Bacillus saccharophila used for degrading alginic acid, and application method thereof

A technology of Bacillus saccharophilus and alginic acid, which is applied in the field of Bacillus saccharophilus, can solve problems such as difficulty in meeting industrial production requirements, poor controllability of the fermentation process, and difficulty in guaranteeing product quality, and achieve remarkable product efficacy and stable and reliable fermentation process. The effect of control and short fermentation cycle

Active Publication Date: 2016-09-14
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are relatively few studies on the use of microbial fermentation to produce seaweed fertilizers. There are only a few studies that use a variety of microorganisms to perform composting or aerobic fermen

Method used

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  • Bacillus saccharophila used for degrading alginic acid, and application method thereof
  • Bacillus saccharophila used for degrading alginic acid, and application method thereof
  • Bacillus saccharophila used for degrading alginic acid, and application method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Grinding the washed and dried fresh sargassum into 50-60 mesh powder;

[0052] Seaweed powder and water are put into a fermenter according to the mass ratio of 2:100, and sterilized at 115°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate the above-mentioned Bacillus saccharophilus YIC~Alg3 into the liquid medium Cultivate for 20 hours; the preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogenphosphate 2g, ferrous sulfate 0.01 g. A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2-7.4; the volume ratio of Bacillus saccharophilus YIC-Alg3 to the culture medium is 2:100. Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 1:100 to obtain product B; ferment product B at a temperature of 20°C and a pH of 5.5 to 6.0, and the fermentation time is 7 days; collecting the ferment...

Embodiment 2

[0054] The washed and dried fresh copper algae and fresh sargassum are compounded according to the mass ratio of 1:1, and ground into 50-60 mesh powder;

[0055] Seaweed powder and water are put into a fermenter according to the mass ratio of 4:100, and sterilized at 115°C for 25 minutes to obtain product A; and prepare seed liquid: inoculate the Bacillus saccharophilus YIC~Alg3 into the liquid medium Cultivate for 22 hours; the preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01 g. A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2-7.4; the volume ratio of Bacillus saccharophilus YIC-Alg3 to the culture medium is 5:100. The seed liquid is added to product A, wherein the mass ratio of seed liquid to product A is 5:100, and product B is obtained; product B is fermented at a temperature of 25°C and a ...

Embodiment 3

[0057] Grinding the washed and dried fresh Sargassum into 50-60 mesh powder;

[0058] Seaweed powder and water are loaded into a fermenter according to the mass ratio of 6:100, and sterilized at 125°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate the Bacillus saccharophilus YIC~Alg3 into the liquid medium Cultivate for 24 hours; the preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01 g. A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2-7.4; the volume ratio of Bacillus saccharophilus YIC-Alg3 to the culture medium is 8:100. Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 4:100 to obtain product B; ferment product B at a temperature of 30°C and a pH of 7 to 7.5, and the fermentation time is 9 days ; Collecting the fermentation liquid ...

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Abstract

The invention relates to a Bacillus saccharophila used for degrading alginic acid, and an application method thereof. The application method comprises the following steps: 1, milling dried alga to form powder, adding water to the alga powder according to a certain ratio, filling a fermentation tank with the obtained solution, and sterilizing the solution at a certain temperature for a period of time; 2, preparing a seed liquid: screening a fermentation strain YIC~ALG3 capable of degrading alginic acid, inoculating the fermentation strain YIC~ALG3 to a 2216E liquid medium, and culturing the fermentation strain YIC~ALG3 overnight; and 3, adding the obtained seed liquid to the fermentation tank according to a certain mass ratio, fermenting the seed liquid under certain conditions, and processing a brown alga raw material through using microbial fermentation to prepare an alga liquid. The fermentation process is stable and controllable, the fermentation period is short, and the alga liquid has substantial efficacy, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a bacillus saccharophilus for degrading alginic acid, and also relates to a method for preparing seaweed liquid by using the bacillus saccharophilus and a method for preparing fertilizer by using the bacillus saccharophilus. Background technique [0002] Seaweed fertilizer is a new type of organic fertilizer that uses natural seaweed as raw material, undergoes special biochemical processing, extracts and retains the natural active ingredients of seaweed, refines and concentrates. Seaweed fertilizer has the effect of improving crop yield and quality, resisting pests and diseases and adverse environment, and improving soil and water conservation. Its application in agricultural production has huge market demand and good development prospects. However, in order to ensure the quality of seaweed fertilizer, two key links of seaweed raw material and extraction process must be grasped. [0003] First of all, the quality of seaweed fe...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G1/00C12R1/07
CPCC05B7/00C12N1/205C12R2001/07C05C3/00C05D1/02C05F5/00C05F5/008
Inventor 王明鹏王学江刘正一李峰秦松迟艳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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