The application discloses a lethal
related gene MuSrp54k of a bean
leaf beetle and application of the lethal
related gene MuSrp54k in prevention and treatment of the bean
leaf beetle. The application provides a
Serratia marcescens HvSm-1 capable of being used for prevention and treatment of the bean
leaf beetle, and a lethal
related gene MuSrp54k of the bean leaf beetle, and constructs a dsRNA-dsMuSrp54k for prevention and treatment of the bean leaf beetle by using the lethal related
gene MuSrp54k. Further, after the bean leaf beetle is fed by using the dsMuSrp54k and the
Serratia marcescens jointly, the corrected mortality of the 2nd
instar nymphs is about 88% within 7 days, and the corrected mortality of the adults is about 58% within 7 days, and the synergistic
virulence index is used to judge that the
virulence of the 2nd
instar nymphs and the adults of the bean leaf beetle treated by the dsMuSrp54k and the HvSm-1 jointly produces a synergistic effect. The application provides a safe and efficient method for prevention and treatment of the bean leaf beetle, and has a good application prospect.