Method for producing sophorose ester through fermenting waste molasses and waste glycerin

A technology of waste glycerin and waste molasses, which is applied in the field of fermentation to produce sophorolipids, can solve the problems of unfavorable promotion and application of sophorolipids, large loss rate of extraction process, low yield of sophorolipids, etc., and achieve simple and easy fermentation process, Obvious advantages in the market and the effect of solving large emission pollution

Inactive Publication Date: 2012-01-25
天津实发中科百奥工业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the method of fermenting vegetable oil to produce sophorolipids is usually used. Due to the higher price of vegetable oil, longer fermentation period, more complicated production process, and the greater loss rate of the post-extraction process, the yield of sophorolipids is also low. The cost of sophorolipids is higher, and the market price is also higher, which is not conducive to the popularization and application of sophorolipids.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Preparation of spore suspension: Inoculate Torulopsis globosa TJZKBA10326 on the slant of the culture medium, culture at a constant temperature of 30°C for 2 days, add 0.75% normal saline to wash the mycelium, obtain the bacterial suspension, and suspend The spore content in the liquid is about 3.6×10 5 One, ready for use; slant medium composition includes (1L): glucose 8g, malt powder 3g, peptone 4g, yeast powder 4g, agar 15g, dilute to 1L with distilled water, pH 3.5. The strain used is: Torulopsis bombicola TJZKBA10326.

[0017] (2) The above bacterial suspension is used to inoculate into the sterilized liquid seed medium. The composition of the liquid seed medium includes (1L): 30g glucose, 30g corn oil, 5g yeast powder, 0.5g urea, distilled water to volume to 1 L, pH 3.5. The liquid seed culture was sterilized at 121°C for 15 minutes and then cooled; then the spore suspension was added to the above-mentioned cooled liquid seed medium according to the inoculat...

Embodiment 2

[0021] (1) Preparation of spore suspension: Inoculate Torulopsis globosa TJZKBA10326 on the slope, culture at 30°C for 2 days, add 0.75% normal saline to wash the mycelia, and obtain the bacterial suspension. The spore content is about 4.5×10 5 One, ready for use; slant medium composition includes (1L): glucose 8g, malt powder 3g, peptone 4g, yeast powder 4g, agar 15g, dilute to 1L with distilled water, pH 3.5.

[0022] (2) The above bacterial suspension is used to inoculate into the sterilized liquid seed medium. The composition of the liquid seed medium includes (1L): 50g of glucose, 50g of corn oil, 7g of yeast powder, 0.8g of urea, and distilled water to volume to 1 L, pH 3.5. The liquid seed culture was sterilized at 121°C for 15 minutes and then cooled; then the spore suspension was added to the above-mentioned cooled liquid seed medium according to the inoculum volume of 4% by volume (the liquid volume of the 500ml shake flask is 100ml), shake The bottle was cultivate...

Embodiment 3

[0027] (1) Preparation of spore suspension: inoculate Torulopsis globularis TJZKBA10326 on the slope, culture at 30°C for 3 days, add 0.75% normal saline to wash the mycelia, and obtain the bacterial suspension. The spore content is about 5.8×10 5 One, ready for use; slant medium composition includes (1L): glucose 8g, malt powder 3g, peptone 4g, yeast powder 4g, agar 15g, dilute to 1L with distilled water, pH 3.5.

[0028] (2) The above bacterial suspension is used to inoculate into the sterilized liquid seed medium. The composition of the liquid seed medium includes (1L): 60g of glucose, 60g of corn oil, 8g of yeast powder, 1g of urea, distilled to volume with distilled water 1L, pH 3.5. The liquid seed culture was sterilized at 121°C for 15 minutes and then cooled; then the spore suspension was added to the above-mentioned cooled liquid seed medium according to the inoculum volume of 3% by volume (the liquid volume of the 500ml shake flask is 100ml), shake The bottle was i...

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PUM

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Abstract

The invention relates to a method for producing sophorose ester through fermenting waste molasses and waste glycerin, which comprises the following implementation steps that: (1) spore suspension preparation: torulopsis utilis TJZKBA 10326 is inoculated on the inclined surface of a culture medium and is cultured for 2 to 4 days at the constant temperature being 30 DEG C, and normal saline with the mass concentration being 0.75 percent is added for washing mycelia, and the bacterial suspension is obtained; (2) the bacterial suspension is used for being inoculated into the sterilized liquid seed culture medium, the spore suspension is inoculated into the cooled liquid seed culture medium according to the inoculation quantity with the volume percentage being 2 to 5, and liquid seeds are obtained; (3) the liquid seed culture medium is used for being inoculated into a sterilized 5L fermentation tank, then, the liquid culture medium is inoculated into the cooled fermentation tank culture medium according to the inoculation quantity with the volume percentage being 2 to 5, the fermentation temperature is 30 to 34 DEG C, the dissolved oxygen is controlled to be 30 to 40 percent, and the fermentation time is 48 to 72h; and (4) after the fermentation is completed, the mixed organic solvent of organic solvent ethyl acetate / isopropanol with the same volume as the fermentation liquid is used for extracting the sophorose ester. The method has the advantages that the process is simple, the cost is low, and the effect is obvious.

Description

technical field [0001] The invention relates to a method for fermenting and producing sophorolipids, in particular to a method for fermenting and producing sophorolipids with waste molasses and waste glycerol. Background technique [0002] Soybean molasses is a by-product obtained during the production of concentrated soybean protein. Soybean molasses is rich in soybean oligosaccharides, which can be used as raw materials for the production of functional soybean oligosaccharides. Wherein the main component content is: total sugar 54.64% (among them sucrose 32.17%, raffinose 4.27%, stachyose 17.54%), total lipid 18.00%, total steroid glycoside 3.06%, total phospholipid 8.79%, crude protein 8.71%, Ash content 8.69%. [0003] Glycerin, also known as glycerol, is a colorless, odorless, sweet, viscous liquid. The chemical structure of glycerol is completely different from carbohydrates, so it does not belong to the same class of substances. Waste glycerin has a wide range of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12N1/16C12R1/645
Inventor 徐勇虎李睿颖国华王永乐刘云胡娅君王晓琼范婷韩慧墨玉欣刘晓鸥孙溪
Owner 天津实发中科百奥工业生物技术有限公司
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