34 results about "Bacillus psychrosaccharolyticus" patented technology

The invention provides a microbial agent for preventing and treating cumquat huanglongbing and a preparation method thereof, and particularly provides a microbial agent for preventing huanglongbing of cumquat, citrus shantianju and citrus and a preparation method thereof. The microbial agent is formed by fermenting and then mixing bacteria like Gulake streptomyces, Kawuer streptomyces, bacillus psychrosaccharolyticus, nissl bacillus and tufted trichoderma proportionally. Procreation and propagation of gram negative bacteria are inhibited by means of root irrigation and foliage spray, bacteria are used to treat bacteria, and harmful pathogenic bacteria in soil are killed, so that tree root systems are more developed, tree cell tubes are smooth, yellow leaves of fruit trees are turned green, and healthy new treetops are enabled to grow; the microbial agent generates insecticidal antibiotics like flavensomycin and inhibits or damages development process of eubactericera and eggs of other disease-transmitting insect (like citrus aphid), so that adult rate is reduced, orchard pest occurrence quantity is reduced, transmission media of huanglongbing are cut off, and effects on preventing and controlling citrus huanglongbing are realized.

The invention relates to a production method of a liquid water-soluble fertilizer containing a saccharophilia bacillus agent and amino acid. The method comprises: grinding dried seaweed into powder, adding the seaweed powder into water according to a certain ratio, putting the obtained product in a fermentation tank, and performing sterilization at a certain temperature for certain time; preparing a seed solution, which includes performing screening to obtain YIC-ALG3 saccharophilia bacillus as a fermentation strain for degrading alginic acid, inoculating a liquid medium with the YIC-ALG3 saccharophilia bacillus and performing cultivation for certain time; adding the seed solution into the fermentation tank according to a certain mass ratio; performing fermentation at a certain condition; adjusting the fermentation condition after the fermentation and performing preservation for certain time; adjusting the fermentation condition again and performing replenishment with the seed solution; performing filter pressing after the adjustment is carried out for three times to obtain a saccharophilia bacillus filtrate; and adding water, the saccharophilia bacillus filtrate and potassium glutamate in a stirring tank, and performing stirring till the mixture is completely dissolved to obtain the liquid water-soluble fertilizer containing a saccharophilia bacillus agent and amino acid. The method is stable and controllable in fermentation process, short in fermentation period, and obvious in product effect, and is suitable for large-scale production.

The invention discloses a functional biological fertilizer which is prepared from 6 parts of isothiocyanate, 8 parts of ammonium molybdate, 11 parts of salicylic acid, 4 parts of zinc sulfate, 10 parts of silicon oxide, 4 parts of boric acid, 1 part of bacillus psychrosaccharolyticus, 4 parts of monopotassium phosphate, 5 parts of ethyl valerate, 4 parts of copper sulfate, 2 parts of manganese sulfate, 1 part of bacillus megatherium, 1 part of magnesium sulfate and 1 part of nitrobacteria. The functional biological fertilizer has the benefits that the fertilizer efficiency can be improved, insects can be prevented and treated, and the survival rate of microorganisms in the functional biological fertilizer can be also improved.

The invention discloses a preparation method of a biological organic fertilizer for loosening soil. The preparation method has the benefits that firstly, cheap organic materials are taken as main raw materials, and a microbial strain fermentation process is adopted for preparation, and a product has the characteristics of an organic fertilizer and the biological organic fertilizer and has better soil loosening and improving effects; secondly, bacillus psychrosaccharolyticus, nitrobacteria, EM bacteria and bacillus megatherium and other microorganism rotten strains are integrated step by step through a 'three-step fermentation method', so that the strain amplification and antagonism effects caused by simultaneous fermentation of multiple strains are avoided, the effective activities of the four strains are greatly retained, and the technical effects of the invention are obviously improved; thirdly, the production cost is low, the industrial production and the large-scale application are facilitated, and the defect of high cost existing in farmland soil compaction improved by the organic fertilizer and a soil conditioning agent product which are being sold in the current market is overcome.

The invention discloses a method for improving antioxidant ability of a trepang saponin extracting solution, and belongs to the technical field of marine organisms. Waste trepang internal organs obtained by deep processing of trepang are used as raw materials, and a modern bioseparation technique, a biological enzymolysis technique, a mixed bacterium microorganism fermentation technique and an ethanol precipitation and resin adsorption technique are integrated, so that the trepang saponin extracting solution high in in-vitro antioxidant ability and nutrient health-care efficacy is developed, wherein a compound enzyme preparation compound enzyme preparation is a mixture of one or more of proteases, non-starch polyase and pectinase, and mixed bacterium aerobic zymocyte mainly comprises candida utilis, bacillus licheniformis and bacillus psychrosaccharolyticus. The obtained trepang saponin has high antioxidant ability and high light spectrum properties, and can be efficiently utilized inthe fields of health care, foods, medicines and the like. The extraction method is high in extraction rate of the trepang saponin, can realize resource reusing of wastes obtained by processing of thetrepang, and is convenient for industrial production and broad in market prospects.

The invention relates to a Bacillus saccharophilus for degrading alginic acid and an application method thereof. The dried seaweed is ground into powder, and the seaweed powder is added into water according to a certain ratio, and after being put into a fermenter, at a certain temperature, a certain Sterilization under time; preparation of seed liquid: a fermentation strain YIC~ALG3 capable of degrading alginic acid was screened and inoculated into 2216E liquid medium for overnight cultivation; the seed liquid was added to the fermenter according to a certain mass ratio, and under certain conditions Fermentation, after fermentation, the raw material of brown algae is processed by microbial fermentation to prepare seaweed liquid. The fermentation process of the invention is stable and controllable, the fermentation period is short, the product has remarkable efficacy, and is suitable for large-scale production.

In the invention, a bacillus psychrosaccharolyticus obtained by screening is used for preparing an efficient complex microorganism seaweed fertilizer. The bacillus strain in the invention is named as bacillus psychrosaccharolyticus and preserved in the Common Microorganism Center under China Microorganism Strain Preservation and Management Committee with a preservation number of CGMCC No.4519. The bacillus psychrosaccharolyticus not only has the capacity of carrying out forced degradation on cellulose and starch but also has a wide antimicrobial spectrum; the bacillus psychrosaccharolyticus strain can be compounded with the traditional common strain for bacterial manure; then mixed zymocyte liquids, plant growth factors, immunoregulation factors, active extractives from seaweed, amino acids, organic matters and trace elements are prepared into an efficient complex mircoorganism seaweed fertilizer with an active viable count of 0.2 billion / g to 1 billion / g and an organic matter contentof 20-40% according to a certain proportion. The fertilizer has remarkable capacity in combating plant diseases and insect pests and promoting growth. With the fertilizer, the yield of crops can be increased by more than 30%.

The invention relates to a biological preparation for degrading pesticide residue in plants and a preparation method thereof, and relates to the technical field of agriculture production. The biological preparation solves the problems of poor pesticide degradation effects, low degradation speed and no broad spectrum of the existing microbial bacterial agents, and is prepared from a plant source pesticide mixture and a mixed bacterial solution according to the mixture ratio of 3:2, wherein the plant source pesticide mixture is prepared from the following materials in parts by weight: 1 to 5 parts of cortex meliae, 1 to 5 parts of radix sophorae falvescentis, 1 to 5 parts of hellebore and 1 to 5 parts of mustard seed, wherein the mixed bacterial liquid comprises the following ingredients inparts by weight: 20 to 30 parts of bacillus thuringiensis fermentation bacterial liquid, 10 to 15 parts of bacillus brevis lateralis fermentation bacterial liquid, 5 to 10 parts of bacillus psychrosaccharolyticus fermentation bacterial liquid, 5 to 10 parts of bacillus cereus fermentation bacterial liquid, 5 to 10 parts of yeast fermentation bacterial liquid, 5 to 10 parts of bacillus subtilis fermentation bacterial liquid, 5 to 8 parts of bacillus megatherium fermentation bacterial liquid, 30 to 35 parts of pseudomonas putidafermentation bacterial liquid and 25 to 30 parts of pseudomonas stutzeri fermentation bacterial liquid.

The invention belongs to the field of marine biotechnology, and discloses a method for improving the anticancer immunoenhancement efficacy of a Baolingsu extracted solution. Abalone is used as the rawmaterial, a modern biological separation technology, a biological enzymolysis technology, a mixed bacteria microbial fermentation technology, and an ethanol precipitation and resin adsorption technology are integrated to research and develop the Baolingsu biological product with high antitumor immunoenhancement efficacy, wherein mixed aerobic fermentation strains mainly comprise bacillus subtilis, bacillus licheniformis and bacillus psychrosaccharolyticus, and glucan is added in the enzymolysis process, so that the anti-cancer immunoenhancement efficacy of the Baolingsu extracted solution isremarkably improved. According to the process technology, the extraction rate of Baolingsu is high, and the obtained Baolingsu biological product is high in anti-cancer capability and convenient for industrial large-scale production.

The invention discloses a compounded fermented abalone-sea cucumber biological product and belongs to the technical field of marine organisms. The abalone-sea cucumber biological product disclosed bythe invention with high immunity strengthening capability and the effect of invigorating the kidney and supporting Yang is researched and developed through taking abalones and sea cucumbers as raw materials, assisting the raw materials with chitosan, and integrating a modern bioseparation technology, a bio-enzymolysis technology, a mixed-bacterium microbial fermentation technology and a marine biological resource nutrition blending technology; a blending ratio of the raw materials is as follows: 100 parts of abalones, 10-20 parts of chitosan and 50-80 parts of sea cucumbers; and mixed-bacterium aerobic fermentation strains mainly comprise bacillus psychrosaccharolyticus, bacillus subtilis and bacillus licheniformis. The abalone-sea cucumber biological product disclosed by the invention isrich in a variety of functional active substances such as functional polypeptides, glycosaminoglycan, flavone and saponin, has a remarkable immunity strengthening effect, has remarkable kidney invigorating and Yang supporting effects, is a novel efficient abalone-sea cucumber biological product and has a broad market application prospect.

The invention discloses a microbial rotten bacterium agent as well as a preparation method and an application thereof. The microbial rotten bacterium agent is formed by mixing and compounding bacillus psychrosaccharolyticus, geobacillus stearothermophilus and bacillus thuringiensis in proportion. The invention further provides the preparation method of the microbial rotten bacterium agent. Based on the optimization combination of the bacillus psychrosaccharolyticus, the geobacillus stearothermophilus and the bacillus thuringiensis, the rotten time is shortened by 10 to 20 days; the deodorization is carried out effectively during the rotten process, so that the environment pollution is avoided; the fatality rate of pests and worm eggs can reach 100%. The rotten agricultural / animal wastes or enteromorpha can directly serve as base fertilizers tilled into the soil, so that the soil structure is improved; the soil fertility is enriched; beneficial bacteria in the soil are increased. Thus, infectious diseases spreading via the soil are avoided.

The invention relates to a preparation method of a seaweed compound fertilizer containing a saccharophilous bacillus microbial inoculum. The preparation method comprises the following steps of grinding oven-dried seaweed into powder, adding the powder of the seaweed into water according to a certain proportion, loading an obtained first mixture in a fermentation tank, and then sterilizing the first mixture for a certain time at a certain temperature; the preparation of a seed solution: sieving to obtain a fermentation strain YIC-ALG3 saccharophilous bacillus capable of degrading alginic acid, inoculating the fermentation strain YIC-ALG3 saccharophilous bacillus into a liquid nutrient medium, and culturing the fermentation strain YIC-ALG3 saccharophilous bacillus for a certain time; adding the seed solution into the fertilization tank according to a certain mass ratio, fertilizing an obtained second mixture in a certain condition, after fertilization, adjusting a fertilization condition, preserving the second mixture for a certain time, then adjusting the fertilization condition once again, supplementing the seed solution, obtaining a saccharophilous bacillus after three times of adjustment, and adding the saccharophilous bacillus into a compound fertilizer through an external wrapping way, so as to obtain the seaweed compound fertilizer containing the saccharophilous bacillus microbial inoculum. According to the preparation method, the fertilization process is stable and controllable; the fertilization period is short; the function of a product is obvious; the preparation method is applicable to large-scale production.

The invention relates to a method for producing water-soluble fertilizer containing glycotropic bacillus and amino acid. Dried seaweed is ground into powder, the seaweed powder is added to water according to a certain proportion, and the mixture is placed in a fermentation tank and then sterilized at a certain temperature for a certain period of time; a seed solution is prepared; a fermentation strain YIC-ALG3 glycotropic bacillus capable of degrading alginic acid is obtained through screening, and a liquid culture medium is then inoculated with the fermentation strain for culture lasting a certain period of time; the seed solution is added into the fermentation tank according to a certain mass ratio to be fermented under a certain condition, the fermentation condition is adjusted after fermentation, the fermentation condition is adjusted again and the seed solution is supplemented after a certain period time of storage, glycotropic bacillus is obtained after three times of adjustment, and glycotropic bacillus and organic matter containing amino acid are evenly stirred to obtain the water-soluble fertilizer containing glycotropic bacillus and amino acid. The fermentation process is stable and controllable, fermentation period is short, product functions are remarkable, and the fertilizer is suitable for large-scale production.