Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for pre-treating sophorolipid fermentation solution and separating and purifying sophorolipid with different structures

A technology for separation and purification of sophorolipids, which is applied in the field of preparation and purification of biosurfactant sophorolipids, can solve the problem of low organic solvent usage, achieve reduced usage, huge economic value, and improve safety and environmental protection sexual effect

Active Publication Date: 2019-04-26
HEFEI UNIV OF TECH
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the deficiencies in the current large-scale industrial production of sophorolipids in the late stage of fermentation liquid treatment and extraction of different products, and provide a method for the pretreatment of sophorolipid fermentation liquid and the separation and purification of sophorolipids with different structures. Through a series of steps, the above problems are preliminarily solved, so that the amount of organic solvent used is low, the required factory equipment is relatively conventional, and the treatment process is relatively simple, so as to realize the large-scale industrial production of sophorolipid products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for pre-treating sophorolipid fermentation solution and separating and purifying sophorolipid with different structures
  • Method for pre-treating sophorolipid fermentation solution and separating and purifying sophorolipid with different structures
  • Method for pre-treating sophorolipid fermentation solution and separating and purifying sophorolipid with different structures

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of sophorolipid fermentation broth and determination of sophorolipid yield:

[0046] Fermentation of sophorolipids: carried out in a ventilated stirring fermenter with a volume of 50 L. The liquid volume of the medium is 80% (40L), and the initial dissolved oxygen is 60% (V 氧气 / V 空气 ), the stirring speed is 200rpm, the air flow rate is 0.3m 3 / h. The formula of the fermentation medium is as follows: 6.0% of glucose, 0.3% of yeast powder, 0.1% of potassium dihydrogen phosphate, 0.1% of disodium hydrogen phosphate dodecahydrate, 0.05% of magnesium sulfate heptahydrate, and 6.0% of rapeseed oil. Silicone oil defoamer 1ml. Natural pH fermentation was carried out for 7 days, and samples were taken every 8 hours to measure indicators such as biomass, residual sugar, and sophorolipid production.

[0047] Determination of sophorolipid yield:

[0048] DNS method was used to determine the residual amount of glucose in the fermentation broth, and anthrone-sulfuri...

Embodiment 2

[0050] A method for pretreatment of sophorolipid fermentation broth and separation and purification of sophorolipids with different structures, comprising the following steps:

[0051] (1) Pretreatment of fermentation broth

[0052] After the fermentation, the fermented broth with a total sophorolipid content of 117.25g / L, a lactone-type sophorolipid content of 48.88g / L, and an acid-type sophorolipid content of 68.37g / L was first raised to 50°C Sterilize for 40 minutes, cool down naturally for 1.5 hours, and at the same time turn off the stirring and reduce the air flow;

[0053] (2) Collection and processing of lower lactone-type sophorolipids

[0054] Collect the fermented liquid into a cylindrical container, connect it with a chromatographic column with a height of 30 cm and a diameter of 2.5 cm, and collect the viscous lactone-type sophorolipid in the lower layer after standing for 20 minutes; the fermented liquid in the upper layer is set aside;

[0055] (3) Plate and f...

Embodiment 3

[0070] A method for pretreatment of sophorolipid fermentation broth and separation and purification of sophorolipids with different structures, comprising the following steps:

[0071] (1) Pretreatment of fermentation broth

[0072] After the fermentation, the fermentation liquid with a total sophorolipid content of 62.95g / L, a lactone-type sophorolipid content of 36.52g / L, and an acid-type sophorolipid content of 26.43g / L was first raised to 50°C Sterilize for 40 minutes, cool down naturally for 1.5 hours, and at the same time turn off the stirring and reduce the air flow;

[0073] (2) Collection and processing of lower lactone-type sophorolipids

[0074] Collect the fermented liquid into a cylindrical container, connect it with a chromatographic column with a height of 30 cm and a diameter of 2.5 cm, and collect the viscous lactone-type sophorolipid in the lower layer after standing for 20 minutes; the fermented liquid in the upper layer is set aside;

[0075] (3) Plate an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
recovery rateaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for pre-treating a sophorolipid fermentation solution and separating and purifying sophorolipid with different structures. The method comprises the following steps of:pre-treating the sophorolipid fermentation solution; collecting and treating lower-layer lactone type sophorolipid; removing thalli through plate and frame filtering; carrying out resin adsorption and ultra-filtration for impurity removal; carrying out nanofiltration concentration on acidic type sophorolipid; drying. According to the method disclosed by the invention, lactone type, acidic type and mixed type sophorolipid liquid and solid products are obtained respectively by selectively adopting the steps. A traditional ethyl acetate extraction method is replaced with a natural sedimentationmethod and a traditional laboratory centrifuging method is replaced with a common plate and frame filtering method in industrial production to remove mycelia; a common organic solvent extraction method and an alcohol sedimentation method in a laboratory and a production process are replaced with the resin adsorption and ultra-filtration method to remove impurities, and the sophorolipid with quality standards better than those of related commercial products is successfully obtained. According to the method disclosed by the invention, the dosage of organic reagents is greatly reduced and the safety and environmental friendliness of the production of the sophorolipid are improved.

Description

technical field [0001] The invention belongs to the technical field of preparation and purification of biosurfactant sophorolipids, and in particular relates to a method for pretreatment of sophorolipid fermentation liquid and separation and purification of sophorolipids with different structures. Background technique [0002] Sophorolipids, as a class of biosurfactants with excellent surface / interface / bacteriostatic / bactericidal / antitumor / antiviral activities, have great development potential and application prospects in the fields of medicine and other fields. Sophorolipids naturally synthesized by microorganisms are a mixture of a series of structural analogs, which are mainly divided into two categories: acid type and lactone type. Generally speaking, lactone-type sophorolipids have high lipophilicity, have the ability to reduce the surface tension of liquids, and have high antibacterial, bactericidal, and anti-tumor activities; acid-type sophorolipids have better water ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/04C07H1/06
CPCC07H1/06C07H15/04
Inventor 马晓静孟莉周如国于泽权姚日生
Owner HEFEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com