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C-reactive protein quantitative determination kit and preparation method thereof

The invention discloses a C-reactive protein quantitative determination kit, belonging to the field of biological immune in-vitro diagnosis of medical devices. The kit comprises a calibrator, a quality control product, an antibody reagent, a magnetic particle reagent and a luminescent substrate. The invention also discloses a preparation method of the kit and a method for detecting C-reactive protein by using the kit. According to the preparation method disclosed by the invention, the antibody reagent is prepared from a C-reactive protein-coated antibody labeled by fluorescein isothiocyanate and a C-reactive protein-labeled antibody labeled by alkaline phosphatase, and the magnetic particle reagent is prepared by coupling an anti-fluorescein-isothiocyanate antibody with carboxyl magnetic beads so as to ensure that uniform mixing and separation are easily carried out in immunoreaction, and the reaction speed can be greatly increased; and a novel chemical luminescent substrate ALPS is used as the substrate, so that the sensitivity and specific performances of the kit can be improved. The detection kit disclosed by the invention is reliable in performance, high in sensitivity and wide in linear range, and can be matched with semi-automatic and full-automatic instruments for use.
Owner:NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV +1
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