10results about How to "Increased mortality" patented technology

The application belongs to the field of agricultural biotechnology, and particularly relates to a dsRNA of a brown planthopper gene and application of the dsRNA in preventing and treating the brown planthopper and related methods. NlUGT7 The dsRNA of the gene is prepared through in vitro transcription, and RNA interference treatment is performed on the brown planthopper through microinjection. NlUGT7 The results show that after the dsRNA is injected, the expression amount of the gene in the brown planthopper of biotype Y and biotype 1 is significantly reduced. NlUGT7 After the gene is silenced, the survival rate of the brown planthopper of biotype Y feeding on YHY15 rice containing a Bph15 resistance gene or the brown planthopper of biotype 1 feeding on TN1 rice not containing the resistance gene is significantly reduced. NlUGT7 The gene plays an important role in the survival process of the brown planthopper, and can be used as an effective target for preventing and treating the brown planthopper. NlUGT7 The gene and the dsRNA thereof provided by the application can be applied to research and development of biological pesticides for the brown planthopper and green prevention and control, and have good application prospects.

The invention belongs to the technical field of biological medicines, and particularly relates to application of an mTOR inhibitor to improvement of sleep disorders. Aiming at the defects of the existing hypnotics, the invention finds that the sleep disorder can be improved by using the Rapamycin through researching a new sleep pathway target. The Rapamycin disclosed by the invention is a non-addictive sleeping drug, and can induce natural sleep behaviors; compared with an existing clinical hypnotics which has the side effect of greatly increasing the death rate, the Rapamycin administration disclosed by the invention is beneficial to improving the sleep quality and prolonging the life of animals at the same time. The invention discloses a remarkable effect of Rapamycin in the aspect of improving the sleep disorder, and the discovery has important significance in improving the sleep quality of a patient and preventing the progress of diseases, so that the Rapamycin has a wide medical prospect and a remarkable economic value.

This invention provides the application of the citrus psyllid salivary gland gene DcPS2 in the control of citrus psyllids and Huanglongbing (HLB). The nucleotide sequence of the citrus psyllid salivary gland gene DcPS2 is shown in SEQ ID NO. 1. This invention introduces dsDcPS2 into psyllids and measures the effect of DcPS2 gene silencing on psyllid survival. Experimental results show that after DcPS2 gene silencing, the mortality rate of citrus psyllids significantly increased compared to the control dsGFP, which has significant value for the control of citrus psyllids and the cultivation of psyllid-resistant plants.

This invention discloses a method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron, belonging to the field of biotechnology. The method includes: a) after acclimatizing 4-5cm largemouth bass to a normal diet for one week, the normal diet is replaced with a FeSO4·7H2O-supplemented diet (FeSO4·7H2O supplementation amount: 800-3200 mg / kg), while the control group continues to be fed a normal diet; b) after feeding the FeSO4·7H2O-supplemented diet for 14-56 days, the mortality rate from Aeromonas hydrophila infection increases, indicating successful establishment of the largemouth bass model susceptibility to pathogens. This method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron has advantages such as simple operation, high success and survival rates, good stability, and obvious pathological characteristics.

The application belongs to the field of biological medicine, and discloses a pharmaceutical composition of water channel protein inhibitor combined with temozolomide for treating brain glioma, wherein the pharmaceutical composition comprises AQP4 inhibitor AER270 and TMZ. The application further discloses a pharmaceutical preparation comprising the above pharmaceutical composition and application of the pharmaceutical preparation in preparation of a medicine for treating brain glioma. The composition provided by the application provides an effective drug combination strategy for treating brain glioma, and it is found for the first time that AER270 and TMZ have a synergistic effect on the treatment of brain glioma.

The invention belongs to the field of biological medicine, and discloses a pharmaceutical composition combining an aquaporin inhibitor and temozolomide for treating brain glioma, and the pharmaceutical composition comprises an AQP4 inhibitor AER270 and TMZ. The invention further discloses a pharmaceutical preparation containing the pharmaceutical composition and application of the pharmaceutical preparation to preparation of drugs for treating brain glioma. The composition provided by the invention provides an effective drug combination strategy for treatment of brain glioma, and it is found for the first time that the combination of AER270 and TMZ has a synergistic effect on treatment of brain glioma.

This invention provides the application of a reagent for detecting GYSY-1 peptide in the preparation of products for assessing the prognosis and immunotherapy sensitivity of colorectal cancer, relating to the field of clinical tumor testing technology. GYSY-1 peptide is highly expressed in colorectal cancer tissues, and its expression level is positively correlated with poor overall prognosis in colorectal cancer patients, thus serving as a predictive factor for colorectal cancer prognosis. Patients with high levels of GYSY-1 peptide in colorectal cancer tissues have higher mortality rates; using it as a biomarker helps improve the positive concordance rate of colorectal cancer prognosis assessment, determining the malignancy and presence of metastasis. Furthermore, high expression of this peptide in colorectal cancer tissues is associated with lower CD8-positive cell infiltration, PD-L1 expression levels, and PD-1 / PD-L1 immune checkpoint therapy sensitivity in colorectal cancer samples; using it as a biomarker can be used to assess immunotherapy sensitivity and predict the efficacy of PD-1 / PD-L1 inhibitors.

This invention discloses a lethal gene related to soybean thrips, MuαCOPI, and its application in the control of soybean thrips. This invention provides a lethal gene related to soybean thrips, MuαCOPI. Feeding soybean thrips with the dsRNA targeting MuαCOPI significantly increases the mortality rate. Further analysis using a combination of dsMuαCOPI and Serratia marcescens to feed soybean thrips resulted in a corrected mortality rate of approximately 88% for second-instar nymphs and approximately 52% for adults within 7 days. Synergistic virulence index analysis showed that the combined treatment of second-instar nymphs and adults with dsMuαCOPI and HvSm-1 produced a synergistic effect in virulence. This invention provides a safe and efficient method for the control of soybean thrips, with promising application prospects.

This invention belongs to the field of water treatment and discloses a sludge treatment antibacterial conditioner, its preparation method, and its application. The conditioner improves sludge dewatering performance by adding polyaluminum ferric silicate as an inorganic flocculant for charge neutralization and destabilization, and adsorption bridging; it also incorporates plant enzymes to destroy bacterial structures for sterilization; and wood ash and straw form a rigid skeletal support structure. This invention forms a triple sterilization defense line, achieving high kill rates of common pathogens and high parasite egg mortality. Through the charge neutralization of polyaluminum ferric silicate, the cell wall disruption of extracellular polymers by compounded plant enzymes, the skeletal support system constructed by wood ash and straw, and the multiple dewatering processes via polyquaternary ammonium salt adsorption bridging, the sludge moisture content is reduced, lowering the costs of subsequent sludge transportation, disposal, and utilization. Using natural plant extracts and agricultural waste as the main functional components replaces components in traditional chemical conditioners that easily produce toxic byproducts, the treated sludge can be directly used for land application or landscaping, making it environmentally friendly.

The present application relates to the field of agricultural biotechnology, and particularly relates to a plant-mediated Heliothis armigera TaSUC2 gene VIGS silencing system and application thereof. The present application relates to a virus-induced gene silencing (VIGS) technology for modifying a host plant, so that the host plant can stably express a dsRNA fragment of a Heliothis armigera TaSUC2 gene, and through the Heliothis armigera feeding on the host plant, the target gene in the Heliothis armigera is cross-border silenced, so that the size of the Heliothis armigera is reduced, the mortality of the Heliothis armigera is significantly improved, the delivery efficiency of the dsRNA is effectively improved, and a new method is provided for a green and sustainable prevention and control strategy of the Heliothis armigera.