37 results about "Trichosporon" patented technology

The invention relates to a method for cultivating a fungus capable of poisoning and killing plant parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The Trichoderma strain Snef85 involved in the present invention is Trichoderma harzianum, which has been preserved on October 30, 2007 in the General Microorganism Center of China Committee for the Collection of Microorganisms (address: Datun Road, Chaoyang District, Beijing, postal code 100101), The deposit number is CGMCC No.2235. The taxonomic status of the Trichoderma strain Snef85 is Deuteromycotina, Hyphomycetes, Trichosporales, Trichosporaceae, Trichoderma, and Trichoderma harzianum. It is prepared by liquid fermentation culture, and its metabolites have high toxicity to plant parasitic nematodes, especially root-knot nematodes incognita, rice stem-point nematodes and soybean cyst nematodes, and outstanding nematicidal effect. The fermentation broth of the strain has obvious control effects on root-knot nematode (Meloidogynes pp.), rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines). There are significant differences between the treatment of fermented liquid with different dilution concentrations and the treatment of aseptic water, and the nematode killing effect of fermented liquid can reach more than 95%, which has good application and development prospects.

The invention discloses a high-efficiency Metarrhizium anisopliae strain and applications thereof. The strain MaB08 belongs to Eumycota, Mitotic spore fungi, Hyphomycetes, Hyphomycetales, Trichosporon and Metarrhizium, and is preserved in China Center for Type Culture Collection with the preservation date of October 27th, 2008 and the preservation number of CCTCC M 208172. The high-efficiency Metarrhizium anisopliae is an entomopathogenic fungus that has high efficiency for controlling brontispa longissima, and as a living biological pesticide, the high-efficiency Metarrhizium anisopliae has brand new action mechanism that is different from the existing chemical pesticides, has characteristics of non-pollution and non-residue to environment, and can fit for the requirements of organic food production.

The invention relates to the field of microorganisms, and particularly discloses a low temperature efficient corn stalk degradation composite microbial system and an application thereof. The composite microbial system comprises bacteria and fungi. The bacteria comprise a Cellvibrio bacterial genus, an Azospira bacterial genus, an Arcobacter bacterial genus, an Azonexus bacterial genus, a Clostridium bacterial genus and a Paludibacter bacterial genus. The fungi comprise a Lotharella bacterial genus and a Trichosporon bacterial genus. According to the low temperature efficient corn stalk degradation composite microbial system and the application thereof, the bacterial genera with specific components are used as bacterium source samples, culture media adaptive to the system are adopted, the composite microbial system which is composed of the specific bacterial genera is screened and acclimated through enrichment culture, subculture, enzyme production living bacterium culture and corn stalk degradation culture, corn stalks can be efficiently degraded under a low temperature condition through the system, and the defect that an existing microorganism bacterium agent can only degrade the crop stalks under a medium-high temperature condition is overcome.

The subject invention is directed to the treatment of infections caused by fungi which are resistant to standard antifungal drugs such as fluconazole. The treatment method involves administering to the sufferer an effective amount of an agent that suppresses the activity of the fungal (H+)-ATPase pump (Pma1p). Such agents may take the form of: a) 2-phenyl-1,2-benzisoselenazol-3(2H)-one, commonly referred to as ebselen and depicted by Formula I, or b) 2-phenyl-1,2-benzisoselenazol-3(2H)-one 1-oxide, commonly referred to as ebselen se-oxide and depicted by Formula II, or pharmaceutically acceptable salts thereof. Opportunistic fungal infections include those which are systemic and those which are superficial and are caused by species of fungi belonging to the genus Candida, Aspergillus, Pneumocystis, Tinea, Blastomyces, Cryptococcus, Histoplasma, Sporotrichum, Malassezia, Hansenula, Rhodotorula, Trichosporon, as well as others.

The invention discloses oleaginous yeast and application thereof, and belongs to the technical field of microorganisms. The Trichosporon delbrueckii provided by the invention is preserved in the ChinaCenter for Type Culture Collection on May 21, 2020, and the preservation number of the Trichosporon delbrueckii is CCTCC NO: M2020139. According to the oleaginous yeast provided by the invention, cheap hydrolysate of lignocellulose biomass can be used as a substrate, and meanwhile, xylose and glucose in the hydrolysate are used for producing grease, so that sugar utilization efficiency and production strength are improved, and after fed-batch fermentation is carried out for 8 days, the grease yield can reach 31.33 g/L, and the grease content is 60.83%. Therefore, waste resources can be recycled, production cost is reduced, and the oleaginous yeast has a wide application prospect.

The invention provides a paddy field, crayfish and loach coupling planting and breeding method. The method comprises the following steps that a field is selected, ditches are arranged on the periphery of the field, and a crayfish A area, a loach area and a crayfish B area are arranged in the field; the crayfish A area and the crayfish B area are each provided with a plurality of crayfish ditches; the loach area is provided with a plurality of mud holes; rice seedlings are planted in the crayfish A area, the loach area and the crayfish B area respectively, fertilizer I is applied 3-7 days later, and loach larvae are put 25-35 days after the rice seedlings are planted; 40-45 days after the rice seedlings are planted, crayfish larvae are put; a complex microbial agent is sprayed every 25-35 days, the complex microbial agent is prepared from bacillus subtilis powder, trichosporon and lactobacillus powder, and the crayfish and the loach are harvested after the rice is harvested in the rice harvesting period. By means of the planting and breeding method, growth of the crayfish, the loach and the rice is promoted mutually, the yield is remarkably increased, meanwhile, the specifications of the crayfish and the loach are improved, the loach and the crayfish are easy to capture, and the yield per mu is remarkably increased.

Through amplifying lipomyces starkeyi 3-phosphoglyceric kinase genome DNA upstream and downstream sequences and carrying out biology information analysis and functional verification, a promoter and terminator that can be widely applied to gene expression, genetic engineering operation, and strain improvement of Lipomyces and Trichosporon can be obtained. The nucleotide sequence of the promoter and terminator are represented by the SEQ ID No.1 and SEQ ID No.2. The invention also relates to a DNA expression cassette or recombinant carrier comprising the elements, a method using related elements to construct gene engineering strains of Lipomyces or Trichosporon, and corresponding strains.

The invention provides a promoter and terminator of 3-phosphoglyceraldehyde dehydrogenase. The promoter and terminator are obtained by amplifying the upstream and downstream sequences of the genomic DNA of 3-phosphoglyceraldehyde dehydrogenase of Lipomyces starkeyi and carrying out biological information analysis and function verification and can be widely used for gene expression, genetic engineering manipulation and strain improvement of Lipomyces and Trichosporon among Oleaginous yeasts. The nucleotide sequences of the promoter and terminator are SEQ ID No. 1 and SEQ ID No. 2, respectively. The invention also relates to DNA expression cassettes or recombinant vectors containing the promoter and terminator, and methods and corresponding bacterial strains used for constructing Lipomyces or Trichosporon gene engineering bacterial strains by using the promoter and terminator.

The invention relates to a fermentation method of herbal trichosporon kashiwayama with a spot fading effect. The method comprises the following steps of pretreating and homogenizing raw materials, adding trichosporon kashiwayama for fermentation, and then performing filtering and concentrating to obtain a fermentation product; wherein in the homogenizing treatment, a micro-jet high-pressure homogenizer is adopted, and suction feeding is performed by a booster pump or a homogenizer plunger pump; the materials are pressurized to a set pressure value of 20 Mpa to 40 Mpa in the plunger booster pump, and the real-time temperature control temperature range of the materials is set to be 35 DEG C-42 DEG C. According to the technical scheme of the invention, sterilization is carried out through homogenization treatment and a microporous membrane, traditional steps are replaced, fermentation is carried out through known trichosporon kashiwayama, the operation method is simple, and the cost is low; and the obtained yeast microbial fermentation product has high anti-inflammatory activity and can be used for preparing medicines, cosmetics or skin care products with the spot fading effect.

The invention discloses an application of Trichosporon loubiereii in selective catalysis of citral to generate dextro-citronellal, and the preservation number of the Trichosporon loubiereii is CGMCC (China General Microbiological Culture Collection Center) 1292. The invention further discloses a preparation method of the Trichosporon loubiereii. The invention further discloses a method for preparing the L-isopulegol. According to the present invention, the citral olefinic bond reductase catalyst derived from the lebuliferae trichosporon CGMCC 1292 has high stereoselectivity on cis/trans-citral, and the optical purity of the product d-citronellal is superior to the optical purity of the product d-citronellal reported at present. On the basis, a citral olefinic bond reductase reaction step and a metal catalytic cyclization reaction step of the product dextro-citronellal are organically coupled in series, so that the consumption of an organic solvent is reduced, the process is simpler, the optical purity of the product is good, and the industrial application prospect is better.

The invention discloses a method for converting crude fibers of rice straws into crude fat through grease microbial fermentation. In order to reduce the crude fiber of the rice straw and increase the crude fat content of the rice straw, the feed is prepared by taking the rice straw as a substrate and assisting agricultural by-products such as wheat bran, rapeseed meal and peeled sweet potato powder through grease microorganism semi-solid state fermentation. According to a single-factor test scheme, fermentation microorganisms including fermentative trichosporon CICC1368, mortierella chrysosphaerella As3.3410, saccharomyces cerevisiae YJZP01 and rhodotorula graminis H124 are subjected to single-factor test optimization, a fermentation substrate is rice straw as a main material, wheat bran, rapeseed meal or peeled sweet potatoes as an auxiliary material, and the proportion of the auxiliary material is (0 to 10 percent, 20 percent, 30 percent, 40 percent and 50 percent). The method comprises the following steps: carrying out an orthogonal test on fermentation condition loading capacity (20, 50, 80g/250mL triangular flask), material-to-water ratio (0.50, 1.00, 1.50), fermentation days (5, 10, 20%), inoculation proportion (5, 10, 20%) and addition of citric acid (0.2, 0.5, 0.8%), KH2PO4 (0.1, 0.2, 0.3%), urea (0.2, 0.5, 0.8%) and calcium chloride (0.01, 0.02, 0.03%) to fermentation substrates, and obtaining the optimal fermentation condition by taking the crude fat increasing range as a target. Under the optimal condition, the crude fat content of the rice straw is increased by 0.603% from 0.111%, and the increase rate reaches 5 times.

A polyester containing aromatic moieties is contacted with microorganisms having the activity of decomposing the polyester to decompose or reduce it. Preferably, either or both of Trichosporon FERM BP-6445 or Arthrobacter FERM BP-6444 was contacted with the polyester to decompose or reduce it. A fiber made of the polyester or a cloth made of such fiber may be reduced by contacting it with the microorganisms having the activity of decomposing the polyester.

The airlift reactor comprises an outer cylinder, a guide pipe is arranged in the outer cylinder, a sealing cover is arranged at a cylinder opening of the outer cylinder, a liquid outlet and a gas distributor are arranged in the sealing cover, the gas distributor is arranged at a lower end opening of the guide pipe, and the liquid outlet and the gas distributor are communicated with each other. A spiral plate is arranged in the guide pipe, and a liquid inlet is formed in the bottom of the outer cylinder. The airlift reactor for treating the soybean oil refining wastewater by the fermentative trichosporon is used for realizing treatment and resource utilization of the soybean oil refining wastewater and improving the wastewater treatment efficiency.

A microorganism capable of degrading an estrogenic substance contained in wastewater and the like; a method for the simple and effective degradation of an estrogenic substance contained in domestic wastewater, wastewater discharged from a livestock farm, or the like by using the microorganism; and an apparatus for achieving the degradation are provided. An estrogenic substance-degrading microorganism which belongs to the genus Pseudaminobacter, Gordonia, Nocardia, Zoogloea, Pandoraea, Cryptococcus or Trichosporon and has an ability to degrade an estrogenic substance; a method for the degration of an estrogenic substance by using the microorganism; and an apparatus for achieving the degradation are provided.