The invention discloses a kit for rapid and simultaneous detection of MTHFR and
MTRR gene polymorphisms and a method thereof, and belongs to the technical field of
gene detection. According to the kitfor rapid and simultaneous detection of MTHFR and
MTRR gene polymorphisms, the gene polymorphisms include the following types of gene polymorphisms: MTHFR 677C being greater than T, MTHFR 1298A beinggreater than C and
MTRR 66A being greater than G. The kit includes a sample treatment
reagent used for rapid treatment of human
whole blood samples, gene detection reagents which are premixed and single-packaged, a
positive control and a
negative control. The gene detection reagents comprise MTHFR and MTRR wild-type and
mutant-type gene detection reagents. On the basis of guaranteeing the detection specificity and sensitivity, convenient, fast and efficient detection is realized, detection operating steps are reduced, detection reaction time is shortened, and the production cost and the detection cost are also reduced. The invention is beneificla to clinical detection, analysis and application.