Technology for increasing nutrient value of cottonseed meal feed by microorganism fermentation method
A technology of microbial fermentation method and nutritional value, applied in the field of bioengineering, can solve the problems of weak industrialization transformation ability, complex fermentation process, long fermentation cycle, etc., and achieve the effect of improving the nutritional value of feed, reducing environmental pollution, and affecting value enhancement.
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Embodiment 1
[0032] A process for improving the nutritional value of cotton meal for feeding by using a microbial fermentation method, the specific process steps include:
[0033] (1) Pretreatment: select non-mildew cotton meal, crush it through a 40-mesh sieve, and adjust the moisture content of the cotton meal to 40-60%;
[0034] (2) Preparation of seed liquid:
[0035] Under this technical scheme, the yeast is a mixed strain of Candida utilis and Kluyveromyces fragilis, the dosage ratio is 7:3, and the lactic acid bacteria is Lactobacillus acidophilus;
[0036] ① The preparation method of the seed liquid of Aspergillus niger is: take fresh bran, sieve the fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0 to 1.3, and mix until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL Erlenmeyer flasks, each 2000mL Erlenmeyer flask contains about 100g of wet bran, sterilize 40-60min under 0.1MPa gauge pressure, cool to 35℃, and ins...
Embodiment 2
[0046] A process for improving the nutritional value of cotton meal for feeding by using a microbial fermentation method, the specific process steps include:
[0047] (1) Pretreatment: select non-mildew cotton meal, crush it through a 40-mesh sieve, and adjust the moisture content of the cotton meal to 40-60%;
[0048] (2) Preparation of seed liquid:
[0049] Under this technical scheme, the yeast is beer yeast, and the lactic acid bacteria is Lactobacillus bulgaricus;
[0050] ① The preparation method of the seed liquid of Aspergillus niger is: take fresh bran, sieve the fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0 to 1.3, and mix until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL Erlenmeyer flasks, each 2000mL Erlenmeyer flask contains about 100g of wet bran, sterilize 40-60min under 0.1MPa gauge pressure, cool to 35℃, and insert 1-2 into each Erlenmeyer flask. The spores of Aspergillus oryzae cv-8 ...
Embodiment 3
[0060] A process for improving the nutritional value of cotton meal for feeding by using a microbial fermentation method, the specific process steps include:
[0061] (1) Pretreatment: select non-mildew cotton meal, crush it through a 40-mesh sieve, and adjust the moisture content of the cotton meal to 40-60%;
[0062] (2) Preparation of seed liquid:
[0063] Under this technical scheme, the yeast is Saccharomyces cerevisiae, and the lactic acid bacteria is Lactobacillus plantarum;
[0064] ① The preparation method of the seed liquid of Aspergillus niger is: take fresh bran, sieve the fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0 to 1.3, and mix until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL Erlenmeyer flasks, each 2000mL Erlenmeyer flask contains about 100g of wet bran, sterilize 40-60min under 0.1MPa gauge pressure, cool to 35℃, and insert 1-2 into each Erlenmeyer flask. The spores of Aspergillus...
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