Technique for breeding pyrethrum of white flower

A technology of white-flowered pyrethrum and technology, which is applied in plant regeneration, horticulture, horticultural methods, etc., and can solve the problems of immature cultivation technology, poor practicability, and low efficiency

Inactive Publication Date: 2007-02-21
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to immature culture technology, low efficiency and poor practicability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Select the young shoots that grow about 10 days after the seeds germinate of the fine varieties of Pyrethrum chrysanthemum as explants. Compound disinfection method for disinfection and sterilization of explants: rinse the explants five times with distilled water, first sterilize with 2% sodium hypochlorite solution (add 4 drops of Tween-20) for 8 minutes, and then use 0.1% mercury liter solution (add 4 drip Tween-20) for 5 minutes, rinse with sterile water 5 times.

[0025] The medium for rapid propagation of clustered buds is selected as:

[0026] MS+6-BA(0.2mg / L)+IAA(0.05mg / L)+PP 333 (0.2mg / L)+sugar (30g / L)+agar (3.5g / L).

[0027] The rooting culture medium for clustering shoots is:

[0028] 1 / 2MS+IAA(0.2mg / L)+ABT(0.05mg / L)+sugar(30g / L)+agar(3.5g / L)

[0029] Culture conditions: room temperature 20°C, light intensity: 1800Lux, light time: 15h / day. Open the bottle and practice seedlings in about 20 days.

[0030] After obtaining the complete pyrethrum test-tube s...

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Abstract

A tissue culture technique for fast reproduction of white-flower dalmatian chrysanthemum includes such steps as choosing explant, sterilizing, fast reproducing contagicus buds, rooting culture, pratising seedlings and transplanting. Three sterilizing methods, optimal culture medium and optimal transplant condition are also disclosed.

Description

technical field [0001] The invention relates to a rapid propagation technique capable of maintaining an excellent variety of the medicinal plant pyrethrum chrysanthemum. Background technique [0002] White-flowered pyrethrum (Pyrethrum cinerariifolium Trev.) is a perennial herbaceous plant of the genus Fenugreek in the family Asteraceae, and its flowers or whole plants are used as medicine. The total pyrethrins extracted from the flowers contain 6 effective insecticidal components including pyrethrins I, II, cinerin I, II, and jasmonate I, II. This is a high-efficiency natural insecticide found so far that does not pollute the environment, is non-toxic and harmless to mammals and plants, quickly knocks down insects, mosquitoes, etc., and is not easy to produce drug resistance. Due to the pollution caused by pyrethroid pesticides in the production and synthesis, as well as the disadvantages of residual toxicity after use and easy to produce drug resistance, natural pyrethrum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G1/00
Inventor 刘蓁高山林岳磊曾杨
Owner CHINA PHARM UNIV
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