21 results about "Praziquantel" patented technology

The present invention relates to a composition comprising tigolaner and, optionally endoparasiticidal agents, a method for its manufacture and its use as a medicament for controlling parasites. Compositions can include tigolaner and 1,2-isopropylideneglycerol, or the composition can include praziquantel, emodepside a solvent component, and tigolaner. The composition can be used in the treatment and / or prevention of parasite infections in animals.

The present invention relates to a composition comprising tigolaner and, optionally endoparasiticidal agents, a method for its manufacture and its use as a medicament for controlling parasites. Compositions can include tigolaner and 1,2-isopropylideneglycerol, or the composition can include praziquantel, emodepside a solvent component, and tigolaner. The composition can be used in the treatment and / or prevention of parasite infections in animals.

The invention relates to the technical field of tapeworm identification, and provides a kit for identifying five target tapeworms by using specific primers and application of the kit. All mainstream genotypes of genes of Echinococcus granulosus, Echinococcus multilocularis, Taenia vesicula, Taenia multiceps and Taenia canis are compared, and a primer pair capable of universally detecting nucleic acids of five tapeworms is obtained by taking conserved regions of the genes as diagnostic targets. The rapid detection of the five pathogens is realized by combining with a digoxin-labeled nucleic acid lateral chromatography test paper. A product obtained by isothermal amplification is detected by a nucleic acid detection test strip and then is directly observed by eyes, and the result is interpreted within 15-20 minutes, so that the operation is simple and convenient. The method provided by the invention has wide application prospects in the aspects of large-scale screening and detection of whether dogs are infected with echinococcus granulosus, echinococcus multilocularis, taenia vesicula, taenia multiceps and tapestoma dogs or not in pasturing areas, especially in areas with epidemic echinococcosis, and evaluation of the insect expelling effect of the dogs fed with praziquantel on the tapeworms.

The present application provides a milbemycin oxime praziquantel-based parasitic expelling drug, which comprises the following components in parts by mass: modified milbemycin oxime 10-30 parts, nano-coated praziquantel 20-50 parts, compound synergist 5-15 parts, flavoring agent 3-8 parts, and disintegrating agent 2-5 parts. The application of the milbemycin oxime praziquantel-based parasitic expelling drug is used for preparing a preparation for preventing or treating mixed parasitic infection of dogs and cats, wherein the parasites include tapeworms, nematodes, trematodes and Demodex. Through the integrated innovation of chemical modification (succinylated milbemycin oxime), nanotechnology (HPMC-coated praziquantel) and compound synergy (non-ban Tai'er-beta cyclodextrin), the comprehensive advantages of broad-spectrum parasitic expelling, long-acting stability, safety and low toxicity are realized, and the pain points such as poor palatability, high metabolic burden and weak environmental adaptability in the prior art are solved, and the application is suitable for the prevention and treatment of mixed parasitic infection of dogs and cats.

The invention relates to the technical field of biochemical engineering and medicinal chemistry, and provides a method for improving the resolution efficiency of a praziquantel racemoid by an enzyme method, which comprises the following steps: S1, preparing oxidase, selectively oxidizing dextropraziquantel by the oxidase, and basically not oxidizing levo praziquantel; s2, contacting oxidase with the racemic praziquantel mixture in a Tris-HCl buffer system with the pH value of 7.5-8.5, adding Fe as a cofactor, and reacting at the temperature of 35-39 DEG C for 4-6 hours; and S3, separating and purifying the reaction mixture obtained in the step S2 to obtain the high-purity levo-praziquantel. The problem of low resolution efficiency of the praziquantel racemate is successfully solved through an enzymatic resolution technology with high efficiency, strong specificity and mild conditions.

The invention provides a method for detecting the content of compound selamectin drops, which adopts high performance liquid chromatography for detection, and comprises the following specific steps: sequentially preparing a blank solution, a blank auxiliary material solution, a contrast solution and a test solution, sequentially and respectively injecting the blank solution, the blank auxiliary material solution, the contrast solution and the test solution into a high performance liquid chromatograph, an octadecyl silane bonded silica gel column is used as a chromatographic column, a formic acid solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, and gradient elution is carried out. According to the method disclosed by the invention, the three active ingredients, namely the selamectin, the fluralan and the praziquantel, in the compound selamectin drops can be effectively detected, the quality detection result is accurate and reliable, and the three active ingredients in the compound selamectin drops can be accurately quantified, so that the product quality of the compound selamectin drops can be better controlled.

The invention discloses a formula and a preparation method of a stomach-invigorating parasite-expelling tablet for livestock. The parasite expelling tablet is formed by compounding and tabletting parasite expelling core enteric particles A and stomach invigorating and relieving quick-release particles B. The particles A contain praziquantel and levamisole or salts thereof, and are coated with enteric materials, so that the parasite expelling activity is limited in gastric segment release and is quickly released in intestinal segment; the particles B contain a cortex meliae extract, a fructus quisqualis extract, pericarpium citri reticulatae, fried malt and fructus crataegi, are compounded with mucous membrane protection or adsorption stimulation components such as sodium alginate / pectin / montmorillonite and the like, and are subjected to post-treatment adsorption through essence, so that the taste covering stability is improved. The preparation method comprises the following steps: plant extraction, wet granulation in two ways, enteric coating of particles A, essence post-treatment of particles B, and compounding, lubricating and tabletting. According to the scheme, the insect expelling effect is guaranteed, gastrointestinal stimulation is reduced, the voluntary ingestion rate and administration compliance are improved, and the composition is suitable for dogs, cats and livestock to expel insects by oral administration.

The invention discloses a composition capable of efficiently killing a greedy meimeria disease and application of the composition. The composition is prepared from 0.1 mg / mL to 0.9 mg / mL of astragalus polysaccharide, 0.005 mg / mL to 0.015 mg / mL of carvacrol, 0.05 mg / mL to 0.15 mg / mL of praziquantel and 0.4 mg / mL to 1.5 mg / mL of herba epimedii. The invention finds that after the astragalus polysaccharide, carvacrol, praziquantel and epimedium are combined for use, a remarkable synergistic effect is generated, and the effect of killing the greedy Michamei insect can be remarkably improved within the same time at the dosage which is far lower than the effective concentration of each single component of the astragalus polysaccharide, the carvacrol, the praziquantel and the epimedium. The dosage of four drugs is greatly reduced, the method has the advantages of being efficient, low in cost, capable of reducing drug residues and the like, and a new scheme can be provided for prevention and treatment of the greedy Michameiasis of the large yellow croakers.

The present invention is aimed at a pharmaceutical formulation or composition and a method of manufacture or production thereof, wherein the pharmaceutical composition includes fluralaner, moxidectin and praziquantel for the treatment of parasitic infestations in animals, wherein the composition may be in tablet form, more particularly as a chewable tablet and more preferably as a soft chewable tablet that manages to mask the bitter taste of the active ingredient praziquantel based on an association of bitter taste receptor blockers.

The invention provides a rapid detection method for non-pralazone illegally added in food, and belongs to the technical field of medicine detection. The method comprises the following steps: respectively carrying out secondary extraction on a food sample by adopting a strong polar extraction agent and a non-polar extraction agent to obtain extracted supernate; removing the non-polar extraction agent in the supernate by adopting a nitrogen blowing method; eluting the supernate subjected to nitrogen blowing treatment by adopting a strong polar solvent to prepare a to-be-detected solution; and sequentially adding the metal sol and the inorganic salt flocculant into the to-be-detected solution, uniformly mixing, and detecting by using a Raman spectrometer. The detection method provided by the invention has the advantages of high sensitivity, low detection limit and low cost, and can realize rapid detection of non-pralazone illegally added in food.

A veterinary extruded soft chew has as active pharmaceutical ingredients (APIs), milbemycin oxime or one of its salts, and praziquantel or one of its salts. The APIs are uniformly dispersed in a matrix having one or more fillers, a polyethylene glycol (PEG), a non-animal based palatant, a disintegrant, a humectant, an oil, water, and preservatives. Neither the milbemycin oxime or one of its salts, nor the praziquantel or one of its salts is coated, granulated or physically separated from the other APIs within the matrix. More importantly, the soft chew does not comprise any palatant of animal origin.

The invention discloses a praziquantel derivative PT-28 as well as a preparation method and application thereof. According to the praziquantel derivative PT-28, a thymol precursor and amino-praziquantel react to generate the derivative. The praziquantel derivative PT-28 can be used as a drug for resisting third-generation insect diseases, and compared with the national standard anti-parasitic drug praziquantel, the safety index-therapeutic index (TI value) of the drug is improved by 8.89 times. The compound has the characteristics of being safe to fishes, low in toxicity and good in killing effect on third-generation insects, and has wide application prospects in development of novel insect-resistant fishery drugs.

Preparation method for a compound as shown in formula (I) and levo-praziquantel. A racemate of ester of the compound of formula (I) is used as a substrate, and reaction is performed under the catalysis of immobilized lipase to produce the compound of formula (I); a circulating fluidized bed reactor is used, the circulating fluidized bed reactor includes an external circulation system and a reaction column, the immobilized lipase is arranged in the reaction column, the substrate solution is circulated many times between the external circulation system and the reaction column, the reaction is performed in the reaction column. The preparation of levo-praziquantel includes the described preparation steps of the formula (I). The preparation method of formula (I) can significantly improve the utilization rate of enzyme and reduce the use ratio of enzyme / substrate, and has mild reaction conditions, strong stereoselectivity, high reaction efficiency.

The invention discloses 10-[7-(2-decyloxy) coumarin] praziquantel and a preparation method and application thereof.The praziquantel derivative 10-[7-(2-decyloxy) coumarin] praziquantel is obtained by connecting coumarin to the 10-site of an aromatic ring through an alkyl chain on the basis of the structure of praziquantel, and after insect-resistant evaluation and safety evaluation, the 10-[7-(2-decyloxy) coumarin] praziquantel derivative can be used for preparing the praziquantel derivative. The 10-[7-(2-decyloxy) coumarin] praziquantel provided by the invention can be used as a drug for resisting monocoloniasis, and shows activity and safety superior to those of praziquantel.

A veterinary extruded soft chew has as active pharmaceutical ingredients (APIs), milbemycin oxime or one of its salts, and praziquantel or one of its salts. The APIs are uniformly dispersed in a matrix having one or more fillers, a polyethylene glycol (PEG), a non-animal based palatant, a disintegrant, a humectant, an oil, water, and preservatives. Neither the milbemycin oxime or one of its salts, nor the praziquantel or one of its salts is coated, granulated or physically separated from the other APIs within the matrix. More importantly, the soft chew does not comprise any palatant of animal origin.

The present disclosure provides novel chemotypes, pharmaceutical compositions thereof, and method of use thereof for treating parasitic infection, including infection caused by parasitic flatworms. The present compounds may be useful as alternatives to praziquantel and may have improved activities against parasitic infections, particularly parasitic flatworm infections.

The present invention is aimed at a pharmaceutical formulation or composition and a method of manufacture or production thereof, wherein the pharmaceutical composition includes fluralaner, moxidectin and praziquantel for the treatment of parasitic infestations in animals, wherein the composition may be in tablet form, more particularly as a chewable tablet and more preferably as a soft chewable tablet that manages to mask the bitter taste of the active ingredient praziquantel based on an association of bitter taste receptor blockers.

The invention discloses a praziquantel derivative PC-6 and a preparation method and application thereof.The praziquantel derivative PC-6 is obtained by introducing an active group coumarin into a praziquantel structure through a pharmacophore fusion strategy, and the praziquantel derivative PC-6 has the application advantages that the insect-resistant efficacy of praziquantel is improved, and parasite drug resistance is reduced. The 48-hour median inhibitory concentration (EC50) of the praziquantel derivative PC-6 to third-generation microforest worms is 1.94 mu M, and the pesticide effect of the praziquantel derivative PC-6 is improved by 3.99 times compared with that of praziquantel; the 96h median lethal concentration (LC50) of zebra fish is 70.49 mu M, and the toxicity is reduced by 3.20 times compared with praziquantel; the therapeutic index (TI) is 36.34, which is 12.78 times that of praziquantel. According to the invention, a pharmacophore fusion strategy is utilized to synthesize a high-efficiency and low-toxicity anti-homoparagonimus drug, the production cost is low, the use method is simple, and the drug has important development and application prospects.

This invention relates to the fields of medicinal chemistry and biotechnology, and proposes a method for improving the resolution efficiency of drugs used to treat echinococcosis, comprising the following steps: Step 1: Praziquantel is reacted with a designed enzyme in a buffer solution containing Fe²⁺ for enzymatic reaction; Step 2: After removing the enzyme protein, the pH is adjusted to 1.0 for crystallization; Step 3: The praziquantel enzymatic reaction product detection solution is freeze-dried in a carbon dioxide incubator until the total amount of praziquantel and its derivatives is 350 mg / L, and then the pH is adjusted to 1.0 with 2 mol / L hydrochloric acid under ice bath conditions to obtain precipitated levamisole crystals. This invention achieves selective catalytic modification of dextrorotatory praziquantel using a genetically engineered specific enzyme while leaving levamisole almost unaffected, significantly improving the resolution efficiency of praziquantel.

Disclosed is a use of a composition comprising uridine or a derivative thereof and praziquantel in preparation of a medicament for preventing or treating liver injury, and in particular in preparation of a medicament for treating hepatic fibrosis caused by schistosomiasis.

The application discloses a method for detecting the content of fepronil, methoprene, praziquantel and moxidine, and the method comprises the following steps: detecting the content of fepronil, methoprene, praziquantel and moxidine in a compound preparation by using high performance liquid chromatography; and the chromatographic conditions for gradient elution during the detection are as follows: using a mixed solution of water, acetonitrile, methanol and glacial acetic acid as mobile phase A, using a mixed solution of acetonitrile, methanol and glacial acetic acid as mobile phase B, the flow rate is 0.85-1.25 ml / min, the column temperature is 20-30 DEG C, the injection amount is 15-30 muL, the detection wavelength is 230-250 nm, and the theoretical plate number is not less than 5000 according to the main peak. The method can simultaneously detect the content of fepronil, methoprene, praziquantel and moxidine, has good specificity, strong stability, good reproducibility and accurate detection result; the gradient elution mode, low column temperature and low flow rate are used, the damage to the chromatographic column and the instrument is reduced, the use amount of reagents is reduced, and the detection cost is greatly saved.