32 results about "Sophorose" patented technology

The present invention is directed to the use of rebaudioside A or rebaudioside D in combination with one or more compounds of Formula (I), or a stereoisomer thereof, wherein R2 is rhamnose, and R and R1 are each independently selected from the group consisting of hydrogen, glucose, and beta-sophorose, for enhancing the sweet taste of carbohydrate sweeteners, such as sucrose and fructose. The present invention is also directed to consumables which include a combination of a carbohydrate sweetener, rebaudioside A or rebaudioside D, and one or more compounds of Formula (I), or a stereoisomer thereof.

The invention relates to a method for producing sophorose ester through fermenting waste molasses and waste glycerin, which comprises the following implementation steps that: (1) spore suspension preparation: torulopsis utilis TJZKBA 10326 is inoculated on the inclined surface of a culture medium and is cultured for 2 to 4 days at the constant temperature being 30 DEG C, and normal saline with the mass concentration being 0.75 percent is added for washing mycelia, and the bacterial suspension is obtained; (2) the bacterial suspension is used for being inoculated into the sterilized liquid seed culture medium, the spore suspension is inoculated into the cooled liquid seed culture medium according to the inoculation quantity with the volume percentage being 2 to 5, and liquid seeds are obtained; (3) the liquid seed culture medium is used for being inoculated into a sterilized 5L fermentation tank, then, the liquid culture medium is inoculated into the cooled fermentation tank culture medium according to the inoculation quantity with the volume percentage being 2 to 5, the fermentation temperature is 30 to 34 DEG C, the dissolved oxygen is controlled to be 30 to 40 percent, and the fermentation time is 48 to 72h; and (4) after the fermentation is completed, the mixed organic solvent of organic solvent ethyl acetate / isopropanol with the same volume as the fermentation liquid is used for extracting the sophorose ester. The method has the advantages that the process is simple, the cost is low, and the effect is obvious.

The present invention is directed to the use of a compound of Formula I,or a stereoisomer thereof, wherein R2 is rhamnose, and R and R1 are each independently selected from the group consisting of hydrogen, glucose, and beta-sophorose, for enhancing the sweet taste of carbohydrate sweeteners, such as sucrose and fructose. The present invention is also directed to consumables comprising a combination of a carbohydrate sweetener and a compound of Formula I, or a stereoisomer thereof.

This invention discloses a quasi-Wicker yeast variety Wickerhamiella domercqiae var. sophorolipid. This strain is conserved in general microbe center in China microbe bacterial conservention and management committee (institute of microbe of Chinese Academy of Sciences, Beijing, China), and its preservation number is CGMCC No. 1576. This invention also discloses the appliance of the quasi-Wicker yeast variety in preparing sophorosid biosurfactant that can supress neoplasms outside of body. The sophorosid is prepared through following steps: (1) chamfer culture(2)seed culture(3) enlargement culture(4) fermentate collection(5)production separation, and others. This method has many advantanges, for example, it is easy to operate; the output of sophorosid is high; the generated sophorosid has high restraining rate to tumor cell...so it has very broad application prospect in drug field.

The invention provides a fermentation method for producing high-activity acid cellulase from trichoderma reesei, which comprises the following steps: S1, inoculating a trichoderma reesei strain into a culture medium, separating to obtain a single colony, culturing seeds, and preserving; s2, inoculating the preserved trichoderma reesei into an activated culture medium for culturing; S3, inoculating the culture solution in the step S2 into a seed amplification culture medium for culturing; and S4, inoculating the culture solution in S3 into a fermentation culture medium, and carrying out liquid submerged fermentation to obtain fermentation liquor containing acidic cellulase. According to the method, culture medium components and fermentation conditions are optimized, sophorose is used for inducing enzyme production, trichoderma reesei cellulase production is divided into two stages, the feeding speed in the enrichment period is increased as much as possible so that thalli are rapidly proliferated to 30% of wet weight, after the thalli density is reached, the feeding speed is reduced, the thalli enter the induced enzyme production period, glucose is rapidly consumed, the low-concentration sophorose is transferred into cells to play a strong induction role, so that the high-activity acidic cellulase is obtained in fermentation liquor.

The purpose of the present invention is to provide the following: a dried influenza vaccine preparation with which stable retention of the activity of influenza vaccine antigen is possible, even when the preparation is stored without strict low-temperature management, and with which a stable supply is possible; and a method for producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation comprising influenza vaccine antigen and a disaccharide, wherein the disaccharide is one or more selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

The present invention concerns a method for producing sophorose from a sophorolipid which involves carrying out a step of hydrolysis of the sophorolipid in an aqueous medium in the presence of an acid compound.

The invention relates to a beta-glucosidase and an application thereof, wherein the beta-glucosidase comprises: a, enzyme with the sequence of SEQ ID NO:1; and b, enzyme which is derived from the enzyme in step a through substituting, deleting or adding one or a plurality of amino acids and has the activity of the enzyme in the step a. The selected beta-glucosidase provided by the invention has the deoxyglucose and hygromycin resistance and transglycosylation, under the transglycosylation, cellobiose and other oligosaccharides can generate sophorose and other inductors, and enter cells through a constitution permease system on a cell membrane, and the synthesis of cellulose in the cells is initiated. Consequently, the beta-glucosidase can be used for the production of catalytically synthesizing the sophorose to induce the cellulose, and thereby having a greater commercial value.

The invention discloses a method for high-yielding cellulose through trichoderma fungi. The irradiation technology is introduced to modify rice straw, the inducing effect is improved, and the segmentation inducing fermentation technology is adopted. The method specifically comprises the following steps that under the aseptic environment, spores of the trichoderma fungi are inoculated to a PDA plating medium to be cultured, a circle of spores are picked to be inoculated to a seed medium, and seed liquid is obtained after shaking table shake culturing is conducted; the obtained seed liquid is inoculated to a fermentation enzyme producing culture medium containing lactose to be subjected to fermentation cultivation according to a certain inoculation amount; a fresh carbon source containing sophorose is added to a fermentation system through aseptic operation, and fermentation cultivation continues to be conducted; after fermentation is finished, the obtained fermentation liquid is subjected to centrifugal separation, and obtained supernate is the high-yielding cellulose enzyme liquid. A cellulose enzyme system obtained through the method is more perfect in structure and high in yield.

The present invention relates to the field of microbial production of novel biosurfactants. More specifically, the present invention discloses the usage of a fungal strain such as the yeast Starmerellabombicola having a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase for producing high amounts of so-called “symmetrical bolaform sophorosides” where both sophorose moieties are attached through a terminal glycosidic linkage to the hydrophobic linker. In addition, the present invention further discloses that the latter yeast can also be used to produce alkyl sophorosides and symmetrical bolaform glucosides.

The present invention provides a method to produce RD by using a cutinase to catalyze the esterification of RB under stepwise cooling temperatures, which is related to the field of biosynthesis of organic compounds. The method uses a cutinase from Thermobifida fusca to catalyze the esterification of RB and sophorose to produce RD. The stepwise cooling temperatures are used to reduce the heat inactivation of the enzyme as well as to improve the mass transfer. The method catalyzes the esterification of RB to produce RD in a solvent such as methanol, DMSO and DMF. The reaction is safe, efficient and highly selective. In addition, the method uses stepwise additions of substrate RB and cooling temperatures for the esterification reaction. In this way, it speeds up the initial reaction rate, increases the amount of solved RB as it is converted to RD, and improves the mass transfer to further increase the reaction speed. In summary, the method uses moderate reaction conditions, and has a high yield and a simple purification procedure.

The invention discloses an application of sophorose in preparation of products for preventing and treating obesity. The invention further discloses corresponding products of the sophorose. According to the application and the corresponding products, the sophorose is used for lowering appetite and properly stimulating generation of short-chain fatty acids so as to achieve the effect of controllingbody weight; and the regulating and controlling action is relatively moderate and is relatively good in acting effect, and direct acting on a brain, acceptors or enzymes is avoided, so that compared with other drugs, the products do not have side effects, can be eaten as part of daily meals and can also be applied to drugs for preventing and treating obesity patients. The sophorose can also be used for weakening adverse effects such as fasting blood-glucose rising and impaired glucose tolerance dose resulting from high-fat meals.

The invention discloses a method for hydrolyzing stevioside with rich sources by using acid and synchronously preparing two products with high added values, namely isosteviol and sophorose. An isosteviol product is a medicine with functions of reducing blood sugar, regulating lipid, and the like, the sophorose is a most effective inducer for producing cellulase from strains such as trichoderma, and the like, and the two products have broad application prospect respectively. The method is characterized in that by controlling appropriate conditions, the two high added value products with important industrial application value and medical value, namely the isosteviol and the sophorose are synchronously prepared, and a one-step hydrolysis method is adopted.