1094 results about "Bacillus polyfermenticus" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to a water improver with multiple active microbes and a preparation method thereof, which adopts two or a plurality of following microbial strains, comprising a bacillus licheniformis, a bacillus subtilis, a bacillus megaterium, a lactobacillus plantarum, a saccharomyces cerevisiae, a candida lipolytica, bacillus pumilus, bacillus cereus, a rhodopsedomnas palustris, an alcaligenes faecalis and an arthrobacter globiformis. After the femination of the microbial strains, the preparation of the liquid is that the single microzyme zymocyte liquid is mixed and stirred in proportion; or the mixed bacteria solution is concentrated; or the concentrated single microzyme zymocyte liquid is mixed and stirred according to the proportion of the single microzyme zymocyte liquid, and is prepared into a mix bacteria solution. The mix bacteria solution is mixed homogeniously with an added assistant agent. The total amount of the live bacteria within the warrenty period is 106 to 1010 per milliliter. The preparation of the solid inoculant is that the zymocyte liquid is dried after the absoption of the adsorbent on the zymocyte liquid, is prepared into a solid bacterial powder and mixed evenly with the added assistant agent. The total amount of the live bacteria within the warreny period ranges between 106 and 1011 per gram. The invention has multi-function and stable effect and is suitable for treating various water bodies.

The invention discloses a composite soil conditioner which belongs to the technical field of fertilizers, and mainly includes the following raw materials in parts by weight: 100-500 parts of humic acid with 45% of organic matter, 0.5-2 parts of ferrous sulfate, 8-100 parts of polyglutamic acid, 15-150 parts of polyaspartic acid, 1-50 parts of earthworm powder, 12-180 parts of shell powder, 10-50 parts of soybean cakes, 1-10 parts of amino-oligosaccharin, 10-30 parts of attapulgite powder and 10-20 parts of microorganism composite zymophyte. The composite soil conditioner provided by the invention can provide nutrition, adjust and balance the pH value of soil, adjust the soil microflora, improves the water retention, fertilizer maintenance and breathability of soil, degrade organic pollutants, releases phosphorus and potassium, has the antagonism function to heavy metals or can be used for precipitating heavy metals, reduces absorption of crops to heavy metals, and has the multiple conditioning functions of detoxifying the soil, building up fertility, improving the physical properties and micro-ecological environment of soil.

The invention discloses a fermented ginger and a manufacturing method thereof; the manufacturing method comprises the following steps that ginger is adopted as raw material, washed, cut into pieces or block, dried and sterilized; probiotic fermenting liquor is accessed into the ginger raw material, heat-preserved and fermented at the temperature of 28 DEG C to 39 DEG C; the used probiotics is lactobacillus, mould or bacillus spp, which can produce cellulase, amylase and protease, etc. and degrade cellulose, starch, protein and other macromolecular substances in the ginger; the invention adopts the probiotics as a zymogenic agent, and due to the existence of the probiotics, the growth of competitor is restrained in the processing, the storage period and the safety of the ginger is improved, the flavor of the ginger is improved, the nutrition of the ginger is strengthened, the bioactivity, the health and the utilization rate of the ginger are improved; the fermented ginger can be used as general seasoning, can also be used as the development of serial health foods with simple manufacturing process and stable result and is suitable for industrial production.

The invention belongs to the technical fields of feeds, enzyme preparations and microbial fermentation, and particularly relates to a preparation method of zymohydrolysis concentrated silage special for sows. The preparation method comprises the following steps of cutting short fresh pasturage or tuberous roots and stems, performing mashing, inoculating lactic acid bacteria, and performing natural enzymolysis and anaerobic fermentation so as to obtain silage; mixing one or more of cake meal, food processing by-products and cereals, with clean water and molasses syrup, inoculating lactic acid bacterium liquid, and performing natural enzymolysis and anaerobic fermentation so as to obtain fermented concentrated wet basis; and inoculating oxygen consumption fermentation bacterium liquid into a concentrated silage fermented substrate, and performing natural oxygen consumption fermentation so as to obtain the zymohydrolysis concentrated silage special for sows. The zymohydrolysis concentrated silage is rich in fermented cellulose lactic acid, calcium lactate, small peptide, amino acid, vitamins and probiotics, has the characteristics of being high in nutrition, easy to digest, good in palatability and the like, and can obviously increase the lactation quantity of the sows, obviously increase the food consumption and notably reduce the constipation of the sows.

The invention provides a new Lactobacillus plantarum bacterial strain YJG which was preserved in the China General Microbiological Culture Collection Center (CGNCC) with the collection number of CGMCC No. 2994 on march, 30th, 2009. The Lactobacillus plantarum bacterial strain YJG can produce broad-spectrum bacteriostatic activity bacteriocin with high yield, and can express bacteriostasis for staphylococcus aureus, salmonella and E.coli of gram-negative bacterium, L. plantarum, L.brevis, Streptococcus and Bacillus of gram-positive bacterium, and the like. A virus attacking tests with high dosage proves that the Lactobacillus plantarum bacterial strain YJG is safe to animal bodies. The Lactobacillus plantarum bacterial strain YJG and a zymophyte liquid thereof can be used for preparing additives of green animal feeds, and the bacteriocin of the Lactobacillus plantarum bacterial strain YJG can be used for preparing green biological veterinary drugs, environmental-friendly biopreservatives, biocontrol drugs and the like.

The invention discloses a low-temperature fermented milk as well as a raw material composition and a preparation method thereof. The raw material composition of the low-temperature fermented milk comprises raw material milk, a sweetener, a thickener, fermentation bacteria and 0.001%-0.006% of probiotics, and does not contain a preservative, wherein the percentage of the raw materials is the mass ratio of the raw material composition of the low-temperature fermented milk, and the probiotics are lactobacillus casei LC2W. The preparation method comprises the following steps: (1) uniformly mixing the raw material milk with the temperature of 40-45 DEG C, the sweetener and the thickener; (2) homogenizing emulsion obtained in the step (1) and pasteurizing; (3) cooling emulsion obtained in the step (2), uniformly mixing with the fermentation bacteria and the probiotics, and fermenting to obtain curd at the temperature of 35-42 DEG C; (4) overturning a vat for cooling, and pumping into a storage vat; (5) filling and sealing. According to the low-temperature fermented milk, the shelf life of the low-temperature fermented milk is remarkably prolonged, relatively high viable bacterium level is maintained, and meanwhile, the specific taste and nutrition of the fermented milk are kept.

The invention discloses a composite bacterial preparation as well as a preparation method and application thereof, which belong to the technical field of agricultural biochemistry. The composite bacterial preparation comprises the following components in parts by volume: 10-40 parts of brevibacillus brives fermentation bacterial liquid, 10-40 parts of pseudomonas fluorescens fermentation bacterial liquid and 15-45 parts of sugarcane thermophilic actinomyces, wherein the bacterial count in each mol of fermentation liquid is 0.4-0.7 billion. The strains of brevibacillus brives, pseudomonas fluorescens and sugarcane thermophilic actinomyce are separated from rabbit dung and fungi residues; and fermentation bacterial liquids of the separated strains are mixed according to a proper proportion to form the composite bacterial preparation. The composite bacterial preparation disclosed by the invention can be used for accelerating the degradation of organic materials and reducing the conversion of organic nitrogen into gaseous nitrogen, so that the composite bacterial preparation can present stronger ammonia-suppressing and decomposing capabilities in the fermentation process of pig manurecompost; and a smaller amount of ammonia gas is released while thorough decomposition of pig manure is accelerated.

The invention discloses a fermented low-salt semi-dried tilapia mossambica, a processing method thereof and application thereof. The processing method comprises the steps of: removing the internal organs and the scales of the tilapia mossambica; cutting along backside or abdomen; overturning; flatly pressing to form a symmetrical tilapia mossambica piece except the tail of the tilapia mossambica;washing with cleaning water; washing with salt water with 1%-4% of concentration to remove fishy smell and obtain the tilapia mossambica piece without the fishy smell; paving salt on the surface of the tilapia mossambica piece without the fishy smell, wherein the weight of the salt is equal to 3-8% of that of the tilapia mossambica; sousing for 8-24 hours; drying; or soaking for 8-24 hours with 5-10% of salt water to obtain the low-salt tilapia mossambica piece; spraying zymophyte on the surface of the tilapia mossambica piece, wherein the inoculum size of the zymophyte is 105-107 CFU / g fish meat; fermenting for 10-48 hours in a sealing way under the temperature of 12-20 DEG C; and drying the fermented tilapia mossambica piece to obtain the fermented low-salt semi-dried tilapia mossambicawith 30-60% of water content. The product has lubricating meat quality and typical flavor of dried meat. The processing method can be adapted to processing freshwater fishes.

The invention relates to the field of bioengineering, in particular to a preparation method and an application of a microbial agent composition for enhancing secondary fermentation of a bean paste. The microbial agent composition consists of a saccharomycetes agent, an aspergillus oryzae agent and a pleospora agent; and the three microbial agents are prepared, and then the prepared microbial agents are mixed with the secondary-fermented bean paste, so as to promote the secondary fermentation of the bean paste. The whole process of the preparation method of the microbial agent composition provided by the invention is applicable to continuous industrial production; by-products (chili and radix platycodonis), from the production of Pixian bean paste, are comprehensively utilized; a production cycle can be shortened by 6 months, the content of amino nitrogen can be improved by 20% and the content of volatile aroma-producing components can be improved by more than 3 times (the contents of total ester, total acid and total aldehyde); by inoculating aflatoxin B1 with the saccharomycetes agent at a production peak (after being fermented for 30-60 days), an ester producing and aroma generating process can be enhanced, the metabolism of aspergillus flavus and partial aspergillus parasiticus can be competitively inhibited and the content of the aflatoxin B1 can be reduced, and the content of the aflatoxin B1 is lower than 0.5ppm, so that food safety is enhanced.

The invention discloses an efficient microbial soil remediation agent, belonging to a propagation culture technique of microbes. The efficient microbial soil remediation agent is prepared by mixing the following components in percentage by volume: 10-25% of photosynthetic bacteria zymogen solution, 25-35% of Bacillus subtilis zymogen solution, 20-35% of microzyme zymogen solution and 20-40% of actinomycetes zymogen solution. The four strains are respectively subjected two secondary amplification culture under the conditions of proper temperature, ventilation, culture medium, pH value and culture time; and the culture process is carried out in a fermentation tank without foreign microbes. By using the modern biotechnology, single microbe strains are subjected to high-concentration pure culture and then compounded to produce the efficient microbial soil remediation agent.

The invention relates to a seeding medium made of vinasse methane-sludge and a preparation method thereof, not only solving the problem of environment contamination caused by vinasse methane-sludge, but also effectively utilizing vinasse methane-sludge. The seeding medium comprises fresh sludge containing 90 percent of water after methane generated from vinasse in a methane tank, accessories, ferment strain I and ferment strain II, wherein the accessories comprise grass peat, vermiculite and perlite; the ferment strain I comprises saccharomycetes, lactobacillus, high-temperature actinomycetes and bacillus; and the ferment strain II comprises photosynthetic bacteria, lactobacillus, bacillus, saccharomycetes, actinomycetes and terrestrial cyanobacteria. The preparation method comprises the following steps of preparing fresh sludge, dehydrating, adding the ferment strain I for fermentation, adding the ferment strain II for secondary fermentation, air drying, adding accessories, crushing, screening and packing. The vinasse methane-sludge contains 40 to 50 percent of organic matter and 4.3 percent of available nitrogen (N), phosphorus (P) and kalium (K). The seeding medium made of vinasse methane-sludge has better hydrophilicity, and seeding can be carried out after loading the dry seeding medium and spraying water, thus saving both labor and time.

A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-β-d-xylanase) and a β-glucanase (and optionally a further fibre degrading enzyme), wherein the DFM is selected from the group consisting of an enzyme producing strain; a C5 sugar-fermenting strain; a short-chain fatty acid-producing strain; a fibrolytic, endogenous microflora-promoting strain; or combinations thereof. The DFM may be selected from the group consisting of: Bacillus subtilis AGTP BS3BP5, Bacillus subtilis AGTP BS442, B. subtilis AGTP BS521, B. subtilis AGTP BS918, Bacillus subtilis AGTP BS1013, B. subtilis AGTP BS1069, B. subtilis AGTP 944, B. pumilus AGTP BS 1068 or B. pumilus KX11-1, Enterococcus faecium ID7, Propionibacterium acidipropionici P169, Lactobacillus rhamnosus CNCM-1-3698, Lactobacillus farciminis CNCM-1-3699, a strain having all the characteristics thereof, any derivative or variant thereof, and combinations thereof and the further fibre degrading enzyme may be selected from the group consisting of a cellobiohydrolase (E.C. 3.2.1.176 and E.C. 3.2.1.91), a β-glucosidase (E.C. 3.2.1.21), a β-xylosidase (E.C. 3.2.1.37), a feruloyl esterase (E.C. 3.1.1.73), an α-arabinofuranosidase (E.C. 3.2.1.55), a pectinase (e.g. an endopolygalacturonase (E.C. 3.2.1.15), an exopolygalacturonase (E.C. 3.2.1.67) or a pectate lyase (E.C. 4.2.2.2)), or combinations thereof.

The invention provide a kind additive product technique and purpose that can improve the enviroment of inner animal house, improve the animal fecundation index, lag rate ;survival rate, improve feed conversion rate, improve product rate, decline death rate decrease attibiuo tics utilization, improve economical efficient, improve character of meat, egg, mild, and can use for fermenting ensilage feed and feed. The additive of the invention mix with the different ratio fermentation liquid, such as photosynthetic bacterinm, bacillus, saccharomycote, lactic acid bacteria, actimomycete .

A method of producing ectoine by high-density culture of recombinant escherichia coli is provided. The method includes subjecting sodium L-aspartate to a biotransformation reaction by utilizing escherichia coli BW-p-BAD-ectABC having an accession number of CGMCC NO.8334. According to the method, 87.5 g of extracellular ectoine can be synthesized in each liter of fermenting bacteria after the fermenting bacteria are used repeatedly for five times, the synthetic efficiency is 11.67 g / L.d, and both the synthetic yield and the synthetic efficiency are higher than the reported synthesis levels. The method has great significance for industrial production and large-scale application of the ectoine.

The invention relates to a composite starter for sourdough steamed buns. The composite starter is a composition of fermentation strains including saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus brevis, wherein the bacterial number ratio of the saccharomyces cerevisiae to the lactobacillus plantarum to the lactobacillus brevis is (1 to 10): (1 to 5): 1. According to the composite starter for the sourdough steamed buns, which is disclosed by the invention, a strain is separated from the traditional sourdough, and a natural bacterium mixing system of the traditional starter is simulated, thus the traditional unique and abundant flavour can be given to the steamed buns, and the defect of insufficient flavour of the existing starter is made up; and the flavour and taste of the steamed buns can be improved, and the functions of increasing the nutritional value of the steamed buns and prolonging the shelf life of products are further realized due to the existence of lactic acid bacteria. The composite starter disclosed by the invention is simple in production procedures, the microbial inoculum is high in fermentation activity, and the steamed buns are stable in quality.

The invention discloses loop-mediated isothermal amplification (LAMP) primers, a kit and a detection method for detecting common carbapenemase genes of gram negative bacilli. In the LAMP primers disclosed by the invention, klebsiella pneumoniae carbapenemase (KPC) and new delhi metallo-b-lactamase (NDM) primer groups can detect all subtypes except for NDM-10; hypoxanthine nucleotide (IMP) and vimentin (VIM) primer groups can detect common subtypes at home and abroad. The LAMP kit built by the invention is applied to joint detection of KPC, NDM, IMP and VIM genes, can cover the common carbapenemase genes of non-fermentative bacteria and enterobacteriaceae, can accurately and quickly screen the common carbapenemase genes, and has great clinical significance for timely detecting and further controlling fulminant epidemic caused by propagation of the carbapenemase genes in enterobacteriaceae. The kit disclosed by the invention is high in detection sensitivity and the minimum detection limits of the KPC, NDM, IMP and VIM genes can reach 100 CFU / reaction.

The invention discloses a streptomyces parvus and application thereof for preparing daptomycin. The strain is reserved in the CCTCC and has the reservation number of CCTCC NO: M2010136, and the reservation data is June 4, 2010. The method for preparing the daptomycin is as follows: a, taking streptomyces parvus CCTCC NO: M2010136 as a fermentation strain; b, strain cultivation: inoculating slope lawn into a shake flask seeding tank to cultivate to obtain shake flask seeding liquid; inoculating the shake flask seeding liquid into the seeding tank to be cultivated; inoculating the seeding tank culture solution into fermentation tank culture medium to be cultivated, and collecting fermentation liquor; carrying out feed supplement in the fermentation and cultivation process; and c, extracting a fermentation product. The fermentation technology provided by the invention has stable production capability, high fermentation unit and few fermentation byproducts by the pilot plant test and the experiment in a 10-ton fermentation tank, greatly lowers post-extracting difficulty and is suitable for industrial production.

The invention belongs to the technical field of fermentation and relates to a solid-state fermentation method for the fermented bean pulp with a high digestibility rate. The solid-state fermentation method for the fermented bean pulp with a high digestibility rate comprises the following steps: fermenting strain liquid; evenly mixing strain liquid and the bean pulp; piling up in a fermentation tank; covering with a film; carrying out solid-state fermentation; drying; smashing; and packaging. The weight ratio of fermenting strains in the strain liquid is as follows: bacillus: lactic acid bacteria: saccharomycetes is (1.8-2.2): (0.8-1.2): (0.8-1.2); before the strain liquid and the bean pulp are evenly mixed, the strain liquid is heated at the temperature of 34-36 DEG C; the strain liquid and the bean pulp are mixed by a horizontal spiral ribbon mixer for 3-5min; the piling-up height of the fermentation tank is 40-60cm; and solid-state fermentation lasts for 3.5-5 days. In consideration of various factors which affect the digestibility rate of the fermented bean pulp, the invention determines the optimal large-scale and high-quality solid-state fermentation method for the fermented bean pulp.

The invention relates to a method for producing compound feed from mixed strains through united fermentation. According to the method, corn bran is taken as a main raw material and is subjected to batch culture through Aspergillus niger, trichoderma, bacillus subtilis, saccharomycetes and lactic acid bacteria, the microbiological feed is produced through combined conversion and solid fermentation, and the method comprises technological steps as follows: pretreatment of the raw materials, compounding of a mould starter, blending of yeast composite fermentation preparation, preparation of lactic acid bacteria starter, separate solid fermentation, drying, smashing and the like. According to the invention, the corn bran resource can be utilized sufficiently and efficiently, and the additional value of the corn bran resource is increased; harmful bacterial colonies in intestinal tracts can be reduced, the functions of conditioning healthcare, increasing the feed utilization rate, preventing diseases, promoting growth, reducing usage of drugs such as antibiotics and the like and improving the quality of livestock and poultry products are effectively performed, excrement stink is reduced, the breeding environment is improved, and one high-quality and low-cost green biological feed for feeding livestock and poultry is provided.

The invention discloses a method for producing bacterial cellulose by taking citrus pomace as a raw material, which belongs to the technical field of microbial fermentation and production. The method comprises the following steps: (1) pretreatment of the citrus pomace; (2) yeast fermentation of pretreatment liquid of the citrus pomace; (3) preparation of production and fermentation culture liquid; and (4) production and treatment of the bacterial cellulose, and the like. The citrus pomace is taken as the raw material, intermediate gluconacetobacter CIs26 CGMCC No.4663 which is autonomously selected and bred, the two-stage type fermentation process is adopted for producing the bacterial cellulose, the yield is as high as 13.5g / L, the production cycle is shortened from the conventional about half a month to 5-10 days, a product is semitransparent and good in flexibility, and the single situation of taking coconut milk as the raw material in the past is overcome. The method can be popularized and applied in bacterial cellulose production enterprises.

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

The invention relates to a microorganism composite zymocyte agent as well as a preparation method and an application thereof. The invention is characterized in that the active components of the composite zymocyte agent are Cida tropicalis and lactococcus lactis which are mixed according to the mass ratio of (0.5-2): 1. The preparation process comprises the following steps: carrying out oblique plane strain culture on the Cida tropicalis and lactococcus lactis; then carrying out liquid seed amplification culture to obtain a Cida tropicalis liquid and a lactococcus lactis liquid; mixing the Cida tropicalis liquid and lactococcus lactis liquid according to the mass ratio of (0.5-2): 1 and inoculating the mixture into a solid amplification culture medium to obtain the composite zymocyte agent according to the inoculum size of 4-5%(w / v); and adding the composite zymocyte agent to kitchen waste for microbial fermentation. The invention has the advantages of suppressing growth of putrefying bacteria in the kitchen waste and reproduction of pathogenic microorganism, thus featuring good freshness-retaining effect and organoleptic quality while increasing protein content and beneficial flora content of the kitchen waste.

The invention provides a composite bacillus preparation containing three strains, a preparation method of the composite bacillus preparation and application of the composite bacillus preparation to ecological breeding, and belongs to the technical field of microorganism and feed additives. Bacillus coagulans, butyric acid clostridia and bacillus megaterium are used as fermentative strains respectively, and large-scale, low-cost and high-density fermentation production of spores is conducted through step-by-step magnification; three live bacterium preparations are prepared by mixing bacterial sludge with the spores and dry starch; the three live bacterium preparations are matched according to the equal weight ratio, the composite bacillus preparation is obtained, and the concentration of the spores reaches more than 1*108-109 cfu / g; the composite bacillus preparation serves as a microorganism and feed additive to be added to farmed animal feed according to the mass ratio ranging from 0.02% to 0.5%. The composite bacillus preparation is simple and stable in process, low in cost, high in spore content and environment tolerance, easy to store and high in survival rate; the purposes of composition and synergism are achieved through the collaborative supplementary effects of different bacilli in the aspect of micro-ecology adjustment, growth of farmed animals is promoted, the feed utilization rate and feed rewards are increased, diarrhea is prevented and reduced, and the breeding ecological environment is improved.

The invention discloses a fodder zymophyte agent, a preparation method thereof and application thereof, which belong to the field of livestock cultivation. The microbial Chinese medicinal herb fodderzymophyte agent is prepared from the following raw materials in portion by weight: 20 to 40 portions of rice water, 0.3 to 0.5 portion of brown sugar and 60 to 80 portions of Chinese medicinal herbs.Through the microbial Chinese medicinal herb fodder zymophyte agent, the straw 'lignin' can be thoroughly denatured; the substances such as nodes, wax coats and the like which are not easy to be digested in the straws can be degraded into small molecular substances which are easy to be absorbed by animals through the decomposition function of microbial colonies; and the crude protein of the strawsis improved to 9 to 13 percent, the digestion rate reaches over 75 to 85 percent, the crude protein can be well absorbed by the animals, the weight of the animals is increased quickly, the fodder conversion rate is high, the daily gain and the milk yield are improved obviously, the meat quality is good, and the mouth feel is good. Moreover, the fodder zymophyte agent can kill all impurity bacteria, parasitic ovum and harmful components in the raw materials, ensure safety and innocuousness after the animals eat the fodder zymophyte agent, improve the immunity of the animals, and reduce the occurrence of epidemic diseases.

The invention discloses a fermented preparation method of vegetables, belonging to the field of vegetable deep processing, in particular to the field of vegetable fermented processing. The method comprises the following steps of: (1) adding materials and sealing; (2) carrying out primary fermentation; (3) fermenting after regulating temperature; and (4) seasoning. The fermented preparation method of vegetables is characterized in that: in the step of fermenting after regulating the temperature, fermentation is carried out after the temperature is controlled to be 15-30 DEG C for 30-90 hours, and meantime, the temperature is dropped to 0-5 DEG C at least once and maintained for 1-10 hours. In the invention, the fermentation is carried out via lactic acid bacteria, thus herba ixeris products have good flavor and are easy to store and eat, and the products are rich in probiotics and various nutrient components. When the method is used for producing herba ixeris pickles, manufacturers do not need to specially cultivate fermentation bacteria and build related cultivation equipment and facilities, thereby saving personnel and equipment investments.

The invention relates to a preparation method of a selenium enriched rhodopseudomonas palustris preparation, belongs to the technical field of microorganisms, and in particular relates to a liquid anaerobic fermentation method of the selenium enriched rhodopseudomonas palustris preparation. The preparation method comprises the following steps: by using rhodopseudomonas palustris as a fermenting strain, inoculating in an optimized liquid culture medium; cultivating for about 96 hours at 30-35 DEG C; after three-level amplified culture, respectively adding sodium selenite into 25, 50, 75 and 10 mu mg / mL on the second, third, fourth and fifth days; and continuously cultivating to the seventh day, wherein the concentration of selenium in the culture solution is decreased to about 10 mu mg / mL, the conversion ratio of selenium can reach over 90%, the selenium enrichment amount of the strain can reach 75mg selenium / g dry strain, and the organic selenium content is over 97%. The preparation provided by the invention has the advantages that massive fermentation of hodopseudomonas palustris can be completed by a common water white narrow-mouthed bottle and a polyethylene barrel, so that the preparation is simple to operate and low in energy consumption, thus, lowering the production cost and improving the production efficiency. The rhodopseudomonas palustris preparation prepared can be used as a feed additive for animals and further can provide foods rich in organic selenium for human kind.

The invention relates to a granular type compound microbial bacterial agent which comprises the following components in percentage by weight: 80-90 percent of powdered compound microorganism bacterial agent, 8-14 percent of phosphate rock powder and 2-6 percent of trace element, wherein the powdered compound microorganism bacterial agent is prepared by adsorbing zymocyte fluid with a single strain respectively with a medical stone, and the strains are bacillus subtilis, bacillus megateria and bacillus mucilaginosus. The invention also provides a preparation method and application of the granular type compound microbial bacterial agent. The invention provides a good carrier for the production of microbial bacterial agent granules; the medical stones have various characteristics of good adsorbability, leachability, mineralization, bioactivity, and the like and provide good carriers for the adsorption of the microbial bacterial agent; a produced fertilizer has better appearance, and the intensity of the fertilizer is obviously enhanced; and the invention greatly improves the utilization rate and the long-term effectiveness of the fertilizer and is beneficial to storing and transporting products.

The invention discloses microbial fermentation feed. The microbial fermentation feed is prepared from, by weight, 3-5 parts of a complex microbial agent, 680-730 parts of a fermentation raw material,275-325 parts of an energy raw material, 350-400 parts of water and 1-2 parts of brown sugar by mixed fermentation. The complex microbial agent is made by mixing of 5-6 of bacillus subtilis, enterococcus faecalis, plant lactobacillus, bacillus pumilus, clostridium butyricum, bacillus megaterium, bacillus amyloliquefaciens or lactobacillus casei. By fermentation treatment of the fermentation raw material and the energy raw material with the complex fermentation strain, enzymolysis of coarse fibers in the fermentation raw material is realized, organic nitrogen and inorganic nitrogen, which can be hardly used by animals, in the fermentation raw material are converted into bacterial protein, and macromolecular carbohydrates, which cannot be absorbed by the animal, in the energy raw material are converted into absorbable low-molecule carbohydrates.

The invention discloses a method for high-density culture of ready-to-use lactobacillus plantarum, freeze-dried powder prepared by the method and application. The method comprises the following steps: (1) placing freeze-dried powder of lactobacillus plantarum in an MRS culture medium for activation twice to obtain a seed starter, wherein the microbial content of the seed starter is 108-109 CFU / mL; (2) inoculating a high-density culture medium with the seed starter according to the volume ratio of (3-6):100 for fermentation culture at 36-38 DEG C, and collecting fermentation bacterial suspension 18-24 h later; (3) centrifugating the fermentation bacterial suspension at 6000-8000 rpm for 25-35 min, discarding supernatant, washing sediment with sterile saline, and collecting bacterial sludge; (4) adding a composite protective agent to the bacterial sludge according to the volume ratio of the composite protective agent to the bacterial sludge of (2-5): 1, and obtaining bacterial suspension containing the protective agent after uniform mixing; and (5) pre-freezing the bacterial suspension containing the protective agent at -20 DEG C to -65 DEG C for 1.5-6.0 h, and then conducting drying in a vacuum freeze-dryer for 18-24 h to obtain the freeze-dried powder. The viable count of the prepared freeze-dried powder is as high as 1010-1011 cfu / g, the moisture content of the freeze-dried powder is less than 3%, and the freeze-dried powder is not prone to agglomeration, has good rehydration performance and can be widely used.