234 results about "Sophorolipid" patented technology

A bioleaching composition for treating ores or other materials including metals or metal salts is presented, wherein the bioleaching composition includes a biosurfactant and a metal solubilizing agent. The biosurfactant may be a sophorolipid biosurfactant (SLP), and the metal solubilizing agent includes an acid and an oxidizing agent or a microorganism. In a bioleaching process using the bioleaching composition, the metal in the ore may be separated from a solution phase or a non-solution phase. Compositions derived from microorganisms are also provided for the pre-leaching treatment of a metal source, which compositions can be used to reduce impurities in the metal source. The use of the bioleaching composition increases the extraction rate and / or the throughput of the process.

The invention discloses a kitchen waste oil-based sophorolipid high-yield candida utilis strain and an application thereof. Belongs to the technical field of directional domestication breeding. According to the method, wild candida bombicola is used as an original strain, and is sequentially domesticated in a culture medium containing kitchen waste oil with the concentration gradient of 2%, 4%, 6%, 8% and 10% to obtain the S.bombicola x10. The invention further discloses an application of the domesticated strain in fermentation production of sophorolipid, after the strain is subjected to shake flask fermentation, the sophorolipid yield reaches 60.19 g / L and is improved by 16.22% compared with that of a wild strain, and the acid type sophorolipid yield is improved by 32.11%. When the concentration of the lactone sophorolipid generated by fermentation is 1.25 mg / mL, the growth of the bacillus subtilis is completely inhibited. The invention provides an excellent production strain for industrial production of sophorolipid, and has good theoretical research significance and practical application value.

The invention provides an efficient fermentation production method and application of acid type sophorolipid, and the production method comprises the following steps: (1) in a growth stage, adding a composite nitrogen source and growth factors into a basic culture medium to promote the growth of zymophyte and realize high-density fermentation, the zymophyte being candida utilis subjected to genetic engineering modification; (2) in the product synthesis stage, mixed syrup and vegetable oil are used as carbon sources, and the concentration of glucose in a fermentation tank is maintained at 1-5g / L and the concentration of residual oil is maintained at 1-5g / L by regulating and controlling the material supplementing rate. The fermentation production method provided by the invention solves the problems of slow thallus growth, long fermentation period, low production intensity, uncontrollable product composition, more byproducts, peculiar smell of fermentation tail gas and the like in the existing sophorolipid production process, and has industrial application value.

The invention relates to an oligomeric bio-based surfactant and a preparation method and application thereof, the oligomeric bio-based surfactant has a structure as shown in the following formula 1 or formula 2: # imgabs0 # imgabs1 # X1-X2 are selected from-(CH2) n-or substituted or unsubstituted thienyl with the carbon atom number of 4-10, n represents the number of CH2, and n is any integer selected from 0-12; z1-Z5 are respectively and independently selected from a disaccharide group, R1-R5 are respectively and independently selected from-CH (CH3)-Y-COOH, and Y is selected from a saturated or unsaturated linear alkyl group with the carbon atom number of 9-19. Compared with unmodified sophorolipid, the sophorolipid is modified. The oligomeric bio-based surfactant disclosed by the invention has a lower contact angle and higher wettability; meanwhile, molecules have a branched space structure and intramolecular sugar ring steric hindrance, fusion of foams can be promoted, the low-foam characteristic is shown, and the low-foam bio-based cleaning agent has a good industrial application prospect as a green and efficient low-foam bio-based cleaning agent.

The invention relates to a biological long-acting bactericide and a preparation method and application thereof.The biological long-acting bactericide can release a quaternary ammonium salt type sophorolipid sterilization unit with the sterilization effect according to needs, the sterilization time is effectively prolonged, the recycling performance of the bactericide is improved, and the biotoxicity of the bactericide is reduced; the excessive use of the bactericide is avoided to a great extent; in addition, the biological long-acting bactericide also has excellent substrate adhesion and washing resistance, and is beneficial to inhibition of adhesion of bacteria on the surface of a material, so that the surface of the material washed by water still keeps relatively high antibacterial ability.

The invention discloses a method for purifying modified sophorolipid, which comprises the following steps: adding a salt solution into lactone type crude sophorolipid obtained by fermenting candida bumblebee, stirring, standing, and collecting a lower layer sophorolipid phase; according to the method, the produced crude sophorolipid with low or high emulsification degree is purified, impurities are removed, good appearance conditions are achieved, and the appearance requirement of the commodity sophorolipid is met. And meanwhile, the sophorolipid is modified in the purification process by utilizing the chemical properties of different salts, so that the decoloration of the sophorolipid is realized, the concentration of the lactone type sophorolipid is improved, and the stability of the lactone type sophorolipid is improved at the same time.

The invention discloses a method for producing feed by using corn sugar residues, and belongs to the technical field of fermentation. The method comprises the following steps: pretreating, proportioning, adding an antibacterial agent, fermenting, drying and crushing, coating, cooling and the like, mixing corn sugar residues, corn germ meal and threonine mother liquor according to a specific ratio, adding zinc oxide and a chitosan antibacterial agent, and inoculating Saccharomyces cerevisiae ATCC 9763 for fermenting. And performing multi-stage drying and crushing on the fermentation product, spraying a coating emulsion prepared from a defatted soybean meal extract, curcumin, vitamin E, lecithin and sophorolipid, and performing air cooling to prepare the feed. According to the method, by-products are fully utilized, the crude protein content of the feed is 32-34.5%, the diameter of an inhibition zone is 18.1-20.2 mm, the moisture absorption rate is 2.7-3.4%, the palatability score is 8.0-8.6, and the fermentation yield is 92-94%. The innovation point lies in specific strain fermentation and functional coating, and is suitable for industrial production.

The invention discloses a method for preparing acid sophorolipid, and belongs to the technical field of biological fermentation. According to the method, candida is used as a fermentation strain, a composite carbon source culture medium containing a water-soluble carbon source and an oil-soluble carbon source is adopted, and the content of residual oil is monitored in real time in the fermentation process; when the residual oil content exceeds a threshold value of 5g / L, the emulsifying agent of 0.1 g / L-0. 5g / L is supplemented, and the stirring rotating speed is increased by 20-50rpm, so that the emulsification of an oil phase and the utilization of the thalli on the oil-soluble carbon source are promoted. By accurately controlling the residual oil content in the fermentation process, the proportion of the acidic sophorolipid and the carbon source conversion rate are remarkably increased, the proportion of the acidic sophorolipid is increased to 60% from 30% of a traditional process, the carbon source conversion rate is increased to 65% from 40%, the problems that the increasing means of the proportion of the acidic sophorolipid is single, and the carbon source is wasted are effectively solved, and the method is suitable for industrial production. The method has the advantages of simple process, low cost, environmental protection and the like.

Compositions having at least one biosurfactant and at least one persicomycin. The at least one biosurfactant is selected from the group consisting of rhamnolipids, glucolipids and sophorolipids. The compositions optionally have at least one enzyme, at least one non-biosurfactant and / or at least one selected from the group consisting of an anti-redeposition polymer and a soil release polymer. The compositions clean surfaces of an article in a process for cleaning said article.

The invention discloses a three-sludge treatment agent and a preparation method thereof, and belongs to the technical field of oily sludge treatment, the three-sludge treatment agent comprises a treatment agent A and a treatment agent B. The treatment agent B comprises the following raw materials by mass: 30-40 parts of polyacrylamide, 8-10 parts of modified attapulgite clay, 5-6 parts of a compound, and 40-55 parts of deionized water. The modified attapulgite clay is strongly combined with protective films at oil-water and oil-sludge interfaces in the oil sludge through electrostatic force, hydrogen bonds and hydrophobic interaction, the protective films at the oil-water and oil-sludge interfaces are destroyed, oil drops are released and coalesced to form an oil phase, and separation of oil, water and sludge is achieved; positive charges carried in the modified attapulgite clay can be adsorbed to the surfaces of sludge particles with negative charges to be settled and separated out; carboxyl groups contained in the modified hemicellulose can be combined with hydrophilic groups of sophorolipid to form stable mixed micelles, so that the situation that the sophorolipid is easily influenced by the environment to lose efficacy, and oil drops are regathered and precipitated is avoided.

The invention discloses a remediation agent for high-concentration hexavalent chromium polluted soil and a preparation method and a remediation method thereof, and belongs to the technical field of soil pollution remediation. The remediation agent for the high-concentration hexavalent chromium contaminated soil comprises an acidic precursor, an iron-sulfur reducing agent and a synergist, the acidic precursor comprises organic acid and propylene glycol alginate; the iron-sulfur reducing agent comprises an iron-based reducing agent and a sulfur-based reducing agent; the synergist is prepared from sophorolipid and alkyl glucoside. The remediation agent can be used for high-concentration hexavalent chromium contaminated soil, hexavalent chromium in the soil is exposed in a Cr < 6 + > ion form as much as possible by replacing the hexavalent chromium in the soil, then the hexavalent chromium is converted into trivalent chromium by using a reducing agent, and the removal efficiency of the hexavalent chromium reaches up to 99.6%. In addition, the soil remediation agent is simple in use mode, rapid and thorough in reaction and lasting in effect.

The invention discloses a bumblebee candida for efficiently producing sophorolipid as well as a construction method and application of the bumblebee candida. According to the invention, metabolic transformation is carried out on a wild type strain of candida bumblebee, a peroxisome membrane transporter gene PXA1 is knocked out, so that a beta-oxidation pathway of fatty acid competing with a sophorolipid synthesis pathway is moderately inhibited, and on the basis, an endogenous potent promoter pENO is utilized to enhance expression of CYP52M1; therefore, the bumblebee candida engineering bacteria for efficiently producing sophorolipid are constructed. Compared with an original strain, the recombinant candida bumblebee has the advantages that efficient conversion of a fermentation substrate and high yield of sophorolipid can be realized, and the sophorolipid yield of shake flask fermentation reaches 88.50 g / L. The strain is fermented in a 5L fermentation tank and fermented for 7 days after rapeseed oil is added, 232.27 g / L of crude sophorolipid is produced, and high productivity is kept.

The invention relates to a quaternary ammonium salt type bio-based surfactant as well as a preparation method and application thereof, and the quaternary ammonium salt type bio-based surfactant is prepared by taking industrial sophorolipid as a raw material through ring-opening reaction and quaternization reaction. Compared with unmodified sophorolipid, the quaternary ammonium salt type bio-based surfactant disclosed by the invention has more excellent surface activity, lower contact angle and higher foaming capacity and wettability, the wetting reversal capacity on the oil phase surface is obviously improved, the viscosity reduction rate of heavy oil is higher than 99%, and the quaternary ammonium salt type bio-based surfactant has a better industrial application prospect in emulsification and viscosity reduction exploitation of heavy oil.

The invention provides a preparation method of a hydrolyzed collagen product with surface amphipathicity as well as the product and application thereof, and the preparation method comprises the following steps: (1) taking hydrolyzed collagen enzymatic hydrolysate as a substrate, then preparing a fermentation culture medium with vegetable oil and sugar, and fermenting by adopting candida bumblebee; (2) after fermentation, adding lysozyme into the fermentation liquor for activating treatment, and then sterilizing, centrifuging and filtering; and (3) carrying out freeze-drying treatment on the filtered fermentation liquor to obtain the hydrolyzed collagen product with surface amphipathicity. The hydrolyzed collagen product prepared by the invention not only has low molecular weight and high permeability to skin, but also has water locking and moistening effects on the skin surface due to the amphiphilic sophorolipid on the surface, so that the moisture retention of the skin is better improved, and the product has excellent product characteristics and has wide market prospects.

The invention relates to the technical field of cleaning agents, and particularly discloses a composite cleaning agent as well as a preparation method and application thereof. The composite cleaning agent comprises the following raw material components in percentage by mass: 5%-10% of inorganic particles, 5%-15% of a surfactant, 15%-30% of a cleaning aid and the balance of water, wherein the surface active agent is prepared from rhamnolipid, fatty acid methyl ester ethoxylate, cocamidopropyl betaine and sophorolipid; the inorganic particles comprise soluble sodium salt and nano silicon dioxide; the cleaning auxiliary agent comprises a geranium extracting solution, a herba portulacae extracting solution, lauric acid and glycerol. The composite cleaning agent is simple in raw material components, simple and convenient in preparation method, low in cost and free of secondary pollution to the environment. The composite cleaning agent effectively solves the problems that an existing train cleaning agent is poor in cleaning effect, complex in manufacturing process and unfriendly to the environment.

The invention belongs to the technical field of cosmetics, and particularly relates to a cosmetic delivery system based on a natural wall material, a preparation method and application. The cosmetic delivery system is of a core-shell structure, a core is a cosmetic active component, and a shell layer is a composite polymer gel network of sodium alginate, sophorolipid and cellulose. The natural polysaccharide composite wall material is green and safe, has good biocompatibility and strong compatibility with cosmetic formulas, and does not irritate the skin. The delivery system can effectively protect cosmetic active ingredients from being damaged by illumination and oxygen, and the storage stability of the active ingredients is remarkably improved; meanwhile, slow release and targeted transdermal absorption of active ingredients are realized, and the bioavailability is improved.

The invention provides a microbial composite remediation liquid organic fertilizer and a preparation method thereof, and aims to solve the problems of single function, limited pollution abatement capability and the like of a traditional liquid fertilizer. Through synergism of strains of bacillus velezensis, bacillus methylotrophicus, pseudomonas putida and methanogenic archaea, recycling of agricultural wastes such as straw and mushroom dreg compost and decomposition, extraction of humic acid through enzymolysis and a formula of green additives (mineral source potassium fulvate, sophorolipid and the like), chemical passivation of heavy metals, pesticide degradation and soil improvement are realized; and dynamic fermentation control and membrane separation processes are combined, so that the method has the characteristics of efficient repair and low-carbon sustainability, and an integrated solution is provided for pollution treatment of complex farmlands.

The present application relates to the technical field of genetic engineering, and in particular to a high-yield sophorolipid gene knockout engineering bacterium, a construction method thereof and an application, the engineering bacterium taking Bombus bornensis Candida as a starting strain, and using a gF-Hph-gR nucleotide sequence to replace a gene sequence seq2 in a sophorolipid synthesis path for regulating a lactonization reaction ， The gF-Hph-gR nucleotide sequence is SEQ ID No. 1, and the seq2 gene sequence is SEQ ID No. 2. The method provided by the present application uses fusion PCR to construct a seq2 gene knockout box with a resistance screening marker, and the shortest time required is 1 day, which provides a new way for efficiently constructing a sophorolipid production engineering strain. The high-yield sophorolipid gene knockout strain provided by the present application lays a foundation for large-scale industrial production of sophorolipid and meets market demand, and has a wide application prospect, economic value and social benefit.

The invention relates to a composition with effects of controlling oil and removing dandruff and application of the composition. The composition with the effects of controlling oil and removing dandruff comprises sophorolipid, myristicamidopropyl PG-dimethyl ammonium chloride phosphate, creatine, a phellinus linteus extract, a hop extract and sodium chloride. According to the composition, the sophorolipid, the myristicamidopropyl PG-dimethyl ammonium chloride phosphate, the creatine, the phellinus linteus extract, the humulus lupulus extract and the sodium chloride are matched for use, the synergistic interaction effect is achieved, the hair cleaning effect can be achieved, meanwhile, healthy water-oil balance is provided for scalp, and the hair care effect is achieved. The completeness of the scalp barrier is favorably kept, so that the sebum secretion of the scalp is better reduced, and the formation of dandruff is reduced.

The invention provides a drug-loaded sophorolipid microsphere, a preparation method thereof and application of the drug-loaded sophorolipid microsphere in preparation of a bionic cartilage scaffold. The preparation method of the drug-loaded sophorolipid microspheres comprises the following steps: (1) preparing a drug-containing sophorolipid solution; (2) preparing multiple emulsion; and (3) adding a horse radish peroxidase solution into the multiple emulsion for reaction to obtain the drug-loaded sophorolipid microspheres. The bionic cartilage scaffold sequentially comprises a bone layer, a transition layer and a cartilage layer from bottom to top, the bone layer is a cross-linked compound of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the transition layer is a physical mixture of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the cartilage layer is drug-loaded sophorolipid microspheres. The drug-loaded sophorolipid microspheres disclosed by the invention have good biocompatibility, excellent biocompatibility and good mechanical properties. The bionic cartilage scaffold utilizes the characteristics of the nHA and the drug-loaded sophorolipid microspheres to form a unique network structure, so that material failure caused by mechanical mismatching is avoided.

The invention relates to an additive for improving refrigeration efficiency of liquid water and a production process thereof, and belongs to the technical field of water additives. The additive comprises 70-80 parts by weight of a carrier, 3-6 parts by weight of modified sophorolipid, 1-2 parts by weight of sodium polyacrylate and 0.5-1.5 parts by weight of sodium molybdate, the carrier is of a core-shell structure, the core of the carrier is nano-copper, and the shell of the carrier is hydrophilic mesoporous silica doped with carboxylated carbon nanotubes; according to the additive prepared by the invention, the refrigeration efficiency of water is improved through the interaction among the carrier, the surfactant and liquid water.

The present invention relates to the use of rhamnolipids and / or sophorolipids in coating compositions for improving the wetting rate of powdery formulation components in such compositions, which results in a reduction of dust nuisance during the addition of the powdery formulation components and thus in an increase in occupational safety during the production of the coating compositions, and in obtaining an improved uniform color appearance of the dry paint film with respect to color tone differences, which are determined by ΔE and visual evaluation.

The present invention relates to a method for separating acidic sophorolipids, or lactone sophorolipids, in particular acidic sophorolipids rich in acetylated acid sophorolipids, from products containing lactone / acid sophorolipids, and to the acidic sophorolipids, in particular acidic sophorolipids rich in acetylated acid sophorolipids, obtained by said method.

This invention relates to the field of semiconductor cleaning technology and discloses a cleaning agent for semiconductor silicon wafers and its preparation method. This cleaning agent aims to solve the problem that traditional strong alkaline or phosphate-based wet electronic chemical cleaning agents easily corrode the silicon wafer surface, damage the crystal lattice, and thus affect electrical performance. This cleaning agent utilizes a multi-component synergistic effect: a surfactant system composed of alkyl glycosides and sophorolipids enhances particle wetting and stripping; lipase and silicon-pharmacoprotein precisely catalyze the removal of specific contaminants; sodium gluconate complexes metal ions; gelatin-polyacrylamide copolymer disperses contaminants to prevent secondary contamination; glycerol and trehalose maintain enzyme activity; and ultrapure water ensures the system meets the electronic-grade purity standards for wet electronic chemicals. Overall, it avoids strong alkaline corrosion, maintains the integrity of the silicon wafer lattice, and improves particle removal efficiency, meeting the high standards required for wet electronic chemical-based silicon wafer cleaning agents in advanced processes.

The invention belongs to the field of microbial fermentation and the technical field of daily chemicals, and particularly relates to deacetylated sophorolipid as well as a preparation method and application thereof. By using cheap culture medium components and screening out saccharomycetes with high yield of lactone type sophorolipid, the problem that the production cost of the sophorolipid is too high is effectively solved, and the application of the sophorolipid in various fields is promoted; through the step of carrying out enzymolysis on a fermentation substrate in advance, the problems of long single fermentation period, low thallus utilization rate, high energy consumption and high cost of traditional saccharomycetes are solved. A multi-round fermentation circulation system is designed, and bacteria liquid is settled and recycled and then inoculated and fermented, so that thalli are efficiently and repeatedly utilized, the unit yield is increased, the fermentation period is shortened, and the production cost is effectively controlled. The structure is adjusted through a resin modification technology, a final product mainly comprises acetylated sophorolipid, and compared with acetylated sophorolipid and deacetylated sophorolipid, the sophorolipid has better foam stability and sensitive skin repairing effect and is suitable for the application fields of cosmetics, washing care and the like.

The invention belongs to the technical field of microbial fermentation engineering, and particularly relates to immobilized yeast cell microspheres, a preparation method of the immobilized yeast cell microspheres and a method for producing sophorolipid. The immobilized yeast cell microspheres are prepared from the following raw materials in parts by weight: 60 to 100 parts of saccharomycetes, 60 to 300 parts of organic alkoxy silane, 30 to 100 parts of polyvinyl alcohol, 30 to 100 parts of a pore-foaming agent and 400 to 4000 parts of a cross-linking agent, the immobilized yeast cell microspheres prepared by matching saccharomycetes, organic alkoxy silane, polyvinyl alcohol, a pore-foaming agent, a sulfate cross-linking agent and a phosphate cross-linking agent according to a specific ratio can obviously reduce the activity loss of immobilized strains, improve the mass transfer performance of an immobilized carrier, relieve product inhibition, improve the fermentation production efficiency of sophorolipid and reduce the production cost of the sophorolipid. The production cost is reduced.

Rhamnolipids and / or sophorolipids can be used in coating compositions for improvement of the speed of wetting of pulverulent formulation constituents in such compositions, which leads to a reduction in dust nuisance during incorporation thereof and to the increase in occupational safety in the production of coating compositions. Rhamnolipids and / or sophorolipids can also be used in coating compositions for obtaining improved homogeneous colour appearance of the dry paint film with regard to difference in hue, ascertained from the delta E and visual assessment.

The present invention relates to disposable premoistened cleaning wipes impregnated with a cleaning liquid containing a sophorolipid surfactant. This formulation offers enhanced antibacterial and cleaning effects while minimizing skin irritation and maintaining optimal skin hydration. When compared to wet wipes lacking a sophorolipid surfactant, these wipes demonstrate superior performance in terms of both efficacy and skin-friendly properties.