13 results about "Leaf beetle" patented technology

The invention belongs to the technical field of agricultural biology, and relates to a nucleotide for controlling coleopteran pests and a method thereof, in particular to a method for controlling diplodia juncoides by reducing or closing the expression of a target gene combination in the diplodia juncoides by using an RNAi (Ribonucleic Acid Interfere) technology. According to the method, MhCOPI gamma and Mhmi2 are selected as target genes, a target sequence is designed, and the diplodia juncoides is contacted with corresponding dsRNA molecules, so that the growth, development and reproduction of the diplodia juncoides are inhibited, the field number of pests is controlled, and the method is an environment-friendly, efficient and safe prevention and treatment method.

The invention relates to a strain BtJC2602 with high activity on phyllotreta striolata and application of the strain BtJC2602. The bacillus thuringiensis wild strain BtJC2602 is screened out from the environment, and the preservation number of the bacillus thuringiensis wild strain BtJC2602 is CGMCC (China General Microbiological Culture Collection Center) No.33742. The strain BtJC2602 provided by the invention has activity on phyllotreta striolata larvae, and realizes larva control on phyllotreta striolata. The activity of the strain BtJC2602 provided by the invention on larvae is higher than that of the existing strain G033A. The bacterial strain provided by the invention also has very high killing activity on similarly hetidaje.

The application discloses application of gelsevine in inhibition of oviposition of carpophilus humeralis. It is found that gelsevine has excellent inhibitory effect on oviposition of carpophilus humeralis, and can be applied to inhibition of oviposition of carpophilus humeralis, especially to prevention and treatment of carpophilus humeralis, and has certain research value, and provides a new method for prevention and treatment of carpophilus humeralis.

This invention discloses a chitin synthase 1 gene and its application in controlling the two-spotted leaf beetle, belonging to the fields of genetic engineering and biological control technology. The nucleotide sequence of the chitin synthase 1 gene is shown in SEQ ID NO.1. This invention also provides a dsRNA for controlling the two-spotted leaf beetle, transcribed from the nucleotide sequence shown in SEQ ID NO.3. This invention discloses for the first time the chitin synthase 1 gene of the two-spotted leaf beetle, and designs a dsRNA based on this gene capable of controlling the two-spotted leaf beetle, as well as transgenic maize expressing the two-spotted leaf beetle dsRNA. Feeding transgenic maize leaves significantly increases the mortality rate of the two-spotted leaf beetle and reduces the area and severity of leaf damage. This invention provides a new genetic resource and an efficient and environmentally friendly control method for the two-spotted leaf beetle, offering a new option for its control and possessing broad application prospects.

The application discloses a molecular marker of a corn chromosome 3 leaf mite resistance related gene and application thereof. The application provides application of a substance for detecting the genotype of a SNP site Zm000011d042333@161,630,118 in a corn genome in at least one of the following: A1) identifying or assisting in identifying corn leaf mite resistance; A2) breeding corn with high leaf mite resistance; the SNP site Zm000011d042333@161,630,118 is the 26th in sequence 1. The application first discovers the gene Zm000011d042333 related to mite resistance in corn, and identifies the mite resistance of corn according to the genotype of the SNP site on the gene, and designs a KASP marker, which can quickly and accurately determine the genotype of the SNP site, and effectively screen corn resistant plants according to the genotype, and accelerate corn resistance breeding.

The invention belongs to the technical field of paulownia leaf beetle prevention and control, and discloses a paulownia leaf beetle prevention and control method, which comprises the following steps: winter protection: in the dormancy period of paulownia trees in winter, cleaning barks of the paulownia trees, pruning branches, and removing weeds around roots of the paulownia trees; spring prevention: the spring is a growth period and a leaf beetle damage period of the paulownia trees, natural enemies are utilized, larvae of the leaf beetles are prevented and controlled, and fertilization and fertilizer adjustment are performed in a seedling establishment period of the paulownia trees; in summer, the leaf beetles are in the peak period, and the leaf beetles are killed; autumn inspection: inspecting whether the paulownia tree has the leaf beetles or not in autumn when the paulownia tree begins to enter the dormancy period, and timely finding and eliminating the state of damage of the leaf beetles; the comprehensive prevention and treatment method is efficient, economical, practical and sustainable, harm of paulownia leaf beetles can be effectively controlled, withering, deformation, aging and other conditions of paulownia trees are avoided, and the productivity and quality of the paulownia trees are improved.

The invention discloses a method and a special kit for rapidly detecting sodium ion channel K1774N mutation of methrips vigna, and belongs to the technical field of biological detection. The method mainly comprises the following steps: 1) extracting DNA (Deoxyribose Nucleic Acid) of the cowpea 2) carrying out PCR amplification by adopting two groups of primer pairs; (3) result judgment: when a 269bp band appears in amplification of the first group of primer pairs, the detected sodion channel gene mutation site K1774N of the cowpea megthrips spp sample is not mutated; when a 269bp band appears in amplification of the second group of primer pairs, the detected sodion channel gene mutation site K1774N of the cowpea megaluthrips spp sample is mutated, and when no band appears, the detected cowpea megaluthrips spp sample is not mutated. According to the application, the mutation condition of the field cowpea megthrip K1774N mutation site can be quickly and effectively detected, and the real-time state of pyrethroid resistance of the field cowpea megthrip is effectively detected.

The invention relates to an insecticidal protein Rhc as well as an expression vector and application thereof. The three-dimensional structure of the insecticidal protein Rhc comprises two structural domains, and the structural domain I is composed of a plurality of alpha helixes and located at the 6th-175th site of an amino acid sequence; the connecting domain consists of an alpha helix and three anti-parallel beta folds, and is positioned at 176-241 sites of an amino acid sequence; and the structural domain II takes beta folding as a main body and is located at 262-436 sites of the amino acid sequence. The insecticidal protein Rhc is a novel protein, and under the background of a delta cdsR mutant, the promoter P5014 can effectively guide the expression of the Rhc protein; a biological activity test shows that the median lethal concentration (LC50) of Rhc protein expressed under the guidance of P5014 in the delta cdsR mutant to newly hatched larvae of melotidax aeda is 233 [mu] g / mL.

The application discloses a lethal related gene MuSrp54k of a bean leaf beetle and application of the lethal related gene MuSrp54k in prevention and treatment of the bean leaf beetle. The application provides a Serratia marcescens HvSm-1 capable of being used for prevention and treatment of the bean leaf beetle, and a lethal related gene MuSrp54k of the bean leaf beetle, and constructs a dsRNA-dsMuSrp54k for prevention and treatment of the bean leaf beetle by using the lethal related gene MuSrp54k. Further, after the bean leaf beetle is fed by using the dsMuSrp54k and the Serratia marcescens jointly, the corrected mortality of the 2nd instar nymphs is about 88% within 7 days, and the corrected mortality of the adults is about 58% within 7 days, and the synergistic virulence index is used to judge that the virulence of the 2nd instar nymphs and the adults of the bean leaf beetle treated by the dsMuSrp54k and the HvSm-1 jointly produces a synergistic effect. The application provides a safe and efficient method for prevention and treatment of the bean leaf beetle, and has a good application prospect.

This invention relates to the field of tea garden pest control technology, and more particularly to a spraying device for controlling leaf beetles in tea gardens. The device includes a box and a frame embedded and fixed to the top of the box. A liquid inlet pipe connects to the box, and the top of the frame has evenly spaced openings. A collection frame for collecting the killed leaf beetles is placed inside the frame. The invention first activates a servo motor to rotate the T-shaped spray nozzle forward, then activates a water pump to draw pesticide into the outlet pipe. The pesticide in the outlet pipe is sprayed onto the tea leaves through the T-shaped spray nozzle, thus treating the tea leaves and killing the leaf beetles. Simultaneously, a yellow LED light is activated to attract the leaf beetles. Then, an electric screw is activated to move a conical ring downwards. An arc-shaped cover first seals the fixed frame, and the conical ring then removes the leaf beetles from the yellow LED light and moves them into the collection frame for further killing. This prevents the leaf beetles from feigning death and remaining on the tea leaves to further consume them, thereby improving the pest control effect.

The invention relates to an application of leaf alcohol and / or 4-heptanol in inducing diplodia longissima leaf beetles and a preparation method of the leaf alcohol and / or the 4-heptanol. It is found for the first time that both the leaf alcohol and the 4-heptanol can attract the diplodia longifolia, especially when the two compounds are combined together according to a certain proportion to attract the diplodia longifolia, the synergistic interaction effect can be achieved, and therefore the two compounds can be used for monitoring and / or preventing and treating the diplodia longifolia.

This invention provides a chitin synthase 2 gene of the two-spotted leaf beetle and its application, as well as a dsRNA for the control of the two-spotted leaf beetle and its application, relating to the field of biotechnology. This invention uses bioinformatics technology to identify the two-spotted leaf beetle. CHS2 This invention utilizes a gene that, by silencing, can effectively inhibit the growth of the two-spotted firefly beetle, thus achieving control of the beetle. Based on this gene, a dsRNA was designed to mediate the growth of the two-spotted firefly beetle. CHS2 Gene silencing enables specific control of the two-spotted leaf beetle; avoids the toxicity of chemical pesticides to non-target beneficial insects; reduces the use of chemical pesticides and lowers environmental pollution.