Technology for increasing nutrient value of cottonseed meal feed by microorganism fermentation method

A technology of microbial fermentation method and nutritional value, applied in the field of bioengineering, can solve the problems of weak industrialization transformation ability, complex fermentation process, long fermentation cycle, etc., and achieve the effect of improving the nutritional value of feed, reducing environmental pollution, and affecting value enhancement.

Active Publication Date: 2018-06-29
桂林精成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A comprehensive analysis of the existing research results shows that although a series of research results have been obtained in the preparation of cottonseed protein feed by microbial fermentation, there are still problems s

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A process for improving the nutritional value of cotton meal for feeding by using a microbial fermentation method, the specific process steps include:

[0033] (1) Pretreatment: select non-mildew cotton meal, crush it through a 40-mesh sieve, and adjust the moisture content of the cotton meal to 40-60%;

[0034] (2) Preparation of seed liquid:

[0035] Under this technical scheme, the yeast is a mixed strain of Candida utilis and Kluyveromyces fragilis, the dosage ratio is 7:3, and the lactic acid bacteria is Lactobacillus acidophilus;

[0036] ① The preparation method of the seed liquid of Aspergillus niger is: take fresh bran, sieve the fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0 to 1.3, and mix until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL Erlenmeyer flasks, each 2000mL Erlenmeyer flask contains about 100g of wet bran, sterilize 40-60min under 0.1MPa gauge pressure, cool to 35℃, and ins...

Embodiment 2

[0046] A process for improving the nutritional value of cotton meal for feeding by using a microbial fermentation method, the specific process steps include:

[0047] (1) Pretreatment: select non-mildew cotton meal, crush it through a 40-mesh sieve, and adjust the moisture content of the cotton meal to 40-60%;

[0048] (2) Preparation of seed liquid:

[0049] Under this technical scheme, the yeast is beer yeast, and the lactic acid bacteria is Lactobacillus bulgaricus;

[0050] ① The preparation method of the seed liquid of Aspergillus niger is: take fresh bran, sieve the fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0 to 1.3, and mix until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL Erlenmeyer flasks, each 2000mL Erlenmeyer flask contains about 100g of wet bran, sterilize 40-60min under 0.1MPa gauge pressure, cool to 35℃, and insert 1-2 into each Erlenmeyer flask. The spores of Aspergillus oryzae cv-8 ...

Embodiment 3

[0060] A process for improving the nutritional value of cotton meal for feeding by using a microbial fermentation method, the specific process steps include:

[0061] (1) Pretreatment: select non-mildew cotton meal, crush it through a 40-mesh sieve, and adjust the moisture content of the cotton meal to 40-60%;

[0062] (2) Preparation of seed liquid:

[0063] Under this technical scheme, the yeast is Saccharomyces cerevisiae, and the lactic acid bacteria is Lactobacillus plantarum;

[0064] ① The preparation method of the seed liquid of Aspergillus niger is: take fresh bran, sieve the fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0 to 1.3, and mix until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL Erlenmeyer flasks, each 2000mL Erlenmeyer flask contains about 100g of wet bran, sterilize 40-60min under 0.1MPa gauge pressure, cool to 35℃, and insert 1-2 into each Erlenmeyer flask. The spores of Aspergillus...

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Abstract

The invention discloses a technology for increasing the nutrient value of a cottonseed meal feed by a microorganism fermentation method. A modern bacterium mixing microorganism fermentation technique,an enzyme technique and the like are integrated, and a cottonseed meal protein feed which is high in protein, high in amino acid content, low in free gossypol content and high in feed return rate isresearched and developed. The specific technology comprises two working procedures of performing bacterium mixing aerobic fermentation and bacterium mixing anaerobic fermentation, wherein aerobic fermentation bacterium groups comprise aspergillus niger, microzymes, bacillus subtilis and bacillus psychrosaccharolyticus; and anaerobic fermentation bacterium groups comprise clostridium butyricum andlactic acid bacteria. According to the technology, multiple bacterium mixing fermentation is adopted, and through combination of different metabolic capabilities, the complex metabolism which is difficult to complete by single strains is completed; through the synergic fermentation among microorganisms, the important positive effect on effective transformation of various raw materials and increaseof the quality of the protein feed is achieved; and the technology has the advantages of being good in product efficacy, simple and efficient in technology, short in fermentation period, low in production cost and the like, and is easy in industrialized large-scale production.

Description

Technical field [0001] The invention relates to a process for improving the nutritional value of cotton meal by using a microbial fermentation method, in particular to a production process for preparing fermented cotton meal with high protein content and high feed rate by synergistic fermentation of multiple probiotics, belonging to the field of bioengineering . Background technique [0002] The shortage of feed resources, especially protein feed, is one of the bottlenecks restricting the development of my country's animal husbandry. How to effectively alleviate the shortage of protein feed resources in my country is a major issue to be solved. Protein raw materials are the foundation of the development of the feed industry. The shortage of protein raw materials and high prices in my country have become a bottleneck restricting the development of animal husbandry. At present, with the improvement of people’s living standards and the emphasis on quality of life, the demand for me...

Claims

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Application Information

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IPC IPC(8): A23K10/30A23K10/37A23K10/33A23K10/12A23K20/105A23K20/26A23K20/189
CPCA23K10/12A23K10/30A23K10/33A23K10/37A23K20/105A23K20/189A23K20/26Y02P60/87
Inventor 不公告发明人
Owner 桂林精成生物科技有限公司
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