Applications of leaf alcohol and / or 4-heptanol in luring dimmosa longissima

Abstract

The invention relates to an application of leaf alcohol and / or 4-heptanol in inducing diplodia longissima leaf beetles and a preparation method of the leaf alcohol and / or the 4-heptanol. It is found for the first time that both the leaf alcohol and the 4-heptanol can attract the diplodia longifolia, especially when the two compounds are combined together according to a certain proportion to attract the diplodia longifolia, the synergistic interaction effect can be achieved, and therefore the two compounds can be used for monitoring and / or preventing and treating the diplodia longifolia.

Description

Technical Field

[0001] The present invention relates to the field of attractants, in particular to application of leaf alcohol and / or 4-heptanol in attracting the two-spotted long-tailed leaf beetle. Background Art

[0002] Due to changes in cultivation systems, crop variety rotation, and global warming, the incidence of the two-spotted long-tarsed leaf beetle on crops in various regions has increased year by year. Existing monitoring and control methods, such as netting and light trapping, are ineffective and difficult to implement. Traditional sticky traps also attract insufficient insects and require further optimization. Therefore, the development of more effective monitoring and control methods is crucial. Summary of the Invention

[0003] One aspect of the present invention provides the use of leaf alcohol and / or 4-heptanol in attracting the two-spotted long-tailed leaf beetle.

[0004] In one embodiment, the leaf alcohol and / or 4-heptanol is used for monitoring and / or controlling the two-spotted long-tailed leaf beetle.

[0005] In a specific embodiment, the insect stage of the two-spotted long-tarsed leaf beetle is an adult.

[0006] In one embodiment, the concentration of the leaf alcohol is 8.325×10 -3 to 8.325×10 -4 mol / L; and / or the concentration of the 4-heptanol is 6.678×10 -7 mol / L.

[0007] The second aspect of the present invention provides a composition comprising leaf alcohol, 4-heptanol and a pesticide-acceptable carrier.

[0008] In one embodiment, the molar ratio of the leaf alcohol to the 4-heptanol is (0.125 to 1250):1.

[0009] In one embodiment, the concentration of the leaf alcohol is 8.325×10 -3 to 8.325×10 -4 mol / L.

[0010] In one embodiment, the concentration of 4-heptanol is 6.678×10 -3 to 6.678×10 - 7 mol / L.

[0011] In one embodiment, the concentration of the leaf alcohol is 8.325×10 -4 mol / L; the concentration of 4-heptanol is 6.678×10-3 to 6.678×10 -7 mol / L.

[0012] In one embodiment, the concentration of the leaf alcohol is 8.325×10 -3 mol / L; the concentration of 4-heptanol is 6.678×10 -3 mol / L.

[0013] In one embodiment, the concentration of the leaf alcohol is 8.325×10 -4 mol / L; the concentration of 4-heptanol is 6.678×10 -3 mol / L.

[0014] In one embodiment, the concentration of the leaf alcohol is 8.325×10 -4 mol / L; and / or the concentration of the 4-heptanol is 6.678×10 -7 mol / L.

[0015] In one embodiment, the composition further comprises a blank rubber head lure.

[0016] In one embodiment, the composition further comprises a yellow sticky insect board.

[0017] In one embodiment, the composition further comprises a solvent.

[0018] In a specific embodiment, the solvent is n-hexane.

[0019] The third aspect of the present invention provides the use of the composition according to any one of the second aspects of the present invention for attracting the two-spotted long-tarsed leaf beetle.

[0020] In one embodiment, the composition is used for monitoring and / or controlling the two-spotted long-tailed leaf beetle.

[0021] In a specific embodiment, the insect stage of the two-spotted long-tarsed leaf beetle is an adult.

[0022] Beneficial effects of the present invention: The present invention discovered for the first time that both leaf alcohol and 4-heptanol can attract the two-spotted long-tarsed leaf beetle, especially when the two compounds are combined in a certain proportion to attract the two-spotted long-tarsed leaf beetle, a synergistic effect can be achieved. Therefore, the two compounds can be used to monitor and / or control the two-spotted long-tarsed leaf beetle. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 Field trapping results for C1 to C7 are shown.

[0024] Figure 2 Field trapping results from D1 to D7 are shown. DETAILED DESCRIPTION

[0025] The above contents of the present invention are further described in detail below in the form of preferred implementation cases, but they do not constitute a limitation of the present invention.

[0026] Unless otherwise specified, the materials and reagents in the examples of the present invention can be purchased through commercial channels.

[0027] Leaf alcohol concentrate (purity 98%, density 0.8508 g / mL) was purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0028] 4-Heptanol concentrate (purity 97%, density 0.8 g / mL) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0029] Dissolve 1 mL of leaf alcohol concentrate in 9 mL of n-hexane to prepare a concentration of 8.325×10 -1 mol / L leaf alcohol solution (denoted as C1); 8.325×10 -1 mol / L leaf alcohol solution was diluted with n-hexane in a gradient manner to obtain a concentration of 8.325×10 - 2 mol / L、8.325×10 -3 mol / L、8.325×10 -4 mol / L、8.325×10 -5 mol / L、8.325×10 -6 mol / L and 8.325×10 -7 mol / L leaf alcohol solution (recorded as C2, C3, C4, C5, C6, C7 accordingly).

[0030] Dissolve 1 mL of 4-heptanol concentrate in 9 mL of n-hexane to prepare a concentration of 6.678×10 -1 mol / L 4-heptanol solution (denoted as D1); 6.678×10 -1 mol / L 4-heptanol solution was diluted with n-hexane in a gradient manner to obtain a concentration of 6.678×10 -2 mol / L、6.678×10 -3 mol / L、6.678×10 -4 mol / L、6.678×10 -5 mol / L、6.678×10 - 6 mol / L and 6.678×10 -7mol / L 4-heptanol solution (recorded as D2, D3, D4, D5, D6, D7 accordingly).

[0031] Take 1ml 8.325×10 -2 mol / L leaf alcohol solution and 1ml 6.678×10 -2 mol / L 4-heptanol solution, the two were mixed, and then the volume was adjusted to 10 ml with n-hexane to obtain a combined C3D3 solution, at which the molar ratio of leaf alcohol to 4-heptanol was 1.25:1.

[0032] Take 1ml 8.325×10 -2 mol / L leaf alcohol solution and 1ml 6.678×10 -6 mol / L 4-heptanol solution, the two were mixed, and then the volume was adjusted to 10 ml with n-hexane to obtain a combined C3D7 solution, at which the molar ratio of leaf alcohol to 4-heptanol was 12500:1.

[0033] Take 1ml 8.325×10 -3 mol / L leaf alcohol solution and 1ml 6.678×10 -2 mol / L 4-heptanol solution, the two were mixed, and then the volume was adjusted to 10 ml with n-hexane to obtain a combined C4D3 solution, at which time the molar ratio of leaf alcohol to 4-heptanol was 0.125:1.

[0034] Take 1ml 8.325×10 -3 mol / L leaf alcohol solution and 1ml 6.678×10 -6 mol / L 4-heptanol solution, the two were mixed, and then the volume was adjusted to 10 ml with n-hexane to obtain a combined C4D7 solution, at which the molar ratio of leaf alcohol to 4-heptanol was 1250:1. Example 1: Field trapping

[0035] The materials involved in the field trapping device are: 25×20 cm yellow board (purchased from Zhongjie Sifang Biotechnology Co., Ltd.), slow-release bait core, and iron wire.

[0036] Preparation method: A blank rubber-tipped lure (purchased from Zhongjie Sifang Biotechnology Co., Ltd.) was immersed in the prepared solution (C1 to C7, D1 to D7, and combinations of C3D3, C3D7, C4D3, and C4D7) for 3 hours and then taken out to make the lure. A thin wire was used to hook the lure and fix the lure in front of a traditional yellow sticky insect board (close to the yellow sticky insect board or with a gap of less than 1 cm from the yellow sticky insect board) to make the sticky insect trap. 2Sticky insect traps were hung on bamboo poles in the corn fields, with each pole 12 m apart. Sampling was carried out using a random stratified sampling method. Sticky insect traps were replaced every 3 days, with 2 to 3 replacements. In case of rain or other extreme weather conditions, the replacement period was extended. The number of insects on the old traps was counted 3 to 4 times during the replacement. The average number of insects trapped on each yellow sticky trap was then calculated for each type of sticky trap. The field trapping results for C1 to C7 are shown in Figure 1. Figure 1 The field trapping results from D1 to D7 are shown in Figure 2 shown.

[0037] Figure 1 The results showed that C3 could significantly attract adults of the two-spotted long-tarsed leaf beetle in the field; Figure 2 The results showed that D7 could significantly attract adults of the two-spotted long-tarsed leaf beetle.

[0038] Based on the book "Experimental Statistical Methods" (Edited by Gai Junyi, published by China Agriculture Press, fourth edition in September 2013, sixth printing in December 2017, ISBN: 978-7-109-17793-2), the expected value formula is: E XY =(A X +A Y )×CF, where E XY A is the expected number of insects attracted by a combination of compound X at a certain concentration and compound Y at a certain concentration, X is the number of insects attracted by compound X at a certain concentration, A Y is the number of insects attracted by compound Y at a certain concentration, CF is the correction factor, CF=0.5. Then the synergistic coefficient is determined based on the expected value and the measured value, that is, SR=A XY / E XY , where SR is the synergistic coefficient, A XY The number of insects attracted by a combination of a certain concentration of compound X and a certain concentration of compound Y is the measured value. When SR>1, it indicates that the combination has a synergistic effect.

[0039] The number of insects attracted, expected values and synergistic coefficients of C3, C4, D3, D7, C3D3, C3D7, C4D3 and C4D7 are shown in Table 1.

[0040] Table 1

[0041] The results in Table 1 show that C3D3, C4D3 and C4D7 have a combined synergistic effect on attracting adults of the two-spotted long-tailed leaf beetle.

Claims

1. Application of leaf alcohol and / or 4-heptanol in attracting the two-spotted long-tailed leaf beetle.

2. The use according to claim 1, characterized in that The leaf alcohol and / or 4-heptanol are used for monitoring and / or controlling the two-spotted long-tailed leaf beetle.

3. The use according to claim 1, characterized in that The insect stage of the two-spotted long-tarsed leaf beetle is an adult.

4. The use according to claim 1, characterized in that The concentration of the leaf alcohol is 8.325×10 -3 to 8.325×10 -4 mol / L; and / or The concentration of 4-heptanol used was 6.678×10 -7 mol / L.

5. A composition comprising leaf alcohol, 4-heptanol and a pesticidally acceptable carrier.

6. The composition according to claim 5, wherein The molar ratio of the leaf alcohol to the 4-heptanol is (0.125 to 1250):

1.

7. The composition according to claim 5, wherein The concentration of the leaf alcohol is 8.325×10 -3 to 8.325×10 -4 mol / L; and / or The concentration of 4-heptanol used was 6.678×10 -3 to 6.678×10 -7 mol / L.

8. Use of the composition according to any one of claims 5 to 7 for attracting the two-spotted long-tailed leaf beetle.

9. The use according to claim 8, characterized in that The composition is used for monitoring and / or controlling the two-spotted long-tailed leaf beetle.

10. The use according to claim 8, characterized in that The insect stage of the two-spotted long-tarsed leaf beetle is an adult.

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