37 results about "Trichosporon" patented technology

The invention belongs to microbial fermentation, and particularly relates to a preparation method of a probiotic inoculum for preventing replant disease of greenhouse cucumbers. The preparation method comprises a fermentation step: inoculating a probiotic inoculum producing strain into a sterile culture medium containing a carbon source, a nitrogen source, inorganic salt and water; carrying out aerobic fermentation under the conditions that the temperature is 30-37 DEG C and the pH value is 7.2-7.6 in the fermentation process, wherein the producing strain is a mixture of Bacillus subtilis, Bacillus licheniformis, Trichosporon behrendii, Paecilomyces lilacinus, Streptomyces microflavus, Bacillus polymyxa and Metarhizium anisopliae of the same mass; and adsorbing the final fermentation product, and immobilizing on grass carbon to obtain the probiotic inoculum. The preparation method solves the defects of replant disease, root rot, blight, nematode disease and the like in the existing greenhouse vegetable-cucumber planting technology, has the advantages of low production cost, short fermentation period and high flora density, and can effectively prevent seedling death, seedling yellowing, blight, mosaic disease, root rot and other ecological symptoms of soil. Meanwhile, the preparation method can promote the growth of roots, loosen the soil, strengthen the seedlings and degrade the residual pesticides and fertilizers, and has an effect of obviously restoring the soil ecosystem. By using the preparation method, the yield of greenhouse cucumbers can be increased by 15-20% in one season.

The invention provides methods and materials related to the production of lactic acid. Specifically, the invention provides methods for producing lactic acid using a crabtree-negative yeast, such as of the Kluyveromyces, Pichia, Candida, Trichosporon and Yamadazmya genera, which have been transformed with a lactate dehydrogenase gene.

The invention relates to a method for cultivating a fungus capable of poisoning and killing plant parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The Trichoderma strain Snef85 involved in the present invention is Trichoderma harzianum, which has been preserved on October 30, 2007 in the General Microorganism Center of China Committee for the Collection of Microorganisms (address: Datun Road, Chaoyang District, Beijing, postal code 100101), The deposit number is CGMCC No.2235. The taxonomic status of the Trichoderma strain Snef85 is Deuteromycotina, Hyphomycetes, Trichosporales, Trichosporaceae, Trichoderma, and Trichoderma harzianum. It is prepared by liquid fermentation culture, and its metabolites have high toxicity to plant parasitic nematodes, especially root-knot nematodes incognita, rice stem-point nematodes and soybean cyst nematodes, and outstanding nematicidal effect. The fermentation broth of the strain has obvious control effects on root-knot nematode (Meloidogynes pp.), rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines). There are significant differences between the treatment of fermented liquid with different dilution concentrations and the treatment of aseptic water, and the nematode killing effect of fermented liquid can reach more than 95%, which has good application and development prospects.

The invention discloses a high-efficiency Metarrhizium anisopliae strain and applications thereof. The strain MaB08 belongs to Eumycota, Mitotic spore fungi, Hyphomycetes, Hyphomycetales, Trichosporon and Metarrhizium, and is preserved in China Center for Type Culture Collection with the preservation date of October 27th, 2008 and the preservation number of CCTCC M 208172. The high-efficiency Metarrhizium anisopliae is an entomopathogenic fungus that has high efficiency for controlling brontispa longissima, and as a living biological pesticide, the high-efficiency Metarrhizium anisopliae has brand new action mechanism that is different from the existing chemical pesticides, has characteristics of non-pollution and non-residue to environment, and can fit for the requirements of organic food production.

The invention discloses a manufacturing method of low-temperature lipase through Trichosporon pullulans, which is characterized by the following: utilizing Trichosporon pullulans (CGMCC. No. 1674) to build bacterial preservation, rejuvenated and breeding system; optimizing the sieving through culture medium formula; controlling the fermenting technique; manufacturing the product with good low-temperatur activity and high-enzyme activity; fitting for washing, edible manufacturing, leather manufacturing, help digest agent, organic synthetic chemical product, perfume, drug preparation, fat manufacturing domain widely.

The invention discloses a method for producing astragalus notoginseng mycoplasm by cordyceps-related fungi co-fermentation and application thereof. The technical scheme adopted includes: preparation of solid fermentation culture medium of traditional Chinese medicine, preparation of liquid culture medium, culture of liquid mother species, co-fermentation and transformation, drying of fermentationproducts, and ultrafine grinding. The bacterial substance cultured according to the technical scheme of the invention comprises astragalus membranaceus or notoginseng, All active constituents of Paecilomyces dauricus and Trichosporon chinensis, in a co-fermentation proces, Chinese medicinal herbs can activate the expression of silent secondary metabolic biosynthetic gene clusters of fungi, The invention simulates the formation process of natural Cordyceps sinensis, and converts radix astragali and radix notoginseng. Therefore, the bacterial quality cultured in the technical scheme of the invention is high in efficiency, stable, and the co-fermentation product has good immune enhancement effect, and the curative effect is good, which is far superior to the simple compatibility compound combination.

The invention relates to the field of microorganisms, and particularly discloses a low temperature efficient corn stalk degradation composite microbial system and an application thereof. The composite microbial system comprises bacteria and fungi. The bacteria comprise a Cellvibrio bacterial genus, an Azospira bacterial genus, an Arcobacter bacterial genus, an Azonexus bacterial genus, a Clostridium bacterial genus and a Paludibacter bacterial genus. The fungi comprise a Lotharella bacterial genus and a Trichosporon bacterial genus. According to the low temperature efficient corn stalk degradation composite microbial system and the application thereof, the bacterial genera with specific components are used as bacterium source samples, culture media adaptive to the system are adopted, the composite microbial system which is composed of the specific bacterial genera is screened and acclimated through enrichment culture, subculture, enzyme production living bacterium culture and corn stalk degradation culture, corn stalks can be efficiently degraded under a low temperature condition through the system, and the defect that an existing microorganism bacterium agent can only degrade the crop stalks under a medium-high temperature condition is overcome.

The subject invention is directed to the treatment of infections caused by fungi which are resistant to standard antifungal drugs such as fluconazole. The treatment method involves administering to the sufferer an effective amount of an agent that suppresses the activity of the fungal (H+)-ATPase pump (Pma1p). Such agents may take the form of: a) 2-phenyl-1,2-benzisoselenazol-3(2H)-one, commonly referred to as ebselen and depicted by Formula I, or b) 2-phenyl-1,2-benzisoselenazol-3(2H)-one 1-oxide, commonly referred to as ebselen se-oxide and depicted by Formula II, or pharmaceutically acceptable salts thereof. Opportunistic fungal infections include those which are systemic and those which are superficial and are caused by species of fungi belonging to the genus Candida, Aspergillus, Pneumocystis, Tinea, Blastomyces, Cryptococcus, Histoplasma, Sporotrichum, Malassezia, Hansenula, Rhodotorula, Trichosporon, as well as others.

The invention discloses oleaginous yeast and application thereof, and belongs to the technical field of microorganisms. The Trichosporon delbrueckii provided by the invention is preserved in the ChinaCenter for Type Culture Collection on May 21, 2020, and the preservation number of the Trichosporon delbrueckii is CCTCC NO: M2020139. According to the oleaginous yeast provided by the invention, cheap hydrolysate of lignocellulose biomass can be used as a substrate, and meanwhile, xylose and glucose in the hydrolysate are used for producing grease, so that sugar utilization efficiency and production strength are improved, and after fed-batch fermentation is carried out for 8 days, the grease yield can reach 31.33 g / L, and the grease content is 60.83%. Therefore, waste resources can be recycled, production cost is reduced, and the oleaginous yeast has a wide application prospect.

The subject invention is directed to the treatment of infections caused by fungi which are resistant to standard antifungal drugs such as fluconazole. The treatment method involves administering to the sufferer an effective amount of an agent that suppresses the activity of the fungal (H+)-ATPase pump (Pma1p). Such agents may take the form of: a) 2-phenyl-1,2-benzisoselenazol-3(2H)-one, commonly referred to as ebselen and depicted by Formula I, or b) 2-phenyl-1,2-benzisoselenazol-3(2H)-one 1-oxide, commonly referred to as ebselen se-oxide and depicted by Formula II, or pharmaceutically acceptable salts thereof. Opportunistic fungal infections include those which are systemic and those which are superficial and are caused by species of fungi belonging to the genus Candida, Aspergillus, Pneumocystis, Tinea, Blastomyces, Cryptococcus, Histoplasma, Sporotrichum, Malassezia, Hansenula, Rhodotorula, Trichosporon, as well as others.

The invention provides a paddy field, crayfish and loach coupling planting and breeding method. The method comprises the following steps that a field is selected, ditches are arranged on the periphery of the field, and a crayfish A area, a loach area and a crayfish B area are arranged in the field; the crayfish A area and the crayfish B area are each provided with a plurality of crayfish ditches; the loach area is provided with a plurality of mud holes; rice seedlings are planted in the crayfish A area, the loach area and the crayfish B area respectively, fertilizer I is applied 3-7 days later, and loach larvae are put 25-35 days after the rice seedlings are planted; 40-45 days after the rice seedlings are planted, crayfish larvae are put; a complex microbial agent is sprayed every 25-35 days, the complex microbial agent is prepared from bacillus subtilis powder, trichosporon and lactobacillus powder, and the crayfish and the loach are harvested after the rice is harvested in the rice harvesting period. By means of the planting and breeding method, growth of the crayfish, the loach and the rice is promoted mutually, the yield is remarkably increased, meanwhile, the specifications of the crayfish and the loach are improved, the loach and the crayfish are easy to capture, and the yield per mu is remarkably increased.

Through amplifying lipomyces starkeyi 3-phosphoglyceric kinase genome DNA upstream and downstream sequences and carrying out biology information analysis and functional verification, a promoter and terminator that can be widely applied to gene expression, genetic engineering operation, and strain improvement of Lipomyces and Trichosporon can be obtained. The nucleotide sequence of the promoter and terminator are represented by the SEQ ID No.1 and SEQ ID No.2. The invention also relates to a DNA expression cassette or recombinant carrier comprising the elements, a method using related elements to construct gene engineering strains of Lipomyces or Trichosporon, and corresponding strains.

The invention provides a promoter and terminator of 3-phosphoglyceraldehyde dehydrogenase. The promoter and terminator are obtained by amplifying the upstream and downstream sequences of the genomic DNA of 3-phosphoglyceraldehyde dehydrogenase of Lipomyces starkeyi and carrying out biological information analysis and function verification and can be widely used for gene expression, genetic engineering manipulation and strain improvement of Lipomyces and Trichosporon among Oleaginous yeasts. The nucleotide sequences of the promoter and terminator are SEQ ID No. 1 and SEQ ID No. 2, respectively. The invention also relates to DNA expression cassettes or recombinant vectors containing the promoter and terminator, and methods and corresponding bacterial strains used for constructing Lipomyces or Trichosporon gene engineering bacterial strains by using the promoter and terminator.

The invention relates to a fermentation method of herbal trichosporon kashiwayama with a spot fading effect. The method comprises the following steps of pretreating and homogenizing raw materials, adding trichosporon kashiwayama for fermentation, and then performing filtering and concentrating to obtain a fermentation product; wherein in the homogenizing treatment, a micro-jet high-pressure homogenizer is adopted, and suction feeding is performed by a booster pump or a homogenizer plunger pump; the materials are pressurized to a set pressure value of 20 Mpa to 40 Mpa in the plunger booster pump, and the real-time temperature control temperature range of the materials is set to be 35 DEG C-42 DEG C. According to the technical scheme of the invention, sterilization is carried out through homogenization treatment and a microporous membrane, traditional steps are replaced, fermentation is carried out through known trichosporon kashiwayama, the operation method is simple, and the cost is low; and the obtained yeast microbial fermentation product has high anti-inflammatory activity and can be used for preparing medicines, cosmetics or skin care products with the spot fading effect.

The invention discloses an application of Trichosporon loubiereii in selective catalysis of citral to generate dextro-citronellal, and the preservation number of the Trichosporon loubiereii is CGMCC (China General Microbiological Culture Collection Center) 1292. The invention further discloses a preparation method of the Trichosporon loubiereii. The invention further discloses a method for preparing the L-isopulegol. According to the present invention, the citral olefinic bond reductase catalyst derived from the lebuliferae trichosporon CGMCC 1292 has high stereoselectivity on cis / trans-citral, and the optical purity of the product d-citronellal is superior to the optical purity of the product d-citronellal reported at present. On the basis, a citral olefinic bond reductase reaction step and a metal catalytic cyclization reaction step of the product dextro-citronellal are organically coupled in series, so that the consumption of an organic solvent is reduced, the process is simpler, the optical purity of the product is good, and the industrial application prospect is better.

The invention discloses a method for converting crude fibers of rice straws into crude fat through grease microbial fermentation. In order to reduce the crude fiber of the rice straw and increase the crude fat content of the rice straw, the feed is prepared by taking the rice straw as a substrate and assisting agricultural by-products such as wheat bran, rapeseed meal and peeled sweet potato powder through grease microorganism semi-solid state fermentation. According to a single-factor test scheme, fermentation microorganisms including fermentative trichosporon CICC1368, mortierella chrysosphaerella As3.3410, saccharomyces cerevisiae YJZP01 and rhodotorula graminis H124 are subjected to single-factor test optimization, a fermentation substrate is rice straw as a main material, wheat bran, rapeseed meal or peeled sweet potatoes as an auxiliary material, and the proportion of the auxiliary material is (0 to 10 percent, 20 percent, 30 percent, 40 percent and 50 percent). The method comprises the following steps: carrying out an orthogonal test on fermentation condition loading capacity (20, 50, 80g / 250mL triangular flask), material-to-water ratio (0.50, 1.00, 1.50), fermentation days (5, 10, 20%), inoculation proportion (5, 10, 20%) and addition of citric acid (0.2, 0.5, 0.8%), KH2PO4 (0.1, 0.2, 0.3%), urea (0.2, 0.5, 0.8%) and calcium chloride (0.01, 0.02, 0.03%) to fermentation substrates, and obtaining the optimal fermentation condition by taking the crude fat increasing range as a target. Under the optimal condition, the crude fat content of the rice straw is increased by 0.603% from 0.111%, and the increase rate reaches 5 times.

A polyester containing aromatic moieties is contacted with microorganisms having the activity of decomposing the polyester to decompose or reduce it. Preferably, either or both of Trichosporon FERM BP-6445 or Arthrobacter FERM BP-6444 was contacted with the polyester to decompose or reduce it. A fiber made of the polyester or a cloth made of such fiber may be reduced by contacting it with the microorganisms having the activity of decomposing the polyester.

In the present invention, by amplifying the upstream and downstream sequences of 3-glyceraldehyde phosphate dehydrogenase genome DNA of Lipomyces starii, and performing biological information analysis and functional verification, the invention can be widely used in oleaginous yeast Lipomyces (Lipomyces) and silk A promoter and a terminator for gene expression, genetic engineering operation and strain improvement in Trichosporon, the nucleotide sequences of which are SEQ ID NO: 1 and SEQ ID NO: 2, respectively. The present invention also relates to a DNA expression cassette or a recombinant vector containing these elements, and a method for constructing a genetically engineered bacterial strain of the genus Liposaccharomyces or Trichosporon by using the relevant elements and corresponding bacterial strains.

The invention discloses a method for preparing biodiesel through trichosporon cutaneum. The method comprises the following steps: firstly, preparing microorganism grease, namely implementing batch fermentation culture under a certain culture condition by taking a synanthrin extracting solution as a grease producing culture medium and trichosporon cutaneum as a grease producing strain; drying and extracting to obtain microorganism grease; then, preparing biodiesel, namely heating the microorganism grease which serves as a raw material through microwave with presence of a catalyst, and carrying out a transesterification reaction with methanol to obtain the biodiesel. By producing the microorganism grease by virtue of fermentation of the jerusalem artichoke extracting solution, the method disclosed by the invention can be used for overcoming the defect of inulin produced grease which is high in production cost due to addition of a yeast extract, peptone and inorganic salt, obviously reducing the biodiesel production cost and realizing full use of the jerusalem artichoke resource; moreover, the established production process of the biodiesel through microwave heating is simple and convenient to operate, environment-friendly, quick in reaction speed, high in biodiesel yield and easy to control, and the production process has excellent industrial application prospect.

The airlift reactor comprises an outer cylinder, a guide pipe is arranged in the outer cylinder, a sealing cover is arranged at a cylinder opening of the outer cylinder, a liquid outlet and a gas distributor are arranged in the sealing cover, the gas distributor is arranged at a lower end opening of the guide pipe, and the liquid outlet and the gas distributor are communicated with each other. A spiral plate is arranged in the guide pipe, and a liquid inlet is formed in the bottom of the outer cylinder. The airlift reactor for treating the soybean oil refining wastewater by the fermentative trichosporon is used for realizing treatment and resource utilization of the soybean oil refining wastewater and improving the wastewater treatment efficiency.

A microorganism capable of degrading an estrogenic substance contained in wastewater and the like; a method for the simple and effective degradation of an estrogenic substance contained in domestic wastewater, wastewater discharged from a livestock farm, or the like by using the microorganism; and an apparatus for achieving the degradation are provided. An estrogenic substance-degrading microorganism which belongs to the genus Pseudaminobacter, Gordonia, Nocardia, Zoogloea, Pandoraea, Cryptococcus or Trichosporon and has an ability to degrade an estrogenic substance; a method for the degration of an estrogenic substance by using the microorganism; and an apparatus for achieving the degradation are provided.

The invention belongs to the technical field of microorganisms, and discloses a composite microbial preparation which is prepared by the following process: mixing trichoderma koningii-Trichosporon mixed fermentation liquor, rhodopseudomonas palustris fermentation liquor and bacillus subtilis fermentation liquor uniformly according to the volume ratio of 5-8:2-3:2-3, and then performing vacuum freeze drying to obtain bacterial powder, thereby the composite microbial preparation is obtained. According to the invention, four biocontrol strains are adopted in the composite microbial preparation, which can achieve synergistic symbiosis, have reasonable compatibility, and are not antagonistic to each another; the composite microbial preparation can greatly improve the effect of preventing and treating phytophthora, is harmless to people, livestock and crops, is environmentally friendly, has relatively simple preparation process, is convenient and fast to use and has wide application prospect.

The invention relates to malic dehydrogenase A which comes from corbrin-capsule-producing strain cordyceps sinensis hirsutella sinensis, participates tricarboxylic acid cycle and is used for citric acid synthesis, a gene encoding the malic dehydrogenase A, and application of the above two. The amino acid sequence of the malic dehydrogenase A and protein shown as SEQ ID No. 1 have 90% or more of homology, and the nucleotide sequence of the encoding gene and a sequence shown as SEQ ID No. 2 have 90% or more of homology. A cloned DNA of the provided nucleotide sequence can be transferred into an engineering bacterium through a transduction, transformation or conjugal transferring method. By adjusting expression of the encoding gene corresponding to the enzyme catalyzing malic acid for preparing corresponding oxobutanedioic acid, a host is endowed with property of highly expressing malic dehydrogenase A, an effective approach is provided for expanding biological application of malic dehydrogenase A, and important application prospect is provided.

The invention discloses a method for inducing sporulation of hyphosporium fungus, which comprises the following steps: (1) take circular filter paper, evenly cut out more than 4 filter paper holes, lay it flat on a petri dish, add appropriate amount of distilled water to soak, Remove the filter paper after autoclaving. (2) Prepare and sterilize the agar medium, and then add it to the sterilized petri dish, the amount of each petri dish is 15-20ml, and make the agar culture plate after the agar medium is solidified. (3) Spread the sterilized filter paper on the surface of the agar culture plate. (4) Pick an appropriate amount of non-sporogenous hyphomycete fungi and inoculate them on the improved agar culture plate at the center of each filter paper hole. (5) Cover and seal the petri dish, and incubate at room temperature under natural light for 10 to 30 days. The method of the invention has simple operation, mild culture conditions, remarkable effect of inducing sporulation of hyphosporium fungi, is effective for most hyphosporium fungi, conidia are evenly distributed on filter paper, and is convenient for observing sporulation phenotypes.