33results about How to "Improve the survival rate of hardened seedlings" patented technology

The invention discloses a method for establishing a general maize regeneration system. The method comprises the following steps of: inoculating different maize inbred line embryos onto induction mediums with different hormone combinations, transferring an appropriate induced embryoid onto a differential medium to carry out high frequency differentiation on regenerated seedlings, transferring the regenerated seedlings onto a rooting medium to carrying out rooting induction so as to obtain a regenerated plant, wherein the formula of the induction medium comprises N6 marcoelement, B5 microelement, B5 vitamin, riboflavin (0.04mg / L), folic acid (0.04mg / L), nicotinic acid (0.1mg / L), D-calcium pantothenate (0.1mg / L), methyl benzoic acid (0.04mg / L), cane sugar (50g / L), 2.4-D (0.4mg / L) and 6-BA (5mg / L). The method disclosed by the invention has the characteristics of good generality to different maize inbred lines and shorter time for obtaining the regenerated plant and has important application value.

A tissue culture method for new pteris fern includes such steps as obtain aseptic material, primary culture with the culture medium containing MS, 6-BA, NAA and AC, reproduction culture with the culture medium containing MS, 6-BA and AC, rooting culture with the culture medium containing 1 / 2 MS, NAA and IB, and intelligent culture of seedlings.

The invention discloses an industrial tissue culture and rapid propagation technology for tamarix chinensis. Unified tissue culture and rapid propagation breeding of various tamarix chinensis is realized finally through explant selection, establishment of a disinfection system, multiplication culture, combination of strong seedling culture and rooting culture as well as exercising seedling optimization, several key nodes including initiation rate, quantity of cluster buds on stems, rooting rate, root number, root length and survival rate of exercising seedlings for tissue culture breeding are optimized and controlled, the survival rate of the exercising seedlings with higher consistency is obtained finally, the effects of high initiation rate, many cluster buds, high survival rate and high reproduction coefficient are realized, great convenience and technical support are provided for industrial breeding of the tamarix chinensis, and the technology is quite suitable for industrial breeding production.

The invention discloses a subculture method for tissue culture seedlings of cercis giganter lines. The subculture method is characterized by comprising the following steps: 1, obtaining sterile explants; 2, carrying out subculture multiplication on the cercis giganter lines; 3, carrying out rooting culture on the cercis giganter lines; 4, controlling subculture and rooting culture conditions of the cercis giganter lines; and 5, hardening and transplanting the tissue culture seedlings of the cercis giganter lines. The multiplication method for the tissue culture seedlings of the cercis giganter lines disclosed by the invention has the following advantages that (1) the multiplication coefficient is high and the average multiplication coefficient reaches above 5; (2) the rooting rate is high and the maximum rooting rate can reach 98%; and (3) the survival rate of hardened seedlings is high and averagely above 90%.

The invention discloses a tissue culture method for ilex attenuata 'Sunny Foster'. The method includes the following steps of pretreatment: taking stems of terminal buds or axillary buds of the ilex attenuata 'Sunny Foster', and conducting disinfection and sterilization treatment to obtain a disinfected and sterilized explant; primary culture: inoculating the disinfected and sterilized explant into a primary culture medium for primary culture to obtain axillary buds; proliferation culture: inoculating the axillary buds into a proliferation culture medium for proliferation culture to obtain proliferative seedlings; rooting culture: inoculating single plants of the proliferative seedlings into a rooting culture medium for rooting culture to obtain rooting seedlings. The tissue culture methodhas high seedling hardening survival rate, high reproduction coefficient, simple and convenient tissue culture process and short reproduction time, and is not limited by seasons, and the market demand for the ilex attenuata 'Sunny Foster' is met.

The invention belongs to the technical field of apple virus-free seedling factory propagation, and especially relates to an apple tissue culture seedling two-step transplanting method with a high survival rate. The method is characterized by comprising the following steps: (1) rooting culture; (2) indoor domestication; (3) waterweed transplantation; (4) transplantation with a waterweed matrix; and(5) field transplantation. The method has the advantages that the operation and technology are simple, the cost is low, the domestication time is short and only lasts for 4 days, and the domestication time is shortened by 10 to 13 days. After first transplantation, the tissue culture seedling autotrophy root systems grow fast, in the 15th day, the length of more than 90% of tissue culture seedling autotrophy root systems can reach 1.5 cm or more; moreover, waterweed has a good moisturizing performance, the temperature and humidity in a greenhouse are easy to control; the survival rate of transplantation is high, the transplantation survival rate of the apple tissue culture seedling can reach 95.6% or more; the roots are not damaged in two transplantations; for the field transplantation, seedling recovery is not needed, the seedlings grow fast and can be grafted in the current year, the application range of the method is wide, and the method is suitable for most apple species.

The invention discloses a rapid breeding method of a saline and alkaline resistant poplar. The method comprises the steps including preparation of materials for breeding, collection and storage of a cutting slip, processing of a slip, cutting and management, seedling hardening and transplanting. The cutting slip and the slip are processed by specific cultivation matrix and treatment fluid before cutting. The method is simple in operation and low in cost, the cutting survival rate can reach 98% or higher, the survival rate after cutting and transplantation is high, stress resistance is high, the method can be applied to production directly, and the economical and social values of the method are high.

The invention discloses a method for cultivating chili disease-resistant homozygotes. The method includes a tissue culture identification step and an anti-viral disease screening step. The tissue culture identification is based on conventional culture medium, using trehalose instead of sucrose and adding black wolfberry juice The nutrient composition of the filtrate was formulated to prepare a specific medium formula for the selection of explants, explant treatment, anther culture, acquisition of complete plants, colchicine treatment and ploidy identification of peppers to obtain homozygous seedlings; and use molecular The marker technology is used to screen anti-viral disease molecular markers to obtain anti-viral disease homozygous plants. The method of the present invention can significantly shorten the screening period, and quickly obtain homozygous anti-viral diseases of pepper; and the method is simple in operation, low in cost, high in survival rate after transplanting flower seedlings, strong in stress resistance, easy to popularize, and can be directly applied to production , have good economic and social benefits.

The invention belongs to the field of traditional Chinese medicinal materials, and relates to a method for synchronous production and hardening of Dendrobium candidum seedlings, in particular to a method for multiplying Dendrobium candidum tissue-cultured seedlings by using an intermittent submerged liquid culture system and hardening the seedlings in the same reactor . The invention uses aseptic dendrobium officinale seeds, protocorms and tissue culture seedlings as inoculation materials, obtains a large number of tissue culture plantlets of dendrobium officinale through inoculation and culture in a batch immersion culture system, and completes seedling hardening in the same reactor. The invention provides a method with high efficiency and low cost for the production of dendrobium officinale tissue culture seedlings.

The invention relates to a method for domesticating hardening seedlings of tissue culture seedlings of Nandina domestica firepower. The method is characterized by comprising the following steps of establishing a hardening seedling greenhouse; screening and treating a hardening seedling matrix; managing the tissue culture seedlings before the tissue culture seedlings are bottled out; treating the tissue culture seedlings after the tissue culture seedlings are bottled out; transplanting the hardening seedlings of the tissue culture seedlings; managing the tissue culture seedlings after the hardening seedlings of the tissue culture seedlings are planted; and domesticating the hardening seedlings of the tissue culture seedlings for four months to obtain qualified domestication seedlings of the Nandina domestica firepower. By means of the method for domesticating the hardening seedlings of the tissue culture seedlings of the Nandina domestica firepower, surviving rates of the domestication seedlings are increased form 58% of traditional surviving rates to 83.5% so that the surviving rates of the domestication seedlings of the tissue culture seedlings of the Nandina domestica firepower are greatly increased, the tissue culture seedlings which are obtained by four month domestication of the domestication seedlings can basically adapt to growth environment of the greenhouse, root systems of plants are thick, new twigs are good, stalks are thick, and the surviving rates of the transplanted domestication seedlings can reach 95.8%.

The invention discloses a cutting seedling nutrient solution of lonicera edulis twigs as well as a preparation method and an application method thereof. The cutting seedling nutrient solution includes the following components: 180-220 ml / L honey, 0.9-1.1 ml / L liquid organosilicone, 90-110 ml / L edible vinegar, 45-55 ml / L red orchid extracting solution, 360-440 mg / L pimacol, 36-44 mg / L indolebutyric acid, 9-11 mg / L dichlorphenoxyacetic acid (2, 4-D), 0.9-1.1 g / L potassium dihydrogen phosphate, 0.09-0.11 mg / L iron, 0.045-0.055 mg / L born, 0.036-0.044 mg / L manganese, 0.036-0.044 mg / L copper and 0.027-0.033 mg / L zinc, the cutting seedling nutrient solution disclosed by the invention can be used for preventing decay of a lower notch during a seedling breeding process of the lonicera edulis twigs and promoting quick rooting of an in vitro material.

The invention relates to the technical field of agricultural science, and especially relates to red-leaf pistacia tissue culture seedling hardening media and a seedling hardening method. The seedling hardening media comprise a seedbed seedling hardening medium and a container seedling hardening medium. The seedbed seedling hardening medium comprises peat and perlite with a volume ratio of 2:1. The container seedling hardening medium comprises peat, perlite and vermiculite with a volume ratio of 2:1:1. According to the invention, medium formulas are optimized. With the seedbed seedling hardening medium, the container seedling hardening medium and the seedling hardening process, a seedling hardening survival rate is increased from traditionally 50% to approximately 75%, such that red-leaf pistacia tissue culture seedling hardening survival rate is greatly improved.

The invention discloses a rapid seedling hardening method for tissue culture seedlings of rhododendron lapponicum. The method comprises acclimation of tissue culture rooting seedlings in a bottle, transplantation, environment control after transplantation, fertilization management and pest control, wherein the environment control is implemented as follows: a small arch shed is put up below a sunshade net arch structure set up in a large shed, the top surface of the small arch shed is covered with a sunshade net, and a hole tray is put in the small arch shed; the humidity of the small arch shed is kept at 80%-85%, the temperature in the daytime is kept 25 DEG C-30 DEG C, the temperature at night is kept not lower than 5 DEG C, and moisture is controlled; fertilization management and pest control are implemented as follows: after the tissue culture seedlings are transplanted to the hole tray for 30 days, a leaf fertilizer is applied once every two weeks, chlorothalonil or carbendazim is sprayed once every 15 days and sprayed for 3-4 times in total, and imidacloprid is sprayed once every week at the initial stage of aphids in spring and sprayed for 3-4 times in total. By means of the method, the transition time of the tissue culture seedlings of the rhododendron lapponicum is shortened, the survival rate of hardened seedlings is up to 90% or higher, the seedlings grow healthy and strong, a standard seedling hardening technical scheme is provided, and the method is suitable for industrial seedling hardening.