The invention relates to the field of molecular biology, genetic engineering technology and biological medicine, in particular to a small interfering RNA (ribonucleic acid), a short hairpin RNA and a recombined carrier for SOX4 gene targets, and application of the recombined carrier. The small interfering RNA comprises a positive-sense strand shown as SEQ ID NO.1 and an antisense strand shown as SEQ ID NO.2. The short hairpin RNA comprises a positive-sense strand shown as SEQ ID NO.3 and an antisense strand shown as SEQ ID NO.4. The recombined carrier comprising the short hairpin RNA can be transcribed in a host cell to obtain SOX4-shRNA, the small interfering RNA can be generated after the SOX4-shRNA is processed by the host cell, a messenger RNA of SOX4 is targeted, expression of the SOX4 is reduced, the gene silencing effect is caused, multiplication of esophageal squamous carcinoma cells is inhibited effectively, and aging of the esophageal squamous carcinoma cells induced by chemotherapeutic adriamycin is promoted, so that the recombined carrier has application value in tumor resistance, and the foundation is laid for further research on the function of the SOX4 in aging and escaping of tumor cells and for research and development of targeted SOX4 drugs.