The invention relates to a method for separating a raceme based on a biological enzyme, in particular to an enzymatic separation method for racemizing 1-phenylethanol compound. The technical proposal adopted in the invention is that: the enzymatic separation method for racemizing the 1-phenyl ethanol compound comprises the following steps that: the vinyl acetate used as a raw material is reacted in organic solvent under the action of the biological enzyme to prepare an R-(+)-1-phenyl ethanol compound and an S-(-)-1-phenyl ethanol compound, and the reaction formula is shown as a top right formula, wherein R is one of or the combination of two or three of hydrogen, p-halogen, o-halogen, m-halogen, p-CN, o-CN, m-CN, p-C1 to C5 alkyl groups, o-C1 to C5 alkyl groups, m-C1 to C5 alkyl groups, p-nitro group, o-nitro group, m-nitro group, p-CF3,o-CF3 and m-CF3.