300 results about "Bacillus polyfermenticus" patented technology

The invention relates to a composition containing fermented peony seed oil and application of the composition in skin care products, and belongs to the technical field of skin care products, the composition is composed of 20-25 wt% of fermented peony seed oil, 2-4.5 wt% of a saw palmetto fruit extract, 4 wt% of a bitter melon fruit extract, 1.5 wt% of an abelmoschus esculentus fruit extract, 0.5-1 wt% of an emulsifier and the balance of deionized water, the fermented peony seed oil is prepared through a fermentation process, and fermentation strains comprise lactobacillus plantarum and bacillus licheniformis. The composition containing fermented peony seed oil provided by the invention does not contain chemical components with high irritation or high skin burden, is mild and non-irritant, can effectively improve eczema, has a remarkable effect, and is suitable for being used as an active component to be added into a skin care product.

The invention provides a fermentation composition with an anti-oxidation effect as well as a preparation method and application of the fermentation composition. The composition is prepared by taking ginseng powder, corn stigma and chrysanthemum as fermentation raw materials and saccharomyces cerevisiae and bacillus subtilis as fermentation strains. Experiments show that the fermentation composition has a remarkable anti-oxidation effect, also has typical aroma characteristics, and is full and harmonious in aroma.

The invention discloses a high-efficiency aerobic fermentation corncob-based biological conditioner and application thereof. Aiming at the common problems of unstable carrier effect, poor environmental adaptability, easiness in inactivation of the microbial inoculum, difficulty in continuously exerting efficacy and the like existing in the existing composting microbial inoculum, the agricultural waste corncob with a porous structure and excellent water-retaining and fertilizer-retaining natural characteristics is pretreated and modified; and the immobilized carrier of the aerobic fermentation inoculant with high activity and high stability is constructed. The carrier matrix can be used for efficiently loading and remarkably improving the expanding culture core aerobic fermentation functional flora to prepare a solid biological conditioner. The corncob-based biological conditioner is applied to the composting process of organic solid wastes, promotes the humification process of organic materials, shortens the composting period, improves the fermentation efficiency of the materials, and effectively improves the resource utilization level of the organic solid wastes.

The invention discloses a method for preparing acid sophorolipid, and belongs to the technical field of biological fermentation. According to the method, candida is used as a fermentation strain, a composite carbon source culture medium containing a water-soluble carbon source and an oil-soluble carbon source is adopted, and the content of residual oil is monitored in real time in the fermentation process; when the residual oil content exceeds a threshold value of 5g / L, the emulsifying agent of 0.1 g / L-0. 5g / L is supplemented, and the stirring rotating speed is increased by 20-50rpm, so that the emulsification of an oil phase and the utilization of the thalli on the oil-soluble carbon source are promoted. By accurately controlling the residual oil content in the fermentation process, the proportion of the acidic sophorolipid and the carbon source conversion rate are remarkably increased, the proportion of the acidic sophorolipid is increased to 60% from 30% of a traditional process, the carbon source conversion rate is increased to 65% from 40%, the problems that the increasing means of the proportion of the acidic sophorolipid is single, and the carbon source is wasted are effectively solved, and the method is suitable for industrial production. The method has the advantages of simple process, low cost, environmental protection and the like.

The invention relates to a herbal acne-cleaning composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention relates to a herbal acne-removing composition. The composition comprises the following components in parts by weight: 1-3 parts of honeysuckle flower extract, 4-6 parts of mangnolia officinalis bark extract, 0.1-1 part of dandelion extract and 2-5 parts of fermentation extract, a fermentation strain used in the fermentation extract is lactobacillus fermentum, the preservation number of the lactobacillus fermentum is CGMCC No.21381, and fermentation substrate powder comprises amur cork powder and white willow bark powder. The herbal acne-removing composition disclosed by the invention has a remarkable acne-removing effect, and is mild and non-irritant.

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The invention discloses a preparation process of metabiotics and functional application of the metabiotics for relieving constipation, and relates to the technical field of microorganisms. The invention provides a preparation method of a bifidobacterium animalis subsp. Lactis LIHUO 01-containing metagen, which comprises the following steps: separately inoculating bifidobacterium animalis subsp. Lactis LIHUO 01 or compounding with probiotics to inoculate in a fermentation culture medium for culturing to obtain a fermentation bacteria solution, and carrying out thermal inactivation to obtain the bifidobacterium animalis subsp. Lactis LIHUO 01-containing metagen. The fermentation medium comprises skimmed milk, soybean meal and water, the mass ratio of the skimmed milk to the soybean meal to the water is (5-30): (1-25): (78-90), and the prepared bifidobacterium animalis subsp. Lactis LISUO 01 metabolite can significantly improve the content of tryptophan metabolites, can effectively relieve constipation and has a wide application prospect.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis, a fermentation agent containing the bacillus licheniformis, a preparation method of the fermentation agent, organic fertilizer, a preparation method of the organic fertilizer and a planting method of crops. The preservation number of the bacillus licheniformis is GDMCC No: 66520. The strain is only added as a zymophyte to compost the agaricus bisporus residues, so that the quick decomposition of the agaricus bisporus residues can be realized. And the decomposed product has a good promotion effect on crops, especially tomatoes.

The invention relates to the technical field of skin care product raw materials, and particularly discloses an antibacterial and antipruritic plant composition as well as a preparation method and application thereof. The preparation method of the antibacterial and antipruritic plant composition comprises the following steps: step 1) putting sanguisorba officinalis roots, pericarpium zanthoxyli fruits, gentiana australis, dracocephalum dracocephalum, artemisia capillaris and dogwood into water, and putting zymophyte for fermentation to obtain a fermented compound; (2) adding an ethanol water solution into the fermentation compound, heating, extracting, filtering, and taking filtrate to obtain a compound extracting solution; 3, the composite extracting solution is concentrated and dried, and the bacteriostatic and antipruritic plant composition is obtained, zymophyte is a compound of Weissella herlentini and zymozygous yeast.The bacteriostatic and antipruritic plant composition has the advantage that the bacteriostatic and antipruritic effect is further improved on the premise that low irritation is kept.

The invention belongs to the technical field of microorganisms, and particularly relates to a pseudomonas TaRb6 capable of preventing diseases and promoting growth and application of the pseudomonas TaRb6. The TaRb6 strain provided by the invention belongs to a potential new species of pseudomonas, and the preservation number of the TaRb6 strain is CGMCC (China General Microbiological Culture Collection Center) No.36131. The strain TaRb6 and a fermentation inoculant prepared from the strain TaRb6 have good disease prevention and growth promotion functions on plants; the bacterial inhibition rate of sterile fermentation liquor of the strain TaRb6 on fusarium pseudograminearum reaches 46.54%-62.82%, and the bacterial colony diameter of the fusarium pseudograminearum can be reduced by 61.38% through metabolized volatile organic compounds of the strain TaRb6. After the TaRb6 bacterial suspension is used for seed soaking treatment of wheat, the incidence rate of wheat basal stem rot can be remarkably reduced, and the growth vigor of wheat can be promoted. In addition, the strain TaRb6 has broad-spectrum antibacterial ability, and the antibacterial rate of the strain TaRb6 on pathogenic bacteria of fusarium, botrytis cinerea, apical cyst and xanthomonas reaches 47.23%-67.02%.

The invention discloses a preparation method and application of a recombinant A-type botulinum toxin, and aims to industrially produce the recombinant A-type botulinum toxin by utilizing the preparation method of the recombinant A-type botulinum toxin provided by the invention. The method comprises the following steps: firstly, constructing genetically engineered bacteria capable of heterologously expressing a botulinum toxin A gene in host bacteria by virtue of a genetic engineering means, then accumulating recombinant botulinum toxin A in a strain by virtue of the genetically engineered bacteria through high-density fermentation, collecting fermented thalli, crushing and purifying to obtain the recombinant botulinum toxin A, and finally, preparing the recombinant botulinum toxin A by virtue of the recombinant botulinum toxin A. And adding an auxiliary poising agent and a protective agent to prepare a liquid agent or a freeze-drying agent to obtain the recombinant A-type botulinum toxin for injection. According to the technical scheme, the complete recombinant botulinum toxin A with the purity as high as 99.9% is obtained, the yield is as high as 7000000 U / L, and the complete recombinant botulinum toxin A has no obvious difference from natural botulinum toxin A in structure and function and can be applied to multiple fields such as medical treatment, medical beauty and military affairs.

The invention provides a fruit juice fermentation inoculant, fermented guava juice and a preparation method, and belongs to the technical field of fermented beverages. The invention provides a compound fermentation inoculant capable of fermenting fruit juice, the compound fermentation inoculant is composed of lactobacillus plantarum and lactobacillus fermentum, the fermentation inoculant is applied to fermentation of guava fruit juice, beta-glucosidase secreted in the fermentation process can hydrolyze combined polyphenol substances in guavas, and then the guava fruit juice is fermented. The antioxidant is converted into high-activity antioxidants such as quercetin and gallic acid in a free state, so that the content of free phenol is increased by about 2 times, and the scavenging rate of DPPH free radicals is increased by more than 40%. The fermentation is carried out in an oxygen-free environment, the retention rate of vitamin C can reach 80% or above, the low pH value at the fermentation end point and organic acid jointly inhibit pathogenic microorganisms, and the product stability is guaranteed. Therefore, the guava juice prepared from the compound fermentation inoculant maximally releases the natural nutritional potential of guavas.

The invention discloses a fermentation material phellinus igniarius cultivation method, and relates to the technical field of phellinus igniarius cultivation.The phellinus igniarius cultivation method specifically comprises the following steps of compost preparation, composting fermentation, strain mixing, bagging spawn running and fruiting management.The invention further provides a culture medium bag for fermentation material phellinus igniarius cultivation. Sealed air bag strips are evenly distributed on the inner side of the packing material, a long-strip-shaped cushion material is arranged between the air bag strips, air holes are evenly distributed in the positions, corresponding to the cushion material, of the packing material, and after the packing material is filled with a fermentation phellinus igniarius culture base material, a fermentation material phellinus igniarius fungus stick is formed. A breathable growth groove used for mycelial growth is formed in the fermentation material yellow mulberry fungus stick; a lower cover body and an upper cover body are fixedly arranged at the two ends of the packing material respectively. The cost is reduced, nutrients are efficiently utilized, the air permeability is good, spawn running is accelerated, hypha activity is enhanced, the pollution rate is reduced, and the quality of sporocarp can be effectively improved.

The application discloses space Saccharomyces cerevisiae CEGF-TKJ-008, a roxburgh rose residue ferment and application thereof, the space Saccharomyces cerevisiae CEGF-TKJ-008 is preserved with the number CCTCC NO:M 2025504, the preserved bacteria have good acid resistance and acid reduction effect, can be used as roxburgh rose residue fermentation bacteria for fermenting roxburgh rose residue, and the obtained roxburgh rose residue ferment has the effect of stimulating appetite, can be applied to the preparation of roxburgh rose residue fermented feed with the effect of stimulating appetite, and is beneficial to improving the resource utilization value of roxburgh rose residue.

The invention discloses 10-hydroxydecanoic acid as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, Candida lipolytica is taken as a fermentation thallus, the Candida lipolytica contains alkane monooxygenase (CYP450), alcohol dehydrogenase (ADH), fatty alcohol oxidase (FAO) and fatty aldehyde dehydrogenase (FALDH), amplification and activation are performed on the Candida lipolytica in a seed culture medium, and the Candida lipolytica is obtained. The method comprises the following steps: inoculating a seed solution into a liquid culture medium, enabling a strain to adapt to a culture environment in advance, reducing strain death caused by environmental mutation after inoculation, inoculating the seed solution into the liquid culture medium, culturing until OD600 is 45-55, realizing high-density enrichment of the strain, being beneficial to increasing the content of CYP450, ADH, FAO and FALDH, adding decane and Tween 80, and enabling the Tween 80 to be capable of increasing the permeability of a cell membrane and increasing the reaction rate, the CYP450 is used for catalyzing decane to carry out hydroxylation, and ADH, FAO and FALDH are used for carrying out oxidation, so that the high-purity 10-hydroxydecanoic acid is obtained.

This invention discloses a strain of *Neocallimastix* LGM-ZA9 and its application in the preparation of a coumarin-degrading feed additive. *Neocallimastix* LGM-ZA9 is classified as *Neocallimastix* sp., with accession number CGMCC No. 17592, and its optimal growth temperature is 39℃. The fermentation broth of *Neocallimastix* LGM-ZA9 can be used to degrade the anti-nutritional factor coumarin. The method is simple: the coumarin-containing material to be degraded is mixed with the fermentation broth, sterilized, and then the fermentation broth is added for anaerobic fermentation to degrade the coumarin. This preparation method is simple, highly operable, and can significantly reduce the coumarin content, providing a new method for feeding ruminants with sweet clover as a high-quality forage.

The application provides a pediococcus acidilactici M4 for low-moisture fermented feed and an application thereof, and belongs to the field of microbial application and feed technology. The application uses the pediococcus acidilactici M4 to prepare fermented total mixed ration. The bacteria have the characteristics of high osmosis and fast acid production, and can reduce the pH value of the fermentation system in a short time; have strong broad-spectrum bacteriostatic effect on escherichia coli, chicken white dysentery salmonella and staphylococcus aureus; and perfectly adapt to the fermentation of low-moisture total mixed ration. The strain is used as the fermentation strain of low-moisture total mixed ration, can significantly reduce the content of acid detergent fiber and neutral detergent fiber, improve the crude protein level, and then improve the digestibility of the daily ration, increase the content of probiotics such as lactic acid bacteria and yeast in the daily ration, reduce the content of pathogenic bacteria such as escherichia coli in the daily ration, and also can improve the content of volatile fatty acid in the daily ration, improve the quality of the daily ration, and improve the feeding value of the daily ration.

The application belongs to the technical field of microbial fermentation, and particularly relates to a kind of cytolysis product of mixed bacteria fermentation and a preparation method. The cytolysis product is prepared by mixed bacteria fermentation of two kinds of aerobic probiotics, i.e. Lactobacillus plantarum and Aspergillus oryzae are used as fermentation strains to carry out mixed bacteria fermentation treatment on rice milk, and membrane filtration and column chromatography system are used for filtration and enrichment, so as to obtain a cytolysis fermentation product filtrate rich in kojic acid, the content of kojic acid is greater than 200 g / L, which is significantly improved compared with conventional process. The obtained cytolysis product has no bad smell and clear color, so that the beneficial function of kojic acid can be played only with a low addition amount in cosmetics, and the addition of odor masking and color correcting auxiliaries is reduced, and the cytolysis product can be used for preparing cosmetics with skin repair, whitening and other functions.

The invention discloses an areca-nut solid fertilizer for improving the content of catechin and precise nutrition of areca-nuts and application of the areca-nut solid fertilizer, and belongs to the technical field of fertilizer preparation. The preparation method comprises the following steps: mixing cassava straws, cassava residues, hermetia illucens sand, calcium superphosphate, quick lime and zymophyte, and carrying out first composting fermentation to obtain a first fermented product; and adding macroelements containing monopotassium phosphate, potassium nitrate, urea, calcium carbonate and magnesium sulfate in a specific proportion, microelements containing boron, zinc, copper, iron and manganese in a specific proportion and bacillus subtilis into the first fermented product, carrying out second composting fermentation, and carrying out aging treatment to obtain the betel nut solid fertilizer. The prepared betel nut solid fertilizer can accurately provide nutrients according to the requirements of betel nuts for various nutrient elements, and negative effects on soil due to excessive nutrient elements are avoided; meanwhile, the content of areca catechin is increased, and the nutritional value of the areca catechin is enhanced.

The present application relates to the technical field of feed processing, and particularly relates to a preparation method of Maotai-flavor distiller's grains fermented feed, which comprises the following steps: drying treatment based on the moisture content of Maotai-flavor distiller's grains to obtain basic distiller's grains; determining the strain ratio of brewer's yeast, bacillus subtilis and aspergillus niger based on the tannin content of the basic distiller's grains to obtain a compound fermentation inoculant; inoculating and then fermenting; determining state evaluation parameters based on the total polyphenol accumulation rate, the dissolved oxygen consumption rate and the microbial activity index during the fermentation process, and performing qualified evaluation, and dynamically adjusting when unqualified; determining the fermentation end point based on the tannin degradation rate and the total polyphenol accumulation rate; and mixing the fermented distiller's grains with the basic feed at a mass ratio of 1% after low-temperature drying of the fermented distiller's grains. The present application realizes three-layer control of raw material evaluation, process control and end point determination, significantly improves the stability and content of functional active ingredients in the product, reduces the tannin and safety risk, and has a good intestinal health improvement effect on animals.

This invention discloses a chitinase-producing Bacillus albus A01 and its application in the fermentation of shrimp and crab solid waste. The chitinase-producing Bacillus albus is named... Bacillus albus A01, with accession number CCTCC: M 20252571; the 16S rDNA gene sequence of this chitinase-producing Bacillus albus strain A01 is shown in SEQ ID NO.1. The fermentation agent of this strain can effectively degrade the chitin content in shrimp and crab solid waste, and improve the nutritional value of shrimp and crab fermented powder through solid-state fermentation. This invention solves the problem of unsatisfactory fermentation effects of existing feed fermentation strains, and has great application potential for improving the feed nutritional quality of shrimp and crab fermented powder and developing feed resources.

The invention belongs to the technical field of microorganisms, a streptococcus zooepidemicus mutant strain CGMCC (China General Microbiological Culture Collection Center) No.26991 is screened out, the streptococcus zooepidemicus mutant strain is obtained through screening by adopting a phage secondary infection method, resistance experiments and genetic stability experiments prove that the screened strain is an anti-phage strain, the resistance can be stably inherited, and the strain can be used as a high-yield streptococcus zooepidemicus mutant strain. The strain not only has good resistance to bacteriophages, but also has excellent high-yield hyaluronic acid characteristics, and can be used as an industrial fermentation strain of hyaluronic acid.

The application provides bacillus velezensis LB17, K87, MP6 and a compound microbial inoculant thereof, a preparation method and application, and belongs to the technical field of biocontrol bacteria.The application collects rhizosphere and rhizosphere soil of characteristic crops such as highland barley, lily, tomato and pepper in the Gansu-Qinghai region, and obtains 305 pure cultures through separation, obtains three strains of bacillus velezensis LB17, K87 and MP6 through multiple screening, and finally obtains a compound fermentation microbial inoculant, i.e., tuoweiwei No.7, which is fermented by the three strains of bacillus velezensis LB17, K87 and MP6 through optimization of inoculation amount, components of culture medium, ratio and fermentation conditions.Tuoweiwei No.7 can effectively prevent plant diseases such as pathogenic microorganisms, root rot and fusarium wilt, and can promote plant growth and improve the stress resistance of plants.The compound microbial inoculant provided by the application provides a strong guarantee for the prevention and control of root rot diseases of special habitat crops in the Gansu-Qinghai region.

The invention discloses a method for producing an efficient organic fertilizer by using kitchen waste, which comprises the following steps: draining the kitchen waste to obtain kitchen waste solid; cotton stalks and kitchen waste are mixed, and a compost material is obtained; the method comprises the following steps: culturing bacillus by using a T culture medium to obtain a zymophyte solution; mixing the zymophyte liquid with the compost material, adding water, and stirring and fermenting at the temperature of 60 DEG C; after reacting for 12 hours, adding the kitchen waste for the second time; after reacting for 24 hours, adding the kitchen waste for the third time; and reacting for 36 hours to obtain the efficient organic fertilizer. The preparation method of the organic fertilizer has the advantages that the treatment period is extremely short, the kitchen garbage is reduced by 85% within 36 hours, the product after the reaction does not need to be crushed, and the organic fertilizer product with the particle size of about 100 meshes is automatically generated; the method is energy-saving, environment-friendly and high in treatment efficiency, various indexes of the produced organic fertilizer can meet the agricultural industry standard of the People's Republic of China, namely the organic fertilizer, the economic and technical effects are remarkable, and industrial popularization and application are facilitated.

The application belongs to the technical field of microorganisms, and particularly discloses application of Bifidobacterium longum subsp. longum CGMCC No. 20105 in bacteriostasis. The application discloses for the first time that the Bifidobacterium longum subsp. longum strain CGMCC No. 20105 has the ability to ferment TPY or MRS to synthesize bacteriostatic substances, and the fermentation liquor has certain inhibitory effect on intestinal pathogenic bacteria, oral pathogenic bacteria, environmental pathogenic bacteria and antibiotic-resistant microorganisms. More importantly, the fermentation product does not affect the survival performance of conventional probiotics, indicating that the bacteriostatic substances synthesized by the Bifidobacterium longum subsp. longum strain CGMCC No. 20105 fermentation have certain safety, specificity and pertinence. Compared with other MRS fermentation strains, the bacteriostatic effect of the MRS fermentation liquor of the Bifidobacterium longum subsp. longum strain CGMCC No. 20105 is remarkable, and therefore has potential application value.

The present application relates to the technical field of fermented feed, and particularly relates to a compound feed based on aerobic-anaerobic combined fermentation, which comprises the following components in percentage by weight: fermented bacterial solution: 2-4%; Chinese herbal medicine additive: 0.5-1%; fermented base material: the balance; the preparation method of the compound feed comprises the following steps: S1, uniformly mixing the fermented bacterial solution and the fermented base material to obtain fermented material; S2, alternately performing aerobic-anaerobic combined fermentation according to the temperature of the fermented material to obtain pre-fermented material; S3, adding the Chinese herbal medicine additive, and alternately performing aerobic-anaerobic combined fermentation according to the temperature of the pre-fermented material to obtain the compound feed; the present application first promotes the proliferation of bacillus in a low-oxygen environment, decomposes macromolecular fibers and proteins, and accumulates metabolic products such as lactic acid, and then increases the ventilation frequency after heating to accelerate the release of active ingredients of the Chinese herbal medicine and improve the immunity of livestock and poultry.

The invention discloses a Kluyveromyces marxianus strain and an application of the Kluyveromyces marxianus strain, and belongs to the field of synthetic biology and microorganism manufacturing. The strain has the characteristics of efficiently utilizing xylose and growing under a low-oxygen condition, and by taking the strain as a fermentation strain, the protein content of fermented straw powder is remarkably improved through the synergistic effect of the strain and cellulase under a straw powder high-solid fermentation condition. According to the yeast strain and the high-solid fermentation process thereof disclosed by the invention, the protein content of straw powder can be greatly increased, and an effective solution is provided for high-valued reutilization of straw agricultural wastes.

The invention discloses a microbial compound fermentation inoculant, a straw fermentation product and application of the straw fermentation product, and belongs to the technical field of agricultural microorganisms. The preparation method comprises the following steps: compounding bacillus subtilis, trichoderma harzianum and trichoderma longibrachiatum to form a microbial compound fermentation inoculant; when the microbial compound fermentation inoculant is used for fermenting ginger straws, the content of pyrogallic acid in a prepared straw fermentation product can be synergistically improved. The pyrogallic acid has an obvious antibacterial effect on fusarium moniliforme and fusarium laminatum which cause the apple continuous cropping obstacle; the growth of apple seedlings planted in continuous cropping soil can be effectively promoted. Therefore, the straw fermentation product disclosed by the invention can be used for preventing and treating apple continuous cropping obstacles, improving the biomass and root activity of apple seedlings under continuous cropping conditions and reducing the quantity of harmful fusarium in continuous cropping soil.

The invention discloses a recombinant uricase mutant derived from arthrobacter globiforme as well as a purification method and application thereof, and belongs to the field of biological medicines. The mutant is mutated at 32-34 sites, 161 sites and 184 sites of an N end of recombinant uricase derived from arthrobacter globiforme, and the mutated amino acid sequence is as shown in SEQ ID NO: 1. On the basis of obtained uricase fermentation thalli, uricase is obtained through thalli splitting decomposition, centrifugation, nickel column affinity chromatography, liquid exchange, anion chromatography and liquid exchange, in the purification method, target protein uricase is enriched through nickel column affinity chromatography, then the purity of target protein can be increased to 95% or above through anion chromatography, and the purity of the target protein can be increased to 95% or above through anion chromatography. And finally, carrying out SUMO enzyme digestion to obtain the tag-free protein. The chromatography process is used for preparing uricase in an escherichia coli recombinant expression and purification mode, and the purity and yield of uricase are greatly improved.

This invention aims to address the technical bottlenecks of existing milk curd products, such as weak functional activity, unclear process parameters, and unstable flavor and quality. It utilizes *Pediococcus pentosaceus* HM4, *Streptococcus thermophilus* HM6, *Lactococcus lactis* JY4, and *Lactobacillus plantarum* NC1, employing these four strains in an optimal ratio as a compound fermentation agent to achieve synergistic effects. Using milk and goji berries as raw materials, the fermentation process of milk curds and goji berry milk curds was systematically optimized through single-factor experiments, Plackett-Burman experiments, and response surface methodology. The physicochemical indicators and flavor characteristics of the products under different process conditions were analyzed. The obtained milk curds achieved a DPPH free radical scavenging rate of 91.38%–94.15%, providing technical support for the high-value utilization of milk curds and enriching the variety of functional dairy products.