The invention discloses a kit of MTRR gene polymorphism rapid test. The kit comprises PCR reaction liquid; the PCR reaction liquid is prepared from 0.04-0.09 U / microliter of DNA polymerase, 0.1-0.5 mM of dNTPS, 0.5-1.5 X of 5X PCR buffer solution, 1-2.5 mM of MgCl2, 0.0005-0.015%(w / v) of lauryl sodium sulfate, 0.001-0.03%(w / v) of polyethylene glycol octylphenol ether, 0.2-1.0 muM of upstream primer, 0.2-1.0 muM of downstream primer, 0.3-0.5 muM of mutant molecular beacon and 0.3-0.7 muM of wild molecular beacon, and is used for testing cell samples. The technical problem to be solved is to provide a primer, molecular beacon and kit of MTRR gene polymorphism rapid test and a testing method thereof through which sampling can be conducted in the painless environment and which are simple in operation, low in cost, rapid in test, short in time and high in success rate, and accordingly guidance is provided for individualization folic acid use, and the fetal anomaly risk is reduced.