Efficient Metarhizium strain and use thereof for preventing and treating coconut leaf beetle

A technology of Cocoa and Metarhizium anisopliae, applied in the fields of application, fungi, botanical equipment and methods, etc., can solve the problems of high-efficiency insecticidal strains that have not made major breakthroughs, and achieve the effect of no pollution to the environment

Inactive Publication Date: 2009-07-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as the key technology of fungal insecticides - the breeding of high-efficiency i

Method used

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  • Efficient Metarhizium strain and use thereof for preventing and treating coconut leaf beetle
  • Efficient Metarhizium strain and use thereof for preventing and treating coconut leaf beetle
  • Efficient Metarhizium strain and use thereof for preventing and treating coconut leaf beetle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] By collecting the carcass of a susceptible coconut leaf beetle in Guangzhou (see attached figure 1 And attached figure 2 ), using conventional general techniques to isolate and purify bacterial strains, pathogenicity determination, biological characteristics and production traits comparison, and strain determination, etc., after repeated screening, a strain of Metarhizium anisopliae highly efficient to the coco beetle was obtained, numbered MaB08.

[0027] Preparation of spore suspension: pick spores from the slant bacteria, insert them into 0.05% Tween 80 solution that has been sterilized, measure the spore concentration with a hemocytometer, and adjust to 10 7 Spores / mL.

[0028] Seed culture: inoculate 2 mL of spore suspension into 98 mL of seed medium, shake the flask at 180 r / min and 26 ° C for 2 to 3 days to form a seed liquid.

[0029] Seed medium composition: Czapek-Dox with 0.5% peptone: Peptone 5g, NaNO 3 3g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5...

Embodiment 2

[0034] Example 2 Utilize this bacterial strain to prevent and control the coconut heart leaf beetle

[0035] With reference to the conidia harvested by the method described in Example 1, the Tween 80 solution was used to prepare a concentration of 10 6 ~10 7 Suspension of conidia / mL, the suspension is applied to the field by foliar spraying method, and it is applied at the peak period of occurrence of cocoa beetle larvae.

[0036] Toxicity of the MaB08 strain to the cocoa beetle: under different spore concentrations, the average cumulative mortality of the 2nd instar larvae of the cocoa beetle increased significantly with the increase of the treatment concentration and the prolongation of time, as shown in Table 1. When the concentration was 50000 conidia / mL, the mortality rate of the 2nd instar larvae of the cocoa beetle on the 5th day after handling was more than 50.00%.

[0037] The half-lethal dose was calculated by the logarithmic-probability value analysis method of t...

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Abstract

The invention discloses a high-efficiency Metarrhizium anisopliae strain and applications thereof. The strain MaB08 belongs to Eumycota, Mitotic spore fungi, Hyphomycetes, Hyphomycetales, Trichosporon and Metarrhizium, and is preserved in China Center for Type Culture Collection with the preservation date of October 27th, 2008 and the preservation number of CCTCC M 208172. The high-efficiency Metarrhizium anisopliae is an entomopathogenic fungus that has high efficiency for controlling brontispa longissima, and as a living biological pesticide, the high-efficiency Metarrhizium anisopliae has brand new action mechanism that is different from the existing chemical pesticides, has characteristics of non-pollution and non-residue to environment, and can fit for the requirements of organic food production.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a high-efficiency metarhizium anisopliae strain and its application in preventing and treating cocoa beetle. Background technique [0002] Coconut heart leaf beetle Brontispa longissima (Gestro), also known as coconut long leaf beetle, coconut palm flat leaf beetle, coconut bristly leaf beetle, red chest leaf beetle, etc., belongs to Coleoptera, Chrysomeloides, Hispidae, Subfamily subfamily Anisoderinae, which mainly damage palm plants, is a second-class dangerous pest that is prohibited from entering my country. [0003] Coconut leaf beetle was introduced into Taiwan Province of my country in 1975, and was discovered in Hong Kong in 1988. In August 1999, coconut heart leaf beetle was seized from Washington sunflower imported from Taiwan at the South China Sea port. Since then, there have been many reports of intercepting the insect at various ports in my country...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 任顺祥胡琼波
Owner SOUTH CHINA AGRI UNIV
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