132 results about "Sophorolipid" patented technology

The invention discloses a method for preparing acid sophorolipid, and belongs to the technical field of biological fermentation. According to the method, candida is used as a fermentation strain, a composite carbon source culture medium containing a water-soluble carbon source and an oil-soluble carbon source is adopted, and the content of residual oil is monitored in real time in the fermentation process; when the residual oil content exceeds a threshold value of 5g / L, the emulsifying agent of 0.1 g / L-0. 5g / L is supplemented, and the stirring rotating speed is increased by 20-50rpm, so that the emulsification of an oil phase and the utilization of the thalli on the oil-soluble carbon source are promoted. By accurately controlling the residual oil content in the fermentation process, the proportion of the acidic sophorolipid and the carbon source conversion rate are remarkably increased, the proportion of the acidic sophorolipid is increased to 60% from 30% of a traditional process, the carbon source conversion rate is increased to 65% from 40%, the problems that the increasing means of the proportion of the acidic sophorolipid is single, and the carbon source is wasted are effectively solved, and the method is suitable for industrial production. The method has the advantages of simple process, low cost, environmental protection and the like.

The invention belongs to the technical field of cosmetics, and particularly relates to a cosmetic delivery system based on a natural wall material, a preparation method and application. The cosmetic delivery system is of a core-shell structure, a core is a cosmetic active component, and a shell layer is a composite polymer gel network of sodium alginate, sophorolipid and cellulose. The natural polysaccharide composite wall material is green and safe, has good biocompatibility and strong compatibility with cosmetic formulas, and does not irritate the skin. The delivery system can effectively protect cosmetic active ingredients from being damaged by illumination and oxygen, and the storage stability of the active ingredients is remarkably improved; meanwhile, slow release and targeted transdermal absorption of active ingredients are realized, and the bioavailability is improved.

The present application relates to the technical field of genetic engineering, and in particular to a high-yield sophorolipid gene knockout engineering bacterium, a construction method thereof and an application, the engineering bacterium taking Bombus bornensis Candida as a starting strain, and using a gF-Hph-gR nucleotide sequence to replace a gene sequence seq2 in a sophorolipid synthesis path for regulating a lactonization reaction ， The gF-Hph-gR nucleotide sequence is SEQ ID No. 1, and the seq2 gene sequence is SEQ ID No. 2. The method provided by the present application uses fusion PCR to construct a seq2 gene knockout box with a resistance screening marker, and the shortest time required is 1 day, which provides a new way for efficiently constructing a sophorolipid production engineering strain. The high-yield sophorolipid gene knockout strain provided by the present application lays a foundation for large-scale industrial production of sophorolipid and meets market demand, and has a wide application prospect, economic value and social benefit.

The invention provides a drug-loaded sophorolipid microsphere, a preparation method thereof and application of the drug-loaded sophorolipid microsphere in preparation of a bionic cartilage scaffold. The preparation method of the drug-loaded sophorolipid microspheres comprises the following steps: (1) preparing a drug-containing sophorolipid solution; (2) preparing multiple emulsion; and (3) adding a horse radish peroxidase solution into the multiple emulsion for reaction to obtain the drug-loaded sophorolipid microspheres. The bionic cartilage scaffold sequentially comprises a bone layer, a transition layer and a cartilage layer from bottom to top, the bone layer is a cross-linked compound of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the transition layer is a physical mixture of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the cartilage layer is drug-loaded sophorolipid microspheres. The drug-loaded sophorolipid microspheres disclosed by the invention have good biocompatibility, excellent biocompatibility and good mechanical properties. The bionic cartilage scaffold utilizes the characteristics of the nHA and the drug-loaded sophorolipid microspheres to form a unique network structure, so that material failure caused by mechanical mismatching is avoided.

The invention relates to an additive for improving refrigeration efficiency of liquid water and a production process thereof, and belongs to the technical field of water additives. The additive comprises 70-80 parts by weight of a carrier, 3-6 parts by weight of modified sophorolipid, 1-2 parts by weight of sodium polyacrylate and 0.5-1.5 parts by weight of sodium molybdate, the carrier is of a core-shell structure, the core of the carrier is nano-copper, and the shell of the carrier is hydrophilic mesoporous silica doped with carboxylated carbon nanotubes; according to the additive prepared by the invention, the refrigeration efficiency of water is improved through the interaction among the carrier, the surfactant and liquid water.

This invention relates to the field of semiconductor cleaning technology and discloses a cleaning agent for semiconductor silicon wafers and its preparation method. This cleaning agent aims to solve the problem that traditional strong alkaline or phosphate-based wet electronic chemical cleaning agents easily corrode the silicon wafer surface, damage the crystal lattice, and thus affect electrical performance. This cleaning agent utilizes a multi-component synergistic effect: a surfactant system composed of alkyl glycosides and sophorolipids enhances particle wetting and stripping; lipase and silicon-pharmacoprotein precisely catalyze the removal of specific contaminants; sodium gluconate complexes metal ions; gelatin-polyacrylamide copolymer disperses contaminants to prevent secondary contamination; glycerol and trehalose maintain enzyme activity; and ultrapure water ensures the system meets the electronic-grade purity standards for wet electronic chemicals. Overall, it avoids strong alkaline corrosion, maintains the integrity of the silicon wafer lattice, and improves particle removal efficiency, meeting the high standards required for wet electronic chemical-based silicon wafer cleaning agents in advanced processes.

The invention belongs to the field of microbial fermentation and the technical field of daily chemicals, and particularly relates to deacetylated sophorolipid as well as a preparation method and application thereof. By using cheap culture medium components and screening out saccharomycetes with high yield of lactone type sophorolipid, the problem that the production cost of the sophorolipid is too high is effectively solved, and the application of the sophorolipid in various fields is promoted; through the step of carrying out enzymolysis on a fermentation substrate in advance, the problems of long single fermentation period, low thallus utilization rate, high energy consumption and high cost of traditional saccharomycetes are solved. A multi-round fermentation circulation system is designed, and bacteria liquid is settled and recycled and then inoculated and fermented, so that thalli are efficiently and repeatedly utilized, the unit yield is increased, the fermentation period is shortened, and the production cost is effectively controlled. The structure is adjusted through a resin modification technology, a final product mainly comprises acetylated sophorolipid, and compared with acetylated sophorolipid and deacetylated sophorolipid, the sophorolipid has better foam stability and sensitive skin repairing effect and is suitable for the application fields of cosmetics, washing care and the like.

The invention belongs to the technical field of microbial fermentation engineering, and particularly relates to immobilized yeast cell microspheres, a preparation method of the immobilized yeast cell microspheres and a method for producing sophorolipid. The immobilized yeast cell microspheres are prepared from the following raw materials in parts by weight: 60 to 100 parts of saccharomycetes, 60 to 300 parts of organic alkoxy silane, 30 to 100 parts of polyvinyl alcohol, 30 to 100 parts of a pore-foaming agent and 400 to 4000 parts of a cross-linking agent, the immobilized yeast cell microspheres prepared by matching saccharomycetes, organic alkoxy silane, polyvinyl alcohol, a pore-foaming agent, a sulfate cross-linking agent and a phosphate cross-linking agent according to a specific ratio can obviously reduce the activity loss of immobilized strains, improve the mass transfer performance of an immobilized carrier, relieve product inhibition, improve the fermentation production efficiency of sophorolipid and reduce the production cost of the sophorolipid. The production cost is reduced.

The invention belongs to the technical field of fertilizers and relates to a compound fertilizer for black beans as well as a preparation method and application thereof. The compound fertilizer comprises a basic fertilizer, a microbial fertilizer and a trace element fertilizer, and a preparation method of the microbial fertilizer comprises the following steps: treating lignite with sophorolipid and trehalose lipid, treating turf with surfactin, mixing the treated lignite and turf, and then inoculating bacillus subtilis and rhizobium to obtain the microbial fertilizer. Through a unique dual mechanism of carrier synergism and fungicide function complementation, the dry matter cumulant and nitrogen cumulant of the overground part of the black bean are remarkably increased, and finally the yield and quality are remarkably improved.

The application provides a nanoemulsion and a preparation method and application thereof. The total amount of raw materials for preparing the nanoemulsion is 100%, and the raw materials include the following components: 1-40% of a composite surfactant, 1-25% of an oil phase, 1-30% of a co-surfactant, and the balance of a water phase; wherein the mass of the composite surfactant is 100%, and the composite surfactant includes the following components: 5-25% of a biological surfactant, 35-65% of a fatty alcohol polyoxyethylene ether surfactant, 1-20% of dodecyl benzene sulfonic acid and / or sodium salt thereof, 1-20% of oleic acid, and 1-5% of citric acid; the biological surfactant includes one or more than two combinations of sophorolipid, rhamnolipid, trehalose lipid, surfactin, alkyl polyglycoside, phosphatidylcholine, amino acid lipid, saponin, phospholipid, or derivatives thereof; and the average particle size of the emulsion droplets in the nanoemulsion is 1.5-5 nm. The nanoemulsion has excellent recovery performance.

The invention discloses a composition for skin cleaning and application thereof, and belongs to the field of daily chemicals. The composition comprises glycolipid and a lauryl alcohol polyether carboxylic acid surfactant in a specific weight ratio. Wherein the glycolipid is preferably sophorolipid, and the lauryl alcohol polyether carboxylic acid surfactant is preferably lauryl alcohol polyether-5 carboxylic acid. According to the composition disclosed by the invention, the cleaning efficiency on unsaturated fatty acids (such as oleic acid and squalene) on the skin surface is remarkably improved on the premise of not excessively adding a high-effect anionic surfactant or sacrificing the product mildness. Meanwhile, the composition can effectively reduce degeneration damage to skin keratoprotein, has good skin mildness and safety, and is suitable for washing-off type personal care products for face cleaning, hair washing, bathing and the like.

The invention discloses a litchi leaf surface adaptive aviation pesticide application auxiliary agent and a preparation method thereof. The active ingredients of the litchi leaf surface adaptive aviation pesticide application auxiliary agent are sophorolipid, sodium dodecyl benzene sulfonate and a C6 fluorocarbon surfactant, and the mass ratio of the sophorolipid to the sodium dodecyl benzene sulfonate to the C6 fluorocarbon surfactant is (7.0-7.5): (1.3-1.5): 1 in sequence. Experiments prove that when a plant protection unmanned aerial vehicle is adopted to spray litchis, the aerial spraying liquid can improve the surface tension of the liquid medicine, enhance the evaporation resistance, spreadability and wettability of fog drops, and improve the deposition distribution of the fog drops of the plant protection unmanned aerial vehicle on the canopy of a litchi tree.

The application belongs to the field of biological chemical industry, and particularly relates to a solvent-free green synthesis method of lactone sophorolipid based on enzymes. Acid-type sophorolipid is used as a substrate, dissolved in an organic monohydric alcohol, and a lipase is used as a catalyst to generate lactone sophorolipid. The application uses a solvent-free system, does not introduce other compounds, and greenly prepares lactone sophorolipid. The application has the advantages of safety, high efficiency, no pollution, no introduction of other chemical reagents, simple operation method, and simple product extraction.

The invention discloses an hph resistance gene knockout box based on gme5 gene editing, a genetically engineered bacterium and application of the hph resistance gene knockout box and the genetically engineered bacterium. The hph resistance gene knockout box comprises a recombinant DNA construction body with the nucleotide sequence shown as SEQ ID NO: 1, the recombinant DNA construction body replaces an endogenous regulatory factor gme5 gene through homologous recombination in a targeted mode, and the nucleotide sequence of the regulatory factor gme5 gene is shown as SEQ ID NO: 2. According to the application, the bumblebee candida albicans are directionally modified through a genetic engineering strategy, an engineering strain for synthesizing the high-yield lactone type sophorolipid is constructed, and the lactonase activity and fatty acid transport capacity are synergistically enhanced, so that the ratio of the lactone type sophorolipid is maximized, and the yield of the lactone type sophorolipid is increased. In the sophorolipid produced by using the genetically engineered bacterium and the fermentation production method disclosed by the invention, the total content of the sophorolipid is 358.77 g / L, and the lactone content accounts for 74% or more of the total sophorolipid, so that the separation and purification difficulty and the production cost are greatly reduced, and an efficient and low-cost solution is provided for industrial fermentation production of the sophorolipid.

The invention discloses a biocontrol bacterium agent and a preparation method and application thereof.The biocontrol bacterium agent comprises a nano carrier and biocontrol bacteria, the carrier is halloysite nanotubes modified by coating the surfaces of the halloysite nanotubes with lecithin, sucrose ester or sophorolipid, and the biocontrol bacteria are bacillus subtilis; the preparation method comprises the following steps: (1) soaking halloysite nanotubes in a hydrochloric acid solution for pretreatment; (2) adding halloysite, lecithin, sucrose ester or sophorolipid into a solvent, uniformly mixing, reacting, and after the reaction is completed, washing and drying to obtain a modified halloysite nanotube; and (3) mixing a bacillus subtilis liquid with the modified halloysite nanotube, incubating, and drying to obtain the halloysite nanotube. According to the invention, lecithin, sucrose ester or sophorolipid is adopted to modify the halloysite nanotube and then is used as a carrier, so that the loading effect on bacillus subtilis is improved, and the fungicide has excellent prevention and treatment effects on tobacco brown spot, black shank, anthracnose and sweet leaf fungus damping-off.

The invention relates to the technical field of biological medicine, and particularly discloses a targeted nano NK cell exosome gel loaded with lycopene and application thereof, the targeted nano NK cell exosome gel loaded with lycopene is prepared from the following components in percentage by mass: 0.1 to 1 weight percent of lycopene oleoresin, 0.5 to 5 weight percent of NK cell exosome, 60 to 80 weight percent of gel system and the balance of ultrapure water; the lycopene oleoresin is prepared by the following steps: firstly, adding a eutectic solvent into a tomato fermentation extracting solution, carrying out ultrasonic extraction on lycopene, and then uniformly mixing the lycopene, sophorolipid and vegetable oil. According to the application, the NK cell exosome and the lycopene are organically matched, the NK cell exosome directly acts on the local part of the cervix uteri through the NK cell, and meanwhile, the lycopene can repair the damaged cervix uteri cell and restore the damaged cervix uteri cell to a normal structure, so that the purposes of removing viruses and preventing and treating cervical cancer are achieved.

The invention discloses a medical sterilizing, antagonistic-free and fat-retaining disinfectant hand sanitizer and a preparation method thereof, and relates to the technical field of hand sanitizers. CHG is used as a main bactericide, sophorolipid, CAB and alpha-glucan oligosaccharide are used as a composite surfactant, and the antagonistic effect of sterilization effect reduction caused by CHG wrapping after a conventional surfactant forms micelles is eliminated; meanwhile, phytosterol ester, glycosylglycerol and madecassoside are introduced as skin fat-retaining components, so that the sebum retention rate is greater than or equal to 95%, meanwhile, the antibacterial rate on staphylococcus aureus is still kept 100%, and the hand washing liquid is suitable for high-frequency hand washing of medical staff.

The invention relates to the technical field of oilfield chemistry, in particular to an environment-friendly biological blocking remover for oil extraction and a processing technology of the environment-friendly biological blocking remover. The environment-friendly biological blocking remover is prepared from the following components in percentage by weight: 4 to 6 percent of alkyl glycoside, 4 to 6 percent of sophorolipid, 3 to 5 percent of methylglycine diacetic acid, 5 to 8 percent of rhamnolipid, 6 to 8 percent of compound microorganism fermentation liquor, 5 to 8 percent of lipase, 5 to 8 percent of protease, 6 to 8 percent of polymer degrading enzyme, 2 to 4 percent of citric acid, 2 to 4 percent of mandelic acid, 1 to 2 percent of glycerol, 1 to 2 percent of trehalose and 31 to 57 percent of deionized water. The biological enzyme, the biological surfactant, the organic acid and the chelating agent cooperate to fundamentally disintegrate blockages, meanwhile, the microcapsule technology and the nano-bubble technology are used, the action period is greatly prolonged, blockages of deeper parts can be treated, and the treatment effect is good. And corrosion and damage of strong acid and strong alkali to underground pipe columns, tools and stratum rocks are avoided, and secondary damage caused by plug removal operation is prevented.

The application discloses a surfactant composition, a surfactant system and application thereof, and belongs to the technical field of cosmetics, and comprises the following components in parts by weight: 5-11 parts of sodium C14-16 olefin sulfonate, 1-6 parts of an amino acid surfactant, 0.5-5 parts of an alkyl polyglycoside surfactant, 0.1-2 parts of a bio-based surfactant and 1-5 parts of an amphoteric surfactant; the bio-based surfactant comprises at least one of sophorolipid, rhamnolipid, soapberry extract and oil tea dregs extract. The composition disclosed by the application has very low surface tension and cmc value, the molecules in a surface adsorption layer are closely arranged to promote surface adsorption, the foaming performance and foaming stability are effectively improved, good stability is achieved in a wide pH (5-10) range, excellent high and low temperature stability is achieved, the surfactant composition disclosed by the application has low irritation, is safe to use, and has a wide application prospect.

To increase occupational safety and provide improved homogeneous colour appearance of a dry coating film in the production of a coating composition.SOLUTION: The present invention discloses the use of rhamnolipids and / or sophorolipids in coating compositions, for improved speed of wetting of pulverulent formulation constituents in the coating compositions, which leads to a reduction in dust nuisance during incorporation of the pulverulent formulation constituents, resulting in increased occupational safety in the production of the coating compositions, providing the improved homogeneous colour appearance of a dry coating film with regard to difference in hue, ascertained from the ΔE and visual assessment.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of skin care products, and relates to an L-carnosine-sophorolipid micelle and a preparation method and application thereof.The method comprises the steps that sophorolipid is dissolved in methyl alcohol or ethyl alcohol or acetone, L-carnosine is dissolved in an aqueous solution with the pH ranging from 2 to 7, after mixing and stirring are conducted according to the mass ratio of 3: 1-8: 1, vacuum rotary evaporation is conducted under the pressure ranging from-0.06 MPa to-0.08 MPa at the temperature ranging from 30 DEG C to 70 DEG C, an organic solvent is removed, a thin film is formed, and the L-carnosine-sophorolipid micelle is obtained. And hydrating with normal saline, purifying with a 1000Da ultrafiltration membrane, and freeze-drying to obtain the product. The obtained micelles are uniform in particle size, the encapsulation efficiency is up to 99.34%, the carnosinase resistance and the transdermal absorption efficiency of the L-carnosine can be improved, and meanwhile, the effects of scavenging free radicals, resisting inflammation, inhibiting bacteria and preserving moisture for a long time are synergistically reserved. The process is simple, convenient and controllable, the problems that the L-carnosine is poor in stability and low in bioavailability are solved, and the preparation method is suitable for smearing type skin care products and has important application value.

A cleansing composition comprises one or more biosurfactants and one or more taurate surfactants. The biosurfactants may be selected from a glycolipid e.g. sophorolipids, a lipopeptide or a phospholi

The utility model provides a sophorolipid sterile fermentation device which comprises a tank body, a jacket, a steam inlet pipe, a steam outlet pipe, a cooling water inlet pipe, a cooling water outlet pipe, a liquid discharge pipe, a gas inlet pipe and a detection control system, and the detection control system is used for collecting and adjusting internal environment data of the tank body. The sophorolipid fermentation device has a plurality of temperature control modes, is convenient in temperature regulation, adopts the double temperature control modes of the electric heating pipe and steam heating, and is combined with a cooling water cooling system, so that the temperature regulation is more flexible, the accurate control of the temperature in the fermentation process is improved, and the optimal fermentation environment of sophorolipid is favorably maintained. The liquid level sensor, the pressure sensor, the temperature sensor, the pH sensor and the dissolved oxygen sensor are adopted, key parameters of fermentation liquor can be collected in real time, accurate monitoring of the fermentation environment is ensured, the fermentation efficiency and the product quality can be improved, and good economic benefits are achieved.

The present invention relates to a process of separating an acidic sophorolipid, or a lactonic sophorolipid, especially an acidic sophorolipid enriched with acetylated acidic sophorolipid from a lactonic / acidic sophorolipid-containing product and the acidic sophorolipid, especially the acid-ic sophorolipid enriched with acetylated acidic sophorolipid obtained by said process.

The invention discloses a method for preparing a granular biogas residue soil conditioner for both a feeding machine and a machine, which comprises 90-100 parts of biogas residue with the water content of 3-5% after dry-wet separation, 5-10 parts of an acid regulating agent, 3-5 parts of a binder, 20-30 parts of turf, 15-25 parts of perlite, 5-8 parts of mineral fertilizer, 2-4 parts of trace elements and 1-2 parts of sophorolipid with the concentration of 3% and used as a wetting agent. An acid regulating agent, a binding agent, trace elements and the like are scientifically proportioned, the acid regulating agent is formed by mixing vinegar residues and furfural residues according to a specific proportion according to different soil types and can be flexibly adjusted according to the soil acidity and alkalinity difference, and the binding agent is formed by mixing konjac polysaccharide and modified chitosan according to a certain proportion and is stable in binding performance in a humid environment; the forming effect of the modifier particles is remarkably improved, the modifier particles are not prone to being damped and broken in the storage process, the modifier particles can be evenly scattered in the field application process, it is guaranteed that the modifier makes full contact with soil, and therefore the soil improvement effect is effectively improved.

The invention belongs to the field of enzymes, and particularly relates to a CYP52M1 enzyme mutant and application of the CYP52M1 enzyme mutant in sophorolipid production. The CYP52M1 enzyme mutant is a CYP52M1 enzyme mutant which is obtained by mutating at least one site of amino acids at the 141 site, the 148 site and the 476 site of an amino acid sequence as shown in SEQ ID NO.1, and the CYP52M1 enzyme mutant is a CYP52M1 enzyme mutant, according to the mutation, phenylalanine at the 141 site of the amino acid sequence shown in SEQ ID NO.1 is mutated into alanine, tryptophan at the 148 site is mutated into alanine, or proline at the 476 site is mutated into alanine. The mutant enhances the substrate binding orientation and omega-hydroxylation ability, improves the fatty acid conversion efficiency, reduces the cost, and promotes the efficient synthesis of sophorolipid. The invention provides a new technical approach for improving the yield of the biosurfactant through an enzyme engineering means.

A composition for a anticaking coating additive, which comprises: derivatized carbohydrates like alkyl derivatized polyglycosides, glycolipids, glycerol glycolipids, sphingo glycolipids, sulfolipids, phospholipids, glucosides, rhamnolipids, sophorolipids, and others.

The invention provides a high systemic pyriproxyfen composition and a preparation method and application thereof.The composition is prepared from, by weight, 1-20% of pyriproxyfen serving as an active ingredient, 10-50% of rosin-based vegetable oil serving as a synergistic additive and 0-20% of sophorolipid. The permeability of the pyriproxyfen on leaf surfaces and the conductivity of the pyriproxyfen in plants are changed, the conduction of the pyriproxyfen from a pesticide application part to a newborn tissue and a target pest feeding part is promoted, and the quick-acting property, the persistence and the pesticide effect of the preparation are improved.

The application belongs to the field of chemical materials, and particularly relates to a composite material with high wetting and application thereof, and more particularly relates to a composite material containing a surfactant, the composite material comprising the following components: component A and component B; wherein the component A is a surfactant, preferably a biosurfactant, and is specifically selected from sophorolipid, sodium lauroyl glutamate, sodium cocoyl glycinate, NP-7, NP-9, NP-10, NSS or Triton X100; and the component B is selected from limonene, sweet orange essential oil, dried tangerine peel essential oil, pine essential oil or limonene. Through wetting force performance testing on the composite material, under the special properties of the sophorolipid surfactant and through the joint action with reagents, obvious wetting force improvement can be detected.