Primers, probes and kit for detecting genes related to metabolic capacity of folic acid

Abstract

The invention discloses primers and probes for detecting genes related to metabolic capacity of folic acid and belongs to the technical field of biomedicine and also provides a kit containing the primers and probes. The primers and probes for detecting the genes related to metabolic capacity of folic acid have good specificity and can improve detection specificity of SNP sites; when the kit is used for detecting polymorphism of MTHFR and MTRR genes, the accuracy of detection result and sequencing results is up to 100%, homozygous wild type, homozygous mutant type and heterozygosis type of three sites of a sample can be detected by three tubes simultaneously, and the detection efficiency is high; meanwhile, compared with judged results in delta Ct value method, the judged results are easierto judge, can be accurately typed by typing software and affirmed and rechecked through fluorescence quantitative PCR curves and are more accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a primer, a probe and a kit for detecting genes related to folic acid metabolism capacity. Background technique [0002] Folic acid is a water-soluble B vitamin that cannot be synthesized by the human body and must be ingested from the outside world. The demand for folic acid by pregnant women is 4 times higher than that of normal people. Appropriate supplementation of folic acid can effectively reduce the probability of fetal malformation; supplementation of folic acid for middle-aged and elderly people can obviously prevent and treat many diseases. Studies have found that appropriate supplementation of folic acid can effectively reduce the risk of fetal malformation. The risk of stroke can prevent and delay the occurrence of cognitive impairment in the elderly. [0003] The ability to metabolize folic acid is affected by genetic factors, and mutations in certai...

AI Technical Summary

Problems solved by technology

[0005] The Chinese patents whose publication numbers are CN107254520A and CN108004309A use DNA direct sequencing method to detect MTHFR gene and MTRR gene after PCR amplification, but this method needs to purify the amplified sample, re-amplify and analyze the sequence, and the detection steps are cumbersome , complex, high requirements on the environment and operators, expensive instruments and equipment, high cost, and certain limitations in clinical practice; Chinese patents with publication numbers CN104774944A and CN105986010A disclose the use of mass spectrometry to detect genotypes related to folic acid metabolism Method and kit, this method is distinguished by the length of the primer and the relative molecular mass of the base, with high throughput and high sensitivity, but it needs to be combined with a mass spectrometer for typing, the equipment used is expensive, and the operator has high technical requirements. Limited availability and popularity

At the same time, the existing kits for nucleic acid extraction and detection of folic acid metabolism from whole blood samples have problems such as long operation time and high reagent costs.

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