22results about How to "Small variation" patented technology

The invention provides an exposure system, a photoetching machine and an exposure control method, and relates to the technical field of photoetching machines. A temperature sensor in an exposure control device in the exposure system detects the temperature of a light intensity sensor to obtain the real-time temperature of the light intensity sensor after a shutter is opened; a temperature controller in the exposure control device obtains the real-time temperature and determines the actual light intensity energy corresponding to the real-time temperature according to the real-time temperature and the corresponding relation between the preset temperature and the light intensity energy value; the temperature controller is used for controlling a semiconductor cooling sheet in the exposure control device to cool the light intensity sensor based on the real-time temperature; and the dose calculation equipment obtains the actual light intensity energy, calculates the exposure dose based on the actual light intensity energy, and performs exposure control based on the exposure dose. Therefore, the influence of temperature drift caused by rapid temperature rise on the measurement accuracy of the light intensity sensor is overcome from a hardware structure, accurate actual light intensity energy is obtained, and the calculation of the exposure dose is more accurate.

The embodiment of the invention relates to a method for determining the biological activity of recombinant human endostatin. The method comprises the following steps: carrying out mixed culture on a subculture cell which is subjected to L929 cell culture subculture and is used as an effector cell and recombinant human endostatin to be detected; cracking the cells subjected to mixed culture to obtain a lysis solution; the method comprises the following steps of: preparing a lysate, measuring the absorbance of the lysate, performing mixed culture on the basis of a measured result, a human endostatin standard substance and effector cells, and obtaining the biological activity of the recombinant human endostatin to be measured by utilizing a relative biological activity method. The biological activity of the recombinant human endostatin to be detected can be obtained, and the detection result is reliable.

The invention relates to a cross-instrument intensity correction method and system based on liquid chromatography-mass spectrometry, and the method comprises the steps: constructing a first correction curve based on mass anchor points composed of chemical standard substances with concentration gradients, and correcting the intensity of data collected by the same instrument to a similar level; and further constructing a second correction curve based on the concentration gradient sample, wherein the second correction curve is used for correcting the intensity of data collected by different instruments to a similar level. Wherein a kit consisting of chemical standard substances is designed for mass anchor points, the chemical standard substances have concentration gradient differences in multiple relation, and the types and the quantity of the chemical standard substances can be selected according to the actual condition of a sample to be detected; the construction of the concentration gradient sample comprises the following steps: diluting a quality control sample (QC) formed by mixing all samples to be detected into different concentration gradients, and adding the chemical standard substance. The indexes such as the relative standard deviation (RSD) of the corrected characteristic intensity are comprehensively improved, and the problem of data comparability of different batches, different instruments and different experimental centers can be effectively solved.

A method for cryopreservation and recovery of larch olgensis embryogenic callus relates to the biotechnology field of forest breeding, and comprises the following steps: selecting an embryogenic cell line induced by immature seeds of larch olgensis, and subculturing the embryogenic cell line for 3-5 days to obtain embryogenic callus as a standby material; carrying out gradient pre-culture at the molar concentrations of 0.2 mol / L and 0.4 mol / L in sequence, wherein the culture is carried out at each concentration for 20-48 hours; after pre-culture, putting the embryogenic callus into a freezing tube, and adding a sterile freezing protection solution; and putting the freezing pipe into a programmed cooling box for programmed cooling, and immediately putting the freezing pipe into a liquid nitrogen biological container for storage. The method disclosed by the invention is simple and convenient in operation steps and obvious in effect, the subculture age limit of the larix olgensis embryonic callus is slowed down, and relatively good cell viability and development potential in the initial stage of induction are maintained, so that the occurrence of variation is effectively reduced, manpower and material resources consumed by long-term subculture materials can be saved, and the production cost is reduced. The method is of great significance to theoretical and practical application research of larix olgensis.

Provided are a magnetostrictive member having a high magnetostriction constant and a high parallel magnetostriction amount and small variations in the magnetostriction constant and the parallel magnetostriction amount among members and a method for manufacturing such a magnetostrictive member. The magnetostrictive member is formed of a single crystal of an iron-based alloy having magnetostrictive characteristics, is a plate-like body having a long-side direction and a short-side direction, and has a lattice constant of a <100> orientation in the short-side direction larger than a lattice constant of a <100> orientation in the long-side direction.

The invention relates to the technical field of batteries, in particular to a negative electrode material preparation method based on a negative electrode aged hard carbon material, which comprises the following steps: firstly, crushing and grading an aged hard carbon precursor to obtain particles with a preset particle size; the preparation method comprises the following steps: performing pretreatment such as acid washing, alkali washing, water washing and ethanol washing, mixing a purified precursor with a coating material in proportion to form a uniform coating layer, pre-sintering in a protective atmosphere, and preliminarily stabilizing a material structure by controlling parameters such as a heating rate; and carrying out specific surface area detection on the pre-sintered primary product, and dynamically adjusting pre-sintering process parameters according to the specific surface area detection to ensure that the pore structure of the material reaches the standard. And performing high-temperature carbonization treatment on the material meeting the standard so as to repair the carbon layer structure and improve the conductivity. And finally, performing quality judgment by detecting the first coulombic efficiency of a finished product. According to the invention, the first coulombic efficiency, the specific capacity and the cycling stability of the regenerated hard carbon material are improved, and the recycling of the aging material is realized.

The invention relates to the technical field of cell culture detection, and specifically discloses a method and system for in vitro culture detection of mesenchymal stem cells and hematopoietic stem cells. The method comprises: analyzing a cell culture environment and culture medium components, determining a reference evaluation value for cell culture detection, judging whether the culture of mesenchymal stem cells and hematopoietic stem cells is qualified, analyzing unqualified mesenchymal stem cells and hematopoietic stem cells to obtain characteristic values ​​of mesenchymal stem cells and hematopoietic stem cells, and determining an adjustment plan for the culture of unqualified mesenchymal stem cells and hematopoietic stem cells based on the characteristic values ​​of the mesenchymal stem cells and the hematopoietic stem cells. The invention solves the problems that traditional culture of mesenchymal stem cells and hematopoietic stem cells lacks comprehensive and unified culture and detection standards, and that the culture adjustment plan has insufficient adaptability, is helpful to maintain the healthy state and function of cells, and can also improve the success rate and efficiency of cell culture.

The invention relates to a preparation method and application of M1 type macrophages, and the method comprises the following steps: adding activated mouse lymphocytes into a mouse macrophage culture system for co-culture to obtain the M1 type macrophages. The invention explores the identification of CD3 / CD28 activated lymphocytes, the influence of the activated lymphocytes on the morphology of macrophages and the influence of the activated lymphocytes on macrophage subtypes, and provides a brand-new method for obtaining the mouse M1 type macrophages. According to the invention, the problem that the polarization of the macrophage cannot be completely induced by singly using the recombinant IFN-gamma to stimulate the macrophage is solved. The invention provides a novel in-vitro mouse M1 type macrophage model for experimental research, M1 type macrophages induced by activated lymphocytes can better simulate an in-vivo immunoreaction process, a required cell state is obtained by utilizing co-culture of two cells in vivo, and compared with dependence on exogenous addition of viruses or compounds, the in-vitro mouse M1 type macrophage model provided by the invention has the advantages that the in-vitro immunoreaction process can be better simulated by the M1 type macrophages induced by the activated lymphocytes; according to the invention, many potential risks and bad places can be effectively avoided.

The invention provides a method for preparing fluopyrabamide. The method is applied to fluopyrabamide production equipment. The production equipment comprises: a continuous condensation reaction device for providing a product produced by a continuous condensation reaction, and a continuous batching device and a first raw material supply device respectively connected with the continuous condensation reaction device. The continuous batching device is used to transport a 3', 4', 5'-trifluoro-2-aminobiphenyl solution into the continuous condensation reaction device. The first raw material supply device is used to continuously transport pyridine and 3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carbonyl chloride into the continuous condensation reaction device. A continuous condensation reaction occurs in the continuous condensation reaction device to generate a crude product. The production equipment also comprises a continuous post-processing device for continuously post-processing the crude product to obtain a reaction product. The problems of low reaction efficiency and low product quality caused by batch reactions in the prior art are solved.

The present invention provides a memory device and a manufacturing method thereof, wherein the memory device comprises: a substrate, a stacking structure, a channel layer and a charge storage structure. The stacking structure is located on the substrate, and comprises a plurality of insulating layers and a plurality of conductive layers stacked alternately, and the stacking structure has a hole. The channel layer is located in the hole, and comprises a first part and a second part. The number of grain boundaries of the second part is lower than the number of grain boundaries of the first part. The charge storage structure is located between the first part and the plurality of conductive layers, and the charge storage structure and the second part sandwich the first part therebetween.

This invention provides a method, apparatus, and system for correcting laser speckle contrast images, relating to the field of image processing technology. The method includes: acquiring multiple speckle images; determining a light intensity distribution map based on each speckle image; determining an original speckle contrast image of the target speckle image; determining a local light intensity variation coefficient map based on the light intensity distribution map, wherein each pixel in the local light intensity variation coefficient map represents the variation coefficient of pixel intensity in the neighborhood of the pixel in the light intensity distribution map; correcting the values ​​of corresponding pixels in the original speckle contrast image based on the values ​​of each pixel in the local light intensity variation coefficient map, obtaining and outputting a corrected speckle contrast image. Using this application can reduce the influence of uneven illumination on laser speckle images, resulting in more uniform brightness and accurate contrast in blood flow images, thereby improving the accuracy of blood flow velocity estimation.

Provided are a magnetostrictive member having a high magnetostriction constant and a high parallel magnetostriction amount and small variations in the magnetostriction constant and the parallel magnetostriction amount among members and a method for manufacturing such a magnetostrictive member. The magnetostrictive member is formed of a single crystal of an iron-based alloy having magnetostrictive characteristics, is a plate-like body having a long-side direction and a short-side direction, and has a lattice constant of a <100> orientation in the long-side direction not larger than a lattice constant average calculated from lattice constants of <100> orientations in three directions, or the long-side direction, the short-side direction, and a direction orthogonal to the long-side direction and the short-side direction.

The invention provides a blueberry seedling method and application thereof. The method comprises the following steps: selection and treatment of plants and explants, induction culture of axillary buds in an improved culture medium, zeatin (ZT) decreasing subculture, and further cultivation of subculture seedlings into commercial seedlings. According to the method provided by the invention, the basic culture medium prepared from the improved MS and WPM culture media is adopted, so that explants germinate quickly, the subculture period is short, seedlings grow well, branching is reasonable, and buds are thick and strong. The induction budding rate of the blueberries is increased to 92.5%, and the multiplication rate multiple after five times of subculture reaches 404.8 times. When commercial seedlings are cultured for 25 days by adopting a one-step seedling method, the rooting rate reaches 98.2%, the seedling culture time is averagely shortened by 19.4 days, and root systems are dispersed and uniform. According to the scheme, the seedling raising efficiency, the seedling quality and the plant growth vigor after planting are effectively improved, and the variation risk is reduced. Various root system problems which seriously affect survival, growth and yield after planting are fundamentally solved.

The present invention provides an optical lens, comprising: a first lens component, which includes a first lens barrel and a first lens installed in the first lens barrel; a second lens component, which includes a second lens barrel and a second lens installed in the second lens barrel, the second lens and the first lens together constitute an imageable optical system, and the material of the first lens barrel is different from that of the second lens barrel; and a first adhesive material, which is located in a first gap between the first lens component and the second lens component, and is suitable for supporting and fixing the first and second lens components after curing, wherein the axis of the first lens component and the axis of the second lens component have a non-zero angle. The present invention also provides a corresponding camera module and an assembly method. The present invention can reduce the difference between the state of the optical system after the first adhesive material is cured and the state of the optical system determined by active calibration by reducing the variation of the first lens component, thereby ensuring the imaging quality.

The invention relates to the technical field of adhesive tapes, in particular to an anti-static adhesive tape for electronic materials and a preparation method of the anti-static adhesive tape. The prepared adhesive tape comprises a release film layer, an adhesive layer, an anti-static coating and a base material film layer from top to bottom. The anti-static coating is located between the base material film layer and the adhesive layer, and the base material film layer is bonded with the release film through the adhesive layer. Aiming at the special requirements of low adhesion and high anti-static performance of an electronic component on the protective film in the use process, accurate regulation and control of stripping force and surface resistance are realized by optimizing the ratio of a cross-linking agent to an anti-static agent in the adhesive layer; meanwhile, the problems that a traditional anti-static adhesive tape is too high in stripping force and sensitive elements are prone to being damaged are effectively solved, and the strict requirement of electronic materials for electrostatic protection is met.

The present invention relates to the field of bioinformatics data processing technology, and in particular to a gene detection data cleaning method based on artificial intelligence. The method comprises the following steps: obtaining an original gene detection data set; performing detection quality feature recognition on the original gene detection data set to obtain detection quality feature data; performing quality screening on the detection quality feature data to obtain detection quality screening data; performing denoising on the original gene detection data set according to the detection quality screening data to obtain a preliminary gene detection data set; performing high-throughput sequencing analysis on the preliminary gene detection data set to generate high-throughput sequencing data. Therefore, the present invention uses data analysis technology, artificial intelligence technology and adaptive correction technology to achieve sequencing error correction, adaptive batch effect correction and intelligent elimination of redundant data for gene detection data, thereby making the cleaning effect of gene detection data better.

The invention relates to the technical field of skin care products, and particularly discloses an efficient acne removal composition and a preparation method thereof. The high-efficiency acne-removing composition comprises the following components in parts by mass: 0.01-0.2 part of lactoferrin; 0.01 to 0.2 part of antibacterial peptide; 0.01 to 0.1 part of lysozyme; 0.01 to 0.2 part of metallothionein; and 2.1-9 parts of a composite plant extract. The acne removing cream has the advantage of being capable of efficiently and durably removing acnes.

The application relates to an oral squamous cell carcinoma auxiliary chemical immunotherapy response method and device, which comprises the following steps: acquiring image data and baseline clinical factors of a target user, the image data comprising metabolic parameters and image features of a tumor region; performing clinical parameter standardization processing on the metabolic parameters to obtain a standardized metabolic parameter set related to treatment response; performing stability screening on the image features by using an intragroup correlation coefficient to obtain key features related to treatment response; performing regression analysis on the baseline clinical factors to identify independent prognostic factors related to treatment response in the baseline clinical factors; and performing feature fusion on the standardized metabolic parameter set, the key features and the independent prognostic factors to obtain a response rate and a prognosis prediction result of the target user. The method can more accurately and comprehensively predict the response rate and the prognosis of the target user to the auxiliary chemical immunotherapy.

The invention relates to a wheat stripe rust resistance gene Yrym112, an InDel molecular marker and an application of the wheat stripe rust resistance gene Yrym112 and the InDel molecular marker. The CDS nucleotide sequence of the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 2. The developed functional molecular marker Yrym112-InDel comprises an upstream primer sequence as shown in SEQ ID NO. 3 and a downstream primer sequence as shown in SEQ ID NO. 4. A material containing the Yrym112 gene can be divided into a disease-resistant genotype and a susceptible genotype according to the size of an amplified band. The molecular marker is used for identifying or breeding wheat stripe rust resistant germplasm resources, or wheat gene Yr5 typing and wheat molecular marker assisted breeding are used, and the breeding process of wheat disease resistant varieties is accelerated.

The corrosion-resistant radar cleaning electromagnetic valve comprises coil assemblies, a fixed assembly, a movable diaphragm assembly and an electromagnetic valve seat, the fixed assembly comprises an iron frame and a plurality of fixed iron cores, the iron frame is installed at the upper end of the electromagnetic valve seat, and the coil assemblies are arranged at the upper end of the electromagnetic valve seat and located in the iron frame side by side. A movable diaphragm group is mounted in each coil group, each movable diaphragm group comprises a movable iron core and a sealing diaphragm, the sealing diaphragm is mounted at the lower end of each movable iron core, a fixed iron core is mounted at the position, above each movable iron core, of the top of the iron frame, and a spring is mounted between each fixed iron core and the corresponding movable iron core. According to the diaphragm type electromagnetic valve, the diaphragm type design is adopted, corrosion and damage to internal parts of the electromagnetic valve caused by potential difference generated by chemical reaction between a cleaning medium and a movable iron core and the fixed iron core are eliminated, and action failure of the electromagnet is caused; and chemical reactions of active additives and the like of the medium on rubber and plastic are reduced.

The invention discloses a Microcin C7 high-efficiency expression strain and a preparation method thereof. A pCas9-sgRNA integrated vector is used for replacing an RBS region of the strain, so that the binding efficiency of an expression gene mCCA of Microcin C7 and ribosome is enhanced, translation starting variation is reduced, the translation rate is increased, and stable and high-efficiency expression of Microcin C7 can be realized. According to the fermentation culture medium provided by the invention, the corn steep liquor powder is used for replacing part of peptone, the strain fermentation efficiency is high, and the production cost is greatly reduced. The invention also provides a preparation method of the Microcin C7, the heptapeptide with a wider antibacterial spectrum is obtained, the industrial production level of the heptapeptide is improved, the application prospect of the heptapeptide in the fields of feed additives, veterinary drugs, foods and medicines is widened, and an efficient and economic biological antibacterial agent and preservative are provided for the industry.

The invention relates to the technical field of cell culture, in particular to a culture method for improving the durability of a CAR-T cell population, the culture method is characterized in that CAR-T cells are cultured by a CAR-T cell culture medium, and the CAR-T cell culture medium takes an LONZA X-VIVO15 serum-free culture medium as a basic component, the liquid medicine also comprises the following components: 2 g / L of cell factor microcapsules, 4 g / L of a fermentation compound, 20 mg / L of dextran sulfate, 2 mg / mL of vitamin C, 15 mM of 4-hydroxyethylpiperazine ethanesulfonic acid and 1.75 mM of amino acid. According to the CAR-T cell culture medium, all the components synergistically play a role and jointly provide a CAR-T cell nutrition matrix, and a tumor killing cell population with higher cell proliferation capacity and higher killing activity is obtained, so that the cultured CAR-T cells are high in proliferation rate and high in tumor killing activity, the curative effect is improved, and the durability is effectively improved.