579 results about "Bacillus polyfermenticus" patented technology

The invention relates to the technical field of health care products, and discloses kidney function health wine based on traditional Chinese medicine fermentation and a preparation method of the kidney function health wine. 6-10 parts of cistanche deserticola; 9-30 parts of radix astragali and 6-12 parts of fructus lycii; the fermentation strain comprises lactobacillus acidophilus, lactobacillus plantarum, bifidobacterium longum subsp. Longum, saccharomyces cerevisiae and bacillus subtilis, and the fermentation strain comprises the following strains: lactobacillus acidophilus JYLC-374, lactobacillus plantarum JYLC-482, bifidobacterium longum subsp. Longum JYBB-163, saccharomyces cerevisiae JYSC-710 and bacillus subtilis JYBS-312. By adopting the technical scheme that a traditional Chinese medicine compound formula is combined with specific probiotic fermentation, the technical effects of enhancing the bioavailability of active ingredients and promoting kidney function regulation are achieved, and the dissolution rate and the biotransformation rate of herbal medicine active substances are improved.

The invention provides an efficient enzymolysis fermentation method for hermetia illucens larvae. The efficient enzymolysis and fermentation method for the larvae of the hermetia illucens comprises the following steps: a, selecting fresh or chilled larvae of the hermetia illucens, firstly cleaning and removing impurities, then pulping by adopting hydraulic pressure, and controlling the particle size within the range of 1-5mm after pulping so as to ensure the subsequent enzymolysis and fermentation effects; b, according to the characteristics of hermetia illucens larvae, selecting compound protease and neutral protease as main enzymolysis agents, and mixing the raw materials, the enzymolysis agents and water according to the mass ratio of 10: 2: 5. According to the efficient enzymolysis and fermentation method for the hermetia illucens larvae, by optimizing the enzymolysis reaction and fermentation process, the antioxidant activity of polypeptide prepared from the hermetia illucens larvae can be effectively improved. By accurately selecting enzymolysis agents such as compound protease and neutral protease and reasonably combining fermentation strains, the method can significantly improve the antioxidant effect of the final product, so that the method has higher market competitiveness when being applied to the feed industry.

The invention provides a Pichia megaterium BA1251 and a method for producing polyhydroxyalkanoate (PHA) by utilizing the Pichia megaterium BA1251 through semi-solid shallow tray fermentation. The Pichia megaterium BA1251 has the advantages that the Pichia megaterium BA1251 can be used for producing the polyhydroxyalkanoate (PHA) by the aid of the Pichia megaterium BA1251; the dry weight of zymophyte produced by the semi-solid fermentation method can reach 100g / kg, and the semi-solid fermentation method has the advantages of high yield, short period, low cost, less waste and environmental friendliness.

The invention discloses a Weissella sinus strain for improving the fermentation flavor of American ginseng and application of the Weissella sinus strain. According to the present invention, through the comprehensive evaluation of a variety of lactic acid bacteria, including the acid production capacity, the chroma regulation and the flavor characteristic analysis, the optimal strain for the American ginseng fermentation is finally screened, and the strain shows the significant flavor regulation advantage. On one hand, characteristic earthy smell substances of American ginseng can be completely degraded, and the bad flavor of the raw materials is effectively improved; on the other hand, a compound with a fruity flavor characteristic can be specifically generated, and a fermentation product is endowed with a remarkable fragrant and sweet flavor. In addition, the strain disclosed by the invention maintains proper acid production capacity and chroma stability, and the flavor quality is remarkably improved on the premise of ensuring the fermentation efficiency. Due to the characteristics, the strain becomes an ideal fermentation strain for developing a high-quality fermented American ginseng product.

The invention belongs to the technical field of food, and discloses a fruity Kangpu tea beverage and a preparation method thereof. The preparation method of the fruity Kangpu tea beverage comprises the following steps: S1, mixing tea sugar water, a candida utilis yeast seed solution and an acetobacter xylinum seed solution, and performing primary fermentation to obtain a primary fermentation solution; and S2, mixing the primary fermentation liquid, the fruit pulp, the candida utilis seed liquid and the lactobacillus plantarum seed liquid, carrying out secondary fermentation, and sterilizing to obtain the fruity Kangpu tea beverage. According to the method, fermentation is performed twice, specific zymophyte is adopted, phenolic substances and flavonoid substances in the tea leaves are fully released through glycolysis, the total acid content is reduced, and the fermentation period is shortened; meanwhile, the fruit pulp is added in the secondary fermentation process, so that the astringent taste and the unpleasant odor in the fermentation process are effectively covered, and the prepared Kangpu tea beverage is softer in taste, more layered in flavor and richer and fuller in taste.

The invention provides a lactobacillus plantarum KU01, and the preservation number of the lactobacillus plantarum KU01 is CGMCC (China General Microbiological Culture Collection Center) No.31788. The lactobacillus plantarum KU01 has the advantages that the preservation number is CGMCC No.31788; the invention also provides a fermentation product of the plant lactobacillus and a probiotic preparation comprising the plant lactobacillus and the fermentation product thereof. According to the probiotic agent and the preparation method thereof, the anti-helicobacter pylori probiotic agent is obtained through peracid stress fermentation, peracid stress fermentation, bacterial liquid separation, embedding, compounding and sieving. According to the method, on the basis of the inherent anti-helicobacter pylori physiological activity of the probiotics, the anti-helicobacter pylori physiological activity of the probiotics and the fermentation product is enhanced through stress fermentation, the fermentation product and the probiotics are sequentially released through an embedding technology, and an exogenous iron source is matched; and the anti-helicobacter pylori physiological activity is further promoted. The probiotics provided by the invention can be applied to resisting helicobacter pylori.

The invention discloses a selenium-rich bimorphic actinidia arguta enzyme as well as a preparation method and application thereof, and belongs to the technical field of microbial fermentation. The preparation method of the selenium-rich bimorphic actinidia arguta enzyme comprises the following steps: (1) performing selenium-rich culture on saussurea involucrata to obtain selenium-rich saussurea involucrata; (2) pulping the whole actinidia arguta fruits, performing enzymolysis, inoculating the selenium-rich saussurea involucrata bacteria, and performing fermentation culture; and (3) after the fermentation culture is finished, carrying out solid-liquid separation to obtain the selenium-rich solid-state actinidia arguta enzyme and the selenium-rich liquid-state actinidia arguta enzyme. The selenium-rich saussurea involucrata is used for whole-fruit fermentation of actinidia arguta for the first time, a bimorphic enzyme product is developed, the selenium content in the actinidia arguta enzyme is increased, and the enzyme flavor and antioxidant activity are enhanced. Compared with the prior art, the method has originality in the aspects of fermentation strains, culture modes, product forms and biological functions, and a whole set of processing technology which is simple in process and high in operability is provided.

The invention discloses a fermentation preparation process for reducing cold and cool characteristics of fructus forsythiae tea. The fermentation preparation process comprises the following steps: performing early-stage treatment of screening, weighing, cleaning, high-temperature softening, rolling and high-temperature steam sterilization on fructus forsythiae leaves; compounding lactobacillus plantarum and German-style lactobacillus bulgaricus to obtain a mixed strain, adding the mixed strain into the treated fructus forsythiae leaves, and putting into a fermentation box for solid-state fermentation; under a sterile condition, adding a fermentation strain of pichia guilliermondii BC 621 into the fructus forsythiae leaves subjected to solid-state fermentation, and putting into a fermentation box for secondary fermentation; and drying the fructus forsythiae leaves subjected to secondary fermentation until the water content is below 6%. Active components reflecting cold and cool characteristics in the fructus forsythiae tea prepared by the method are obviously reduced, the fructus forsythiae tea is also suitable for people with deficiency-cold spleen and stomach and deficiency-cold constitution to drink, and audiences are expanded.

The invention provides an efficient fermentation production method and application of acid type sophorolipid, and the production method comprises the following steps: (1) in a growth stage, adding a composite nitrogen source and growth factors into a basic culture medium to promote the growth of zymophyte and realize high-density fermentation, the zymophyte being candida utilis subjected to genetic engineering modification; (2) in the product synthesis stage, mixed syrup and vegetable oil are used as carbon sources, and the concentration of glucose in a fermentation tank is maintained at 1-5g / L and the concentration of residual oil is maintained at 1-5g / L by regulating and controlling the material supplementing rate. The fermentation production method provided by the invention solves the problems of slow thallus growth, long fermentation period, low production intensity, uncontrollable product composition, more byproducts, peculiar smell of fermentation tail gas and the like in the existing sophorolipid production process, and has industrial application value.

The invention discloses an Akkermansia massiliensis LTA21F2 strain and an application of the Akkermansia massiliensis LTA21F2 strain, and belongs to the technical field of microorganisms. The strain is preserved in the Guangdong Microbial Culture Collection Center on December 04, 2022, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 63035. The Akkermansia massiliensis anaerobic fermentation bacterial liquid provided by the invention is high in viable count and strong in oxygen tolerance, still has a survival rate of more than 90% after being exposed in air for 12 hours, the viable count in a sample is increased by 25.26% after being stressed by 1.5 M H2O2 for 2 hours, and the strain has a section of characteristic sequence which can be used as a specific molecular marker, and has the characteristics of high growth rate, high viable count and the like when being subjected to enlarged culture in a 200L fermentation tank.

The invention discloses a method for synthesizing rare ginsenoside by converting natural ginsenoside Rb1 with endophytic fungi separated from root rot ginseng. The method comprises the following steps: obtaining a Fusarium redolics c271F from root rot ginseng by utilizing a fungus separation method, carrying out conversion synthesis on a zymophyte of the Fusarium redolics c271F by taking ginsenoside Rb1 as a raw material to obtain rare saponins, and identifying the converted rare saponins as F2, Rg3 and CK on the basis of an HPLC (High Performance Liquid Chromatography) method. The method disclosed by the invention is simple to operate, green and safe, relatively high in yield and relatively low in cost, and a new method is provided for industrial production of the rare ginsenoside.

The invention relates to a composition containing fermented peony seed oil and application of the composition in skin care products, and belongs to the technical field of skin care products, the composition is composed of 20-25 wt% of fermented peony seed oil, 2-4.5 wt% of a saw palmetto fruit extract, 4 wt% of a bitter melon fruit extract, 1.5 wt% of an abelmoschus esculentus fruit extract, 0.5-1 wt% of an emulsifier and the balance of deionized water, the fermented peony seed oil is prepared through a fermentation process, and fermentation strains comprise lactobacillus plantarum and bacillus licheniformis. The composition containing fermented peony seed oil provided by the invention does not contain chemical components with high irritation or high skin burden, is mild and non-irritant, can effectively improve eczema, has a remarkable effect, and is suitable for being used as an active component to be added into a skin care product.

The invention provides a fermentation composition with an anti-oxidation effect as well as a preparation method and application of the fermentation composition. The composition is prepared by taking ginseng powder, corn stigma and chrysanthemum as fermentation raw materials and saccharomyces cerevisiae and bacillus subtilis as fermentation strains. Experiments show that the fermentation composition has a remarkable anti-oxidation effect, also has typical aroma characteristics, and is full and harmonious in aroma.

The invention discloses a high-efficiency aerobic fermentation corncob-based biological conditioner and application thereof. Aiming at the common problems of unstable carrier effect, poor environmental adaptability, easiness in inactivation of the microbial inoculum, difficulty in continuously exerting efficacy and the like existing in the existing composting microbial inoculum, the agricultural waste corncob with a porous structure and excellent water-retaining and fertilizer-retaining natural characteristics is pretreated and modified; and the immobilized carrier of the aerobic fermentation inoculant with high activity and high stability is constructed. The carrier matrix can be used for efficiently loading and remarkably improving the expanding culture core aerobic fermentation functional flora to prepare a solid biological conditioner. The corncob-based biological conditioner is applied to the composting process of organic solid wastes, promotes the humification process of organic materials, shortens the composting period, improves the fermentation efficiency of the materials, and effectively improves the resource utilization level of the organic solid wastes.

The invention discloses marasmius albus DCY6478 as well as a culture medium and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Marasmilla sp. DCY6478 strain, and the preservation number of the Marasmilla sp. DCY6478 strain is GDMCC (China General Microbiological Culture Collection Center) No.65883. The Marasmilla sp. Experimental verification results show that the culture medium formula most suitable for growth of the white marasmius is prepared from the following components in percentage by weight: 40% of apple sawdust, 36% of corncob, 14% of cottonseed hull, 1% of lime and 1% of gypsum; the fermentation liquor is verified to have an inhibition effect on a plurality of pathogenic bacteria; the DPPH free radical scavenging capacity of the fermented hyphae is verified, the superoxide dismutase activity and catalase activity are both higher than those of sporocarp, and the fermented hyphae can be used as a natural antioxidant source; it is verified that fermented hyphae or sporocarp of the strain have the capacity of secreting amino acid and polysaccharide and producing crude protein, and a new raw material selection is provided for the industries of food, medicine and the like.

The invention relates to the technical field of microbial fermentation, in particular to a fermented fish protein composition and a preparation process thereof. The preparation process of the fermented fish protein composition comprises the following steps: adding a substrate, compound zymophyte and the like into a fermentation container for fermentation; taking part of the fermentation liquor, heating for denaturation and low-temperature renaturation, adding the mesoporous silica carrier, and introducing into the fermentation container again; and continuously monitoring, and after fermentation is completed, carrying out solid-liquid separation and subsequent treatment on the fermentation liquid to obtain the fermented fish protein composition. The enzyme is immobilized through combination of heating denaturation and low-temperature renaturation, so that certain inhibitory structures in enzyme molecules can be broken, the enzyme is easier to interact with other molecules, the activity of the enzyme molecules is recovered, specific binding between the enzyme and the mesoporous silica carrier is promoted, and the immobilization efficiency is improved; the immobilized enzyme shows higher stability and activity, the service life is prolonged, and the loss of the enzyme is reduced, so that the fermentation efficiency is improved.

The invention belongs to the technical field of food processing, and particularly relates to fermented beef jerky and a preparation method thereof. The preparation method of the beef jerky comprises the following steps: (1) removing fascia and fat from fresh cattle hind leg meat, washing with clear water, and cutting into meat slices; (2) adding table salt, glucose, white granulated sugar and sodium nitrite into the sliced meat, uniformly mixing, adding a fermentation inoculant which comprises staphylococcus calcaratus, pediococcus acidilactici, bifidobacterium bifidum and Witzia ciliaris, and fermenting; and (3) after fermentation, drying and cooling to obtain the fermented beef jerky. Specific strains are selected to form a fermentation inoculant for fermentation, and the prepared fermented beef jerky is high in free amino acid and soluble peptide content, low in nitrite residual quantity, uniform red brown in color and slightly bright in surface, and belongs to high-quality beef jerky.

The invention provides zero-fat low-sugar yoghourt and a preparation method thereof, the zero-fat low-sugar yoghourt comprises the following raw materials: skimmed milk, a fermentation inoculant, a sweetening agent and a protein product, the fat content of the skimmed milk is less than or equal to 0.3 g / 100mL, the protein content of the skimmed milk is greater than or equal to 6.0 g / 100mL, and the lactose content of the skimmed milk is less than or equal to 4.0 g / 100mL; the fermentation inoculant is composed of pichia kudriavzevii, lactobacillus helveticus and streptococcus thermophilus, and the ratio of the three strains is 2: 1: 5. According to the zero-fat low-sugar yoghourt disclosed by the invention, through selection of specific strains, good stability can be realized under the condition that a thickening agent and a stabilizing agent are not added, the product can be endowed with better oxidation resistance, human body metabolism improving efficacy and immunity regulating efficacy, and a green, clean and healthy yoghourt product is really obtained.

The invention discloses a method for preparing acid sophorolipid, and belongs to the technical field of biological fermentation. According to the method, candida is used as a fermentation strain, a composite carbon source culture medium containing a water-soluble carbon source and an oil-soluble carbon source is adopted, and the content of residual oil is monitored in real time in the fermentation process; when the residual oil content exceeds a threshold value of 5g / L, the emulsifying agent of 0.1 g / L-0. 5g / L is supplemented, and the stirring rotating speed is increased by 20-50rpm, so that the emulsification of an oil phase and the utilization of the thalli on the oil-soluble carbon source are promoted. By accurately controlling the residual oil content in the fermentation process, the proportion of the acidic sophorolipid and the carbon source conversion rate are remarkably increased, the proportion of the acidic sophorolipid is increased to 60% from 30% of a traditional process, the carbon source conversion rate is increased to 65% from 40%, the problems that the increasing means of the proportion of the acidic sophorolipid is single, and the carbon source is wasted are effectively solved, and the method is suitable for industrial production. The method has the advantages of simple process, low cost, environmental protection and the like.

The invention relates to the field of microorganisms and discloses saccharomyces cerevisiae, a fermentation inoculant containing the saccharomyces cerevisiae, application of the saccharomyces cerevisiae and the fermentation inoculant and a method for producing fruit wine. The preservation number of the saccharomyces cerevisiae (Saccharomyces sp.) is CGMCC (China General Microbiological Culture Collection Center) No. 31571. The fermentation inoculant provided by the invention contains the saccharomyces cerevisiae. The invention also provides application of the saccharomyces cerevisiae and the microbial inoculum in production of ethanol and / or wine products. The saccharomyces cerevisiae strain provided by the invention has the properties of high glucose resistance, ethanol resistance, high salt resistance, high acid resistance, sulfur dioxide resistance and the like, has relatively strong ethanol production capacity and ester production capacity, can increase different flavors formed in a wine brewing process, and provides abundant strain resources for improving the flavor of sparkling wine.

The invention belongs to the field of fermentation strain screening, and discloses a high-throughput fermentation strain screening method based on artificial intelligence. Comprising the following steps: acquiring standard strains from a public strain preservation center, separating potential functional strains from edible mushroom residues, utilizing a high-throughput fermentation platform, setting different fermentation conditions, monitoring physical parameters in real time through an online sensor, constructing an artificial intelligence model, and quickly screening out dominant strains through real-time data acquisition and model prediction. Designing an optimization experiment scheme by using an AI performance prediction model, and according to a high-throughput experiment result, carrying out multi-round iterative optimization to obtain a single strain or a composite flora with optimal performance; according to the method, actual edible fungus residues are used as fermentation substrates, multiple groups of repeated experiments are set, strains or composite flora with the optimal performance are screened out, and intelligent screening of high-throughput fermentation strains is achieved.

The invention discloses a uric acid reducing composition containing eucommia polysaccharide as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The uric acid reducing composition prepared by the invention is prepared from the eucommia polysaccharide, the mulberry leaf extract and the clove extract, and the eucommia polysaccharide, the mulberry leaf extract and the clove extract are compounded for use, so that generation-excretion dual blocking can be formed aiming at different links of uric acid metabolism, and multi-target synergistic uric acid reduction is realized. Meanwhile, plant-derived components are adopted, so that the safety is higher, and adverse reactions can be effectively reduced. Besides, lactobacillus sake, lactobacillus plantarum and kluyveromyces marxianus are adopted as fermentation strains to prepare the mulberry leaf extract through fermentation, and the content of effective components in the mulberry leaf extract can be effectively increased, so that the uric acid reducing effect of the prepared composition is improved.

The invention belongs to the field of microbial preparations, and discloses a microbial stabilizer which comprises non-bacillus or a zymophyte solution thereof and a chemical stabilizer, and the non-bacillus comprises at least one of serratia nematophila and acinetobacter calcoaceticus. The invention also discloses a preparation method of the microbial stabilizer and an application of the microbial stabilizer in prolonging the shelf life of a microbial agent. The research on the microbial stabilizer containing non-bacillus discovers that the microbial stabilizer formed by proper matching of the non-bacillus and the chemical stabilizer can keep the stability of the concentration of viable bacteria for a long time, prolong the shelf life of microbial products and reduce the requirements of frequent production and storage; therefore, the production cost is directly reduced, the economic benefit is improved, and the method can be used in the technical field of microbial preparations.

The invention relates to a beauty and skin care enzyme and a preparation method thereof. According to the invention, fruits and traditional Chinese medicines are used as main fermentation raw materials, the fruits and the traditional Chinese medicines are crushed and mixed with fermentation strains, and active substances in each raw material are effectively retained through two-stage fermentation, filtration separation and freeze-drying treatment, SOD and sea buckthorn flavone contained in sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis, and the sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis. Salidroside contained in the rhodiola rosea has the skin care effects of resisting light aging and fatigue, astragalus polysaccharide and astragaloside contained in the astragalus membranaceus can repair a barrier and enhance immunity, ophiopogonin and polysaccharide contained in the radix ophiopogonis have the effects of preserving moisture and resisting saccharification, and hesperidin and nobiletin contained in the pericarpium citri reticulatae have the effects of resisting oxidation and adjusting grease secretion. The obtained compound enzyme has the effects of resisting oxidation, resisting inflammation and preserving moisture, has activity, stability and transdermal efficiency, and can be used in skin care products such as anti-oxidation essence, repair masks, late-up cream and the like.

The invention discloses fresh spirulina fermentation liquor with rose fragrance and a preparation method of the fresh spirulina fermentation liquor. The method is characterized in that 5% glucose, lactic acid bacteria, saccharomycetes and spore lactobacillus (10: 2: 1: 1) are adopted for composite fermentation of a bacterial liquid, dynamic pH regulation (4.5-6.0) and gradient fermentation (2-6 days) are combined, and synthesis of phenethyl alcohol flavor substances is promoted; the optimal fermentation process conditions are established, the phenethyl alcohol content reaches up to 547.52 mu g / kg, the oxidation resistance is remarkably improved, and the aroma and nutritional ingredients can be stably kept for 12 months under the refrigeration condition. According to the method, the problem that the fishy smell of algae is shielded is solved, and a novel aroma conversion path is constructed through strain metabolism regulation, so that the spirulina fermentation liquor has the natural flower fragrance and the nutrient enrichment characteristic. The method is low in cost, green, environmentally friendly and free of chemical additives, conforms to the development trend of healthy food, provides a new technical normal form for deep processing of algae, and has remarkable market competitiveness and industrialization value.

The invention discloses a straw decomposition bacterium, a fermentation bacterium agent and a straw stacking decomposition method, and relates to the technical field of biology. The invention relates to a straw decomposing bacterium (Ureibacillus masliensis) UM-1, which is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242457, the preservation date is November 7, 2024, and the strain has a 16S rDNA sequence as shown in SEQ ID NO: 1. The invention also discloses a fermentation bacterial agent containing the straw decomposition bacteria (Ureibacillus masliensis) UM-1, and a straw stacking decomposition method. The invention also discloses a fermentation bacterial agent containing the straw decomposition bacteria (Ureibacillus masliensis) UM-1. The straw decomposing bacterium (Ureibacillus massiliensis) UM-1 provided by the invention has the capability of degrading cellulose, hemicellulose and lignin, and has a good decomposing effect on rice straws and wheat straws.

The invention provides application of a bacillus subtilis strain Rhs-L31 in prevention and treatment of pine wood nematode disease, and relates to an agricultural environment microbial technology. In particular relates to a bacillus friaricola strain Rhs-L31 which is separated from rhizosphere soil of Chinese pines and application of the bacillus friaricola strain Rhs-L31 in prevention and treatment of pine wood nematode diseases. The strain is preserved in the China Center for Type Culture Collection in Wuhan University on December 25, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) 20242847. The in-vitro poisoning activity of the strain Rhs-L31 on pine wood nematodes can reach 100% within 24 hours; a fermentation filtrate and a zymophyte suspension of the strain Rhs-L31 have a remarkable inhibition effect on the propagation of the pine wood nematodes; the prevention and treatment effect of a zymophyte suspension of the strain Rhs-L31 on the pine wood nematode disease of the Chinese pine potted seedlings can reach 50%-72.5%; the bacillus subtilis strain Rhs-L31 disclosed by the invention has a good application prospect in the field of pollution-free prevention and treatment of pine wood nematode diseases.

The invention discloses a soil conditioner and a preparation method thereof, and belongs to the technical field of soil remediation. The preparation method of the soil conditioner comprises the following steps: preparing polyglutamic acid by using bacillus subtilis and bacillus natto as fermentation strains, continuously adding sterilized straw powder after fermentation is completed, carrying out secondary fermentation, drying the obtained fermentation product, and mixing the dried fermentation product with decomposed straw and ardealite to obtain the soil conditioner. The soil conditioner provided by the invention has the advantages of simple preparation steps and cheap and easily available raw materials. The prepared soil conditioner has a good improvement effect on saline-alkali soil. The saline-alkali soil improved by the soil conditioner provided by the invention cannot stress the growth of crops due to salt and alkali, the improved soil has high organic matter content and lasting fertility, and the yield of the crops can be obviously increased. After the soil conditioner provided by the invention is applied, the required irrigation and drainage amount is small, and the removal rate of harmful anions and cations in the saline-alkali soil is high. The soil conditioner and the preparation method thereof provided by the invention are high in economic value and have wide popularization and application prospects.

The invention relates to a herbal acne-cleaning composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention relates to a herbal acne-removing composition. The composition comprises the following components in parts by weight: 1-3 parts of honeysuckle flower extract, 4-6 parts of mangnolia officinalis bark extract, 0.1-1 part of dandelion extract and 2-5 parts of fermentation extract, a fermentation strain used in the fermentation extract is lactobacillus fermentum, the preservation number of the lactobacillus fermentum is CGMCC No.21381, and fermentation substrate powder comprises amur cork powder and white willow bark powder. The herbal acne-removing composition disclosed by the invention has a remarkable acne-removing effect, and is mild and non-irritant.

The invention belongs to the technical field of bioengineering, and particularly relates to kluyveromyces marxianus engineering bacteria for synthesizing eicosapentaenoic acid and application of the kluyveromyces marxianus engineering bacteria. According to the engineering bacterium, pyruvate decarboxylase PDC1 genes, alcohol dehydrogenase ADH1 genes and alcohol dehydrogenase ADH2 genes are sequentially knocked out from a kluyveromyces marxianus FIM1 delta ura3 strain; meanwhile, a citrate lyase ACL gene, a pyruvate oxidase PO gene and a phosphate transacetylase PTA gene are inserted into the loci respectively, so that synthesis of acetyl coenzyme A is enhanced; then a plurality of genes are sequentially inserted into a Kluyveromyces marxianus genome, and then a vector of a delta 8 desaturase delta 8D gene is transferred into the Kluyveromyces marxianus genome. The invention also provides a method for efficiently preparing the EPA, namely the Kluyveromyces marxianus engineering strain is used as a fermentation strain, glucose, a yeast extract, peptone and other components are used as a culture medium, and the yield of the EPA produced by culturing and fermenting reaches 8.71 mg / g of dry cell weight.