28results about How to "Increased mortality" patented technology

The invention relates to the technical field of biology, and particularly provides preparation and application of a dsMpC002-coated ZIF-8 nano complex. According to the invention, a green peach aphid effector dsMpC002 is taken as a target, a ZIF-8 delivery system of the green peach aphid effector dsMpC002 with a stable superexcellent target is constructed, and a dsMpC002-ZIF-8 nano-composite is prepared and obtained. By observing and analyzing the MpC002 gene expression quantity, the death rate and the single female farrowing quantity of the green peach aphid after the pepper variety DYJ sprayed with the dsMpC002-coated ZIF-8 nano-complex with different concentrations is fed, the control effect of the dsMpC002-coated ZIF-8 nano-complex on the green peach aphid is determined. And a new target technology and a new idea are provided for innovating an aphid control method.

The invention relates to the field of acute lung injury caused by sepsis, in particular to application of lactobacillus rhamnosus exosome in preparation of medicine for treating and / or preventing acute lung injury caused by sepsis of subjects, and the acute lung injury caused by sepsis is acute complication of sepsis. The sepsis is caused by bacterial infection, and the particle size of the lactobacillus rhamnosus exosome ranges from 50 nm to 120 nm. The lactobacillus rhamnosus exosome provided by the invention can prevent and / or timely and effectively prevent the development of the disease course of a subject at the initial stage of sepsis acute lung injury, and finally, the treatment success rate of the subject is increased, and the prognosis of the subject is improved.

The invention relates to the technical field of biological control, and discloses a hyphantria cunea biocontrol bacterium and application thereof, the biocontrol bacterium is classified and named as Entomortierella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella The hyphantria cunea biocontrol bacterium strain provided by the invention can obviously inhibit the survival activity of hyphantria cunea, the death rate of hyphantria cunea larvae can be increased to 96.7% after 7 days of treatment, and the hyphantria cunea larvae can die in the pupal stage and cannot be subjected to eclosion, so that the hyphantria cunea biocontrol bacterium strain can be used for preventing and treating hyphantria cunea, is typical biological prevention and treatment, can avoid the problems of drug resistance, environmental pollution and the like caused by chemical prevention and treatment, and has broad application prospects. Good application prospects are realized.

Provided is a convenient, rapid, and objective blood test-based risk evaluation method for the diagnosis of TIA that can complement the clinical symptom interview, and provided is a convenient, rapid, and objective blood test-based risk evaluation method for the differential diagnosis of cardiogenic and non-cardiogenic acute cerebral vascular diseases such as cerebral infarction and TIA that can complement imaging, electrocardiogram, and conventional blood tests. The concentration of soluble CLEC2 is measured in the blood collected from a patient.

The invention relates to a method for editing target genes of prawn filial generations by exopalaemon carinicauda or vannabine and application of the method. Based on the gonad development and reproduction characteristics of shrimps and crabs, Cas9 RNP (a mixture formed by mixing sgRNA and Cas9 ribonucleoprotein) is directly injected into bodies of exopalaemon carinicauda or litopenaeus vannamei with developed ovaries in an intramuscular injection manner. The used sgRNA is a guide RNA (Ribonucleic Acid), namely EcIAG-gRNA or LvIAG-gRNA, which is designed on the basis of an IAG gene (EcIAG or LvIAG) of exopalaemon carinicauda or litopenaeus vannamei. Different gRNAs and Cas9 proteins are respectively mixed and then injected into an exopalaemon carinicauda or litopenaeus vannamei parent body through intramuscular injection, Cas9 RNP can enter an ovary along with blood circulation and enter an oocyte along with development of the ovary, editing of a target gene on a genome is realized, and after the edited oocyte develops into an ovum, a progeny edited mutant is formed through fertilization. The method can also be used for delivering other biological macromolecular substances to oocytes of decapod crustacean such as exopalaemon carinicauda or litopenaeus vannamei.

The invention discloses application of an epidermal protein gene SlituCP26 in prevention and treatment of prodenia litura, and relates to the technical field of biology. The nucleotide sequence of the SlituCP26 gene is shown as SEQ ID NO: 1, the SlituCP26 gene is highly expressed in an indoxacarb resistant strain, dsRNA of the gene is prepared, the dsRNA is micro-injected into prodenia litura larvae, after injection, the expression level of the SlituCP26 gene is obviously reduced, the body type of the larvae is obviously reduced, the epidermis thickness is obviously reduced, and the sensitivity to indoxacarb is obviously improved. The invention also provides a SlituCP26 gene promoter, and the drug resistance of the prodenia litura can be detected through the difference in sequence after the promoter is amplified. According to the invention, the fact that the epidermal protein SlituCP26 gene participates in the effect of the prodenia litura on resisting insecticides and the formation process of a resistance mechanism is clear, and the epidermal protein SlituCP26 gene has theoretical significance on the development of new insecticides.

The invention discloses a yak intramuscular precursor fat immortalized cell line and a preparation method thereof. The method comprises the following steps: (1) obtaining yak primary intramuscular precursor fat cells; (2) performing SV40T lentivirus infection on the precursor fat cells in the yak muscle and screening cell strains: performing SV40T lentivirus infection by utilizing a 1 / 2 small-volume infection method to obtain immortalized cells after the precursor fat cells in the yak primary muscle are infected with SV40T, namely immortalized cells for short; puromycin is adopted to screen immortalized cells; and diluting the screened cells by using a limited dilution method, and carrying out enlarged culture to obtain the immortalized cell line. The yak intramuscular precursor fat immortalized cell line constructed by the invention has genetic homogeneity, the heterogeneity influence of cell populations is eliminated, and an in-vitro cell model is provided for subsequent research related to yak intramuscular fat cells.

The present invention relates to a new use of rapamycin, and in particular to the use of rapamycin in improving the mosquito-killing activity of Bacillus thuringiensis mosquito-killing toxin. The present invention is the first to apply rapamycin, an immunosuppressant in clinical medicine, to the study of microbial control of pathogenic mosquitoes, and finds that the addition of a low dose of rapamycin can significantly improve the mosquito-killing activity of Bacillus thuringiensis Cry4Ba toxin. The present invention provides an important basis for the subsequent development of new biological pesticides and related small molecule synergists, and is of great significance in improving the activity of microbial pesticides and reducing the cost of use. It also provides a new research direction for studying the insecticidal mechanism of biological control microorganisms represented by Bacillus thuringiensis.

The present invention discloses a miRNA for killing termites and a method for controlling termites by combining the miRNA and green muscardine. The termite control drug contains miR-571 or its agonist as an active ingredient, and the nucleotide sequence of the miR-571 is uuucuguuggugauaucugac. The present invention can effectively increase the mortality rate of termites by injecting or feeding the miR-571 agonist to termites, and injecting or feeding the miR-571 agonist to termites and spraying green muscardine, and the latter causes a higher mortality rate, which can be used as a method for controlling termites.

The application belongs to the field of agricultural biotechnology, and particularly relates to a dsRNA of a brown planthopper gene and application of the dsRNA in preventing and treating the brown planthopper and related methods. NlUGT7 The dsRNA of the gene is prepared through in vitro transcription, and RNA interference treatment is performed on the brown planthopper through microinjection. NlUGT7 The results show that after the dsRNA is injected, the expression amount of the gene in the brown planthopper of biotype Y and biotype 1 is significantly reduced. NlUGT7 After the gene is silenced, the survival rate of the brown planthopper of biotype Y feeding on YHY15 rice containing a Bph15 resistance gene or the brown planthopper of biotype 1 feeding on TN1 rice not containing the resistance gene is significantly reduced. NlUGT7 The gene plays an important role in the survival process of the brown planthopper, and can be used as an effective target for preventing and treating the brown planthopper. NlUGT7 The gene and the dsRNA thereof provided by the application can be applied to research and development of biological pesticides for the brown planthopper and green prevention and control, and have good application prospects.

The invention discloses an NPFR gene-based pomacea canaliculata antifeedant and application thereof, and belongs to the field of prevention and control of agricultural pests. The dsNPFR based on the NPFR gene is designed and synthesized on the basis of an RNAi-mediated NPFR gene silencing technology, by injecting the dsNPFR into the ampullaria gigas, the lethal effect is obvious, the NPFR gene expression quantity, the body weight growth rate, the leaf feeding area, the digestive enzyme activity and the like are remarkably reduced, feeding and growth of the ampullaria gigas are effectively inhibited, a new strategy and a molecular target are provided for research and development of RNAi biopesticides, and the application has a wide application prospect. And a new technical approach is provided for prevention and control of ampullaria gigas invasion hazards.

The invention belongs to the technical field of biological medicines, and particularly relates to application of an mTOR inhibitor to improvement of sleep disorders. Aiming at the defects of the existing hypnotics, the invention finds that the sleep disorder can be improved by using the Rapamycin through researching a new sleep pathway target. The Rapamycin disclosed by the invention is a non-addictive sleeping drug, and can induce natural sleep behaviors; compared with an existing clinical hypnotics which has the side effect of greatly increasing the death rate, the Rapamycin administration disclosed by the invention is beneficial to improving the sleep quality and prolonging the life of animals at the same time. The invention discloses a remarkable effect of Rapamycin in the aspect of improving the sleep disorder, and the discovery has important significance in improving the sleep quality of a patient and preventing the progress of diseases, so that the Rapamycin has a wide medical prospect and a remarkable economic value.

This invention provides the application of the citrus psyllid salivary gland gene DcPS2 in the control of citrus psyllids and Huanglongbing (HLB). The nucleotide sequence of the citrus psyllid salivary gland gene DcPS2 is shown in SEQ ID NO. 1. This invention introduces dsDcPS2 into psyllids and measures the effect of DcPS2 gene silencing on psyllid survival. Experimental results show that after DcPS2 gene silencing, the mortality rate of citrus psyllids significantly increased compared to the control dsGFP, which has significant value for the control of citrus psyllids and the cultivation of psyllid-resistant plants.

The invention discloses combined application of nuclear polyhedrosis virus and bacillus thuringiensis in preparation of a preparation for preventing and treating black cutworm larvae, and relates to the technical field of black cutworm larva prevention and treatment. The matching experiment of different types and different concentrations of nuclear polyhedrosis viruses and bacillus thuringiensis shows that compared with single use of nuclear polyhedrosis viruses or single use of bacillus thuringiensis, the mixed combination of the nuclear polyhedrosis viruses and the bacillus thuringiensis has an obvious synergistic effect in prevention and treatment of young cutworms; the death rate of indoor and field black cutworm larvae can be remarkably increased, the prevention and treatment effect is improved, and a theoretical basis is provided for biological prevention and treatment of black cutworms. Meanwhile, the nuclear polyhedrosis virus and the bacillus thuringiensis are combined for use, so that the problem of < 3 > R caused by chemical pesticide prevention and control can be avoided from the source, and the method is environment-friendly and beneficial to long-term development.

The invention provides an enzyme-responsive nano-drug targeting LRG1 protein, and belongs to the technical field of biological medicines, and the nano-drug is formed by co-assembling an amphiphilic drug conjugate and a carried hydrophobic drug. Wherein the drug conjugate comprises a hydrophilic end of ET peptide targeting LRG1 and a hydrophobic end of the cytotoxic chemotherapeutic drug, the hydrophilic end is covalently linked with the hydrophobic segment through an enzyme-responsive linker, and the hydrophobic drug is entrapped in the amphiphilic drug conjugate. The preparation method comprises the following steps: dissolving the amphipathic drug conjugate and the hydrophobic drug in an organic phase, uniformly mixing the amphipathic drug conjugate and the hydrophobic drug under an ultrasonic condition, dispersing the mixture into a water phase, and standing at room temperature to obtain the targeted response type nano-drug which can prolong the in-vivo circulation time of the drug, realize efficient targeting of a colorectal tumor part, remarkably inhibit the growth of the colorectal tumor of a mouse, and has a good application prospect. DNA damage of tumor cells is promoted, tumor cell apoptosis is induced, and an effective and accurate novel method is provided for enhancing the colorectal cancer treatment effect.

The invention provides a method for separating granular organic matters and mineral binding organic matters in soil, which comprises particle size separation and density separation, and the separation of the granular organic matters and the mineral binding organic matters in the soil is realized by combining the particle size separation and the density separation. The invention discloses a separation device for soil granular organic matters and mineral binding organic matters. The separation device structurally comprises a soil-water mixing module, a screen, a wet screen monitoring module, a fine aggregate collecting box and a density grading pretreatment module, the soil-water mixing module is located above the screen and the wet screening monitoring module, and the screen is arranged between the soil-water mixing module and the wet screening monitoring module to achieve particle size grading of soil particles. The fine aggregate collecting box is located below the wet screening monitoring module, and the fine aggregate collecting box is mounted at the top of the density grading pretreatment module; according to the method, experimental errors caused by only adopting a single particle size grading method or a single density grading method are effectively avoided.

This invention discloses a method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron, belonging to the field of biotechnology. The method includes: a) after acclimatizing 4-5cm largemouth bass to a normal diet for one week, the normal diet is replaced with a FeSO4·7H2O-supplemented diet (FeSO4·7H2O supplementation amount: 800-3200 mg / kg), while the control group continues to be fed a normal diet; b) after feeding the FeSO4·7H2O-supplemented diet for 14-56 days, the mortality rate from Aeromonas hydrophila infection increases, indicating successful establishment of the largemouth bass model susceptibility to pathogens. This method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron has advantages such as simple operation, high success and survival rates, good stability, and obvious pathological characteristics.

The application belongs to the field of biological medicine, and discloses a pharmaceutical composition of water channel protein inhibitor combined with temozolomide for treating brain glioma, wherein the pharmaceutical composition comprises AQP4 inhibitor AER270 and TMZ. The application further discloses a pharmaceutical preparation comprising the above pharmaceutical composition and application of the pharmaceutical preparation in preparation of a medicine for treating brain glioma. The composition provided by the application provides an effective drug combination strategy for treating brain glioma, and it is found for the first time that AER270 and TMZ have a synergistic effect on the treatment of brain glioma.

The invention discloses application of a hydrogel-loaded long-acting insulin receptor antagonist in establishment of a gestational diabetes animal model, 12-week-old C57BL / 6J female mice are divided into two groups, the female mice and male mice are caged and copulated in a ratio of 2: 1, and the gestational condition is observed; from 7 days of pregnancy of a C57BL / 6J female mouse, 60 nmol / kg of an insulin receptor antagonist loaded by phenylboronic acid-hyaluronic acid hydrogel is subcutaneously injected every two days, and the gestational diabetes animal model is established. Different from the characteristics of long modeling time, low success rate, high animal death rate, embryotoxicity in modeling mode, short half-life period of modeling medicine and the like of the conventional gestational diabetes mouse or rat model, the invention establishes a stable and reliable mouse model similar to human pathogenesis, the success rate is high, no obvious toxic effect is generated on a female mouse and an embryo, and the model has a good application prospect. The medicine has a long half-life period and accords with the characteristics of human gestational diabetes.

The invention provides dsRNA (double-stranded ribonucleic acid) of hyphantria cunea HcARNT and HcCncC genes, wherein the full-length cDNA of the HcARNT and the full-length cDNA of the HcCncC genes are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2. The dsHcARNT or the dsHcCncC can silence the expression of the HcARNT or the HcCncC of the fall webworm respectively, interfere the HcARNT gene or the HcCncC gene of the fall webworm, and can enhance the insecticidal effect of tannic acid which is a plant secondary substance. The invention also provides application of the dsRNA in prevention and treatment of fall webworms, and the timely scheme provided by the invention can reduce the use of pesticides, and has the beneficial effects of greenness, environmental protection and the like.

The invention belongs to the field of biological medicine, and discloses a pharmaceutical composition combining an aquaporin inhibitor and temozolomide for treating brain glioma, and the pharmaceutical composition comprises an AQP4 inhibitor AER270 and TMZ. The invention further discloses a pharmaceutical preparation containing the pharmaceutical composition and application of the pharmaceutical preparation to preparation of drugs for treating brain glioma. The composition provided by the invention provides an effective drug combination strategy for treatment of brain glioma, and it is found for the first time that the combination of AER270 and TMZ has a synergistic effect on treatment of brain glioma.

The present invention relates to natural or natural-like analogues of insect kinin neuropeptide [Hy]-RQKTVFSSWG-[NH2] (SEQ ID NO:2) having activity against insects, for example hemipteran, dipteran, lepidopteran and / or blattodean insects, such as aphids, moths and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.

This invention provides the application of a reagent for detecting GYSY-1 peptide in the preparation of products for assessing the prognosis and immunotherapy sensitivity of colorectal cancer, relating to the field of clinical tumor testing technology. GYSY-1 peptide is highly expressed in colorectal cancer tissues, and its expression level is positively correlated with poor overall prognosis in colorectal cancer patients, thus serving as a predictive factor for colorectal cancer prognosis. Patients with high levels of GYSY-1 peptide in colorectal cancer tissues have higher mortality rates; using it as a biomarker helps improve the positive concordance rate of colorectal cancer prognosis assessment, determining the malignancy and presence of metastasis. Furthermore, high expression of this peptide in colorectal cancer tissues is associated with lower CD8-positive cell infiltration, PD-L1 expression levels, and PD-1 / PD-L1 immune checkpoint therapy sensitivity in colorectal cancer samples; using it as a biomarker can be used to assess immunotherapy sensitivity and predict the efficacy of PD-1 / PD-L1 inhibitors.

This invention discloses a lethal gene related to soybean thrips, MuαCOPI, and its application in the control of soybean thrips. This invention provides a lethal gene related to soybean thrips, MuαCOPI. Feeding soybean thrips with the dsRNA targeting MuαCOPI significantly increases the mortality rate. Further analysis using a combination of dsMuαCOPI and Serratia marcescens to feed soybean thrips resulted in a corrected mortality rate of approximately 88% for second-instar nymphs and approximately 52% for adults within 7 days. Synergistic virulence index analysis showed that the combined treatment of second-instar nymphs and adults with dsMuαCOPI and HvSm-1 produced a synergistic effect in virulence. This invention provides a safe and efficient method for the control of soybean thrips, with promising application prospects.

The invention belongs to the field of biology, and discloses chitinase, the amino acid sequence of which is as shown in SEQ ID NO.1. The chitinase can significantly destroy the throat structure of the nematode to cause nematode death, so that the death rate of the nematode is significantly increased; meanwhile, the invention further discloses a preparation method and application of the chitinase.

Disclosed are a pharmaceutical composition for animals containing 5-hydroxy-1-methylimidazolidine-2,4-dione as an active ingredient, more specifically, a composition for preventing or treating kidney disease in animals containing 5-hydroxy-1-methylimidazolidine-2,4-dione or a pharmaceutically acceptable salt thereof as an active ingredient, a method of treating kidney disease in animals using the compound, and a functional feed for preventing or ameliorating kidney disease in animals containing the compound as an active ingredient. The 5-hydroxy-1-methylimidazolidine-2,4-dione can effectively ameliorate and treat symptoms of kidney disease when administered to companion animals such as dogs or cats with kidney disease. Therefore, there are provided a novel agent and method for treating kidney disease of animals that can effectively treat kidney disease, which is the cause of high mortality in companion animals.

This invention belongs to the field of water treatment and discloses a sludge treatment antibacterial conditioner, its preparation method, and its application. The conditioner improves sludge dewatering performance by adding polyaluminum ferric silicate as an inorganic flocculant for charge neutralization and destabilization, and adsorption bridging; it also incorporates plant enzymes to destroy bacterial structures for sterilization; and wood ash and straw form a rigid skeletal support structure. This invention forms a triple sterilization defense line, achieving high kill rates of common pathogens and high parasite egg mortality. Through the charge neutralization of polyaluminum ferric silicate, the cell wall disruption of extracellular polymers by compounded plant enzymes, the skeletal support system constructed by wood ash and straw, and the multiple dewatering processes via polyquaternary ammonium salt adsorption bridging, the sludge moisture content is reduced, lowering the costs of subsequent sludge transportation, disposal, and utilization. Using natural plant extracts and agricultural waste as the main functional components replaces components in traditional chemical conditioners that easily produce toxic byproducts, the treated sludge can be directly used for land application or landscaping, making it environmentally friendly.

The present application relates to the field of agricultural biotechnology, and particularly relates to a plant-mediated Heliothis armigera TaSUC2 gene VIGS silencing system and application thereof. The present application relates to a virus-induced gene silencing (VIGS) technology for modifying a host plant, so that the host plant can stably express a dsRNA fragment of a Heliothis armigera TaSUC2 gene, and through the Heliothis armigera feeding on the host plant, the target gene in the Heliothis armigera is cross-border silenced, so that the size of the Heliothis armigera is reduced, the mortality of the Heliothis armigera is significantly improved, the delivery efficiency of the dsRNA is effectively improved, and a new method is provided for a green and sustainable prevention and control strategy of the Heliothis armigera.