33results about How to "Improve the survival rate of hardened seedlings" patented technology

A tissue culture method for new pteris fern includes such steps as obtain aseptic material, primary culture with the culture medium containing MS, 6-BA, NAA and AC, reproduction culture with the culture medium containing MS, 6-BA and AC, rooting culture with the culture medium containing 1/2 MS, NAA and IB, and intelligent culture of seedlings.

The invention relates to a method for domesticating hardening seedlings of tissue culture seedlings of Nandina domestica firepower. The method is characterized by comprising the following steps of establishing a hardening seedling greenhouse; screening and treating a hardening seedling matrix; managing the tissue culture seedlings before the tissue culture seedlings are bottled out; treating the tissue culture seedlings after the tissue culture seedlings are bottled out; transplanting the hardening seedlings of the tissue culture seedlings; managing the tissue culture seedlings after the hardening seedlings of the tissue culture seedlings are planted; and domesticating the hardening seedlings of the tissue culture seedlings for four months to obtain qualified domestication seedlings of the Nandina domestica firepower. By means of the method for domesticating the hardening seedlings of the tissue culture seedlings of the Nandina domestica firepower, surviving rates of the domestication seedlings are increased form 58% of traditional surviving rates to 83.5% so that the surviving rates of the domestication seedlings of the tissue culture seedlings of the Nandina domestica firepower are greatly increased, the tissue culture seedlings which are obtained by four month domestication of the domestication seedlings can basically adapt to growth environment of the greenhouse, root systems of plants are thick, new twigs are good, stalks are thick, and the surviving rates of the transplanted domestication seedlings can reach 95.8%.

An open plant tissue culture and factory rapid propagation method disclosed in the invention, is characterized by: using a transparent container, dispensing the boiled medium containing antibacterial agent Shannon 1 with different concentrations into a culture vessel to prepare a medium, sterilizing the inoculation room with alcohol for dust control and ultraviolet irradiation, cleaning the surface of the inoculation bench and hands with alcohol, opening a bottle cap, inoculating the plant to the medium by using an inoculator, and then sealing. The invention has the beneficial effects that: the culture container has a broad range of choice, a plastic cup, plastic casing with autoclaving resistance can be used as the culture container; the medium has no loss of components without autoclaving, hormone types have a broad range of choice; the inoculation can be directly carried out in an open and bacterial environment without a clean bench, the inoculation speed is 3-5 times traditional inoculation; by culturing in the open and bacterial greenhouse, developing from a heterotrophic mode of traditional tissue culture to a mode containing autotrophism and heterotrophism, tissue culture plants grow health and strong, the survival rate of the hardening seedling in the mode containing autotrophism and heterotrophism is significantly higher than that of traditional mode.

The invention belongs to the technical field of apple virus-free seedling factory propagation, and especially relates to an apple tissue culture seedling two-step transplanting method with a high survival rate. The method is characterized by comprising the following steps: (1) rooting culture; (2) indoor domestication; (3) waterweed transplantation; (4) transplantation with a waterweed matrix; and(5) field transplantation. The method has the advantages that the operation and technology are simple, the cost is low, the domestication time is short and only lasts for 4 days, and the domestication time is shortened by 10 to 13 days. After first transplantation, the tissue culture seedling autotrophy root systems grow fast, in the 15th day, the length of more than 90% of tissue culture seedling autotrophy root systems can reach 1.5 cm or more; moreover, waterweed has a good moisturizing performance, the temperature and humidity in a greenhouse are easy to control; the survival rate of transplantation is high, the transplantation survival rate of the apple tissue culture seedling can reach 95.6% or more; the roots are not damaged in two transplantations; for the field transplantation, seedling recovery is not needed, the seedlings grow fast and can be grafted in the current year, the application range of the method is wide, and the method is suitable for most apple species.

The invention relates to a seedling hardening and domestication method in greenhouse for forest tree tissue culture seedlings. The method is characterized by comprising following steps: (1) seedling hardening greenhouse preparation; (2) seedling hardening management of tissue culture seedlings before bottle out; (3) cultivation substrate and container preparation; (4) tissue culture seedling transplanting; (5) domestication management after transplanting; (6) domestication time control after transplanting. According to the seedling hardening and domestication method in greenhouse for forest tree tissue culture seedlings, a uniform and stable domestication environment is formed with white transparent nursery pot used for butterfly orchid production and plates of the corresponding size. Compared with the prior art, the method is simple and efficient; the plates and the nursery pots can be reused, which greatly reduce production cost and can be promoted in large scale; according to the measure integrated effect before, during and after the seedling hardening; the time for seedling hardening after tissue culture seedling transplanting in greenhouse can be shortened by at least one month; the survival rate of seedling hardening is increased by 5 to 15 percentage; the manpower and material cost is reduced by about 30%; the over ground part of the tissue culture seedlings is increased by 30 to 50% compared with seedlings planted by regular methods. The plant grows well.

The invention discloses a cultivation method of chili disease-resistant homozygote. The method includes steps: culturing and identifying tissue: utilizing a conventional culture medium, using trehalose to replace sucrose, adding lycium ruthenicum juice filtrate nutritional ingredients to prepare a specific culture medium formula, and performing explant selection, explant treatment, anther culture,complete plant acquisition, colchicine treatment and ploidy identification on chili to obtain homozygote seedlings; utilizing molecular marker technology for virus disease resistant molecular markerscreening to obtain virus disease resistant homozygote plants. The method can remarkably shorten screening period and quickly acquire the chili disease-resistant homozygote, is simple to operate, lowin cost and easy for popularization, can be directly applied in production and has good economic benefit and social benefit,, and anther cultured seedlings are high in survival rate and stress resistance after being transplanted.

The invention discloses a matrix used for exercising seedlings of blueberry and a seedling exercising method. The seedling exercising matrix comprises a water culture matrix and a soil culture matrix,wherein the water culture matrix comprises 1 part by mass of a bactericidal solvent, 1 part by mass of an acid adjusting solvent, 1 part by mass of a fertility increasing solvent, and 1 part by massof a rooting solvent, and the soil culture matrix comprises 1,200-1,300 parts by weight of mixed soil, 0.3-0.4 part by weight of terminalia catappa leaves, 0.3-0.35 part by weight of sulfur, and 0.2-0.3 part by weight of a compost quick decomposing agent. The seedling exercising method comprises the following steps: taking out tissue culture seedlings in a culture bottle, wrapping the seedlings bymosses, placing the wrapped seedlings into a seedling exercising plug tray; placing the seedling exercising plug tray into a pond containing the water culture matrix, and exercising the seedlings for28-35 days; and placing the seedling exercising plug tray onto the soil culture matrix, and exercising the seedlings for 4-6 months. The seedling exercising method provided by the invention can reduce the probability of diseases of the blueberry, and significantly improve the survival rate.

The invention mainly relates to the field of planting technology, and discloses a method for promoting the differentiation of Trichosanthes mellifera seedlings, including: selection and treatment of explants, preparation of medium, inoculation of explants, induction of differentiation and cultivation; Induced differentiation during basket tissue culture, after 16 days of culture in B5 medium, yellow-green cell protrusions began to appear, and then differentiated bud clusters appeared, which significantly shortened the time for induction of differentiation, improved planting efficiency, and increased economic income by 16.2%; As an explant, it will not cause damage to the Trichosanthes plant, does not affect the growth and results of Trichosanthes, and the source of young leaves is extensive, and tissue culture can be carried out at any time, and the planting efficiency is significantly improved; Salt water, acetic acid solution and nutrient solution treatment, avoid using too much disinfectant for repeated disinfection, save costs, and allow explants to continue absorbing and accumulating nutrients in a slightly acidic environment, speeding up the process of inducing differentiation of explants.

The invention relates to a low temperature seedling hardening method for a psammosilene tunicoides tissue culture seedling. The method comprises the steps: transferring psammosilene tunicoides bottled seedling into a seedling hardening culture chamber, and fumigating in a closed manner for 20 min by virtue of potassium permanganate and formaldehyde; culturing the tissue culture seedling for 7 days in an environment with good ventilation at the ambient temperature of 16 to 18 DEG C, the light intensity of 1800 to 2000 Lx and the lighting time of 12 h/d; adjusting the temperature to 11 to 13 DEG C, adjusting the light intensity to 1500 to 1800 Lx, continuously culturing for 14 days, then taking out the psammosilene tunicoides tissue culture seedling by virtue of tweezers, soaking in a 800-time carbendazim disinfectant for 2 h, air-drying, and then transplanting the psammosilene tunicoides tissue culture seedling into a seedling hardening matrix. On the basis that the content of soluble sugar of plants is increased in a low temperature environment, the water content is reduced, the enzyme activity is reduced and air holes are closed, the stress resistance of the plant is finally improved, the rejuvenation period is obviously shortened after the seedling is transplanted, and the probability of the plant infected by root rot and stem rot is reduced, so that the transplanting survival rate is increased to 95 percent or more, and the seedling hardening transplanting survival rate is greatly increased.