent, the weight gain rate of the offspring seed is more than 114 percent after 30-day cultivation, and the survival rate reaches more than 98 percent. Compared with an offspring seed produced by mating of a purebred oncorhynchus mykiss walbaum, according to the cultivating method of the oncorhynchus mykiss walbaum gynoecial hybrid offspring seed provided by the invention, the oncorhynchus mykiss walbaum rate remains unchanged, the oncorhynchus mykiss walbaum gynoecial hybrid offspring seeds with double advantages of hybridization and gynoecious are obtained, and better economic benefit and market value are achieved.

The invention discloses a radix fici simplicissimae float breeding method. The radix fici simplicissimae float breeding method comprises the following steps: building a seedling raising shed and a seedling raising pond, preparing a matrix, and performing panning and sowing, wherein during seedling raising, the in-shed temperature is controlled to be 20-28 DEG C, and the relative humidity is 60-80%; before seed germination, changing water in the seedling raising pond every 10-15 days; when the sprouting ratio of seeds is more than or equal to 30%, adopting a nutrient to replace water in the seedling raising pond, and changing water every 15-20 days; when each seeding has two leaves, starting to perform thinning and complementary planting of seedlings; when each seedling has three leaves and one core, spraying a plant growth regulator to maintain growth of the seedlings; and performing pest control in the seedling raising period. When the float breeding method is adopted to cultivate radix fici simplicissimae, the cost is lower than that of tissue culture, the seedling formation time is short (the seeding height is 10 cm or more within 30-45 days), the planting percent can reach 95% or more, the obtained seedlings are strong and developed in root system, and the survival rate of transplanting and the planting yield are high.

The invention discloses a Qimen rhizoma atractylodis macrocephalae hypocotyl direct regeneration and in-vitro rooting method, and relates to the field of plant biotechnology. The Qimen rhizoma atractylodis macrocephalae hypocotyl direct regeneration and in-vitro rooting method comprises the following steps that (1), explants are pretreated; (2), induction and subculture multiplication culture of adventitious buds are carried out; (3), elongation culture is carried out on adventitious buds; (4), in-vitro rooting treatment is carried out on the elongated buds; and (5), field planting and seedling culture are carried out on the elongated buds. The Qimen rhizoma atractylodis macrocephalae hypocotyl direct regeneration and in-vitro rooting method has the beneficial effects that the breeding process has the advantages of being high in regeneration efficiency, easy to operate, short in seedling forming time, high in seedling forming rate and the like, and a foundation is laid for large-scale breeding and variety improvement of Keemun seedlings in the later period.

The invention relates to the technical field of organic seedling substrate and in particular to a special substrate for float seedling of flue-cured tobaccos and a preparation method of the special substrate. The special substrate for float seedling of flue-cured tobaccos comprises the following components in parts by volume: 6-7 parts of fermented smashed corncobs, 1-2 parts of vermiculite, 1-2 parts of expanded perlite and 0.5-1 part of Magang sand. The substrate disclosed by the invention is high in root activity, high in emergency rate, short in seedling time, good in nutrient absorption and good in overall growth vigor of tobacco seedlings, and all parameters are superior to those of the existing tobacco seedling substrate. By using the special substrate, the corncobs are also sufficiently utilized, so that the problem of environment pollution of the corncobs serving as organic wastes can be solved, the aims of utilizing the wastes and changing waste material into things of valueare achieved, the cyclic utilization of natural resources is realized, and the industrialized production of float seedling of flue-cured tobaccos and the sustainable development of the tobaccos are favorably promoted.

The invention discloses a method for expanding propagation of aphidius gifuensis mummified aphids by utilizing water spinach. The method is characterized in that a tobacco moistening and seedling culture manner is adopted for planting the water spinach as a host plant, aphid inoculation and bee inoculation are respectively carried out in different growth periods of the water spinach for expanding propagation of the aphidius gifuensis mummified aphids, and multi-batch propagation expanding can be carried out during one sowing and planting. The method disclosed by the invention comprises the following concrete steps: a, preparing a propagation expanding chamber; b, sowing the water spinach and managing; c, inoculating aphids and breeding; d, inoculating the aphidius gifuensis, and breeding mummified aphids; e, collecting the aphidius gifuensis mummified aphids; and f, carrying out propagation expanding again. The method disclosed by the invention has the advantages that a flue-cured tobacco moistening and seedling culture manner is used for replacing tobacco as the host plant for breeding the aphidius gifuensis mummified aphids, the characteristics of the water spinach that the water spinach can be easily infected with aphids, a seedling formation period is short and the water spinach can be harvested for multiple times are utilized, short-period multi-batch propagation expanding of the aphidius gifuensis mummified aphids during one planting can be realized, propagation expanding amount is sufficient, propagation cost is low, and an existing tobacco seedling raising facility can be fully utilized, so that rational utilization of resource is realized.

The invention provides a grafting method for pinus massoniana. The method includes the following steps that 1, needle point leaves at grafting positions of stocks are removed, the grafting positions of the stocks are 10 cm-25 cm away from the ground where the stocks are located, and the stocks are nourishing-bag seedlings of annual pinus massoniana; 2, the grafting positions of the stocks are cut one time to expose forming layers; 3, cutting is carried out from the pitch position of the bottom end of each scion to the top end till the 1 / 3 position away from the top end, and then mild inclined cutting is carried out; 4, the scions obtained through the step3 and the stocks obtained through the step2 are in butt joint. With the nourishing-bag seedlings of annular pinus massoniana being the stocks, the cultivating efficiency of seedlings is improved, the survival rate of the seedlings is increased, and the cost is also greatly lowered.

The invention discloses a tissue culture method for Pruns avium L intraspecific hybridization F1-generation grown-up seedlings. The tissue culture method comprises hybridization seed production and seed collection. A collected seed comprises the following steps of in sequence: S1: carrying out seed hull-breaking processing and seed embryo dipping; S2: carrying out seed embryo disinfection and inner seed coat removal; S3: carrying out inoculation and culture; S4: checking an embryo pollution situation and secondary disinfection; S5: carrying out browning processing and timely transferring; andS6: carrying out acclimatization and transplantation. The tissue culture method only uses one MS solid culture medium which contains an exogenous growth promoting additive, time for taking roots fromgermination is short, the tissue culture method is simple and convenient to operate, hybridization F1-generation seedling growth time is greatly shortened, a whole seed breeding process is shortened,and Pruns avium L breeding efficiency is improved.

The invention discloses a method for expanding propagation of aphidius gifuensis mummified aphids by utilizing water spinach. The method is characterized in that a tobacco moistening and seedling culture manner is adopted for planting the water spinach as a host plant, aphid inoculation and bee inoculation are respectively carried out in different growth periods of the water spinach for expanding propagation of the aphidius gifuensis mummified aphids, and multi-batch propagation expanding can be carried out during one sowing and planting. The method disclosed by the invention comprises the following concrete steps: a, preparing a propagation expanding chamber; b, sowing the water spinach and managing; c, inoculating aphids and breeding; d, inoculating the aphidius gifuensis, and breeding mummified aphids; e, collecting the aphidius gifuensis mummified aphids; and f, carrying out propagation expanding again. The method disclosed by the invention has the advantages that a flue-cured tobacco moistening and seedling culture manner is used for replacing tobacco as the host plant for breeding the aphidius gifuensis mummified aphids, the characteristics of the water spinach that the water spinach can be easily infected with aphids, a seedling formation period is short and the water spinach can be harvested for multiple times are utilized, short-period multi-batch propagation expanding of the aphidius gifuensis mummified aphids during one planting can be realized, propagation expanding amount is sufficient, propagation cost is low, and an existing tobacco seedling raising facility can be fully utilized, so that rational utilization of resource is realized.

The invention discloses a dry rice-nursery method suitable for large-scale production. An open-air cement drying field is selected as a seedbed, straw substrate produced by the company is used, the substrate is laid in a seedling tray, the traying thickness is 1-5 cm, redundant substrate in the seedling tray is removed, and the seedling tray substrate is scraped to be flat and then water is sprayed for wetting; well sown seeds are covered with the substrate and then no germination is required, and the seeds are directly moved to the cement drying field through a forklift for placement in the tray; and when seedling leaf age reaches about 2.5-4 leaves, the seedlings are taken for cutting. The cement drying field is taken as the seedbed without building a nursery greenhouse, no nutrient soilfor seedling culture is used, the straw substrate of the company is used to culture the seedlings, the destruction of soil resources is reduced, the investment is less, management is facilitated, theseedling culture time is short, pests and diseases are fewer, fertilizer is saved by 20%-50%, the seedlings are tidy and robust, the seeds are saved, the root system is developed, the crop maturity period is shortened, and the yield is high.

The invention relates to a method of lettuce isolated-culture high-frequency regeneration. The method includes the steps that cotyledons and hypocotyls of aseptic lettuce seedlings serve as explants to be subjected to isolated culture and are induced to bud on a budding culture medium, and then, buds are transplanted to a rooting culture medium to form a mass of seedlings. Main components of the budding culture medium include 0.2 mg / L of an MS basic medium, 0.5 mg / L of NAA, 30 g / L of 6-BA and 8 g / L of agar; main components of the rooting culture medium include 0.1 mg / L of an MS basic medium, 30 mg / L of IAA, 30 g / L of sucrose and 8 g / L of agar. According to the method of lettuce isolated-culture high-frequency regeneration, the specific hormone-proportioned media are adopted, the culture period is shortened by 7-10 days compared with current regeneration systems, the differentiation frequency of the explants can achieve 100%, it can be ensured that the seedling regeneration rate reaches95% or above, and experiment and labor costs are saved.

The invention provides a cultivation method for hybridized all-female golden trout fries. An androgen is utilized to induce golden trout parent fish in a mature period so as to obtain golden trout pseudo-milter, steelhead parent fish eggs are fertilized with golden trout pseudo-milter sperms, hatching is performed, and the hybridized all-female golden trout fries are obtained. The eyed rate of the fertilized eggs during a hatching period is over 81%, the fry hatching rate is over 92%, the female rate of the obtained fries is 100% by detection, the rate of golden trout (with golden yellow body color) of the fries is over 90%, the weight gaining rate is over 122% after the fries are cultivated for 30 days, and the survival rate is over 98%. Compared with fries generated by purebred golden trout mating, the cultivation method for the hybridized all-female golden trout fries maintains the golden trout rate to be unchanged, enables the golden trout fries having double advantages of being hybridized and all-female to be obtained, and has good economic benefit and market value.

The invention discloses a paddy soilless transplant-free quick seedling raising method and equipment. The method comprises the following steps: a, preparing seedling raising nets, i.e., manufacturing square seedling raising nets by using a perishable and degradable biomass material, wherein the two ends of the seedling raising nets are fixed to fixing rods; b, transplanting seeds, i.e., transplanting the seeds in the seedling raising nets, wherein one ends of the seeds with embryos are exposed outside the net material and are in the same direction, and 2-3 seeds are transplanted for each plant; c, soaking to sprout, i.e., placing and soaking the seedling raising nets in which the seeds are transplanted in the previous step in a sprouting pool to sprout, and draining water in the pool and covering with thin films when the seeds are budded; and d, putting on the rack to raise seedlings, i.e., moving the seedling raising nets to an outdoor seedling raising rack when the seedlings are grown to 1-2 mm, covering with the thin films, watering once per hour, reserving holes in the thin films at the two ends of the seedling raising rack to ventilate when the seedlings are grown to 1.5 cm, removing the thin films to expose the seedlings in air when the seedlings in the seedling raising rack are grown to 8-10 cm, and transplanting into a paddy field after removing the thin film for 3-4 days.

The invention discloses a tissue culture method for rapid growth of sweet cherry intraspecific hybrid F1 generation seedlings, which includes hybrid seed production and seed collection; the collected seeds are sequentially subjected to the following steps: S1, seed breaking treatment and seed embryo soaking; S2 1. Embryo disinfection and inner testa removal; S3, inoculation and cultivation; S4, inspection of embryo contamination and secondary disinfection; S5, browning treatment and timely transfer; S6, seedling hardening and transplanting. The present invention only uses a MS solid medium containing exogenous growth-promoting additives, the time required from germination to rooting is relatively short, the operation is simple and convenient, and the time for seedling formation of the hybrid F1 generation is greatly shortened, the entire breeding process is shortened, and sweetness is improved. Cherry breeding efficiency.

The invention discloses a method for directly regenerating hypocotyls and rooting in vitro, and relates to the field of plant biotechnology. The invention includes the following steps: (1) pretreatment of explants; (2) induction and subculture of adventitious buds Proliferation culture; (3) elongation culture of adventitious buds; (4) in vitro rooting treatment of elongated buds; (5) colonization and seedling cultivation of elongated buds. The beneficial effect of the present invention is that: the breeding process involved in the present invention has the advantages of high regeneration efficiency, simple operation, short seedling time, high seedling rate, etc., which lays the foundation for the large-scale breeding and variety improvement of later Qishu seedlings.

The invention provides a method for rapidly propagating tissue-cultured seedlings of Dendrobium chrysalis, which uses the mature capsule of Dendrobium chrysalis as the object of explants, designs special medium formulas for each cultivation stage, and achieves the purpose of rapid propagation through seeds-protocorms-buds The method comprises the following steps: selection and disinfection of explants, seed germination culture, strong seedling culture, rooting culture and test-tube seedling transplantation. The method of the present invention only needs 115d to 135d to obtain the Dendrobium chrysalis seedlings; the seed germination and bud differentiation of the present invention are carried out synchronously, which simplifies the cultivation process and reduces the cultivation cost, and the seed germination rate is high and the seedling time is short, i.e. The reproduction efficiency is high, thereby improving the efficiency of the whole seedling cultivation; in addition, the test-tube seedlings cultivated by the present invention are robust and have well-developed root systems, and through domestication and hardening, the environmental adaptability is strong, thereby improving the survival rate of seedling transplanting, Thereby realize the purpose of rapid breeding of Dendrobium chrysanthemum seedlings.

The invention discloses a method for imitating wild cultivation of seedlings via Anoectochilus roxburghii protocorms, which comprises: sterilizing mature capsules of Anoectochilus roxburghii as a material; slitting the capsules; sowing seeds to a culture medium; after the seeds germinate to form protocorms, carrying out propagation culture; directly broadcasting the protocorms into a field humus-rich forest land; building a small arch shed with film; allowing protocorms to grow roots and buds in the small arch shed with certain humidity and to absorb nutrients in nature; removing the film whenseedlings grow 2-3 leaves so that imitating wild propagation of seedlings via Anoectochilus roxburghii protocorms is achieved. The method has the advantages that the growth time of Anoectochilus roxburghii seedlings is short, the survival rate is high, the regularity is good, no variation occurs, no plant growth regulator residue exists during picking, the plant stress resistance is good, the growth is fast, the yield is high, the actual problem in the production of Anoectochilus roxburghii at present is solved, the contradiction between supply and demand of Anoectochilus roxburghii can be effectively relieved, and Anoectochilus roxburghii seedlings are high in yield and quality, the benefit is good, and the cost is low.

The invention relates to a culture medium capable of inducing differentiation of protocorm of dendrobium, and belongs to the technical field of plant tissue culture. The culture medium comprises following components: KNO3, NH4NO3, KH2PO4, MgSO4.7H2O, CaCl2.2H2O, KI, H3BO3, MnSO4.4H2O, ZnSO4.7H2O, Na2MoO4.2H2O, CuSO4.5H2O, CoCl2.6H2O, Na2.EDTA, FeSO4.7H2O, inositol, glycine, thiamine hydrochloride, pyridoxine hydrochloride, niacin, cane sugar, agar, mashed banana, mashed potato, and activated carbon. The culture medium has the beneficial effects of containing no hormone and shortening the seedling growing time.

The invention belongs to the field of traditional Chinese medicinal materials, and relates to a method for synchronous production and hardening of Dendrobium candidum seedlings, in particular to a method for multiplying Dendrobium candidum tissue-cultured seedlings by using an intermittent submerged liquid culture system and hardening the seedlings in the same reactor . The invention uses aseptic dendrobium officinale seeds, protocorms and tissue culture seedlings as inoculation materials, obtains a large number of tissue culture plantlets of dendrobium officinale through inoculation and culture in a batch immersion culture system, and completes seedling hardening in the same reactor. The invention provides a method with high efficiency and low cost for the production of dendrobium officinale tissue culture seedlings.

The invention provides a cultivating method of an oncorhynchus mykiss walbaum gynoecial hybrid offspring seed. The method comprises the following steps of: using male sex hormone to induce female oncorhynchus mykiss parent fishes in a sexual maturity stage to obtain oncorhynchus mykiss false milters with male functions; and fertilizing oncorhynchus mykiss walbaum eggs and oncorhynchus mykiss false milter sperms, and incubating to obtain gynoecial hybrid oncorhynchus mykiss walbaum offspring seeds. The eyeing rate of fertilized eggs in an incubating period is more than 83 percent, the offspring seed incubating rate reaches more than 90 percent, through test, the female ratio of the obtained seeds is 100 percent, the oncorhynchus mykiss walbaum ( golden yellow) rate of the offspring seeds reaches more than 91 percent, the weight gain rate of the offspring seed is more than 114 percent after 30-day cultivation, and the survival rate reaches more than 98 percent. Compared with an offspring seed produced by mating of a purebred oncorhynchus mykiss walbaum, according to the cultivating method of the oncorhynchus mykiss walbaum gynoecial hybrid offspring seed provided by the invention, the oncorhynchus mykiss walbaum rate remains unchanged, the oncorhynchus mykiss walbaum gynoecial hybrid offspring seeds with double advantages of hybridization and gynoecious are obtained, and better economic benefit and market value are achieved.

The invention relates to a semi-lignified twig cutting and fast root taking and seedling growing method for patchouli. The method comprises the key links of scion-seed stock plant selection, scion picking, root speeding treatment, cutting medium preparation, cutting and cutting post-management. According to the method, the cutting seedlings of the patchouli can root in advance, roots can be taken fast, the cutting survival rate can reach more than 99 percent, sprouting only needs 15 days, and cutting and seedling growing can be carried out all the year round. The method is easy to operate and popularize, a large number of high-quality patchouli seedlings can be cultured in a short time, and a good foundation is laid for the development of the patchouli industry.

The invention belongs to the field of high-quality seedling cultivation and application, and relates to a method for cultivating passion fruit seedlings. According to the method, a filial generation F1 obtained by hybridizing fine-tooth passion flowers and green-fruit passion flowers with high resistance is creatively selected as a stock, tender passion fruit branches with leaf buds are selected as scions, the seedlings cultivated through the grafting method are high in stem base rot resistance, and the branches of the seedlings are luxuriant, so that the seedling renewal planting frequency inthe passion fruit variety planting process is reduced, the cultivation management cost is reduced, the service life of passion fruit plants is prolonged, the harvesting life of the fruits is prolonged, the method has important significance in promoting large-scale planting of the passion fruits, operation is easy, cost is low, and the method is suitable for mass production in agriculture; and themethod has important enlightenment for high-quality seedling cultivation of other passiflora plants.

The invention belongs to the field of cultivation and application of high-quality seedlings, and relates to a method for cultivating passion fruit seedlings. The present invention creatively selects the hybrid progeny F1 obtained by crossing Passiflora serrata with strong resistance and Passiflora viridis as the rootstock, selects young passion fruit branches with leaf buds as the scion, and uses the grafting method to cultivate the hybrid offspring F1. The seedlings have strong ability to resist stem base rot, and the branches of the seedlings are luxuriant, thereby reducing the frequency of seedling renewal and planting in the process of planting passion fruit varieties, reducing the cost of cultivation management, prolonging the life of passion fruit plants, and increasing the harvesting period of fruits. It is of great significance to promote the large-scale planting of passion fruit. It is simple to operate, low in cost, suitable for large-scale production in agriculture, and has important enlightenment for the cultivation of high-quality seedlings of other plants of the Passiflora genus.

The invention discloses an actinidia arguta greenwood cutting seedling raising method, and relates to the technical field of actinidia arguta seedling raising. The method includes the following stepsthat peat and fine sand serve as a seedbed matrix, after the peat and the fine sand are mixed to be uniform, a seedbed with the height of 5-8 cm is made, and meanwhile the seedbed is completely watered with a prepared agent solution, wherein the agent solution is prepared from 3,000-4,000 ppm of IBA (indolebutyric acid), 1,000-2,000 ppm of NAA (naphthylacetic acid) and 1,000 folds of 50% carbendazim; branches with the thicknesses of 0.2-0.4 cm are collected, the collected branches are cut into cuttings with the lengths of 2-4 cm, upper notches are located at the positions 1-1.5 cm above bud eyes, one leaf is reserved, and the leaf is cut by half; the branches are inserted into the seedbed, the bud eyes are exposed out of the surface of the matrix, and the cutting density is 750-850 per square meter;after cutting is completed, the temperature is controlled at 20-28 DEG C, the air humidity is kept at 60-90%, and the photosynthetically active radiation (PAR) is kept at 10-50 umol m<-2>s<-1>.