821 results about "Bacillus polyfermenticus" patented technology

The invention relates to the technical field of health care products, and discloses kidney function health wine based on traditional Chinese medicine fermentation and a preparation method of the kidney function health wine. 6-10 parts of cistanche deserticola; 9-30 parts of radix astragali and 6-12 parts of fructus lycii; the fermentation strain comprises lactobacillus acidophilus, lactobacillus plantarum, bifidobacterium longum subsp. Longum, saccharomyces cerevisiae and bacillus subtilis, and the fermentation strain comprises the following strains: lactobacillus acidophilus JYLC-374, lactobacillus plantarum JYLC-482, bifidobacterium longum subsp. Longum JYBB-163, saccharomyces cerevisiae JYSC-710 and bacillus subtilis JYBS-312. By adopting the technical scheme that a traditional Chinese medicine compound formula is combined with specific probiotic fermentation, the technical effects of enhancing the bioavailability of active ingredients and promoting kidney function regulation are achieved, and the dissolution rate and the biotransformation rate of herbal medicine active substances are improved.

The invention relates to the technical field of agricultural microbial agents, in particular to bacillus velezensis as well as a separation method and application thereof. The bacillus velezensis is preserved in the China General Microbiological Culture Collection Center, the preservation date is May 17, 2024, the preservation number is CGMCC No.30687, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the postcode is 100101, and the bacillus velezensis is classified and named as bacillus velezensis. The bacillus velezensis separated from the soil of the Ningboo garden of the Academy of Agricultural Sciences in Yitai City of Shandong Province or the fermentation inoculant thereof can be simultaneously used as a preparation raw material of a growth promoting agent, a yield increasing agent, a soil conditioner and a bio-organic fertilizer, and has multiple application scenes.

According to the probiotic agent for enhancing the immunity and improving the intestinal flora and the application of the probiotic agent, five specific different types of bifidobacterium strains are compounded, potential interaction exists among the five strains, and the five strains can cooperate with one another, so that the effect of enhancing the immunity and improving the intestinal flora is achieved. The bifidobacteria has the advantages that the bifidobacteria has synergistic interaction in the aspects of promoting the growth of immune organs and increasing the total quantity and diversity of intestinal flora, the bifidobacteria is matched with basic fermentation strains for yogurt fermentation, meanwhile, the composite prebiotics are added for synergistic yogurt fermentation, the quantity of the bifidobacteria at the end of a shelf life after the yogurt fermentation is finished is still not less than 1 * 10 < 8 > CFU / mL or 1 * 10 < 8 > CFU / g, and the content of the bifidobacteria is not less than the stability of lactic acid bacteria in the yoghourt is remarkably improved, so that the number of viable bacteria which enter the intestinal tract of a human body and play roles is remarkably increased, and the yoghourt has relatively good tolerance in the intestinal tract; the prepared yoghurt is fine and smooth in texture, rich in taste and rich in fragrance; in addition, the immune decline and intestinal flora disorder caused by cyclophosphamide (CTX) can be remarkably improved, and a positive effect on immunological enhancement is achieved.

The invention discloses a method for preparing an agricultural biostimulant containing fructooligosaccharide from molasses and application of the agricultural biostimulant, and relates to the technical field of agricultural planting of fruits. The agricultural biostimulant is prepared by fermenting cane molasses with aspergillus oryzae or aspergillus niger, and the specific fermentation method comprises the following steps: inoculating activated strains into a cane molasses diluent with the baume degree of 10-16oBX, and carrying out aerobic fermentation on a shaking table at 25-35 DEG C for 70-72 hours to obtain a strain seed solution; and inoculating the strain seed solution into cane molasses diluent with the baume degree of 20-80 oBX, carrying out aerobic fermentation on a shaking table at 40-55 DEG C for 70-72 hours, and sterilizing to obtain the strain. By controlling the types of fermentation strains, the baume degree of cane molasses, the fermentation temperature and the fermentation time, the obtained fermentation product has the effects of promoting the growth and development of crops and increasing the yield, and is suitable for popularization.

The invention provides an efficient enzymolysis fermentation method for hermetia illucens larvae. The efficient enzymolysis and fermentation method for the larvae of the hermetia illucens comprises the following steps: a, selecting fresh or chilled larvae of the hermetia illucens, firstly cleaning and removing impurities, then pulping by adopting hydraulic pressure, and controlling the particle size within the range of 1-5mm after pulping so as to ensure the subsequent enzymolysis and fermentation effects; b, according to the characteristics of hermetia illucens larvae, selecting compound protease and neutral protease as main enzymolysis agents, and mixing the raw materials, the enzymolysis agents and water according to the mass ratio of 10: 2: 5. According to the efficient enzymolysis and fermentation method for the hermetia illucens larvae, by optimizing the enzymolysis reaction and fermentation process, the antioxidant activity of polypeptide prepared from the hermetia illucens larvae can be effectively improved. By accurately selecting enzymolysis agents such as compound protease and neutral protease and reasonably combining fermentation strains, the method can significantly improve the antioxidant effect of the final product, so that the method has higher market competitiveness when being applied to the feed industry.

The invention provides a Pichia megaterium BA1251 and a method for producing polyhydroxyalkanoate (PHA) by utilizing the Pichia megaterium BA1251 through semi-solid shallow tray fermentation. The Pichia megaterium BA1251 has the advantages that the Pichia megaterium BA1251 can be used for producing the polyhydroxyalkanoate (PHA) by the aid of the Pichia megaterium BA1251; the dry weight of zymophyte produced by the semi-solid fermentation method can reach 100g / kg, and the semi-solid fermentation method has the advantages of high yield, short period, low cost, less waste and environmental friendliness.

The invention discloses a Weissella sinus strain for improving the fermentation flavor of American ginseng and application of the Weissella sinus strain. According to the present invention, through the comprehensive evaluation of a variety of lactic acid bacteria, including the acid production capacity, the chroma regulation and the flavor characteristic analysis, the optimal strain for the American ginseng fermentation is finally screened, and the strain shows the significant flavor regulation advantage. On one hand, characteristic earthy smell substances of American ginseng can be completely degraded, and the bad flavor of the raw materials is effectively improved; on the other hand, a compound with a fruity flavor characteristic can be specifically generated, and a fermentation product is endowed with a remarkable fragrant and sweet flavor. In addition, the strain disclosed by the invention maintains proper acid production capacity and chroma stability, and the flavor quality is remarkably improved on the premise of ensuring the fermentation efficiency. Due to the characteristics, the strain becomes an ideal fermentation strain for developing a high-quality fermented American ginseng product.

A preparation method of a black fungus cultivation material containing pleurotus eryngii mushroom dregs comprises the following steps: adding the pleurotus eryngii mushroom dregs into dry chicken manure to form a fermentation base material, then spraying a tannic acid solution for pretreatment, then inoculating microorganisms for fermentation treatment to obtain pleurotus eryngii fermentation mushroom dregs, and then adding the fermentation base material into the fermentation base material for fermentation treatment to obtain the black fungus cultivation material containing the pleurotus eryngii mushroom dregs. Mixing the pleurotus eryngii fermentation mushroom dregs, wood chips, bran, soybean cake powder, gypsum powder and calcium superphosphate to form the cultivation material. Pleurotus eryngii fermentation mushroom residues are pretreated with a tannic acid solution with a specific concentration, then specific fermentation treatment is conducted, the obtained fermentation mushroom residues replace part of wood chips to prepare the cultivation material, the growth rate and growth vigor of black fungus hyphae are effectively increased, the full-bag period of the hyphae is shortened, and the yield of the black fungus hyphae is increased. Meanwhile, the growth of the black fungus sporocarp is promoted, the yield and quality of the black fungus are improved, the yield of the black fungus is improved by 11.29% compared with that of a cultivation material without pleurotus eryngii mushroom dregs, the content of auricularia auricula polysaccharide and amino acid in the black fungus is remarkably increased, and the breeding quality of the high-quality black fungus is effectively promoted.

The invention belongs to the technical field of plant drinks, and particularly relates to kidney-tonifying and bowel-relaxing cistanche tea and a preparation method thereof. The herba cistanche tea is prepared from the following raw materials in parts by weight: 30 to 40 parts of herba cistanche, 10 to 15 parts of fructus lycii extract, 10 to 12 parts of semen cassiae extract, 10 to 15 parts of fructus cannabis extract, 8 to 12 parts of radix polygoni multiflori extract, 8 to 10 parts of rhizoma dioscoreae extract, 3 to 5 parts of honey powder, 5 to 10 parts of maltodextrin, 2 to 3 parts of beta-cyclodextrin, 2 to 3 parts of antioxidant, 0.1 to 0.15 part of mixed enzyme and 2 to 3 parts of zymophyte. According to the preparation method disclosed by the invention, the flowers and the rhizomes of the cistanche are subjected to pretreatment and enzymolysis treatment in different modes, and then fermentation is performed under the same microbial fermentation condition, so that respective advantages of the two parts of raw materials are fully played, beneficial components are released and retained to the maximum extent, and the functionality and the quality of the product are remarkably improved through synergistic fermentation.

The invention discloses streptococcus salivarius subsp. Thermophilus JMCC0040 for preparing Greek yogurt and application of the streptococcus salivarius subsp. Thermophilus JMCC0040, and belongs to the technical field of microbial engineering. The streptococcus salivarius subsp. Thermophilus JMCC0040 strain is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 1, 2024, the preservation number is CGMCC NO.29835, and the Latin name is Streptococcus salivarius subsp. Thermophilus. The streptococcus salivarius subsp. Thermophilus JMCC0040 strain is named as Streptococcus salivarius subsp. Thermophilus. The streptococcus salivarius subsp. Thermophilus JMCC0040 for preparing the Greek yogurt, provided by the invention, has the advantages of good fermentation characteristic, high fermentation speed, weak post-acidity, low pink feeling of a fermented dairy product, high smoothness and good texture, and is suitable for preparing the Greek yogurt with higher requirements on fermentation strains.

The invention belongs to the technical field of food, and discloses a fruity Kangpu tea beverage and a preparation method thereof. The preparation method of the fruity Kangpu tea beverage comprises the following steps: S1, mixing tea sugar water, a candida utilis yeast seed solution and an acetobacter xylinum seed solution, and performing primary fermentation to obtain a primary fermentation solution; and S2, mixing the primary fermentation liquid, the fruit pulp, the candida utilis seed liquid and the lactobacillus plantarum seed liquid, carrying out secondary fermentation, and sterilizing to obtain the fruity Kangpu tea beverage. According to the method, fermentation is performed twice, specific zymophyte is adopted, phenolic substances and flavonoid substances in the tea leaves are fully released through glycolysis, the total acid content is reduced, and the fermentation period is shortened; meanwhile, the fruit pulp is added in the secondary fermentation process, so that the astringent taste and the unpleasant odor in the fermentation process are effectively covered, and the prepared Kangpu tea beverage is softer in taste, more layered in flavor and richer and fuller in taste.

The invention provides a lactobacillus plantarum KU01, and the preservation number of the lactobacillus plantarum KU01 is CGMCC (China General Microbiological Culture Collection Center) No.31788. The lactobacillus plantarum KU01 has the advantages that the preservation number is CGMCC No.31788; the invention also provides a fermentation product of the plant lactobacillus and a probiotic preparation comprising the plant lactobacillus and the fermentation product thereof. According to the probiotic agent and the preparation method thereof, the anti-helicobacter pylori probiotic agent is obtained through peracid stress fermentation, peracid stress fermentation, bacterial liquid separation, embedding, compounding and sieving. According to the method, on the basis of the inherent anti-helicobacter pylori physiological activity of the probiotics, the anti-helicobacter pylori physiological activity of the probiotics and the fermentation product is enhanced through stress fermentation, the fermentation product and the probiotics are sequentially released through an embedding technology, and an exogenous iron source is matched; and the anti-helicobacter pylori physiological activity is further promoted. The probiotics provided by the invention can be applied to resisting helicobacter pylori.

The invention discloses a selenium-rich bimorphic actinidia arguta enzyme as well as a preparation method and application thereof, and belongs to the technical field of microbial fermentation. The preparation method of the selenium-rich bimorphic actinidia arguta enzyme comprises the following steps: (1) performing selenium-rich culture on saussurea involucrata to obtain selenium-rich saussurea involucrata; (2) pulping the whole actinidia arguta fruits, performing enzymolysis, inoculating the selenium-rich saussurea involucrata bacteria, and performing fermentation culture; and (3) after the fermentation culture is finished, carrying out solid-liquid separation to obtain the selenium-rich solid-state actinidia arguta enzyme and the selenium-rich liquid-state actinidia arguta enzyme. The selenium-rich saussurea involucrata is used for whole-fruit fermentation of actinidia arguta for the first time, a bimorphic enzyme product is developed, the selenium content in the actinidia arguta enzyme is increased, and the enzyme flavor and antioxidant activity are enhanced. Compared with the prior art, the method has originality in the aspects of fermentation strains, culture modes, product forms and biological functions, and a whole set of processing technology which is simple in process and high in operability is provided.

The invention discloses a fermentation preparation process for reducing cold and cool characteristics of fructus forsythiae tea. The fermentation preparation process comprises the following steps: performing early-stage treatment of screening, weighing, cleaning, high-temperature softening, rolling and high-temperature steam sterilization on fructus forsythiae leaves; compounding lactobacillus plantarum and German-style lactobacillus bulgaricus to obtain a mixed strain, adding the mixed strain into the treated fructus forsythiae leaves, and putting into a fermentation box for solid-state fermentation; under a sterile condition, adding a fermentation strain of pichia guilliermondii BC 621 into the fructus forsythiae leaves subjected to solid-state fermentation, and putting into a fermentation box for secondary fermentation; and drying the fructus forsythiae leaves subjected to secondary fermentation until the water content is below 6%. Active components reflecting cold and cool characteristics in the fructus forsythiae tea prepared by the method are obviously reduced, the fructus forsythiae tea is also suitable for people with deficiency-cold spleen and stomach and deficiency-cold constitution to drink, and audiences are expanded.

The invention provides an efficient fermentation production method and application of acid type sophorolipid, and the production method comprises the following steps: (1) in a growth stage, adding a composite nitrogen source and growth factors into a basic culture medium to promote the growth of zymophyte and realize high-density fermentation, the zymophyte being candida utilis subjected to genetic engineering modification; (2) in the product synthesis stage, mixed syrup and vegetable oil are used as carbon sources, and the concentration of glucose in a fermentation tank is maintained at 1-5g / L and the concentration of residual oil is maintained at 1-5g / L by regulating and controlling the material supplementing rate. The fermentation production method provided by the invention solves the problems of slow thallus growth, long fermentation period, low production intensity, uncontrollable product composition, more byproducts, peculiar smell of fermentation tail gas and the like in the existing sophorolipid production process, and has industrial application value.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation substrate for bacillus, a bacillus liquid fermentation inoculant and application. The fermentation substrate is prepared by adding traditional Chinese medicine residues into a yeast culture medium so as to improve the concentration of bacillus after fermentation; the traditional Chinese medicine residues are prepared by compounding motherwort herb residues, astragalus membranaceus herb residues and ginseng herb residues according to the mass ratio of 1: (1-2): (1-1.5); the mass ratio of the yeast culture medium to the traditional Chinese medicine residues is (20-26): (6-8). The concentration of the bacillus can reach 3.3 * 10 < 9 > CFU / mL in a bacillus liquid fermentation inoculant obtained by adopting the fermentation substrate disclosed by the invention.

The invention discloses an Akkermansia massiliensis LTA21F2 strain and an application of the Akkermansia massiliensis LTA21F2 strain, and belongs to the technical field of microorganisms. The strain is preserved in the Guangdong Microbial Culture Collection Center on December 04, 2022, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 63035. The Akkermansia massiliensis anaerobic fermentation bacterial liquid provided by the invention is high in viable count and strong in oxygen tolerance, still has a survival rate of more than 90% after being exposed in air for 12 hours, the viable count in a sample is increased by 25.26% after being stressed by 1.5 M H2O2 for 2 hours, and the strain has a section of characteristic sequence which can be used as a specific molecular marker, and has the characteristics of high growth rate, high viable count and the like when being subjected to enlarged culture in a 200L fermentation tank.

The invention discloses a method for synthesizing rare ginsenoside by converting natural ginsenoside Rb1 with endophytic fungi separated from root rot ginseng. The method comprises the following steps: obtaining a Fusarium redolics c271F from root rot ginseng by utilizing a fungus separation method, carrying out conversion synthesis on a zymophyte of the Fusarium redolics c271F by taking ginsenoside Rb1 as a raw material to obtain rare saponins, and identifying the converted rare saponins as F2, Rg3 and CK on the basis of an HPLC (High Performance Liquid Chromatography) method. The method disclosed by the invention is simple to operate, green and safe, relatively high in yield and relatively low in cost, and a new method is provided for industrial production of the rare ginsenoside.

The invention provides a method for improving the conversion efficiency of fermentation protein for a cyperus esculentus feed and the prepared fermentation protein for the cyperus esculentus feed. Comprising the following steps: uniformly mixing cyperus esculentus cake meal, chitosan oligosaccharide and water, inoculating a fermentation strain, and culturing under proper conditions. Wherein the fermentation strain comprises saccharomycetes or a mixed strain of saccharomycetes and aspergillus niger; the saccharomycetes comprise saccharomyces cerevisiae or a mixed strain of the saccharomyces cerevisiae and candida utilis saccharomycetes. Saccharomyces cerevisiae is used as a main fermentation strain, sugar in fermentation raw materials can be fully utilized, mycoprotein is generated, and the protein content is increased; the candida utilis can utilize various carbon sources and nitrogen sources, so that the protein content and the thallus biomass are further improved; aspergillus niger can generate saccharifying enzyme, starch is hydrolyzed into glucose and other simple saccharides, and the saccharifying process is promoted. According to the method provided by the invention, the cyperus esculentus raw material with relatively low protein content can be converted into the cyperus esculentus feed fermentation protein, and the utilization way of the cyperus esculentus is expanded.

The invention relates to a composition containing fermented peony seed oil and application of the composition in skin care products, and belongs to the technical field of skin care products, the composition is composed of 20-25 wt% of fermented peony seed oil, 2-4.5 wt% of a saw palmetto fruit extract, 4 wt% of a bitter melon fruit extract, 1.5 wt% of an abelmoschus esculentus fruit extract, 0.5-1 wt% of an emulsifier and the balance of deionized water, the fermented peony seed oil is prepared through a fermentation process, and fermentation strains comprise lactobacillus plantarum and bacillus licheniformis. The composition containing fermented peony seed oil provided by the invention does not contain chemical components with high irritation or high skin burden, is mild and non-irritant, can effectively improve eczema, has a remarkable effect, and is suitable for being used as an active component to be added into a skin care product.

The invention belongs to the technical field of fermentation engineering, and particularly relates to turmeric meal fermented feed as well as a preparation method and application thereof. Iron, magnesium and zinc elements are added into fermentation base materials such as turmeric meal and soybean meal, and the mixture is fermented through a bacterium-enzyme synergistic fermentation inoculant to generate the turmeric meal fermented feed, wherein the fermentation inoculant comprises bacillus subtilis, lactobacillus plantarum and saccharomycetes. The prepared turmeric meal fermented feed can be applied to feed feeding of laying hens or broilers. Results of the embodiment of the invention show that the turmeric meal fermented feed prepared by the invention not only can effectively inhibit harmful bacteria in vitro, but also can remarkably improve the chicken production performance, the eggshell quality and the yolk color, and effectively improve the chicken quality of yellow feather broilers.

The invention provides a fermentation composition with an anti-oxidation effect as well as a preparation method and application of the fermentation composition. The composition is prepared by taking ginseng powder, corn stigma and chrysanthemum as fermentation raw materials and saccharomyces cerevisiae and bacillus subtilis as fermentation strains. Experiments show that the fermentation composition has a remarkable anti-oxidation effect, also has typical aroma characteristics, and is full and harmonious in aroma.

The invention discloses a high-efficiency aerobic fermentation corncob-based biological conditioner and application thereof. Aiming at the common problems of unstable carrier effect, poor environmental adaptability, easiness in inactivation of the microbial inoculum, difficulty in continuously exerting efficacy and the like existing in the existing composting microbial inoculum, the agricultural waste corncob with a porous structure and excellent water-retaining and fertilizer-retaining natural characteristics is pretreated and modified; and the immobilized carrier of the aerobic fermentation inoculant with high activity and high stability is constructed. The carrier matrix can be used for efficiently loading and remarkably improving the expanding culture core aerobic fermentation functional flora to prepare a solid biological conditioner. The corncob-based biological conditioner is applied to the composting process of organic solid wastes, promotes the humification process of organic materials, shortens the composting period, improves the fermentation efficiency of the materials, and effectively improves the resource utilization level of the organic solid wastes.

The invention discloses marasmius albus DCY6478 as well as a culture medium and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Marasmilla sp. DCY6478 strain, and the preservation number of the Marasmilla sp. DCY6478 strain is GDMCC (China General Microbiological Culture Collection Center) No.65883. The Marasmilla sp. Experimental verification results show that the culture medium formula most suitable for growth of the white marasmius is prepared from the following components in percentage by weight: 40% of apple sawdust, 36% of corncob, 14% of cottonseed hull, 1% of lime and 1% of gypsum; the fermentation liquor is verified to have an inhibition effect on a plurality of pathogenic bacteria; the DPPH free radical scavenging capacity of the fermented hyphae is verified, the superoxide dismutase activity and catalase activity are both higher than those of sporocarp, and the fermented hyphae can be used as a natural antioxidant source; it is verified that fermented hyphae or sporocarp of the strain have the capacity of secreting amino acid and polysaccharide and producing crude protein, and a new raw material selection is provided for the industries of food, medicine and the like.

The invention relates to the technical field of microbial fermentation, in particular to a fermented fish protein composition and a preparation process thereof. The preparation process of the fermented fish protein composition comprises the following steps: adding a substrate, compound zymophyte and the like into a fermentation container for fermentation; taking part of the fermentation liquor, heating for denaturation and low-temperature renaturation, adding the mesoporous silica carrier, and introducing into the fermentation container again; and continuously monitoring, and after fermentation is completed, carrying out solid-liquid separation and subsequent treatment on the fermentation liquid to obtain the fermented fish protein composition. The enzyme is immobilized through combination of heating denaturation and low-temperature renaturation, so that certain inhibitory structures in enzyme molecules can be broken, the enzyme is easier to interact with other molecules, the activity of the enzyme molecules is recovered, specific binding between the enzyme and the mesoporous silica carrier is promoted, and the immobilization efficiency is improved; the immobilized enzyme shows higher stability and activity, the service life is prolonged, and the loss of the enzyme is reduced, so that the fermentation efficiency is improved.

The invention discloses a novel anti-bacteriophage defense system which is named as an HXS system, and the nucleotide sequence of the novel anti-bacteriophage defense system is shown as SEQ ID NO.1. According to the invention, an anti-phage engineering bacterium is successfully constructed by using the system, and the modified strain has strong and wide defense capability to a plurality of phages. The invention provides a new technical approach for solving the problem of bacteriophage pollution of bacteria in industrial fermentation and biological pharmacy processes, and has wide application prospects in the aspects of bacteriophage pollution resistance in probiotics and zymophyte, domestication of efficient bacteriophage treatment preparations and the like.

The invention belongs to the technical field of food processing, and particularly relates to fermented beef jerky and a preparation method thereof. The preparation method of the beef jerky comprises the following steps: (1) removing fascia and fat from fresh cattle hind leg meat, washing with clear water, and cutting into meat slices; (2) adding table salt, glucose, white granulated sugar and sodium nitrite into the sliced meat, uniformly mixing, adding a fermentation inoculant which comprises staphylococcus calcaratus, pediococcus acidilactici, bifidobacterium bifidum and Witzia ciliaris, and fermenting; and (3) after fermentation, drying and cooling to obtain the fermented beef jerky. Specific strains are selected to form a fermentation inoculant for fermentation, and the prepared fermented beef jerky is high in free amino acid and soluble peptide content, low in nitrite residual quantity, uniform red brown in color and slightly bright in surface, and belongs to high-quality beef jerky.

The invention provides zero-fat low-sugar yoghourt and a preparation method thereof, the zero-fat low-sugar yoghourt comprises the following raw materials: skimmed milk, a fermentation inoculant, a sweetening agent and a protein product, the fat content of the skimmed milk is less than or equal to 0.3 g / 100mL, the protein content of the skimmed milk is greater than or equal to 6.0 g / 100mL, and the lactose content of the skimmed milk is less than or equal to 4.0 g / 100mL; the fermentation inoculant is composed of pichia kudriavzevii, lactobacillus helveticus and streptococcus thermophilus, and the ratio of the three strains is 2: 1: 5. According to the zero-fat low-sugar yoghourt disclosed by the invention, through selection of specific strains, good stability can be realized under the condition that a thickening agent and a stabilizing agent are not added, the product can be endowed with better oxidation resistance, human body metabolism improving efficacy and immunity regulating efficacy, and a green, clean and healthy yoghourt product is really obtained.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation strain composition, fermentation oil as well as a preparation method and application of the fermentation oil. The raw materials of the fermented oil comprise grease, candida bumblebee and a second strain; and the second strain is selected from at least one of lipomyces starkeyi, saccharomyces cerevisiae, rhodosporidium torulosporioides, lucid ganoderma and cordyceps sobolifera. The preparation method comprises the following steps: firstly, adding grease into a culture medium, inoculating bumblebee candida and a second strain, and fermenting; and sterilizing and filtering after fermentation. The invention provides a grease raw material fermentation strain combination capable of improving the grease stability, remarkably reducing the dosage of a grease emulsifier and improving the transdermal absorption effect of grease as an active matter carrier, and provides a basis for the application of the grease raw material fermentation strain combination in cosmetics.

The invention discloses a method for preparing acid sophorolipid, and belongs to the technical field of biological fermentation. According to the method, candida is used as a fermentation strain, a composite carbon source culture medium containing a water-soluble carbon source and an oil-soluble carbon source is adopted, and the content of residual oil is monitored in real time in the fermentation process; when the residual oil content exceeds a threshold value of 5g / L, the emulsifying agent of 0.1 g / L-0. 5g / L is supplemented, and the stirring rotating speed is increased by 20-50rpm, so that the emulsification of an oil phase and the utilization of the thalli on the oil-soluble carbon source are promoted. By accurately controlling the residual oil content in the fermentation process, the proportion of the acidic sophorolipid and the carbon source conversion rate are remarkably increased, the proportion of the acidic sophorolipid is increased to 60% from 30% of a traditional process, the carbon source conversion rate is increased to 65% from 40%, the problems that the increasing means of the proportion of the acidic sophorolipid is single, and the carbon source is wasted are effectively solved, and the method is suitable for industrial production. The method has the advantages of simple process, low cost, environmental protection and the like.