98 results about "Metabolic power" patented technology

Metabolic energy capacity enhancing compositions and methods for reducing oxidative stress and improving vitality in a mammal are disclosed. A composition for increasing metabolic energy capacity may be in a palatable liquid formulation or a solid dosage form and typically includes an anti-oxidant containing phytonectar and an energy catalyst. An anti-oxidant may include a polyphenol, anthrocyanin, bioflavonoid, proanthocyanidin, and a xanthone. An energy catalyst may include a mineral, vitamin, co-vitamin, carbohydrate and a lipid. In a presently preferred embodiment a composition includes phytonectar extracts from grape, aloe vera, apple, morinda citrifolia, scullcap, blueberry, prune, cranberry, elderberry, bilberry, and gentain and a mineral blend containing calcium, magnesium, manganese, zinc, chromium, selenium, iron, copper, molybdenum, vanadium, potassium, iodine, and cobalt. A method for increasing metabolic energy capacity in a mammal may include consuming a chemical component having the ability to undergo oxidation, producing free radicals and administering a composition having an anti-oxidant containing phytonectar and an energy catalyst.

The invention provides a non-naturally occurring set of microbial organisms. The set of organisms includes: at least a first constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize a first carbon substrate and having substantially impaired metabolic capacity for a second carbon substrate, and at least a second constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize the second carbon substrate and having substantially impaired metabolic capacity for the first carbon substrate, wherein a co-culture of the at least first and second CMOs exhibit simultaneous metabolism of a mixture having the first and second carbon substrates compared to either CMO alone. Simultaneous metabolism of a mixture having first and second carbon substrates can include an enhanced rate of metabolism of the first and second substrates compared to either CMO alone. Also provided is a bioprocess for producing a chemical compound. The bioprocess includes co-culturing a non-naturally occurring set of microbial organisms in a mixture having at least a first and a second carbon substrate under conditions sufficient for biosynthesis of a target chemical compound, the set of non-naturally occurring microbial organisms including: at least a first constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize the first carbon substrate and having substantially impaired metabolic capacity for the second carbon substrate, and at least a second constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize the second carbon substrate and having substantially impaired metabolic capacity for the first carbon substrate, wherein a co-culture of the at least first and second CMOs exhibit simultaneous metabolism of a mixture having the first and second carbon substrates compared to either CMO alone. Simultaneous metabolism of a mixture having first and second carbon substrates can include an enhanced rate of metabolism of the first and second substrates compared to either CMO alone.

This invention is to use knowledge pattern learning and search system for selecting microorganisms to produce useful materials and to generate clean energy from wastes, wastewaters, biomass or from other inexpensive sources. The method starts with an in silico screening platform which involves multiple steps. First, the organisms' profiles are compiled by linking the massive genetic and chemical fingerprints in the metabolic and energy-generating biological pathways (e.g. codon usages, gene distributions in function categories, etc.) to the organisms' biological behaviors. Second, a machine learning and pattern recognition system is used to group the organism population into characteristic groups based on the profiles. Lastly, one or a group of microorganisms are selected based on profile match scores calculated from a defined metabolic efficiency measure, which, in term, is a prediction of a desired capability in real life based on an organism's profile. In the example of recovering clean energy from treating wastewaters from food process industries, domestic or municipal wastes, animal or meat-packing wastes, microorganisms' metabolic capabilities to digest organic matter and generate clean energy are assessed using the invention, and the most effective organisms in terms of waste reduction and energy generation are selected based on the content of a biowaste input and a desired clean energy output. By selecting a microorganism or consortia of multiply microorganisms using this method, one can clean the water and also directly generate electricity from Microbial Fuel Cells (MFC), or hydrogen, methane or other biogases from microorganism fermentation. In addition, using similar screening method, clean hydrogen can be recovered first from an anaerobic fermentation process accompanying the wastewater treatment, and the end products from the fermentation process can be fed into a Microbial Fuel Cell (MFC) process to generate clean electricity and at the same time treat the wastewater. The invention can be used to first select the hydrogenic microorganisms to efficiently generate hydrogen and to select electrogenic organisms to convert the wastes into electricity. This method can be used for converting wastes to one or more forms of renewable energies.

The invention is a near-infrared nondestructive tissue detecting method for skeletal muscle supersession function's blood transporting parameters, its character: in view of different tested objects (sportsman and non sportsman) and muscle tissue's oxyhemoglobin variation (deta HbO2) as well as reduced hemoglobin variation (deta Hb), it selects proper detecting distance and gives the detecting steps, algorithm and system design which can judge parameters of oxygen supersession ability of muscle tissue. The sensor can be composed of three LEDs and a photoelectric receiver. The whole testing system contains sensor, preamplifying circuit, A/D converter, inserted microprocessor, power supply, liquid crystal and touch screen. It has the advantages of high detecting sensitivity and simple detecting system structure.

The invention discloses a primer system for detecting folic acidinheritancemetabolic capability and calcium absorptioninheritance to detect related genepolymorphic site (SNP). A product prepared on the basis of the primer system is capable of detecting folic acidinheritancemetabolic capability and calcium absorption related genepolymorphic sites simultaneously. By using the product, folic acidinheritancemetabolic capability related genepolymorphic sites are detected, reference bases are provided to instruct women preparing for pregnancy and pregnantwomen to take folic acid, and meanwhile detection on calcium absorption related genepolymorphic sites can also instruct the pregnant women to scientifically and reasonably supplement calcium nutrients in the pregnancy period. By adopting the primer system, 5 genepolymorphic sites on different genes can be simultaneously detected within one reaction system, the cost is relatively low when being compared with that of techniques such as sequencing and real-time fluorescence quantification PCR (Polymerase Chain Reaction), the operation is relatively simple, and the accuracy and the sensitivity are improved.

The present invention provides a culture method for promoting autotrophy microalgae neutral lipid accumulation, wherein microalgae having neutral lipid accumulation metabolism capability at a proliferation later stage is subjected to neutral lipid accumulation culture under a 400-550 nm light wave condition. According to the method, under the premise of proliferation culture of the obtained first step cells to achieve the maximum biomass, microalgae neutral lipid metabolism rapidly responses in a short time through temperature reduction on the microalgae liquid and a synergism effect of the illumination condition so as to achieve a purpose of promotion and increase of the neutral lipid accumulation efficiency and the neutral lipid accumulation amount. The blue filter membrane is a plastic film, and can be subjected to large-scale application and modification. The nitrogen limitation stress condition can be achieved through N/P ratio inversion regulation without a microalgae and media separation process. In addition, the process in the large-scale production can be simplified.

The object of the present invention is to provide a method of preparing excellent cultured muscle cells having high metabolic capacity and insulin responsiveness, and further provide a method for the measurement of sensitive metabolic capacity using the cells. Moreover, its purpose is to provide a culture system/culture apparatus that can smoothly translocate such highly advanced cultured muscle cells intact to activity evaluation systems of a number of drugs. Moreover, the object of the present invention is to provide cultured muscle cells that are very suitable for measurement of the membrane-translocation activity of GLUT4 in an extraneous stimulus-dependent manner such as insulin, etc., and to provide a method for the measurement of the membrane-translocation activity of GLUT4 using the cells. The present invention is a method of preparing myotube cells, comprising a step (1) of culturing myoblast cells, a step (2) of differentiation-inducing the myotube cells into the myoblast cells in a culture medium with a high content of amino acids, and a step (3) of applying an electric pulse to the differentiation-induced myotube cells, and a method for the measurement of insulin-dependent sugar uptake using the myotube cells prepared by said method, and relates to the method for the measurement, comprising applying insulin stimulation by culturing the cells in a culture medium containing insulin, culturing the cells in the culture medium further supplemented with sugar, and measuring the sugar uptake. Furthermore, the present invention relates to a differentiation-type culture myotube cell constitutively expressing a recombinant GLUT4 having a labeled substance at its extra-cellular site, which is prepared by co-culturing wild-type myoblast cells and recombinant myoblast cells constitutively expressing said recombinant GLUT4, and a method for the measurement of membrane-translocation activity of the recombinant GLUT4 using the cells, and particularly a method for the measurement of insulin-dependent sugar uptake activity.

The invention discloses an anti-caries polypeptide capable of preventing bacteria and promoting remineralization, a polypeptide derivative or a polypeptide pharmaceutical salt thereof. The anti-caries polypeptide contains an amino acid sequence a and an amino acid sequence b; the amino acid sequence a is Xn, wherein X is used for representing S, T, Y, K, R, N, or Q, n is used for representing the amino acid repeat times, and is an integer selected from 5 to 8; the amino acid sequence b is of a alpha double helix structure, is positively charged, and contains 8 to 12 amino acids, and the hydrophilic amino acids and hydrophobic amino acid in the amino acid sequence b appear alternately. The functional anti-caries polypeptide capable of preventing cariogenic bacteria and promoting remineralization is capable of inhibiting growth of streptococcus mutans, inhibiting formation of biological membrane of streptococcus mutans, reducing the metabolism capability of formed biological membrane of streptococcus mutans, killing viable bacteria in the formed biological membrane of streptococcus mutans, promoting remineralization of demineralization tooth enamel, and reducing caries depth and mineral loss of demineralization tooth enamel, and possesses excellent stability, and no obvious cytotoxicity.

The invention provides a primer set, application, a product and a method for detecting SNP sites related to drug metabolism in children, and relates to the field of biotechnology. The primer set of the SNP sites related to drug metabolism in children provided by the invention can be used to detect the metabolism capacity of thermoanalgesics, metabolism capacity of respiratory system drugs, metabolism capacity of neurological and psychiatric drugs, metabolism capacity of cardiovascular and cerebrovascular drugs, metabolism capacity of anti-infective drugs, metabolism capacity of endocrine drugs, metabolism capacity of digestion system drugs, metabolism capacity of anesthetic drugs and metabolism capacity of chemotherapy and immunosuppressant. Specific detection of site mutation related to common drug metabolism in children can be realized by the primer set, the accuracy is high, the detection cycle can be tremendously shortened, and the detection cost is reduced at the same time. Moreover, the needs of a drug metabolism can be predicted by the detection results, the metabolism capacity of a variety of drugs can also be comprehensively evaluated, and scientific reference is providedfor the individualized medication of children.

A Baipichi strain CZ-81 (CGMCC No.1016) is disclosed, which features high stability, metablitic power and safety. Its artificial culture method includes slant culture, shake culture, seeding-tank culture, fermenting and extracting. Its advantages are short time dicreased by 20%, and high content of active components.

The invention relates to folic acid heredity metabolism ability detection using mass spectrum, and discloses a primer system for detecting folic acid heredity metabolism ability-associated gene polymorphism sites (SNP). A product produced on the basis of the primer system can realize simultaneous detection of the folic acid heredity metabolism ability-associated gene polymorphism sites. The product is used to detect the folic acid heredity metabolism ability-associated gene polymorphism sites in order to provide reference basis for guidance of reasonable administration of women preparing for pregnancy and pregnant women. The system and the product allow three gene polymorphism sites on different genes to be simultaneously detected in a reaction system; and compared with sequencing, real-time fluorescent quantitative PCR and other technologies, a method adopting the system or the product has the advantages of low cost, simple operation, and increased accuracy and sensitivity.

The invention relates to a skin external preparation, in particular to an external preparation capable of promoting the active circulation of cells. The external preparation comprises the following components in percentage by mass: 20 to 50 percent of glycosaminoglycan, 20 to 50 percent of amino acid, 10 to 30 percent of natural extract, 5 to 10 percent of diguanosine tetraphosphate (Gp4g) and 1 to 5 percent of inorganic salt; and micro elements are added in an amount which is 0.001 to 0.05 percent based on the total mass of the previous components are added. The external preparation is prepared by using distilled water. The external preparation capable of promoting the active circulation of cells, which is provided by the invention, can be externally sprayed or applied on skin to quicklysupply inorganic salt to skin, activate cells and accelerate the metabolism of skin cells; and the external preparation also can supply energy and required water to cells, improve the micro circulation of skin and effectively clean free radicals, activate the self-healing system of human body, quickly penetrate and freely enter and leave cells, repair cells safely, naturally and gently, accelerate cell division, increase metabolic capacity and delay aging.

The invention provides a gene noninvasive detection kit for preventing neural tube defects of newborns. The kit comprises specific primers, DNA sequencing primers, a PCR (Polymerase Chain Reaction) reaction assembly, a PCR product purification assembly, a DNA sequencing reaction assembly and the like, wherein the specific primers are used for detecting No. rs1801133 SNP locus (MTHFR C677T) and No. rs1801131 SNP locus (MTHFR A1298C) on 5, 10-methylene tetrahydrofolate reductase (MTHFR) and No. rs1801394 SNP locus (MTRR A66G) on 5, 10-methylene methyl transferase reductase (MTRR). The kit evaluates the risk level of pregnant women giving birth to infants with neural tube defects by detecting the genotype of a single nucleotide polymorphism locus closely related to the main genetic factor causing newborn defects, namely female folic acid metabolism ability, and the pregnant women are individually instructed to supplement folic acid according to the gene detection result of each client. The method provided by the invention accords with the situation of our country, oral mucosa cell sampling is adopted as a sampling method which is painless and noninvasive, and cross infection is avoided. The sequencing detection results are accurate and reliable, expensive imported special instruments are not needed, and the method is easy to popularize and spread.

The invention relates to a multi-spectral physical therapy intelligent control method and device, and belongs to the technical field of medical instruments. According to the method, light-emitting light sources are used for emitting light of differential types of spectrum including far infrared light, red light, yellow light, blue light and the like, on the basis of the chemical action of short-wave light on cells and the heat effect of long-wave light, matching of light of differential types of spectrum in different proportions according to different levels of light energy is achieved through a spectral energy matching algorithm, light of different types of spectrum acts on subcutaneous tissue of a human body in a mutually cooperative mode so that tissue cells can be activated, the metabolism ability of cells is improved, and the purposes of preventing and curing disease are achieved. According to the device and method, recombination spectrum phototherapy is achieved, and the defects that when a method with a single light source in use is adopted, for example, only far infrared is in use, treatment is incomplete, and efficiency is low can be avoided; physiotherapy can be carried out on the human body intelligently and efficiently in a multi-directional mode, and the device and method are suitable for being widely applied and popularized.

The invention discloses a technology for increasing the nutrient value of a cottonseed meal feed by a microorganism fermentation method. A modern bacterium mixing microorganism fermentation technique,an enzyme technique and the like are integrated, and a cottonseed meal protein feed which is high in protein, high in amino acid content, low in free gossypol content and high in feed return rate isresearched and developed. The specific technology comprises two working procedures of performing bacterium mixing aerobic fermentation and bacterium mixing anaerobic fermentation, wherein aerobic fermentation bacterium groups comprise aspergillus niger, microzymes, bacillus subtilis and bacillus psychrosaccharolyticus; and anaerobic fermentation bacterium groups comprise clostridium butyricum andlactic acid bacteria. According to the technology, multiple bacterium mixing fermentation is adopted, and through combination of different metabolic capabilities, the complex metabolism which is difficult to complete by single strains is completed; through the synergic fermentation among microorganisms, the important positive effect on effective transformation of various raw materials and increaseof the quality of the protein feed is achieved; and the technology has the advantages of being good in product efficacy, simple and efficient in technology, short in fermentation period, low in production cost and the like, and is easy in industrialized large-scale production.

The present invention relates, generally to a method of determining and assessing cytochrome P450 2C19-related (CYP2Cl9) metabolic capacity in an individual mammalian subject via a breath assay, by determining the relative amount of ¹³CO₂ exhaled by the subject upon intravenous or oral administration of a ¹³C-labeled CYP2C19 substrate compound. The present invention is useful as a non-invasive, in vivo assay for evaluating CYP2C19 enzyme activity in a subject using the metabolite ¹³CO₂ in expired breath, to phenotype individual subjects and to determine the selection, optimal dosage and timing of drug administration.

The present invention relates to a novel customized sRNA that reduces gene expression in prokaryotic cells, a preparation method thereof, and the use thereof, and more particularly to a synthetic sRNA comprising an Hfq binding site, derived from the sRNA of any one of MicC, SgrS and MicF, and a region that base-pairs with the target gene mRNA, and to a preparation method thereof and the use thereof. The synthetic sRNA according to the invention has an advantage in that the degree of inhibition of the target gene can be controlled by regulating the ability of the synthetic sRNA to bind to the mRNA of the target gene. The use of the synthetic sRNA that regulates the expression of the target gene makes it possible to effectively construct a recombinant microorganism without using a conventional gene deletion method and to reduce the expression of the target gene, and thus the synthetic sRNA is useful for the production of recombinant microorganisms. Also, the synthetic sRNA can be quickly applied to various strains, and thus is very suitable for the measurement of metabolic capabilities of strains and the selection of the most suitable strain. In addition, recombinant microorganisms, which are obtained by metabolic flux manipulation using the synthetic sRNA and produce tyrosine or cadaverine with high efficiency, are useful in the drug and industrial fields. In other words, the use of the sRNA according to the present invention can make it easy to select target genes whose expression is to be inhibited for the highly efficient production of metabolites. Accordingly, the synthetic sRNA can be used to construct recombinant strains for efficient production of various metabolites and to establish efficient methods for production of various metabolites, and thus is highly useful.

The invention provides a method for improving the yield of a vitamin PQQ according to a fermentation method. The method provided by the invention comprises the following steps: firstly, breeding hyphomicrobium with good growing state, vigorous vitality and strong metabolic capability, then allocating a culture medium and performing strain activation on the selected hyphomicrobium by the solid culture medium, in which trace element solution is added, for getting ready for selection of optimal strains; inoculating the hyphomicrobium on a specially-made shake flask seed culture medium for cultivation under appropriate conditions to enlarge the quantity of floras of the excellent hyphomicrobium, so as to obtain shake flask seed solution; then inoculating the shake flask seed solution on a specially-made seed culture medium for cultivation under appropriate conditions to the logarithmic phase, so as to obtain seed bacteria solution; finally, inoculating the seed bacteria solution on a specially-made fermentation culture medium. The fermentation culture medium provided by the invention can not only provide sufficient nutrients for the hyphomicrobium, but also inhibit undesirable growth factors, promote the hyphomicrobium to quicken the metabolism velocity and improve the yield of the vitamin PQQ; fermentation solution containing the vitamin PQQ is obtained; the fermentation yield of the hyphomicrobium is 1 g/L or higher, and the extraction rate of the hyphomicrobium is 50% or higher.

The invention relates to functional clean daily ration for improving health of pregnant sows and improving reproductive performance of the pregnant sows. The clean daily ration contains wood fiber made of a mixture of manioc waste, beet pulp and apple pomace, and the additive amount of the wood fiber and the mass ratio of the manioc waste to the beet pulp to the apple pomace in the wood fiber are controlled. According to the clean daily ration, the content of mycotoxin in compound feed is reduced remarkably, the crude fiber level and structure of the daily ration are improved, and the daily ration has an exact effect on improving constipation of the pregnant sows; a traditional Chinese medicine component is introduced into the clean daily ration, the livers of the pregnant sows are protected, active detoxifying and metabolic capability to mycotoxin of the pregnant sows is improved, the pH value of faeces and the number of escherichia coli are reduced, the number of lactobacilli is increased, and the immunologic function of the overall system of the pregnant sows is improved fundamentally; the clean daily ration improves the reproductive performance of the pregnant sows, increases live piglet delivery number, and reduces the number of weak piglets and stillborn foetuses.

The invention relates to the field of the waste gas purification, and specifically relates to a growth promoting flora for improving waste gas treatment efficiency and a waste gas treatment method. The growth promoting flora for improving waste gas treatment efficiency provided by the invention comprises bifidobacterium, lactic acid bacteria, actinomycetes and bacillus mucilaginosus in a mass ratio of 0.9-1.1:0.8-1.0:0.9-1.1:2.7-3.5. The growth promoting flora disclosed by the invention does not directly participate into the pollutant degradation, but can promote the growth and multiplying ofa target flora mixture directly participating into the pollutant degradation, so that the metabolic capacity on a pollutant by the target flora mixture can be always maintained at a high level, and the treatment efficiency on the industrial waste gas reaches up to 99.8% or more.

The invention discloses a fermentation culture medium and a method for preparing mupirocin by virtue of the fermentation culture medium. The method comprises the following steps: breeding pseudomonasfluorescence with good growth state, vigorous vitality and strong metabolic capability, blending a culture medium, carrying out strain activation on pseudomonas fluorescence, and selecting optimal strains for later use; and inoculating the strains to a special seed culture medium so as to increase the quantity of excellent pseudomonas fluorescence, carrying out culturing to a logarithmic phase soas to obtain a seed bacteria solution, and finally, inoculating the seed bacteria solution to the special fermentation culture medium. According to the fermentation culture medium, sufficient nutrients can be provided for pseudomonas fluorescence, the growth and metabolism speeds of pseudomonas fluorescence can be increased, a fermentation liquid containing mupirocin is obtained, and the content of mupirocin in the fermentation liquid is over 6000ug/mL.

The invention provides a primer group, an application, a product and a method for detecting SNP (single nucleotide polymorphism) loci related to drug metabolism capacity of rheumatic immunological diseases, and relates to the technical field of biology. The primer group for detecting the SNP loci related to the drug metabolism capacity of the rheumatic immunological diseases can be used for detecting metabolism capacity of an immunosuppressor, metabolism capacity of an immunomodulator, metabolism capacity of an anti-inflammatory agent, metabolism capacity of a TNF inhibitor, metabolism capacity of a B cell regulator, metabolism capacity of an IL-6 inhibitor, metabolism capacity of a uric acid lowering agent and metabolism capacity of a uricosuric agent. The primer group is designed for main mutation sites related to common drug metabolism of the rheumatic immunological diseases, can realize specific detection for locus mutation related to common drug metabolism of the rheumatic immunological diseases, and can greatly shorten detection cycle and reduce detection cost.

The invention discloses a method for cultivating pumpkins for diabetes patients. The method is characterized in that 0.5kg-2kg of zinc sulfate, 0.5kg-2kg of manganese sulfate, 20g-200g of chromic chloride and 20g-200g of sodium selenite are respectively weighed and evenly mixed to manufacture mixed fertilizer. The mixed fertilizer and organic fertilizer are evenly stirred by the mass ratio of 1:20-50. Before the pumpkins are planted, the fertilizer is used as base fertilizer and used at a time when soil is ploughed, and the use amount in each mu is 20kg-40kg. According to the method for cultivating the pumpkins for the diabetes patients, the multi-micronutrient fertilizer is reasonably used, the bioconversion function of the pumpkins is used, and the content of the human body necessary microelements of chromium, zinc, selenium and manganese in the pumpkins is improved, wherein the human body necessary microelements have the functions of blood sugar level regulation and pancreas islet repair. When the pumpkins with the microelements are eaten for a long time, the glycometabolism capability of the human body is effectively improved, so that the occurrence of the diabetes is prevented and controlled.