A kind of method of rapidly propagating Dendrobium drumsticks tissue culture seedling

A technology of dendrobium chrysalis and tissue culture seedlings, which is applied to the field of rapid propagation of dendrobium chrysalis tissue culture seedlings, can solve the problem of unsatisfactory germination rate, seedling rate, reproductive efficiency and transplanting survival rate, unfavorable high-quality seedlings of dendrobium chrysalis, Problems such as long seedling propagation cycle can improve the survival rate of transplanting, overcome the relative complexity and reduce the cost of cultivation

Active Publication Date: 2019-07-05
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although there are some reports on the rapid propagation of Dendrobium drumstick tissue culture in the existing ones, in large-scale production, there are often problems such as low embryo germination rate (less than 75%), long seedling propagation cycle (more than 8 months), transplantation, etc. Problems such as low survival rate (below 85%) are planted, which is extremely unfavorable to the obtaining of high-quality seedlings of Dendrobium drumsticks
In order to solve the problems existing in the tissue culture rapid propagation of these Dendrobium chrysalis seeds, in the Chinese patent whose application number is CN201410674752.6 and titled "a method for tissue culture rapid propagation of Dendrobium chrysalis seeds", it is disclosed that the As material, medium is used for seed germination, differentiation subculture and rooting culture to obtain seedlings; although it has established the tissue culture propagation technology of Dendrobium drumsticks, its germination rate, seedling rate, reproductive efficiency and transplanting survival In addition, in the Chinese patent whose application number is CN201410392173.2 and titled "Commercial Production Method of Dendrobium chrysalis Seedlings", it is proposed to use the pods of Dendrobium chrysalis as material, and adopt improved medium to establish the Although the aseptic and rapid propagation system of Dendrobium candidum has ideal seed germination rate (above 98%), growth cycle (155d) and transplanting survival rate (above 95%), it has the defect that the operation process is relatively complicated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Selection and disinfection of explants: The mature capsules of Dendrobium chrysalis with 195 days of artificial pollination were taken as the explants, and the capsules were first rinsed under tap water for 3 minutes, then soaked in detergent solution for 5 minutes, and then rinsed with tap water Clean, and remove the persistent perianth and fruit stalk; then put the explants into a sterilized sterile container on the ultra-clean workbench, soak in 75% alcohol for 60s, and then transfer to 0.1% HgCl 2 Soak in the solution for disinfection for 8-10 minutes, then oscillate with an ultrasonic cleaner filled with sterilized sterile water (oscillating speed of the shaker is 90r / min), clean for 8-10 minutes, take it out for later use;

[0044] Seed germination: Take the cleaned and disinfected capsules, cut the pods flat and open the pericarp to expose the seeds, pick up the seeds with tweezers and spread them evenly on the surface of the seed germination medium for cultivatio...

Embodiment 2

[0049] Selection and disinfection of explants: The mature capsules of Dendrobium chrysalis 180 days after artificial pollination were taken as the explants, and the capsules were first rinsed under tap water for 5 minutes, then soaked in detergent solution for 5 minutes, and then rinsed with tap water Clean, and remove the persistent perianth and fruit stalk; then put the explants into a sterilized sterile container on the ultra-clean workbench, soak in 75% alcohol for 60s, and then transfer to 0.1% HgCl 2 Soak in the solution for disinfection for 9 minutes, then use an ultrasonic cleaner filled with sterilized sterile water to vibrate (the shaking speed of the shaker is 90r / min) to clean for 9 minutes, and take it out for use;

[0050] Seed germination: Take the cleaned and disinfected capsules, cut the pods flat and open the pericarp to expose the seeds, pick up the seeds with tweezers and spread them evenly on the surface of the seed germination medium for cultivation; the s...

Embodiment 3

[0055] Selection and disinfection of explants: Take the mature capsules of Dendrobium chrysalis 210 days old by artificial pollination as the explants, first place the capsules under the tap water to rinse for 4 minutes, then soak them in detergent solution for 5 minutes, and then rinse them with tap water Clean, and remove the persistent perianth and fruit stalk; then put the explants into a sterilized sterile container on the ultra-clean workbench, soak in 75% alcohol for 60s, and then transfer to 0.1% HgCl 2 Soak in the solution for disinfection for 10 minutes, then use an ultrasonic cleaner filled with sterilized sterile water to vibrate (the shaking speed of the shaker is 90r / min) to clean for 10 minutes, and take it out for use;

[0056]Seed germination: Take the cleaned and disinfected capsules, cut the pods flat to open the pericarp, expose the seeds, pick the seeds with tweezers and spread them evenly on the surface of the seed germination medium for cultivation; the s...

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Abstract

The invention provides a method for rapidly propagating tissue-cultured seedlings of Dendrobium chrysalis, which uses the mature capsule of Dendrobium chrysalis as the object of explants, designs special medium formulas for each cultivation stage, and achieves the purpose of rapid propagation through seeds-protocorms-buds The method comprises the following steps: selection and disinfection of explants, seed germination culture, strong seedling culture, rooting culture and test-tube seedling transplantation. The method of the present invention only needs 115d to 135d to obtain the Dendrobium chrysalis seedlings; the seed germination and bud differentiation of the present invention are carried out synchronously, which simplifies the cultivation process and reduces the cultivation cost, and the seed germination rate is high and the seedling time is short, i.e. The reproduction efficiency is high, thereby improving the efficiency of the whole seedling cultivation; in addition, the test-tube seedlings cultivated by the present invention are robust and have well-developed root systems, and through domestication and hardening, the environmental adaptability is strong, thereby improving the survival rate of seedling transplanting, Thereby realize the purpose of rapid breeding of Dendrobium chrysanthemum seedlings.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapidly propagating tissue-cultured seedlings of Dendrobium pachyrhiza. [0003] 【Background technique】 [0004] Drumstick Dendrobium (Dendrobium chrysotoxum Lindl.) is a perennial plant of the genus Dendrobium orchidaceae, also known as Dendrobium golden bow, which has both ornamental and medicinal value, and the whole plant is edible; It has the effects of nourishing yin and nourishing the stomach, clearing heat and detoxifying, and contains anti-tumor active ingredients such as Guchaifei and Erianin. It is mostly used in the treatment of chronic pharyngitis, ophthalmic diseases, and thrombotic obliterative diseases, and the effect is very obvious. At the same time, Dendrobium drumsticks is also a kind of orchid with excellent ornamental value. Its flower raceme is golden yellow, with delicate fragrance and gorgeous It is colorful ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G24/25
CPCA01G24/00A01H4/001A01H4/008
Inventor 叶秀仙钟淮钦陈艺荃林兵罗远华
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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